Download or read book Inheritance of Random Amplified Polymorphic DNA RAPD Molecular Marker in Brown Planthopper Nilaparvata Lugens written by A. Latif and published by . This book was released on 1999 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: RAPD molecular markers were tested for segregation in two families of the brown planthopper, N. lugens. Single pair matings between individuals from two sympatric populations of N lugens, one from rice and the other from Leersia hexandra, a weed grass were used to produce the families. Four short RAPD primers amplified 41 bands (markers) in these two families. Twenty one of the markers could not be tested because no segregation was observed while the other 20 markers showed segregation in the progenies of the two families and were tested for conformity to simple Mendelian ratios. Twenty segregating markers showed ratios in conformity with them being dominant Mendelian genetic traits.
Download or read book Use of Random Amplified Polymorphic DNA RAPD Markers for Genetic Analysis of Lilium Parryi a Rare Arizona Plant written by E. Friar and published by . This book was released on 1996 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Randomly Amplified Polymorphic DNA RAPD Markers in Labeo Fimbriatus Blach written by SUDHIR S and published by . This book was released on 2000 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Random Amplified Polymorphic DNA RAPD Analysis of Genetic Variation Among Appalachian Spring Populations of the Amphipod Gammarus Minus written by Beth Ann Parker and published by . This book was released on 2000 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Variation of Random Amplified Polymorphic DNA RAPD Markers in Pecan Carya Illinoensis written by Michelle Imbalzano and published by . This book was released on 1993 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Evaluation of Fluorescent Randomly Amplified Polymorphic DNA FRAPD as a Tool for Identifying Species Hybrids and the Application of These Markers to Questions of Hybridization in Two Groups of Ohio River Basin Fishes written by Michael G. Sovic and published by . This book was released on 2011 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Randomly amplified polymorphic DNA (RAPD) has a number of characteristics that make it an attractive option for studies in hybrid zones. However, concerns regarding the repeatability of RAPD data have caused many researchers to opt for other types of genetic markers. In this dissertation, a series of modifications were applied to traditional (RAPD) methodology, and the modified RAPD markers were evaluated for their utility as a tool to identify species hybrids. Levels of repeatability were evaluated for these markers, and the estimated error rates fell within the levels of those generally accepted for studies employing dominant genotyping methods. The repeatability estimates were then incorporated into simulations to directly assess the power of dominant genetic markers in identifying various classes of parental and hybrid individuals. The level of performance varied with the number of loci analyzed, the error rates associated with the loci, and the levels of diversity between the parental taxa. However, many scenarios were identified that are likely to be encountered in natural populations in which the simulated markers performed very well, suggesting that the modified RAPD markers may serve as a viable option in many hybridization studies. Finally, two case studies are presented in which the modified RAPD markers are applied to address questions of hybridization in freshwater fishes of the Ohio River basin. In the first case study, hybrid saugeye (Sander vitreus x Sander canadensis), which are artificially produced and stocked into waters throughout Ohio, were evaluated to determine whether reproduction occurs by these fish. No evidence of reproduction was observed in the inland reservoirs of Ohio, suggesting that reproduction among F1 hybrid saugeye occurs very rarely, or not at all. However, evidence of reproduction by saugeye is presented from Sander spp. individuals collected from the Ohio River, where native populations of both parental species occur. In the second case study, fishes of the genus Carpiodes are examined in order to determine whether hybridization occurs in this group. Evidence of hybridization is presented for these fishes, and a combination of molecular data and morphological data suggest that the rates of hybridization may vary among different sympatric communities of Carpiodes spp. in the Ohio River basin. The direction of interspecific gene flow resulting from the hybridization also appears to be biased, with C. cyprinus sending genes into C. carpio more often than the reverse. The results of each of these two case studies are discussed in the context of their potential influences on the evolutionary trajectory of these groups. Finally, as part of the analysis of hybridization in Carpiodes, C. cyprinus was identified as a nonmonophyletic taxon according to mitochondrial cytochrome b sequence data. The patterns of genetic diversity observed at this gene suggest the potential that cryptic diversity occurs within C. cyprinus. Specifically, specimens collected from the Susquehanna River basin may be distinct from those in the Great Lakes and Ohio River basins. These data suggest that a reevaluation of the taxonomy of this group is warranted.
Download or read book Genetic Diversity and Differentiation in Douglas fir from RAPD Markers of Nuclear and Mitochondrial Origin written by Jan Erik Aagaard and published by . This book was released on 1997 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: In order to better understand the evolutionary patterns revealed by molecular markers, we studied genetic diversity and differentiation of populations and races of Douglas-fir using randomly amplified polymorphic DNA (RAPD) markers of nuclear and mitochondrial origin. We conducted a range-wide survey of RAPD diversity and differentiation, and compared RAPDs and published allozyme data at the population and race level. Southern hybridization using enriched organelle DNAs as probes was used to screen RAPDs for cytoplasmic DNA markers, allowing us to distinguish between RAPD bands of nuclear, mitochondrial, and putative chloroplast origin. RAPD markers of mitochondrial origin were found to be frequent among Douglas-fir RAPD profiles. In our range-wide survey, nearly half (45 %) of all polymorphic RAPDs scored exhibited maternal inheritance; the chioroplast genome is known to exhibit paternal inheritance in Douglas-fir. Hybridization of RAPD blots with mitochondrial genome enriched DNA identified 16% of a total of 237 RAPD fragments as being of mitochondrial origin. Failure to distinguish between nuclear and mitochondrial RAPD origins could seriously bias estimates of population genetic parameters. When a nuclear origin was assumed for all bands, differentiation among populations and races was nearly 1.5-fold greater then when only nuclear RAPDs were analyzed (GST=O.47 and 0.34, respectively). We infer that the large number of mitochondrial RAPD markers we observed are indicative of a large, complex, and polymorphic mitochondrial genome in Douglas-fir. Nuclear RAPD markers were considerably more polymorphic within populations than mitochondrial RAPDs (Hs=O.22 and 0.03, respectively), however mitochondrial RAPDs were much more highly differentiated than nuclear RAPDs (GSTO. 91 and 0.34, respectively). Both markers indicated similar phylogenetic relationships among populations and races. Estimates of diversity and differentiation were higher for RAPDs than for allozymes, though not significantly so (P>0.05; H5O.22 and 0.16, HT=O.32 and 0.22, and G1=0.34 and 0.29, respectively, for RAPDs and allozymes). However, simulations showed that Hs and HT for dominant biallelic markers such as RAPDs are expected to be approximately half that of a codominant, multiallelic marker with equivalent levels of underlying molecular diversity to that of our allozyme data set. This suggests that RAPDs reflect considerably higher amounts of diversity at the molecular level than do allozymes. The simulations also suggest that our estimates of population differentiation (GST) for RAPD markers are essentially unbiased at the sample sizes used.
Download or read book Advances in Rice Genetics written by Gurdev S. Khush and published by Int. Rice Res. Inst.. This book was released on 2003 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and breeding of agronomic traits. Genetic diversity, evolution, and alien introgression. Molecular markers, QTL mapping, and marker-assisted selection. Genomics. Gene isolation and function. Tissue culture and transformation. Genetics of rice pathogens.
Download or read book Plant Breeding Abstracts written by and published by . This book was released on 1999 with total page 1132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Resistance to Arthropods written by C. Michael Smith and published by Springer Science & Business Media. This book was released on 2006-01-16 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book synthesizes new information about the environmental advantages of plant resistance, transgenic resistance, the molecular bases of resistance, and the use of molecular markers to map resistance genes. Readers are presented in-depth descriptions of techniques to quantify resistance, factors affecting resistance expression, and the deployment of resistance genes. New information about gene-for-gene interactions between resistant plants and arthropod biotypes is discussed along with the recent examples of using arthropod resistant plants in integrated pest management systems.
Download or read book Microbiology written by and published by . This book was released on 1995 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biological Agricultural Index written by and published by . This book was released on 1997 with total page 2984 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agrindex written by and published by . This book was released on 1995 with total page 822 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1999 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Brown Planthopper written by International Rice Research Institute and published by Int. Rice Res. Inst.. This book was released on 1979 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Planthoppers written by Kong Luen Heong and published by Int. Rice Res. Inst.. This book was released on 2009 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Markers in Plants written by Robert J. Henry and published by John Wiley & Sons. This book was released on 2012-09-27 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Markers in Plants surveys an array of technologies used in the molecular analysis of plants. The role molecular markers play in plant improvement has grown significantly as DNA sequencing and high-throughput technologies have matured. This timely review of technologies and techniques will provide readers with a useful resource on the latest molecular technologies. Molecular Markers in Plants not only reviews past achievements, but also catalogs recent advances and looks forward towards the future application of molecular technologies in plant improvement. Opening chapters look at the development of molecular technologies. Subsequent chapters look at a wide range of applications for the use of these advances in fields as diverse as plant breeding, production, biosecurity, and conservation. The final chapters look forward toward future developments in the field. Looking broadly at the field of molecular technologies, Molecular Markers in Plants will be an essential addition to the library of every researcher, institution, and company working in the field of plant improvement.
