Download or read book Influence of the Titanium Dental Implants Surfaces on Viability of Osteoblast like Cells written by Oliveira Natacha Kalline and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Osseointegration was defined as a structural and functional connection between a living tissue-bone and the surface of an implant subjected to a functional load. One of the current chalenges of osseointegration is the development of a titanium implant surface that could accelerate or improve osseointegration process. Strategies to achieve this issue include adjustments in surface characteristics like roughness, chemical composition, superficial energy and hydrophilicity that can affect cell behavior in bone-implant interface.The aim of this in vitro study was to compare the response of osteoblast-like cells grown to four different dental implant surfaces.Four different dental implant surfaces were tested: sandblasted with acid-etched and calcium phosphates coating (AAMB); doubleacid-etched (SW); acid-etched (MP), and anodized (VA). Osteoblast-like cells (Osteo 1) were seeded on the top of these implantsurfaces (5x104 cells/implant). The viability of the cells was assessed at 24, 48 and 72 hours later, using the MTT reduction assay.The distribution and morhology of the cells was observed in scanning eletron micrographs. Experiments were carried out in triplicate. The cell viability was similar during whole experimental times in all surfaces, except by the double acid-etched surface. At 72 hoursafter plating cells grown on the top of the SW surface presented the lowest viability (p = 0.03). Most surface area was covered by thecells, which exhibited the classical poligonal and filipopia-rich osteoblat-like morphology in all surfaces, except in the SW surface. Thissurface was mostly devoid of cells, which were elongated with a flat interface with the implant surface.The double acid-etching treatment of dental implant surfaces creates a morphlogy that impairs the intimate interface with culturedosteoblast-like cells. This could have a negative impact in the osseointegration process in vivo.

Download or read book Osteoblast Cell Response to Titanium Dental Implant Surfaces Treated with Different Chemical Agents written by Masa Roland and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Osteoblast cell response to titanium dental implant surfaces treated with different chemical agentsAbstractRelatively high survival rates of dental implants encourage clinicians to use this type of oral rehabilitation frequently in their daily practice. However, implant failures are increasing parallel with the number of implant placements. Bacterial colonization is one of the main causes of periimplant inflammation. In the therapy of both periimplant mucositis and periimplantitis mechanical debridement and chemical decontamination are fundamental steps.1 In order to be up to date in the fight against bacteria, various types of antibacterial solutions should be used during pocket irrigation. However, they should undergo through different in vitro and in vivo tests before daily use. In this study the response of primary osteoblast cells to chemically treated titanium surfaces was examined. Different chemical and cell viability tests, and for visualization fluorescent staining were carried out.Background In case of peri-implant infections, the main goals of treatment are to decontaminate the dental implant surface in order to reduce the bacterial load and to facilitate reintegration. Various chemical agents have been proposed for surface decontamination in the therapy of peri-implantitis. However these antibacterial agents could alter the surface properties of the implant material and also the osteoblast response.Aim/HypothesisOur aim was to evaluate the interaction of titanium dental implant surface with three different antibacterial solutions: chlorhexidine, povidone-iodine and chlorine dioxide. Our null hypothesis was that residues of these agents do not modify either the implant surface or the osteoblast response.Materials and MethodsCommercially pure (CP4) sandblasted, acid-etched titanium sample discs (Dentiu00ae System Ltd., Hungary) 1.5 mm thick and 9 mm in diameter, rinsed with ultrapure water, were used as control surfaces. Three different chemical agents were investigated: chlorhexidine digluconate (Curasept ADS 220, 0.2%, Switzerland), povidone-iodine (Betadine, 10 %, Switzerland) and chlorine dioxid solution (Solumium, 0.12%, Hungary). Titanium sample discs were treated for 5 minutes in each case. Wettability studies were performed by an OCA 20 (Dataphysics, Filderstadt, Germany) contact angle measuring device with water and diiodomethane drops. Surface free energy (SFE) was determined according to Owen-Wendt-Rabel-Kaelble (OWRK) method. The chemical compositions of the Ti discs were studied by X-ray photoelectron spectroscopy (XPS). In vitro attachment (24h) and proliferation (72h) of primary osteoblast cells were investigated via dimethylthiazol- diphenyltetrazolium bromide (MTT) and fluorescence microscopy.ResultsContact angle measurements revealed significant differences in SFE of the treated discs compared to the control Ti surface. Povidone-iodine treated samples had the lowest contact angles and the highest SFE. Chlorhexidine (CHX) - and chlorine dioxide (ClO2) - treated discs showed significantly higher contact angle values compared to control Ti samples. XPS confirmed the constant presence of typical surface elements (Ti, O,C,N) on each surface, while chlorine was detected only in the ClO2 group. MTT test proved the viability of primary osteoblast cells after chemical agent treatment. In spite of the significant difference in surface free energy, and the chlorine presence in the ClO2 group, MTT assay indicated similar osteoblast cell attachment (24h) in the three study groups and the control groups (plate and Ti). After 72h, a significant increase was observed in cell numbers (high proliferation rate) in all of the investigated groups compared to cell numbers after 24h, no significant difference was found beetwen the groups. Fluorescent images confirmed these results. Conclusion and Clinical implicationsIn spite of the different wettabilities, primary osteoblast cells showed similar response and high viability on each of the surfaces. Therefore, it seems no far-fetched to assume that none of the irrigating agents would have a negative influence on osseointegration. In conclusion, we suggest the use of PVPI and high purity ClO2-solution beside the gold standard CHX as implant surface decontaminating agents, in order to reach broader antibacterial efficiency. Key words: peri-implantitis, chlorhexidine, povidone-iodine, chlorine dioxide, osteoblast, titanium dental implantAcknowledgmentsThe work was supported by the grant GINOP-2.3.2-15-2016-00011 to a consortium led by the University of Szeged, Szeged, Hungary (participants: the University of Debrecen, Debrecen, and the Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary).

Download or read book Implant Surfaces and their Biological and Clinical Impact written by Ann Wennerberg and published by Springer. This book was released on 2015-01-05 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the reader with the knowledge required in order to understand the chemical, physical, mechanical, and topographical aspects of implant surfaces, as well as their impact on the biological response. Common ways to modify implant surfaces are described, and methods for the evaluation of surface properties are presented in an easy-to-read style. Experimental results that have contributed to surface modifications relevant for commercial available implants are presented, with emphasis on in vivo and clinical studies. While the focus is primarily on surface modifications at the micrometer and nanometer levels, alterations at the millimeter level are also covered, including thread designs and their possible influence on stress distribution. In addition, it is analyzed how surface alterations have changed the clinical long-term results for certain groups of patients.

Download or read book Bone Implant Interface written by Hugh U. Cameron and published by St. Louis ; Toronto : Moseby. This book was released on 1994 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Effects of Surface Modified Titanium Implants on Osseointegration in Irradiated Bone written by Junyuan Li and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Effects of Surface-modified Titanium Implants on Osseointegration in Irradiated Bone" by Junyuan, Li, 黎俊媛, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Radiotherapy is a common treatment for head and neck cancers. However, it compromises bone healing. Titanium implanthas been shown to be a predictable method for replacing missing teeth. Clinical studies revealed that implant failure rate in irradiated regionwas high. Many studies showed that modifications of implant surface could enhance implant osseointegration by improving cell attachment, cell growth and bone formation. Nevertheless, there were few studies investigating the effect of implant surface modification on osseointegration in irradiated bone. In the first experiment, the effect of fluoride-modified (FM) titanium surface on irradiated osteoblast attachment was assessed. The morphology and chemical composition of FM surface was assessed by SEM, AFM and XPS. Osteoblasts received 0Gy, 2Gy, 4Gy, 6Gy, 8Gy, 10Gy radiation. Cell number, fluorescence intensity and cell area of irradiated osteoblasts were assessed. The number of osteoblasts onFM surface was fewer than those on NF surface after 0Gy, 2Gy, 8Gy and 10Gy radiation. Cell area of osteoblasts on FM surface was less at 2Gy radiation but larger at 6Gy radiation than on NF surface. The fluorescence intensity of osteoblasts was also higher on NF surface than on FM surface after receiving 0Gy, 2Gy, 4Gy, 10Gy radiation. In the second experiment, an animal model was established to study the effect of radiation on osseointegration. Rabbits were divided into 15Gy and 30Gy radiation groups. Only the left leg was exposed to radiation, and the right leg was protected from radiation. Totally, 24 implants were inserted. Implant stability quotient (ISQ), bone volume to total volume (BV/TV), bone-to-implant contact (BIC), and bone growth rate were measured. After 15Gy and 30Gy of radiation, ISQ and BV/TV were significantly reduced. At week 3, 15Gy radiation group displayed slower bone growth rate comparing with the control side. Fluorochrome results showed that the 30Gy radiation side had a significantly slower apposition of new bone.In addition, BIC on30Gy radiation side was notablypoorer than that on 15Gy radiation side and on 30Gy control side. Based on the animal model, the third experiment investigated effects of calcium phosphate nanocrystals on implant osseointegration in irradiated bone. Titanium implants treated with nano-scale calcium phosphate (CaP) crystals served as the test group while ones with dual acid-etching only served as the control group. The left leg of rabbits received 15Gy radiation and implants were placed in the irradiated leg. Significant higher ISQ was detected in the nano-CaP group at week 12. The bone growth rate in nano-CaP group was more than doubled than the control group at both week 6 and week 9. The fourth experiment evaluated artifacts on micro-CT images caused by titanium dental implant. Implants were assigned into four groups: (1) implant only; (2) implant with covering screw; (3) implant with resin embedding; and (4) implant with covering screw and resin embedding. Each implant was scanned by micro-CT at 3 angulations. Implant angulation was the most determining factor followed by resin embedding. Minimal metallic artifacts were obtainedin non-embedded implants with its axis paralleling to X-ray. DOI: 10.5353/th_b5312315 Subjects: Osseointegration Dental implants

Download or read book The Effect of Titanium Implants on the Peripheral Circadian Rhythm of Bone written by Kirstin Ty McCarville and published by . This book was released on 2013 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: BACKGROUND: Endosseous implant osseointegration has been primarily studied from a wound healing-model. The molecular pathway of implant osseointegration is not known. Previous animal studies have shown vitamin D insufficiency impairs the establishment of implant osseointegration. Recent microarray data found an interesting connection between vitamin D receptor and circadian rhythm genes using a vitamin D insufficient animal model (implant failure model). Per1 is one of eight circadian genes that has widely been used as a molecular marker of circadian rhythm. Previous experiments with forskolin-synchronized expression of Per1 in bone marrow mesenchymal stromal cells (BMSCs) in vitro showed vitamin D supplementation significantly and dose dependently increased the baseline expression of Per1::luc, but preserved the circadian rhythm. We have thus postulated that the circadian rhythm of BMSCs may be influenced by titanium dental implants. OBJECTIVES: To determine the effect of titanium implant substrates with different surface characteristics on BMSCs peripheral circadian rhythm. METHODS: In this study, a series of in vitro studies were designed to characterize the peripheral circadian rhythm of BMSCs harvested from Per1::luc transgenic Wistar rats. Per1 transgenic rats genomic locus of Per1 allele were modified by inserting a beetle luciferase reporter gene (Per1::luc). The peripheral circadian rhythms over a 24-hour period were monitored by the real time activation of luciferase-mediated bioluminescence. Lumicycle was the instrument used to record the luciferase activity (Per1 expression) every ten minutes over five days. Data was evaluated both in the raw form (photons/second) Per1 gene expression and baseline subtracted data. The baseline subtracted data provides the peaks, troughs and periods of the BMSCs peripheral circadian rhythm over 5 days. The first experiments were completed on plastic cell culture dishes with and without vitamin D supplementation. In the second project, two titanium substrates with polished (smooth) surface or complex (rough) surface with blasted, acid etched and discrete HA crystalline deposition were characterized. Surface topography: Sa (average surface roughness) and Sdr (hybrid parameter that presents information about the number and height of peaks of a given surface) were determined by a 3D surface profilometry. To ensure BMSC survival and proper attachment a WST-1 assay was completed. To determine the level of osteoblast differentiation capabilities of BMSCs on all three surfaces (plastic, smooth and rough) a calcium mineralization assay was preformed. In the third project, BMSC peripheral circadian rhythm studies were then executed on smooth and rough titanium surfaces with and without vitamin D supplementation. This study was accomplished using Per1:;luc BMSCs and Lumicyle. In the forth project, Taqman- based quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to validate gene expression of 6 circadian rhythm-related molecules (Per1, Per2, Id2, Bmal1, Clock, and NPas2). RESULTS: Bioluminescent raw measurement of Per1 expression on plastic with and without vitamin D showed that vitamin D increased Per1 expression over days 2, 3 and 4. The baseline subtracted data suggested that vitamin D suppressed the amplitude (peak to trough) but maintained the period. The WST-1 assay revealed that over 4 days the three different surfaces had a comparable number of viable cells. The calcium assay also suggested that the different substrate surfaces did not have large influence on osteogenic differentiation of BMSCs. The raw measurement of Per1::luc on titanium implant substrates indicated that the rough titanium surface had a significant reduction in Per1 expression (more than 50%) of photons per second in comparison to the smooth surface. Furthermore, rough titanium with vitamin D had a lower Per1 expression when compared to the rough titanium with no vitamin D. The smooth titanium baseline subtracted data showed a consistent rhythm having developed peaks and troughs. The most dramatic difference was seen in the rough surface baseline subtracted data. The rough surface almost completely eliminated the amplitude and period of the peripheral circadian rhythm. RT-PCR validated that five of the six circadian rhythm genes were downregulated by the rough titanium surface. The only gene upregulated on rough titanium was NPAS2. CONCLUSION: Titanium material had a negative effect on Per1 expression and amplitude of BMSCs peripheral circadian rhythm. Osseointegration pathway is unknown, however, the peripheral circadian rhythm of BMSCs may play a role in the establishment of implant-bone integration. NPAS2 was found to be highly expressed with the rough titanium substrate. Although speculative, the modulation of peripheral circadian rhythm may lead to successful osseointegration.

Download or read book Surface Engineering and Technology for Biomedical Implants written by Yoshiki Oshida and published by Momentum Press. This book was released on 2014-04-15 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book synthesizes a wide range of interdisciplinary literature to provide the state-of-the art of biomedical implants. It discusses materials and explains the three basic requirements for implant success from a surface engineering perspective: biological compatibility, biomechanical compatibility, morphological compatibility. Biomedical, mechanical, and materials engineers will find this book indispensable for understanding proper treatment of implant surfaces in order to achieve clinical success. Highlights include: • Coverage of surface engineering of polymer, metallic, ceramic and composite implant materials; • Coverage of chemical, mechanical, physical, thermal, and combined surface modification technologies; • Explanations of interfacial reaction between vital tissue and non-vital implant surface; and • Methodologies and technologies for modification of surface layer/zone to promote the osteo-integration, the ultimate success for biomedical implants in both dental and medical practice.

Download or read book Chasing White Titanium Dental Implant Surfaces Physico chemical Modifications and Interaction with Osteoblast Cells in Vitro written by Milena R. Kaluđerović and published by . This book was released on 2018* with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Effect of Anodized and Hydrothermal treated Titanium Implant Surfaces on Osteoblast Precursor Cell Response written by Raymond Michael Rodriguez and published by . This book was released on 2003 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Surface Modification of Titanium Dental Implants written by Karan Gulati and published by Springer Nature. This book was released on 2023-02-27 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive technical and scientific overview of the surface modification of titanium dental implants. Coverage ranges from basic concepts of surface modification to advanced micro- and nano-engineering strategies employed to achieve augmented bioactivity to meet the needs of compromised patient conditions. A special focus of the book is advanced state-of-the-art electrochemically anodized nanostructures fabricated on implants towards enhanced bioactivity and local therapy. Surface Modification of Titanium Dental Implants will keep you current in the domain of titanium dental implants and will provide an improved understanding of their performance and application. The book will benefit engineers, clinicians, and researchers in biomaterials, biomedical engineering, dental and bone implants, nano-engineering, and technology.

Download or read book Tissue integrated Prostheses written by Per-Ingvar Brånemark and published by Quintessence Publishing (IL). This book was released on 1985 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: " This book provides the prosthodontist and oral surgeon for the first time with a safe, predictable, and scientifically proven method for permanently anchoring artificial tooth abutments in the jawbones. It combines the osseointegration research results of biologists, physicists, bioengineers, oral surgeons, and prosthodontists to demonstrate step-by-step how to achieve long-term osseointegration of dental implants" -- Amazon.

Download or read book Detoxification of Titanium Implant Surfaces Evaluation of Surface Morphology and Pre osteoblast Cell Compatibility written by Deepthi Ramesh and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Success of a dental implant is primarily assessed by its surface condition and its capability to biologically integrate with surrounding soft and hard tissues. When the surface of an implant is compromised by bacterial adhesion, it can result in development of peri-implantitis and ultimately implant loss. One of the primary etiological factors resulting in peri-implantitis is the formation of a biofilm created by adhesion of bacteria on implant surfaces. Peri-implantitis is a site-specific disease that causes bone loss and inflammation around a functional implant. Clinicians commonly use a combination of mechanical debridement/detoxification methods with acidic chemicals to remove adhered biofilm. It is hypothesized that acidic conditions caused by these detoxification chemicals, in addition to mechanical abrasion, can lead to surface changes including pitting, corrosion and discoloration, which can affect the growth of bone-forming cells. The study’s main goal was to evaluate changes in surface morphology of titanium after bacterial adhesion and detoxification procedures. In addition, proliferation and differentiation of bone-forming cells were analyzed after exposure to bacterial adhesion and detoxification procedures on implant surfaces to infer about re-osseointegration post-treatment.

Download or read book The Effects of Titanium Surfaces on the Attachment Proliferation and Morphology of Human Osteoblast like Cells written by Diane Elizabeth Gawronski and published by . This book was released on 1997 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Influence of Milled Vs Laser grooved Zirconia Implant Surfaces on Human Osteoblast Behavior written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction: Zirconia is a potential alternative to titanium for dental implants, however, there is a need for the improvement of surface treatment protocols to enhance its bioactivity. Several strategies have been described to achieve this aim, including the manufacture of surface modifications that increase roughness using laser, micro-machining or surface treatments as sandblasting and acid etching (SB-AE). The benefits of laser graving versus milling are not yet known.Objective: In vitro comparison of osteoblast response to Zirconia with SB-AE surfaces and additional groove-textures produced by micro-machining or laser, regarding cell attachment, cell proliferation and differentiation.Materials and Methods: Milled (M-YTZP) and laser (L-YTZP) groove textured zirconia discs with 10, 100, and 100 u00b5m features of depth, width and spacing, respectively, were produced using press and sintering. Untextured (S-YTZP) discs were used as controls. SB-AE protocol was applied to all samples to achieve a mean roughness ranging between 2.49 u00b1 0.3u00b5m and 2.03 u00b1 0.48u00b5m (p>0.05). Human osteoblasts were cultured on discs for 14 days. Morphology and cellular adhesion were observed using scanning electron microscopy (SEM) after 1 day. Cell viability and proliferation were evaluated at 1,3,7 and 14 days using a resazurin-based method. Alkaline phosphatase (ALP) activity was evaluated at 7 and 14 days and Collagen type I, Osteopontin and IL-1u03b2 were evaluated at 3 and 7 days using ELISA. All results were presented as mean u00b1 standard deviation (SD). Group comparisons were tested using Anova (Tukeyu2019s post-hoc) using appropriate statistical software and significance was set at p0.05.Results: Cell viability and proliferation increased over time in all study groups. M-YTZP and L-YTZP viability was significantly higher than in S-YTZP group at 7 and 14 days (p

Download or read book Bone Response to Dental Implant Materials written by Adriano Piattelli and published by Woodhead Publishing. This book was released on 2016-10-27 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bone Response to Dental Implant Materials examines the oral environment and the challenges associated with dental biomaterials. Understanding different in vivo and in vitro responses is essential for engineers to successfully design and tailor implant materials which will withstand the different challenges of this unique environment. This comprehensive book reviews the fundamentals of bone responses in a variety of implant materials and presents strategies to tailor and control them. Presents a specific focus on the development and use of biomaterials in the oral environment Discusses the basic science of the dental interface and its clinical applications Contains important coverage on the monitoring and analysis of the dental implant interface

Download or read book The Effect of Pure Titanium and Titanium Alloy on Osteoblast and Chondrocyte Maturatin In Vitro written by Jack Lincks and published by . This book was released on 1999 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Implant success is dependent on the interaction of the implant with surrounding tissues. By understanding this, better devices can be developed. Substrate specific information regarding implant surface characteristics and its influence on mesenchymal cell proliferation and differentiation may be helpful in the design of implants to optimize bone growth at the interface. Based on in vivo and in vitro studies, biomaterial composition, surface energy, surface roughness, and surface topography appear to play a major role in implant success. However, in-depth analysis of cellular response to various implant surface characteristics and the ensuing local and systemic factor production remain to be performed. The first aim of this study was to evaluate the ability of two implant characteristics, chemical composition and surface roughness, to modulate various parameters of osteoblast metabolism in vitro. Commercially pure titanium (Ti) and titanium-6 wt.% aluminum-4 wt.% vanadium alloy (Ti-A) disks were machined to achieve a specific roughness through either fine-polishing (S, smooth, Ra 0.2 micronmeter) or wet sand-grinding (R, rough, Ra 3.0 micronmeter). Profilometry and scanning electron microscopy (SEM) were used to determine surface roughness, Auger spectroscopy and FT-IR were also used to characterize the disks. MG63 osteoblast-like human osteosarcoma cells were seeded at 9300 cells/cm2 and cultured to confluence on standard tissue culture polystyrene or the Ti surfaces. Cell morphology was assessed by SEM. Osteoblast cultures were examined for differences in proliferation as measured by cell number, and 3H-thymidine incorporation; differentiation by cell and cell layer alkaline phosphatase specific activity (ALpase) and osteocalcin production; matrix synthesis by proteoglycan sulfation and % collagen production; and cytokine production by transforming growth factor-Beta and prostaglandin E2 production.

Download or read book Responses of Osteoblast like Cells and Human Gingival Fibroblasts to Titanium Surfaces Treated with Ultraviolet Irradiation and Or Alendronate Immersion written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Placement of dental implants has become an essential therapeutic method for the treatment of edentulous or partially edentulous patients. Titanium has been the material of choice for dental implants because of its superior biocompatibility, chemical stability, and mechanical strength. However, bioactivities of titanium substantially decrease when exposed to ambient conditions. Hence, there have been many researches to overcome this limitation by applying various surface modifications.In this study, we aimed to evaluate the effects of ultraviolet (UV) treatment and alendronate (ALN) immersion on the proliferation and differentiation of MG-63 osteoblast-like cells and human gingival fibroblasts (HGFs) cultured on titanium surfaces. MG-63 cells were used for sandblasted, large grit, and acid-etched (SLA) titanium surfaces, and HGFs were used for ma-chined (MA) titanium surfaces. SLA and MA specimens were subdivided into four groups (n = 12) according to the combination of surface treatments (UV treatment and/or ALN immersion). After culturing MG-63 cells and HGFs on titanium discs, cellular morphology, proliferation, and differentiation were evaluated. The results revealed that UV treatment of titanium surfaces did not alter the proliferation of MG-63 cells; however, HGF differentiation and adhesion were in-creased by UV treatment. In contrast, ALN immersion of titanium discs reduced MG-63 cell pro-liferation and changed HGFs into a more atrophic form. Simultaneous application of UV treatment and ALN immersion induced greater differentiation of MG-63 cells. Within the limita-tions of this cellular level study, we showed that simultaneous application of UV treatment and ALN immersion of titanium surfaces could improve the osseointegration of titanium implants; in addition, UV treatment may be used to enhance mucosal sealing of titanium abutments.