Download or read book Influence of Proteins and Ordered Lipid Domains on Nanoparticle Interactions with Model Biomembranes written by Eric S. Melby and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of nanoparticle interaction with model biomembranes can provide important insights into the role of individual biomolecules in governing these interactions that are often not possible in studies with living cells as a result of their biomolecular complexity. That being said, it is important that we balance the simplicity that our model systems can provide with the true complexity of the systems we aim to understand. In this field researchers have focused on understanding the interaction between as-synthesized nanoparticles and model biomembranes composed of a single phospholipid or binary mixtures of phospholipids. While these studies are fundamentally important, it is critical that we simultaneously begin to expand this research in new directions by systematically evaluating how other important membrane structures, membrane biomolecules, and nanoparticle alterations after synthesis may govern nanoparticle interactions with biological systems. In the studies discussed here we have pushed the boundaries of research in this field and demonstrated the dramatic effect that phase-segregated membrane domains, peripheral membrane proteins, and nanoparticles modified by the formation of complexes with proteins can have on nanoparticle interactions with model biomembranes. Taken together these results provide insights into designing studies with nanoparticles and model biomembranes that more closely parallel the interactions between nanoparticles and true cellular membranes. This includes the need to investigate how after-synthesis nanoparticle modifications, such as complexation with proteins and natural organic matter, alter the properties of nanoparticles and ultimately their interactions with biological systems. Furthermore, it highlights the need to carefully consider all membrane biomolecules and the ways in which their presence in the membrane may guide the interactions with nanoparticles, not simply individual phospholipids. Finally, investigations of nanoparticle interaction with model biomembranes that most effectively mimic the interactions with true biological systems should be guided by collaborations that bring together experts in model biomembranes, living cells, and computational simulations. Such collaborations will result in deeper, and more rapid, insights that have the potential to deeply benefit society through the effective use of benign nanoparticles.

Download or read book Lipid Domains written by and published by Academic Press. This book was released on 2015-06-08 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. - Written by leading experts - Contains original material, both textual and illustrative, that should become a very relevant reference material - The material is presented in a very comprehensive manner - Both researchers in the field and general readers should find relevant and up-to-date information

Download or read book Protein Nanoparticle Interactions written by Masoud Rahman and published by Springer Science & Business Media. This book was released on 2013-06-24 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the fabrication of nanomaterials and exploration of their properties have attracted the attention of various scientific disciplines such as biology, physics, chemistry, and engineering. Although nanoparticulate systems are of significant interest in various scientific and technological areas, there is little known about the safety of these nanoscale objects. It has now been established that the surfaces of nanoparticles are immediately covered by biomolecules (e.g. proteins, ions, and enzymes) upon their entrance into a biological medium. This interaction with the biological medium modulates the surface of the nanoparticles, conferring a “biological identity” to their surfaces (referred to as a “corona”), which determines the subsequent cellular/tissue responses. The new interface between the nanoparticles and the biological medium/proteins, called “bio-nano interface,” has been very rarely studied in detail to date, though the interest in this topic is rapidly growing. In this book, the importance of the physiochemical characteristics of nanoparticles for the properties of the protein corona is discussed in detail, followed by comprehensive descriptions of the methods for assessing the protein-nanoparticle interactions. The advantages and limitations of available corona evaluation methods (e.g. spectroscopy methods, mass spectrometry, nuclear magnetic resonance, electron microscopy, X-ray crystallography, and differential centrifugal sedimentation) are examined in detail, followed by a discussion of the possibilities for enhancing the current methods and a call for new techniques. Moreover, the advantages and disadvantages of protein-nanoparticle interaction phenomena are explored and discussed, with a focus on the biological impacts.

Download or read book Membrane Protein Lipid Interactions Physics and Chemistry in the Bilayer written by Jordi H. Borrell and published by Springer. This book was released on 2016-03-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been conceives as a brief introduction to biomembranes physical chemistry for undergraduate students of sciences, and it is particularly dedicated to the lipid-protein membrane interactions. A general introduction is presented in Chapters 1 and 2. The following Chapters, 3 and 4, describe the most accepted theories on lipid-membrane protein interactions as well as the new experimental approaches, in particular, these arose from nano sciences as atomic for microscopy and single molecule force spectroscopy. The book emphasizes the relevance of physical parameters as the lateral surface pressure and the lipid curvature as actors for understanding the physicochemical properties of the biomembranes.

Download or read book Protein Lipid Interactions written by C.Reyes Mateo and published by Springer. This book was released on 2005-12-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.

Download or read book The Role of Lipid Domains and Sterol Chemistry in Nanoparticle cell Membrane Interactions written by Andrew B. Fuhrer and published by . This book was released on 2020 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a growing interest in the scientific research community to develop nanoparticles for use in novel commercial and biomedical applications, fueled by recent advances in nanotechnology and nanoparticle synthesis. Potential applications for nanoparticles include use as catalysts during chemical manufacturing processes, use as drug delivery vehicles and imaging agents for biomedical applications, and as surfaces for adsorption during removal of environmental pollutants. The use of nanoparticles in such applications has raised questions concerning their safety and impact on human health. Answers to these questions require a greater understanding of the interactions between nanoparticles and living cells. Models of the cell membrane have been employed to investigate how nanoparticles may adsorb to, fuse with, or penetrate the cell membrane, however careful consideration of the membrane model for such mechanistic studies is necessary. This thesis investigates the role of membrane lipid domains, which are lipid phase segregations comprised of saturated lipids and sterols, in modulating nanoparticle-membrane interactions and further explores how sterol chemistry impacts said interaction. Model membranes were synthesized with an equimolar ratio of sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and varied sterol composition to yield vesicles with varied lipid domain properties. Fluorescence anisotropy and Forster resonance energy transfer of fluorescent probes was measured to quantify the degree of ordered domain formation in model vesicles. Additionally, confocal microscopy was performed to visualize lipid domains. Following lipid domain characterization, vesicles in which a self-quenching fluorescent dye was encapsulated were exposed to plain silica nanoparticles (diameter 37.5 ± 1.8 nm) and leakage of dye was measured to determine the degree of membrane disruption. By analyzing the results of vesicle leakage assays alongside the results from domain characterization, it was concluded that the lipid domain profile of the membrane alone is not an ideal predictor of nanoparticle-membrane interactions

Download or read book Advances in Biomembranes and Lipid Self Assembly written by and published by Academic Press. This book was released on 2018-02-24 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biomembranes and Lipid Self-Assembly, Volume 27, formerly titled Advances in Planar Lipid Bilayers and Liposomes, provides a global platform for a broad community of experimental and theoretical researchers studying cell membranes, lipid model membranes, and lipid self-assemblies from the micro- to the nanoscale. The assortment of chapters in this volume represents both original research and comprehensive reviews written by world leading experts and young researchers, with topics of note in this release including TiO2 Nanomaterials as Electrochemical Biosensors for Cancer, the Reconstitution of Ion Channels in Planar Lipid Bilayers: New Approaches, and Shear-Induced Lamellar/Onion Transition in Surfactant Systems. - Surveys recent theoretical and experimental results on lipid micro- and nanostructures - Presents potential uses of applications, like clinically relevant diagnostic and therapeutic procedures, biotechnology, pharmaceutical engineering and food products - Includes both original research and comprehensive reviews written by world leading experts and young researchers - Provides a global platform for a broad community of experimental and theoretical researchers studying cell membranes, lipid model membranes and lipid self-assemblies, from micro to nanoscale

Download or read book Effects of the Nanoparticle Protein Corona on Nanoparticle cell Membrane Interaction written by Mohamad Javad Haghighat Manesh and published by . This book was released on 2020 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasing applications of nanoparticles in biomedical research and consumer products has raised concerns regarding their potential toxicity. Although the effects of nanoparticles on human health and environment are still under investigation, previous studies have revealed significant evidence of nanoparticle toxicity to cells. With the cell plasma membrane being the first entity that first interacts with nanoparticles upon possible exposure, numerous studies have focused on nanoparticle interactions with cell plasma membrane. Nanoparticles entering a biological fluid are covered by proteins which adsorb to the particle surface, commonly called the nanoparticle protein corona. Protein corona changes nanoparticle characteristics, thereby altering their biological behavior. A better understanding of how the corona regulates nanoparticle-membrane interactions can help the design of the biomedical applications of nanoparticles. This study seeks to investigate the role of the corona from human serum on nanoparticle-induced cell membrane damage. Plain silica nanoparticles, known for disrupting the lipid bilayer of the cell membrane, were used as nanoparticle models. The effects of pristine nanoparticles are recorded after interactions with model membranes and red blood cells and compared to results from nanoparticles with the protein corona on their surface.

Download or read book Study of Bacteriorhodopsin in a Controlled Lipid Environment written by Vivien Yeh and published by Springer. This book was released on 2018-10-26 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.

Download or read book Biological Soft Matter written by Corinne Nardin and published by John Wiley & Sons. This book was released on 2021-04-06 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Soft Matter Explore a comprehensive, one-stop reference on biological soft matter written and edited by leading voices in the field Biological Soft Matter: Fundamentals, Properties and Applications delivers a unique and indispensable compilation of up-to-date knowledge and material on biological soft matter. The book presents a thorough overview about biological soft matter, beginning with different substance classes, including proteins, nucleic acids, lipids, and polysaccharides. It goes on to describe a variety of superstructures and aggregated and how they are formed by self-assembly processes like protein folding or crystallization. The distinguished editors have included materials with a special emphasis on macromolecular assembly, including how it applies to lipid membranes, and proteins fibrillization. Biological Soft Matter is a crucial resource for anyone working in the field, compiling information about all important substance classes and their respective roles in forming superstructures. The book is ideal for beginners and experts alike and makes the perfect guide for chemists, physicists, and life scientists with an interest in the area. Readers will also benefit from the inclusion of: An introduction to DNA nano-engineering and DNA-driven nanoparticle assembly Explorations of polysaccharides and glycoproteins, engineered biopolymers, and engineered hydrogels Discussions of macromolecular assemblies, including liquid membranes and small molecule inhibitors for amyloid aggregation A treatment of inorganic nanomaterials as promoters and inhibitors of amyloid fibril formation An examination of a wide variety of natural and artificial polymers Perfect for materials scientists, biochemists, polymer chemists, and protein chemists, Biological Soft Matter: Fundamentals, Properties and Applications will also earn a place in the libraries of biophysicists and physical chemists seeking a one-stop reference summarizing the rapidly evolving topic of biological soft matter.

Download or read book The Giant Vesicle Book written by Rumiana Dimova and published by CRC Press. This book was released on 2019-11-19 with total page 1144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference

Download or read book Physics of Biological Membranes written by Patricia Bassereau and published by Springer. This book was released on 2018-12-30 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike.

Download or read book The Bionano Interactions and Targeting of Nanoparticles written by and published by . This book was released on 2014 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Expansion Microscopy for Cell Biology written by and published by Academic Press. This book was released on 2021-01-19 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Represents the latest release in the Methods in Cell Biology series - Includes the latest information on Expansion Microscopy for Cell Biology

Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction

Download or read book Nanocarrier Technologies written by M. Reza Mozafari and published by Springer Science & Business Media. This book was released on 2006-09-24 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed as an advanced survey of the field, this book describes the key research parameters of nanocarrier technologies. It is the first book with this topic. It comprises a collection of scientific articles from top research people in the field and provides an up-to-date source containing recent citation and bibliography. The book is an indispensable source of information for new researchers and scientists.

Download or read book Intracellular Delivery written by Aleš Prokop and published by Springer Science & Business Media. This book was released on 2011-05-26 with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.