Download or read book In Vitro Mutagenesis written by Andrew Reeves and published by Humana. This book was released on 2016-10-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.
Download or read book In Vitro Mutagenesis Protocols written by Jeff Braman and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.
Download or read book Protocols for Micropropagation of Woody Trees and Fruits written by S.Mohan Jain and published by Springer Science & Business Media. This book was released on 2007-09-18 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.
Download or read book In Vitro Mutagenesis Methods and Protocols written by Andrew Reeves and published by Methods in Molecular Biology. This book was released on 2018-10-20 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.
Download or read book In Vitro Mutagenesis written by and published by . This book was released on 1991 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Mutagenesis and Crop Improvement written by Nitish Kumar and published by CRC Press. This book was released on 2024-02-23 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crop improvement using classically induced mutagenesis is now well standardized. Many new promising varieties of different crops have been successfully developed worldwide using both physical and chemical mutagens. Voluminous literature is now available on basic and applied aspects of mutagenesis. The mutation technique has been refined and holds the promise of generating much wider and more desirable variability than classical breeding. Recent advances in technology combined with classical mutation breeding offer new and exciting challenges for the development of new varieties. A global inventory of induced mutagenesis activities for crop improvement is requried. This book covers both basic and applied aspects of mutation and its impact on various crops: it is extremely well prepared and contains a huge volume of information accumulated using classically induced mutagenesis on different crops in different countries. Three key features: Describes the importance of induced mutation in crop plant research and its application to production Highlights new advances in the understanding of plant mutagenesis in crop improvement Contains contributions from major leaders in the field of plant mutation research This volume brings together all the important and relevant literature in the field. It provides a complete account of the mutation breeding of crops, presenting conclusions about the value of the method, its possibilities, limitations, and shortcomings, and the possible difficulties of further application in various crops. The initial chapters deal with the interactions between mutagenic treatment and plant material, such as aspects of mutagenic treatment, postirradiation behavior of shoot apices, and adventitious bud techniques. All available literature is then discussed crop by crop and critically evaluated. This will serve as an extremely comprehensive guide for researchers, teachers, students, and individuals who are interested in using induced mutagenesis as a tool for crop improvement.
Download or read book In Vitro Mutagenesis Protocols written by Michael K. Trower and published by Springer Science & Business Media. This book was released on 1996 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: In In Vitro Mutagenesis Protocols leading experts from industrial and academic laboratories describe easily reproducible procedures for site-directed and random mutagenesis. Site-directed protocols include those based on strand-selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/ strepavidin system. Many techniques can be used with virtually any double-stranded DNA plasmid. The random mutagenesis protocols include methods based on PCR, degenerate oligonucleotides, cassette mutagenesis, nested deletion mutagenesis, and a specialized E. coli mutator strain. These invaluable protocols facilitate the study of gene regulation and structure/function relationships in proteins and permit modification of DNA sequences for purposes such as vector construction.
Download or read book Microbial genetics applied to biotechnology written by Venetia A. Saunders and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes techniques of microbial genetics and how they may be applied to biotechnology. The text is concerned largely with the application of these techniques to microbial technology. We have therefore utilised illustrative material that is given in our own courses in applied micro biology. The book assumes in the reader a basic knowledge of microbial will prove useful to under genetics and industrial microbiology. We hope it graduates, postgraduates and others taking courses in applied micro biology. We would like to thank various colleagues, including John Carter, Julian Davies, Gordon Dougan, David Hopwood, Gwyn Humphreys, Alan McCarthy, David O'Connor, Tony Hart, Steve Oliver, Roger Pickup, Hilary Richards, Bob Rowlands, David Sherratt, Peter Strike, Richard Sykes and Liz Wellington, all of whom provided information at various stages during the writing of this book. Many thanks are also due to Linda Marsh for patiently typing the many drafts of the manuscript. 1 Introduction Natural genetic variation has always been exploited by man to improve the properties of microbial strains. Spontaneous mutations that arise in micro bial populations and that have properties advantageous to man have been gradually selected over centuries of use. However, it is only since the development of modem genetic techniques that more rational approaches have been possible. Such newer technologies have permitted the tailoring of microorganisms, plant or animal cells to manufacture specific products of commercial or social benefit and to manage the environment.
Download or read book Mutagenicity Assays and Applications written by Ashutosh Kumar and published by Academic Press. This book was released on 2017-09-26 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mutagenicity: Assays and Applications presents an extensive examination of the detection, assessment and future of mutagenicity, particularly as it concerns human health and the environment. Chapters focused on specific types of mutagens or testing methods for their detection collectively explore the current state of human and environmental mutagenesis, future perspectives and regulatory needs. The test procedures for measuring mutagenicity, their advantages and limitations are described with practical and procedural detail, along with their presentation and data processing aspects. It is an essential reference covering the breadth and depth of the field of mutagenicity studies and regulation. By providing both important introductory material and practical assays and applications, this book is useful to graduate students, academic and industry researchers and regulators at various stages of their careers, leading to improved risk assessment and regulation. - Presents an up-to-date and in-depth review of the current state of mutagenesis research - Draws upon the combined experience and expertise of an international group of highly respected editors and chapter authors - Provides an introduction to the concept of mutagenesis with particular consideration given to novel chemicals and materials
Download or read book Abstracts of Papers Presented at the Meeting on in Vitro Mutagenesis written by Robert F. Schleif and published by . This book was released on 1982 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vitro Mutagenesis Protocols written by Jeff Braman and published by Humana Press. This book was released on 2011-08-24 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
Download or read book Environmental Epigenetics written by L. Joseph Su and published by Springer. This book was released on 2015-05-18 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the toxicological and health implications of environmental epigenetics and provides knowledge through an interdisciplinary approach. Included in this volume are chapters outlining various environmental risk factors such as phthalates and dietary components, life states such as pregnancy and ageing, hormonal and metabolic considerations and specific disease risks such as cancer cardiovascular diseases and other non-communicable diseases. Environmental Epigenetics imparts integrative knowledge of the science of epigenetics and the issues raised in environmental epidemiology. This book is intended to serve both as a reference compendium on environmental epigenetics for scientists in academia, industry and laboratories and as a textbook for graduate level environmental health courses. Environmental Epigenetics imparts integrative knowledge of the science of epigenetics and the issues raised in environmental epidemiology. This book is intended to serve both as a reference compendium on environmental epigenetics for scientists in academia, industry and laboratories and as a textbook for graduate level environmental health courses.
Download or read book Procedures and Techniques of in Vitro Mutagenesis written by Cindy A. Rogers and published by . This book was released on 1987 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biotechnologies for Plant Mutation Breeding written by Joanna Jankowicz-Cieslak and published by Springer. This book was released on 2016-12-08 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Download or read book Manual on MUTATION BREEDING THIRD EDITION written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2018-10-09 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
Download or read book Biotechnologies and Genetics in Plant Mutation Breeding written by Tariq Ahmad Bhat and published by CRC Press. This book was released on 2023-06-30 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: "An indispensable source for researchers, teachers, and graduate and postgraduate students interested in mutation breeding and genetic engineering. It introduces readers to contemporary knowledge and state-of-the-art technologies in the field of mutation breeding, including fundamental mechanisms and applications. . . . It will provide new directions, and avenues for enhancement of food security and food quality by using the latest techniques for the 'mutation as breeding' approach." - From Prof. Jameel M. Al-Khayri, King Faisal University, Saudi Arabia This comprehensive three-volume set book aims to help combat the challenge of providing enough food for the world by the use of advanced genetic processes to improve crop production, both in quantity and quality. Volume 1: Mutagenesis and Crop Improvement discusses mutagenesis, cytotoxicity, and crop improvement, covering the processes, mutagenic effectiveness, and mechanisms. The volume emphasizes the improvement of agronomic characteristics by manipulating the genotype of plant species, resulting in increased productivity. Volume 2: Revolutionizing Plant Biology covers the use of mutagenesis and biotechnology to explore the variability of mutant genes for crop improvement. The chapters deal with in-vitro mutagenesis to exploit the somaclonal variations induced in cell culture and highlight the importance of in-vitro mutagenesis in inducing salt resistance, heat resistance, and drought resistance. Volume 3: Mechanisms for Genetic Manipulation of Plants and Plant Mutants reviews the genetic engineering techniques used to mutate genes and to incorporate them into different plant species of cereals, pulses, vegetables, and fruits. Also discussed are the principles of genetic engineering by which desired genes can be transferred from plants to animals to microorganisms and vice versa.
Download or read book In Vitro Mutagenesis of Selected Regions of SV40 DNA written by Patrick Hearing and published by . This book was released on 1980 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
