Download or read book IN VITRO IMAGING USING STABLE WATER DISPERSIBLE LUMINESCENT SI QUANTUM DOTS CAPPED WITH VARIOUS CELLULAR TARGETING AGENTS written by Chen-An Tien and published by . This book was released on 2010 with total page 60 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents research on the synthesis, functionalization, and biological applications of silicon nanocrystals, also called silicon quantum dots (Si QDs). Over the past 20 years, semiconductor nanocrystals (also known as quantum dots or QDs) have been investigated for applications ranging from electronic materials science to biological imaging. The outstanding optical properties of QDs, which include stable luminescence, tunable luminescence color, modifiable surface characteristics, and the potential to interface with biologically relevant molecules, have made QDs a good replacement for organic dyes in many applications. Silicon nanocrystals show many of the same useful optical properties but lower toxicity than cadmium based quantum dots for biological applications.^In this thesis, I focus on the biological applications of silicon nanocrystals in fluorescent biosensing and cellular labeling applications. Conventional quantum dots have great potential in cancer-related imaging and diagnostic applications; however, these applications are limited by concerns about the inherent toxicity of their core materials (e.g. cadmium, lead). Virtually all imaging applications require conjugation of the imaging agent to a biologically active molecule to achieve selective uptake or binding. Here, we report a study of biocompatible silicon quantum dots covalently attached to biomolecules including lysine, folate, anti-mesothelin, and transferrin. The particles possess desirable physical properties, surface chemistry, and optical properties. Folate- and anti-mesothelin-conjugated silicon quantum dots show selective uptake into Panc-1 cells.^This study contributes to the preclinical evaluation of silicon quantum dots and further demonstrates their potential as an imaging agent for cancer applications. Major bionanotechnology research issues in quantum dot synthesis include the stabilization of their optical properties using various coating and encapsulation strategies, and advancing the rational design of constructs containing them to optimize overall size, surface chemistry, and composition in order to minimize potential toxicity and overcome biological barriers. Conventional cadmium- and lead-based quantum dots are normally coated, because their degradation may result in the release of toxic heavy metal ions. For in vivo use, they must be cleared from the body without degradation. Freestanding silicon quantum dots are expected to biodegrade to non-toxic products (e.g. silicic acid); however they have not been evaluated in biodegradable nanocarriers.^Previous work from our group has encapsulated them with non-toxic, but non-biodegradable phospholipid-polyethylene glycol surfactants. Here, we report the development of chitosan-coated silicon nanoparticles (CSi QDs). Evaluation of the physicochemical and optical properties of the CSi QDs shows that they remain optically active in aqueous media. The chitosan coating renders silicon quantum dots stable in aqueous biological media and useful for biological applications such as cellular imaging with single and two photon excitation. The particles are also degradable when incubated at physiological temperature. These results open the door for a new generation of silicon quantum dots that may have a wide variety of applications derived from the flexibility of chitosan.
Download or read book Semiconductor Quantum Dots And Rods For In Vivo Imaging And Cancer Phototherapy written by Maoquan Chu and published by #N/A. This book was released on 2017-06-19 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer is fast becoming one of the main causes of death worldwide. Unfortunately many cases are diagnosed at an advanced incurable stage, and these lives are usually lost. Early diagnosis and treatment are very important for increasing disease curability. In recent years, novel techniques for cancer diagnosis and therapy have been developed, and nanobiomedicine appears to show the most promising results.The application of nanotechnology to biology and medicine in cancer diagnosis is termed nanobiomedicine. Nanoparticles 1-100 nm in size usually have unique physical and/or chemical properties, and this has attracted great attention in the cancer research. Preparation and biomedical applications of the nanoparticles are key components in nanobiomedicine. Semiconductor nanocrystals, including quantum dots (QDs) and quantum rods (QRs), have been extensively investigated for drug delivery, biomedical imaging and tumor target therapy.In Semiconductor Quantum Dots and Rods for In Vivo Imaging and Cancer Phototherapy, the QD and QR optical properties, sentinel lymph node mapping, in vivo tumor target imaging, self-illuminating QDs for in vivo imaging, in vivo cancer photothermal therapy and photodynamic therapy, QD-graphene nanosheet, and QD-magnetic hybrid nanocomposites for bioimaging and cancer therapy are discussed. This book may interest under- and postgraduate students in the field of bioengineering (especially cancer phototherapy) and medical professions alike.
Download or read book Handbook of Photonics for Biomedical Engineering written by Aaron H.-P. Ho and published by Springer. This book was released on 2016-09-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanophotonics has emerged rapidly into technological mainstream with the advent and maturity of nanotechnology available in photonics and enabled many new exciting applications in the area of biomedical science and engineering that were unimagined even a few years ago with conventional photonic engineering techniques. Handbook of Nanophotonics in Biomedical Engineering is intended to be a reliable resource to a wealth of information on nanophotonics that can inspire readers by detailing emerging and established possibilities of nanophotonics in biomedical science and engineering applications. This comprehensive reference presents not only the basics of nanophotonics but also explores recent experimental and clinical methods used in biomedical and bioengineering research. Each peer-reviewed chapter of this book discusses fundamental aspects and materials/fabrication issues of nanophotonics, as well as applications in interfaces, cell, tissue, animal studies, and clinical engineering. The organization provides quick access to current issues and trends of nanophotonic applications in biomedical engineering. All students and professionals in applied sciences, materials, biomedical engineering, and medical and healthcare industry will find this essential reference book highly useful.
Download or read book Development of Fluorescent Semi conductor Nanocrystal Conjugates for in Vitro and in Vivo Imaging Applications written by Hee-Sun Han (Ph. D.) and published by . This book was released on 2012 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Semiconductor nanocrystals, also known as quantum dots (QDs), are promising imaging probes with characteristic optical properties: tunable bandgap from visible to infrared, narrow and symmetric emission features, broad absorption, high quantum yield (QY), excellent photostability, and a large two-photon absorption cross section. However, unlike other imaging probes, the surface ligands determine the solubility, stability, quantum yield (QY), biocompatibility, and derivatizability of QDs. Therefore, to use QDs for in vitro and in vivo imaging, QD ligands need to be elaborately engineered for each experiment. Single cell labeling in vivo requires extremely strict criteria for the QD conjugates to be used such as minimal nonspecific cell/serum binding, maintenance of high QY in complex in vivo environments, and compact size. The focus of this thesis is the synthesis of high quality QD conjugates that can be used for single molecule imaging in vivo and in vivo imaging studies that demonstrate the broad and powerful applicability of our new methods. We incorporated novel conjugation methods employing highly strained cycloolefins and a serum stable tetrazine derivative into newly developed polymeric imidazole ligands (PILs) to efficiently couple biomolecules on QDs. Unlike traditional conjugation methods, tetrazine-norbornene cycloaddition benefits from the non-interacting properties of the functional groups to the QD surface, and yields the high conjugation efficiencies on QDs. In addition, the rapid kinetics, absence of catalyst, and bio-orthogonality of the cycloaddition allowed us to achieve in situ conjugation of the norbornene-bearing QDs to tetrazine-bearing epidermal growth factor (EGF) proteins on the HeLa cell surface. On the in vivo front, we accomplished single endogenous cell imaging in live mice. The ability to target single cells using multiple biomarkers and track them for the extended periods of time allowed us to study the microenvironment of the endogenous hematopoietic stem cells (HSCs), which was not possible using conventional techniques engaging dye conjugated antibodies. Lastly, a new class of QD ligands containing betaine moieties was developed to reduce the size of QD conjugates, which we expect will be greatly beneficial for in vitro and in vivo targeting in dense environments. We successfully demonstrated functionalization of the sulfonate betaine poly imidazole ligands (SBPILs) with biomolecules, and the biocompatibility of SBPIL QDs both in vitro and in vivo.
Download or read book Application of Quantum Dots in Biology and Medicine written by Puspendu Barik and published by Springer Nature. This book was released on 2022-10-03 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates various applications of quantum dots (QDs) in the biomedical field and future perspectives. It first introduces the synthesis procedures and fundamental properties of QDs. In addition, the optical detection techniques and toxicologic reviews of QDs are presented. A focus of the book is also on the applications of QDs in cancer therapy, drug delivery, bio-sensing, and targeted molecular therapy. This book is exciting and valuable to a wide variety of readership communities (students, early-stage researchers, and scientists) in the various fields of biology and medicine.
Download or read book Investigating the Interaction of Semiconductor Quantum Dots with in Vivo and Cellular Environments to Determine Disposition and Risk written by Hans Christian Fischer and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Magnetic Quantum Dots for Bioimaging written by Amin Reza Rajabzadeh and published by CRC Press. This book was released on 2023-06-05 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioimaging is a sophisticated, non-invasive, and non-destructive technique for the direct visualization of biological processes. Highly luminescent quantum dots combined with magnetic nanoparticles or ions form an exciting class of new materials for bioimaging. These materials can be prepared in cost-effective ways and show unique optical behaviors. Magnetic Quantum Dots for Bioimaging explores leading research in the fabrication, characterization, properties, and application of magnetic quantum dots in bioimaging. Covers synthesis, properties, and bioimaging techniques Discusses modern manufacturing technologies and purification of magnetic quantum dots Explores thoroughly the properties and extent of magnetization to various imaging techniques Describes the biocompatibility, suitability, and toxic effects of magnetic quantum dots Reviews recent innovations, applications, opportunities, and future directions in magnetic quantum dots and their surface-decorated nanomaterials This comprehensive reference offers a road map of the use of these innovative materials for researchers, academics, technologists, and advanced students working in materials engineering and sensor technology.
Download or read book Near Infrared Emitting Nanoparticles for Biomedical Applications written by Antonio Benayas and published by Springer Nature. This book was released on 2020-04-13 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book analyzes and evaluates the growing field of light-emitting nanoprobes as contrast agents for in vivo imaging and sensing. It is a comprehensive resource that critically analyzes the state of the art in an interdisciplinary manner, with a special focus on the shift of emission wavelengths into the near-infrared (NIR) spectral region (ranging from 0.7 to 2 microns), which has greatly contributed to the latest advances in biomedical imaging and sensing. This book discusses merits of different contrast agents at nanoscale, and how their unique chemical and structural properties lead to the emission and interaction of light within the NIR window. Both the NIR-emitting materials and various surface modification strategies governing their interactions with the biological system at the “nano” level are discussed. Furthermore, different experimental techniques and protocols for NIR-light-based in vivo imaging and sensing are addressed to shed light on further understanding of the advantages and limitations of each category of these nanoprobes. Assembles the state of the art heretofore appearing in scientific literature into a comprehensive, multi-perspective guidebook on near infrared-emitting nanomaterials in an assortment of biomedical applications; Explains the physical, chemical, and biological phenomena underlying near infrared-emitting nanomaterials for biomedical applications; Presents conceptual and experimental approaches surrounding a unique spectral range of light emission from nanosized contrast agents, while offering a clear explanation of basic and general phenomena regarding the interaction between light and biological tissues, such as absorption, scattering and autofluorescence.
Download or read book Quantum Dots written by Rakshit Ameta and published by Elsevier. This book was released on 2022-09-20 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantum Dots: Fundamentals, Synthesis and Applications compiles key information, along with practical guidance on quantum dot synthesis and applications. Beginning with an introduction, Part One highlights such foundational knowledge as growth mechanisms, shape and composition, electrochemical properties, and production scale-up for quantum dots. Part Two goes on to provide practical guides to key chemical, physical and biological methods for the synthesis of quantum dots, with Part Three reviewing the application of quantum dots and a range of important use cases, including photocatalysis, energy cells and medical imaging. Drawing on the knowledge of its expert authors, this comprehensive book provides practical guidance for all those who already study, develop or use quantum dots in their work. Presents the foundational information needed to effectively understand and manipulate quantum dot properties Consolidates key methods of quantum dot synthesis in a single volume Reviews both current and future practical applications of quantum dots across a range of important fields
Download or read book Quantum Dots as Fluorescent Probes for in Vitro Cellular Imaging written by Milana Coorg Vasudev and published by . This book was released on 2006 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantum Dots for DNA Biosensing written by Jun-Jie Zhu and published by Springer Science & Business Media. This book was released on 2013-12-04 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a broad introduction to all major aspects of quantum dot properties including fluorescence, electrochemical, photochemical and electroluminescence. Such properties have been produced for applications in biosensing, cell tracking, in vivo animal imaging and so on. It focuses on their special applications in DNA biosensing and provides readers with detailed information on the preparation and functionalization of quantum dots and the fabrication of DNA biosensors, using examples to show how these properties can be used in DNA biosensor design and the advantages of quantum dots in DNA biosensing. Further new emerging quantum dots such as metal nanoclusters and graphene dots and their applications in DNA biosensing have also been included.
Download or read book Luminescent Silicon Quantum Dots as a Base for Multimodal Nanoplatforms written by ChingWen Chang and published by . This book was released on 2011 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first reports of their use in biological contexts, QDs have confirmed their potential in biomedical application. QDs can potentially replace organic dyes in most biological applications because of their superior optical properties and photostability. However, most of the quantum dots studied to date, such as CdSe/ZnS, CdS, CdTe and PbSe, incorporate toxic heavy metal components, which may limit their use for in vivo studies. Comparatively few studies have reported the application of silicon quantum dots (SiQDs) in biology and medicine. Compared to other semiconductor materials, silicon is abundant, economical and inherently non-toxic. Silicon nanoparticles can be made via various chemical and physical routes. Our group uses high-temperature decomposition of silane to prepare silicon nanocrystals with typical sizes of 5 to 7 nm in diameter. As described in previous publications, our group is able to obtain water-dispersable silicon nanoparticles that have size-dependent photoluminescence. In spite of that, there are several challenges that must be overcome to use SiQDs as multimodal probes. In the work reported in this thesis, we have used several methods to demonstrate the potential of SiQDs for biosensing and bioimaging applications, including silicon FRET, paramagnetic SiQDs as MRI contrast agents, and SiQDs conjugates for siRNA delivery.
Download or read book Imaging in Photodynamic Therapy written by Michael R. Hamblin and published by CRC Press. This book was released on 2017-02-03 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the broad field of cellular, molecular, preclinical, and clinical imaging either associated with or combined with photodynamic therapy (PDT). It showcases how this approach is used clinically for cancer, infections, and diseases characterized by unwanted tissue such as atherosclerosis or blindness. Because the photosensitizers are also fluorescent, the book also addresses various imaging systems such as confocal microscopy and small animal imaging systems, and highlights how they have been used to follow and optimize treatment, and to answer important mechanistic questions. Chapters also discuss how imaging has made important contributions to clinical outcomes in skin, bladder, and brain cancers, as well as in the development of theranostic agents for detection and treatment of disease. This book provides a resource for physicians and research scientists in cell biology, microscopy, optics, molecular imaging, oncology, and drug discovery.
Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Download or read book Ecole Nationale de dessin et de math matiques pour l application des Beaux Arts l Industrie written by and published by . This book was released on 1875 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Water soluble Luminescent Quantum Dots and Biomolecular Conjugates Thereof and Related Compositions and Method of Use written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The present invention provides a water-soluble luminescent quantum dot, a biomolecular conjugate thereof and a composition comprising such a quantum dot or conjugate. Additionally, the present invention provides a method of obtaining a luminescent quantum dot, a method of making a biomolecular conjugate thereof, and methods of using a biomolecular conjugate for ultrasensitive nonisotopic detection in vitro and in vivo.
Download or read book Luminescent Quantum Dots written by Tara L. Prendergast and published by . This book was released on 2002 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
