Download or read book Immunology Methods Manual MHC ligands and peptide binding written by Ivan Lefkovits and published by . This book was released on 1997 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book MHC Ligands and Peptide Motifs written by Hans-Georg Rammensee and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is centered on a comprehensive list of MHC peptide motifs and ligands as known to date, together with selected T cell epitopes, arranged in an easy-to-read fashion. This information is put into context by chapters on MHC gene organization, MHC structure, T cell epitope prediction, antigen processing and T cell responses. In addition, the book provides a great deal of complementary information: amino acid sequences of MHC class I alpha1 and alpha2 domains and of class II alpha1 and beta1 domains, the established or predicted composition and specificity of MHC pockets, notes on MHC nomenclature including old assignments and reference to useful internet addresses. A handy reference manual that should be helpful for all those dealing with MHC-associated peptides.
Download or read book Immunology Methods Manual Four Volume Set written by and published by Academic Press. This book was released on 1996-12-05 with total page 2495 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Immunology Methods Manual is a four-volume set comprising a comprehensive sourcebook of immunological methods and techniques. Unique in its project-oriented, rather than protocol-oriented approach, the emphasis is placed on describing the environment in which each method was developed and is used. The manual contains both advanced methods and descriptions and basic protocols. It is essential not only for those who study the immune system of man or mouse, but also those applying methods to other species. This new compilation of methods answers the needs of immunologists; molecular, developmental, and cell biologists; students and advanced researchers, and workers in clinical and diagnostic laboratories. Protocols are provided to guide day-to-day laboratory work as well as the development of long-term strategies. Vital contact information also encourages useful dialogue and interaction between authors and method-users. More than 35 section editors and 350 authors have contributed to compile this four-volume practical guide to methods that work! Key Features * Provides essential hands-on methods * Presents protocols with background information * Ideally suited for use in a laboratory * Covers all molecular and cellular techniques Key Features of the CD-ROM * Compatible with Windows, Mac, and UNIX platforms * Features a full range of advanced search capabilities * Includes a personalized note creation feature enabling the user to add bookmarks and "post-its" * Offers full cut, copy, and paste capabilities allowing printing, exporting, and editing * Provides hyperlinks between solutions/reagents and their recipes; figures/tables and their text citations; and reference citations to their bibliographic listings * Contains a Pathway feature which allows recording of content during a particular session or loading a pre-recorded tutorial * Includes the animated film "The Immune System" by André Traunecker and Stefan Meyer
Download or read book Antigen Processing and Presentation Protocols written by Joyce C. Solheim and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.
Download or read book Immunological Recognition of Peptides in Medicine and Biology written by N. D. Zegers and published by CRC Press. This book was released on 1995-07-19 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunological Recognition of Peptides in Medicine and Biology gives a state-of-the-art overview on the use of peptides and peptide-ligand interactions, and the critical role they play in recognition patterns for the regulation of various biological functions. A wide range of applications are discussed, including some experimental preclinical ones such as epitope mapping, peptide libraries, and production of amino acid-specific antibodies and their therapeutic use in oncology and infectious disease vaccines. Each chapter also includes step-by-step protocols to aid in actual experiments. Several alternative techniques and strategies are discussed by different authors offering the reader an opportunity to select the most favorable application for a specific biological problem.
Download or read book Immunology Methods Manual Immunohistological tools written by Ivan Lefkovits and published by . This book was released on 1997 with total page 738 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book MHC Molecules Expression Assembly and Function written by Robert G. Urban and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3 nant expression systems have been used to make MHC molecules con taining a single peptide of interest. To date, fifteen single peptide class I structures (incorporating three different HLA and two different H-2 allotypes/isotypes) and four additional class II structures (two single peptide complexes and two superantigen complexes) have been reported. These advances have enabled us to study the atomic detail of antigen presentation and the general mechanisms behind peptide binding, and begin to construct models of T cell recognition. Another area of research which has exploded over the past five years has been the identification of MHC-associated peptides. There are several methods one can use to determine the sequence identity of MHC restricted peptides. Historically, the most successful technique, albeit crude and encumbered with serious limitations, has been the use of overlapping synthetic peptides and T cell clones. Unfortunately, this method absolutely requires: (i) knowledge of the target antigen; (ii) availability of T cell clones; and (iii) a relatively short overall length for the target source protein, such that a set of overlapping pep tides can be affordably synthesized. Briefly, the entire sequence of the tar get protein is chemically synthesized using overlapping peptides which are then screened for biological activity using standard T cell presen tation assays. Despite its limitations, this method was used to identify the first immunodominant epitopes reported in the literature and con tinues to be used successfully today.
Download or read book Immunology Methods Manual Expression of recombinant proteins written by Ivan Lefkovits and published by . This book was released on 1997 with total page 686 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Antigen Processing and Presentation Protocols written by Joyce C. Solheim and published by Humana. This book was released on 2000-09-15 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.
Download or read book Immunology Methods Manual Immunodiagnosis of human B cell malignancies written by Ivan Lefkovits and published by . This book was released on 1997 with total page 804 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of Hematopoiesis 6 written by Nader G. Abraham and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Molecular Biology of Hematopoiesis is dedicated to many inter national scientists and clinicians for their contribution to the field of Hematology/ Oncology presented at the 11th International Symposium on Molecular Biology of Hematopoiesis, which was held in Bormio, Italy, June 25-29, 1998. The continuous support of the Presidents of the meeting, Professor F. Takaku, President of Jichi University, and E. D. Thomas, Nobel Laureate, was greatly acknowledged, especially Professor Takaku, for his vision and support for development of gene therapy in Japan. New information on BMT for autoimmune disease and organ transplantation was presented at the symposium and is published in this volume. Several new findings on gene therapy/transfer into HSC were presented by E. F. Vanin and A. Nienhuis, K. Humphries, 1. A. Nolta, H. E. Heslop, and M. K. Brenner. Professors S. Asano and K. Tani presented new studies on gene transfer into primates. Among the highlights were the new papers on gene transfer presented by G. Wage maker, N. G. Abraham, and M. Onoderea from R. M. BJaese's group. The use of BMT for organ transplant and autoim mune disease was updated and a representative paper is presented in this volume.
Download or read book MHC written by Dr. Nelson Fernandez and published by IRL Press. This book was released on 1997 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: The major histocompatibility complex is the subject of much research in the immunology field. There is a great deal of interest in the structure of MHC proteins and in their function as antigen presenting molecules. An increasing number of immunology laboratories are preparing to investigatebiochemical and genetic techniques in order to study these molecules. The identification of the peptide transporter genes and the elution of peptides from MHC molecules demonstrate how rapidly our understanding of the structure and function of the MHC locus has advanced. MCH1: A Practical Approachbrings together the technologies which facilitate the advances that are being made. It has detailed explanations of processes such as peptide translocation into the ER, the application of mass spectrometry to the analysis of peptides bound to MHC, and signal detection via MHC class II moleculeswhich can lead to either activation or programmed cell death. This book is a comprehensive and thorough guide to the fast growing and fascinating field of MHC research. Graduates, post doctorial researchers, academic staff and clinicians in the immunology field will find this book an invaluableaid.
Download or read book In Silico Immunology written by Darren D.R. Flower and published by Springer Science & Business Media. This book was released on 2007-04-16 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book outlines three emergent disciplines, which are now poised to engineer a paradigm shift from hypothesis- to data-driven research: theoretical immunology, immunoinformatics, and Artificial Immune Systems. It details how these disciplines will enable new understanding to emerge from the analysis of complex datasets. Coverage shows how these three are set to transform immunological science and the future of health care.
Download or read book Molecular Mechanism of Peptide Selection by MHC Molecules written by Hirstova Rakina Yaneva and published by . This book was released on 2009 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major histocompatibility complex (MHC) class I and II proteins are intracellular receptors that bind peptides derived from endogenous and exogenous proteins, resp., and display them on the cell surface for surveillance by T cells of the immune system. The presented peptides help the T cells to discriminate between self and non-self and to eliminate the pathogen infected or abnormal cells. After their synthesis, both class I and II molecules first bind suboptimal low-affinity ligand peptides and only later exchange them for high-affinity ones. For this exchange process, most class I and II molecules need the assistance of specific helper chaperones (tapasin and HLA-DM, resp.), the exact role of which is not well understood. In my thesis work, I have studied the molecular mechanism of peptide binding to MHC molecules with biochemical and computational methods, with a special emphasis on the mechanism of action of the helper chaperones. A comprehensive understanding of this process promises important clinical applications, for example in the development of new vaccines against viruses and in the suppression of autoimmune disorders
Download or read book Manual of Molecular and Clinical Lab Immunology written by Barbara Detrick and published by American Society for Microbiology Press. This book was released on 2006-03-10 with total page 3351 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces new material that reflects the significant advances and developments in the field of clinical laboratory immunology. • Provides a comprehensive and practical approach to the procedures underlying clinical immunology testing. • Emphasizes molecular techniques used in the field of laboratory immunology. • Updates existing chapters and adds significant new material detailing molecular techniques used in the field. • Presents guidelines for selecting the best procedures for specific situations and discusses alternative procedures. • Covers aspects of immunology related disciplines such as allergy, autoimmune diseases, cancers, and transplantation immunology.
Download or read book Immunological Bioinformatics written by Ole Lund and published by MIT Press. This book was released on 2024-02-06 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using bioinformatics methods to generate a systems-level view of the immune system; description of the main biological concepts and the new data-driven algorithms. Despite the fact that advanced bioinformatics methodologies have not been used as extensively in immunology as in other subdisciplines within biology, research in immunological bioinformatics has already developed models of components of the immune system that can be combined and that may help develop therapies, vaccines, and diagnostic tools for such diseases as AIDS, malaria, and cancer. In a broader perspective, specialized bioinformatics methods in immunology make possible for the first time a systems-level understanding of the immune system. The traditional approaches to immunology are reductionist, avoiding complexity but providing detailed knowledge of a single event, cell, or molecular entity. Today, a variety of experimental bioinformatics techniques connected to the sequencing of the human genome provides a sound scientific basis for a comprehensive description of the complex immunological processes. This book offers a description of bioinformatics techniques as they are applied to immunology, including a succinct account of the main biological concepts for students and researchers with backgrounds in mathematics, statistics, and computer science as well as explanations of the new data-driven algorithms in the context of biological data that will be useful for immunologists, biologists, and biochemists working on vaccine design. In each chapter the authors show interesting biological insights gained from the bioinformatics approach. The book concludes by explaining how all the methods presented in the book can be integrated to identify immunogenic regions in microorganisms and host genomes.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
