Download or read book The Immune Synapse written by Cosima T. Baldari and published by Humana. This book was released on 2024-04-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, The Immune Synapse: Methods and Protocols, Second Edition serves as an ideal practical guide for researchers working in this dynamic field. Chapters 5, 11,18, 27, 30, and 32 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Immunological Synapse written by Takashi Saito and published by Springer Science & Business Media. This book was released on 2009-12-03 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proper physiological functioning of most eukaryotic cells requires their assembly into multi-cellular tissues that form organized organ systems. Cells of the immune system develop in bone marrow and lymphoid organs, but as the cells mature they leave these organs and circulate as single cells. Antigen receptors (TCRs) of T cells search for membrane MHC proteins that are bound to peptides derived from infectious pathogens or cellular transformations. The detection of such speci?c peptide–MHC antigens initiates T cell activation, adhesion, and immune-effectors functions. Studies of normal and transformed T cell lines and of T cells from transgenic mice led to comprehensive understanding of the mole- lar basis of antigen-receptor recognition and signaling. In spite of these remarkable genetic and biochemical advances, other key physiological mechanisms that par- cipate in sensing and decoding the immune context to induce the appropriate cellular immune responses remain unresolved. TCR recognition is tightly regulated to trigger sensitive but balanced T cell responses that result in the effective elimination of the pathogens while minimizing collateral damage to the host. The sensitivity of TCR recognition has to be properly tempered to prevent unintended activation by self-peptide–MHC complexes that cause autoimmune diseases. It is likely that once the TCR is engaged by a peptide– MHC and TCR signaling begins, additional regulatory mechanisms, involving other receptors, would increase the ?delity of the response.
Download or read book Structural Biology in Immunology written by Chaim Putterman and published by Academic Press. This book was released on 2018-05-14 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structural Biology in Immunology, Structure/Function of Novel Molecules of Immunologic Importance delivers important information on the structure and functional relationships in novel molecules of immunologic interest. Due to an increasingly sophisticated understanding of the immune system, the approach to the treatment of many immune-mediated diseases, including multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, and inflammatory bowel disease has been dramatically altered. Furthermore, there is an increasing awareness of the critical role of the immune system in cancer biology. The improved central structure function relationships presented in this book will further enhance our ability to understand what defects in normal individuals can lead to disease. - Describes novel/recently discovered immunomodulatory proteins, including antibodies and co-stimulatory or co-inhibitory molecules - Emphasizes new biologic and small molecule drug design through the exploration of structure-function relationship - Features a collaborative editorial effort, involving clinical immunologists and structural biologists - Provides useful and practical insights on developing the necessary links between basic science and clinical therapy in immunology - Gives interested parties a bridge to learn about computer modeling and structure based design principles
Download or read book Signaling Mechanisms Regulating T Cell Diversity and Function written by Jonathan Soboloff and published by CRC Press. This book was released on 2017-03-27 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.
Download or read book Molecular Dynamics at the Immunological Synapse written by Pedro Roda-Navarro and published by Frontiers Media SA. This book was released on 2017-03-21 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: The immunological synapse (IS) is a specialised cell-cell adhesion that mediates antigen acquisition and regulates the activation of lymphocytes. Initial studies of the IS showed a structure composed of stable supra-molecular activation clusters (SMAC) organised during the interaction of helper T lymphocytes with B lymphocytes, working as antigen presenting cells. A central SMAC of coalesced T cell receptors (TCRs) and a peripheral SMAC for cell-cell adhesion were observed. IS with similar structure was later described during antigen acquisition by B cells and during the interaction of NK cells with target and healthy cells. More recent research developed with microscopy systems that improve the spatial and temporal resolution has showed the complex molecular dynamics at the IS that governs lymphocyte activation. Currently, the IS is seen as a three-dimensional structure where signalling networks for lymphocyte activation and endosomal and cytoskeleton machinery are polarised. A view has emerged in which dynamic microclusters of signalling complexes are composed of molecular components attached to the plasma membrane and other components conveyed on sub-synaptic vesicles transported to the membrane by cytoskeletal fibers and motor proteins. Much information is nonetheless missing about how the dynamics of the endosomal compartment, the cytoskeleton, and signalling complexes are reciprocally regulated to achieve the function of lymphocytes. Experimental evidence also suggests that the environment surrounding lymphocytes exposed to different antigenic challenge regulates IS assembly and functional output, making an even more complex scenario still far from being completely understood. Also, although some signalling molecular components for lymphocyte activation have been identified and thoroughly studied, the function of other molecules has not been yet uncovered or deeply characterised. This research topic aims to provide the reader with the latest information about the molecular dynamics governing lymphocyte activation. These molecular dynamics dictate cell decisions. Thus, we expect that understanding them will provide new avenues for cell manipulation in therapies to treat different immune-related pathologies.
Download or read book The Immunological Synapse Part B written by and published by Elsevier. This book was released on 2023-07-27 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay. - Covers various methods related to the study of the immunological synapse - Includes detailed, point-by-point, methods as well as various important notes - Provides the authority and expertise from an international board of leading scientists
Download or read book The Immunological Synapse Part A written by and published by Elsevier. This book was released on 2023-01-16 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more. Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L. - Covers various methods related to the study of the immunological synapse - Includes detailed, point-by-point, methods as well as various important notes - Provides the authority and expertise from an international board of leading scientists
Download or read book Measuring Immunity written by Michael T. Lotze and published by Elsevier. This book was released on 2011-05-04 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most of the diseases of modern mankind involve either acute or chronic inflammation. Measuring Immunity integrates the current information available on biomarkers and surrogate assays into a single handbook. It highlights the principles behind various applications, gives a brief summary on how they are conducted and provides detailed and critical analyses of murine models of immunity, clinical trials, and tests to predict utility and benefit. Measuring Immunity is indispensable for scientists and clinicians interested in the clinical applications of modern immunobiology.* Defines which assays of immune function are helpful in the assessment of clinical disorders involving inflammation and immunity* Assesses the dynamics of cellular and soluble factors in the peripheral blood using modern techniques * Includes basic science foundations as well as the approaches currently applied
Download or read book The Immune Synapse as a Novel Target for Therapy written by Luis Graca and published by Springer Science & Business Media. This book was released on 2007-12-18 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume gives an overview on the progress in immune synapse research, from basic science to clinical trials, and the major mechanisms involved. It discusses how interfering with T cell activation may lead to immune tolerance, immune modulation, and the recruitment of regulatory T cells; the role of monoclonal antibodies in tolerance induction; and mechanisms maintaining dominant tolerance.
Download or read book The Golgi Apparatus and Centriole written by Malgorzata Kloc and published by Springer. This book was released on 2020-09-04 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume takes a closer look how the cell organelles Golgi apparatus (also known as the Golgi complex or Golgi body), and centriole are structurally and functionally intertwined. Initially, it was believed that the role of Golgi complex is limited to the packaging and preparation for secretion of various cellular proteins, while the centriole participates in cell division and cilia formation. However, since their discovery nearly 200 years ago, it became clear that these two organelles are interacting, and that their functions are much more complex and far reaching than previously thought. Recent findings indicate that the Golgi–Centriole relationship may be important for directional protein transport, cell polarization and cell cycle progression. Current studies indicate that Golgi and centriole also participate in development and act as cellular and immunological sensors, and that their abnormalities lead to cell and developmental abnormalities, Alzheimer, cancer, various lipid disorders and neurological and immunological diseases in humans. This volume combines the latest information on the structure, molecular composition, and roles of Golgi and centriole in various cellular functions and diseases. The better understanding of the Golgi–centriole interactions may lead to the development of novel therapies for the treatment of various diseases, including cancer.
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book IMMUNOLOGY written by I. KANNAN and published by MJP Publisher. This book was released on 2019-06-11 with total page 575 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunology as a scientific discipline deals with the study of the immune system. This book on Immunology provides an in-depth coverage of the immune system and the various principles lying behind its effective functioning. The systematic organization of chapters with the inclusion of recent advances in the field of immunology make this a treatise. The topics are dealt in simple language with numerous illustrations to provide ease of learning. Important additional information relating to this field is provided as boxed items.
Download or read book The Immune Synapse written by Cosima T. Baldari and published by Springer Nature. This book was released on 2023-04-27 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, The Immune Synapse: Methods and Protocols, Second Edition serves as an ideal practical guide for researchers working in this dynamic field. Chapters 5, 11, 18, 27, 30, and 32 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book ImmunoPhysics and ImmunoEngineering written by Jorge Bernardino De La Serna and published by Frontiers Media SA. This book was released on 2020-03-30 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: ImmunoPhysics (ImmPhys) and ImmunoEngineering (ImmPhysEng), are two cross-disciplinary fields. ImmPhysEng aims to unravel quantitatively the immune-system function and regulation in health and disease. Whereas ImmPhys study and assess the physical basis of the immune response, ImmEng pursues its control and prediction. Ultimately, the overarching goal of these disciplines is to facilitate the development of therapeutic interventions to more precisely modulate and control the compromised immune response during diseases. Lately, these disciplines are becoming more popular and as such, the number of publications applying physical or engineering tools to understand the immune response is increasing. Nevertheless, there is still no scientific forum compiling the ImmPhysEng research breakthroughs. Possibly the biggest burden is to stimulate a fluent communication and syntony between a physicist or engineer and an immunologist.
Download or read book T Cell Motility written by Navin Kumar Verma and published by Humana. This book was released on 2019-01-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.
Download or read book Current Topics in Membranes written by and published by Academic Press. This book was released on 1992-01-08 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
