Download or read book Immunochemical Methods in Cell and Molecular Biology written by and published by Academic Press. This book was released on 1987 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents up-to-date immunochemical techniques for isolating and analyzing a wide variety of biological products. It collates existing information in one source, presenting important basic and applied advances and provides a stimulating forum for the discussion of new ideas and observations on genetically manipulated microbial and viral agents.
Download or read book Immunochemical Methods in Cell and Molecular Biology written by Roland John Mayer and published by . This book was released on 1987 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunochemical Protocols written by John Pound and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Download or read book Immunochemical Methods in the Biological Sciences written by R. J. Mayer and published by . This book was released on 1980 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.
Download or read book Immunochemical Protocols written by Robert Burns and published by Humana. This book was released on 2004-12-13 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.
Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.
Download or read book Immunochemical Protocols Methods in Molecular Biology written by John Pound and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities."
Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by . This book was released on 1998-07-22 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory Methods in Immunology written by Heddy Zola and published by CRC Press. This book was released on 1990-05-23 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
Download or read book Immunocytochemical Methods and Protocols written by Constance Oliver and published by Humana Press. This book was released on 2012-04-06 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Download or read book Immunochemical Techniques for the Identification and Estimation of Macromolecules written by Jorgen Clausen and published by . This book was released on 1988 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunochemical Techniques for the Identification and Estimation of Macromolecules written by Jørgen Clausen and published by . This book was released on 1981 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunochemistry in Practice written by Alan P Johnstone and published by Wiley-Blackwell. This book was released on 1996-06-03 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical book provides scientists in all disciplines with clear descriptions of the plethora of immunochemical techniques available for detecting, isolating and characterising biological molecules. The revolution in molecular biology and biotechnology in the past decade has meant that immunochemistry has become an integral part of the experimental armoury of scientists from a variety of backgrounds. The heart of this technology is the remarkable specificity that antibodies display toward their epitopes. The specific recognition offered by immunological molecules has resulted in their uptake for a wide range of purposes. This book explains how to produce and purify antibodies and how to tag them for later visualisation.
Download or read book Immunohistochemistry and Immunocytochemistry written by Luis Del Valle and published by . This book was released on 2022 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.
Download or read book Immunochemical Methods in Cell and Molecular Biology written by R. J. Mayer and published by . This book was released on 1987 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Physical and Chemical Methods written by Curtis A. Williams and published by Academic Press. This book was released on 2014-05-12 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.
