Download or read book Immobilized Reagents for Protein Modification written by Catherine D. Lewis and published by . This book was released on 1978 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2014-07-22 with total page 678 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the

Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers. As a result, little emphasis is given to the individual consideration of the functional consequence of chemical modification.

Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2018-01-18 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers.

Download or read book CHEMICAL REAGENTS FOR PROTEIN MODIFICATION written by Roger L. Lundblad and published by Springer. This book was released on 1984-04-18 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.

Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

Download or read book Reagents for Protein Analysis and Modification written by Troy A. Rhonemus and published by . This book was released on 1998 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Immobilized Affinity Ligand Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 1992-08-27 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References

Download or read book Reagents for Protein Modification written by Fluka Chemie and published by . This book was released on 1986 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Application of in Vacuo Chemical Modification for Protein Characterization and Enhancement of the Physico chemical Properties of Proteins written by Van Thông Pham and published by . This book was released on 2008 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Split Inteins written by Henning D. Mootz and published by Humana. This book was released on 2016-10-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Protocols explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split-intein based technologies to prepare cyclic peptides and proteins. The next few chapters discuss semisynthetic protein trans-splicing using one synthetic intein piece, synthetic intein-extein pieces used to deliver other cargos for chemical modification both of purified proteins and of proteins in living cells, as well as isotopic labeling of proteins for NMR studies, and a discussion on how protein block copolymers can be generated by protein trans-splicing to form protein hydrogels. The last few chapters deal with intein applications in transgenic plants and conditional inteins that can be regulated in artificial ways by small molecules or light, a cassette-based approach to quickly test many intein insertion positions, and a computational approach to predict new intein split sites (the approach also works for other proteins). Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Split Inteins: Methods and Protocols is a valuable resource that will provide guidance toward possibilities of split intein applications, explore proven and detailed protocols adaptable to various research projects, and inspire new method developments.

Download or read book Development of Some New Protein modification Reagents written by Marlene Amjad Bunni and published by . This book was released on 1984 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chemical Modification of Proteins written by Gary E. Means and published by . This book was released on 1971 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of Novel Maleimide Reagents for Protein Modification written by Cristina Marculescu Panea and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of Novel Maleimide Reagents for Protein Modification written by and published by . This book was released on 2014 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioanalytical Chemistry written by Susan R. Mikkelsen and published by John Wiley & Sons. This book was released on 2016-03-07 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry Provides an all in one approach for both beginners and experts, from a broad range of backgrounds, covering introductions, theory, advanced concepts and diverse applications for each method Each chapter progresses from basic concepts to applications involving real samples Includes three new chapters on Biomimetic Materials, Lab-on-Chip, and Analytical Methods Contains end-of-chapter problems and an appendix with selected answers