Download or read book Immobilized pH Gradients Theory and Methodology written by Pier Giorgio Righetti and published by Elsevier. This book was released on 1990-02-07 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Download or read book Immobilized PH Gradients Theory and Methodology written by P. G. Righetti and published by Elsevier. This book was released on 1990 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-05-16 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by R. H. Burdon and published by . This book was released on 1990 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteome Research Two Dimensional Gel Electrophoresis and Identification Methods written by T. Rabilloud and published by Springer Science & Business Media. This book was released on 1999-09-17 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.
Download or read book Western Blotting for the Non Expert written by Biji T. Kurien and published by Springer Nature. This book was released on 2021-07-24 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..
Download or read book 2 D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
Download or read book Handbook of Isoelectric Focusing and Proteomics written by David Garfin and published by Elsevier. This book was released on 2005-09-29 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.* Is the only current book available on the subject * Includes author insights and practical tips* Is an ideal single source for students and researchers working in proteomics
Download or read book Bioanalytical Chemistry written by Susan R. Mikkelsen and published by John Wiley & Sons. This book was released on 2004-02-19 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioanalytical Chemistry provides a thorough introduction for students and practitioners with a broad range of backgrounds from chemistry to medicine. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists. The text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The authors have limited mathematical derivations to those that are essential for an understanding of each method and they include a list of suggested reading for further information. This textbook provides an ideal companion for students, researchers, and industrial scientists working in chemistry, biology, biochemistry, pharmacy, and medicine.
Download or read book Electrophoretic Separation of Proteins written by Biji T. Kurien and published by . This book was released on with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Download or read book Methods in Animal Proteomics written by Philip Whitfield and published by John Wiley & Sons. This book was released on 2011-07-05 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.
Download or read book Electrophoresis 83 written by Hidematsu Hirai and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-07-08 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "Electrophoresis '83".
Download or read book Electrophoresis written by Budin Michov and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-09-07 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists.
Download or read book Scientific Foundations of Biochemistry in Clinical Practice written by David L. Williams and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 807 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Foundations of Biochemistry in Clinical Practice, Second Edition describes the pathological aspects of general metabolic disorders. This book is organized into 10 sections encompassing 45 chapters that discuss the nature of disorders involving disturbance in hydrogen ion concentration and blood gases, as well as the disorders of fluid and electrolyte balance. Some of the topics covered in the book are the chemical analysis of urine; neonatal screening for biochemical disorders; clinical biochemistry of alcohol, in intensive and postoperative care; psychiatric disorders of biochemical origin; abnormalities of the plasma proteins; assessment of gastrointestinal function; and calcium metabolism. Other chapters examine the nature, conditions, and diagnosis of bone disorders. A chapter emphasizes the functions of hypothalamus and pituitary. Another chapter looks into the biochemistry and toxicology of metals. The final chapters are devoted to the classification of connective tissue diseases and to the examination of clinical biochemistry of the central nervous system. The book can provide useful information to doctors, biochemists, students, and researchers.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1099 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1988 with total page 1328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
