Download or read book Identification of Subsurface Microorganisms at Yucca Mountain Quarterly Report July 1 1995 September 30 1995 written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification of Subsurface Microorganisms at Yucca Mountain Second Quarterly Report October 1 1993 December 31 1993 written by and published by . This book was released on 1993 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt: The primary effort of this past quarter was to develop a procedure where accumulated data files could be evaluated to determine the naming consistency and inter-relationships of the various species which have been identified by the Microbial Identification System (MIDI) system. This involved a series of steps, including the clustering of similarly named organisms in a dendrogram format to determine how closely similarly named isolates are related. The experience of other researchers using the MIDI system has shown that clusters which are joined at a Euclidian distance of 10 or less belong to the same species. Strains which are very similar cluster at less than 6 Euclidian units and clusters below two units have nearly identical fatty acid patterns. When the dendrograms derived from the springs were scrutinized, some organisms were found which did not match the pattern of their named group. Then a decision was made whether to rename the isolates and exclude them from the group or redefine the group. This decision was assisted by plotting the principal components derived from an analysis of the fatty acid composition of members of the genus. Each species can be examined by the same procedure to determine group homogeneity. In these 2-dimensional plots members of the same species are roughly bounded by a box of 100 squared units while closely related strains are grouped more tightly together. The 2-dimensional plot of isolates of Micrococcus luteus demonstrates the presence of three identifiable sub-species.
Download or read book Identification of Subsurface Microorganisms at Yucca Mountain Fourth Quarterly Report written by and published by . This book was released on 1993 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria isolated from water samples collected in a series of ground water springs have been isolated, enumerated, and identified from twenty six sites. Ten sites were sampled in Death Valley, California and sixteen sites were sampled in Ash Meadows, Nevada. Replicate samples were collected and tested from four locations. All water samples were collected in conjunction with the HRC chemistry group conducting ground water fingerprinting studies. The protocol for collection of samples, as described in the 3rd quarterly report, specified aseptic collection in sterile screw-capped containers and transportation on ice to the HRC microbiology laboratory. All samples were inoculated by spread plating onto R2A (Difco Laboratories, Detroit, MI) bacterial culture medium. the R2A plates were then incubated at 28° for 5--7 days and colonies wee counted with the aid of a grid template and magnifying lens.
Download or read book Identification of Subsurface Microorganisms at Yucca Mountain Third Quarterly Report January 1 1994 March 31 1994 written by and published by . This book was released on 1994 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria isolated from ground water samples taken from 31 springs during 1993 were collected and processed according to procedures described in earlier reports. These procedures required aseptic collection of surface water samples in sterile screw-capped containers, transportation to the HRC microbiology laboratory, and culture by spread plating onto R2A medium. The isolates were further processed for identification using a gas chromatographic analysis of fatty acid methyl esters (FAME) extracted from cell membranes. This work generated a presumptive identification of 113 bacterial species distributed among 45 genera using a database obtained from Microbial ID, Inc., Newark, Delaware (MIDI). A preliminary examination of the FAME data was accomplished using cluster analysis and principal component analysis software obtained from MIDI. Typically, bacterial strains that cluster at less than 10 Euclidian distance units have fatty acid patterns consistent among members of the same species. Thus an organism obtained from one source can be recognized if it is isolated again from the same or any other source. This makes it possible to track the distribution of organisms and monitor environmental conditions or fluid transport mechanisms. Microorganisms are seldom found as monocultures in natural environments. They are more likely to be closely associated with other genera with complementary metabolic requirements. An understanding of the indigenous microorganism population is useful in understanding subtle changes in the environment. However, classification of environmental organisms using traditional methods is not ideal because differentiation of species with small variations or genera with very similar taxonomic characteristics is beyond the capabilities of traditional microbiological methods.
Download or read book Yucca Mountain Project Quarterly Technical Progress Report July 1 1995 September 30 1995 written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Government Reports Announcements Index written by and published by . This book was released on 1996 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Radioactive Waste Management written by and published by . This book was released on 1995 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Energy Research Abstracts written by and published by . This book was released on 1995 with total page 782 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book INIS Atomindex written by and published by . This book was released on 1995 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Global Climate Change Impacts in the United States written by U.S. Global Change Research Program and published by Cambridge University Press. This book was released on 2009-08-24 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizes the science of climate change and impacts on the United States, for the public and policymakers.
Download or read book Hydrology and Geochemistry of Yucca Mountain and Vicinity Southern Nevada and California written by John S. Stuckless and published by Geological Society of America. This book was released on 2012 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulatory and Technical Reports written by U.S. Nuclear Regulatory Commission. Division of Technical Information and Document Control and published by . This book was released on 1980 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes indexes.
Download or read book Process Design Manual written by and published by . This book was released on 1995 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Pollution Abstracts written by and published by . This book was released on 1995 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ecological Regions of North America written by and published by . This book was released on 1997 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume represents a first attempt at holistically classifying and mapping ecological regions across all three countries of the North American continent. A common analytical methodology is used to examine North American ecology at multiple scales, from large continental ecosystems to subdivisions of these that correlate more detailed physical and biological settings with human activities on two levels of successively smaller units. The volume begins with an overview of North America from an ecological perspective, concepts of ecological regionalization. This is followed by descriptions of the 15 broad ecological regions, including information on physical and biological setting and human activities. The final section presents case studies in applications of the ecological characterization methodology to environmental issues. The appendix includes a list of common and scientific names of selected species characteristic of the ecological regions.
Download or read book Technical Bases for Yucca Mountain Standards written by National Research Council and published by National Academies Press. This book was released on 1995-07-28 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: The United States currently has no place to dispose of the high-level radioactive waste resulting from the production of the nuclear weapons and the operation of nuclear electronic power plants. The only option under formal consideration at this time is to place the waste in an underground geologic repository at Yucca Mountain in Nevada. However, there is strong public debate about whether such a repository could protect humans from the radioactive waste that will be dangerous for many thousands of years. This book shows the extent to which our scientific knowledge can guide the federal government in developing a standard to protect the health of the public from wastes in such a repository at Yucca Mountain. The U.S. Environmental Protection Agency is required to use the recommendations presented in this book as it develops its standard.
Download or read book Toxicological Profile for Cresols written by and published by . This book was released on 1992 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
