Download or read book Identification and Functional Characterization of a Long Non coding RNA Associated with Prostate Cancer written by Md Faqrul Hasan and published by . This book was released on 2019 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the most common cancer in men in the western world. Although early stage prostate cancer is treatable late stage, more specifically, metastatic and drug resistant prostate cancers are mostly incurable. The failure of current treatments obligates the research community to explore novel areas in prostate cancer biology and find better therapeutic targets. Emerging evidences show that non-coding RNAs specifically long non-coding RNAs (lncRNAs) play regulatory roles in various cellular processes and are frequently dysregulated in cancer including prostate cancer. These aberrantly expressed lncRNAs mostly with unexplored genetic information may drive cancer progression. Previous studies done in our laboratory showed a tumor suppressor role of a cluster of small non-coding RNAs or microRNA (miRNA) miR-17-92a in PC-3 prostate cancer cells. To learn the underlying mechanism, transcriptome analysis with or without expression of miR-17-92a was conducted in our laboratory. RNA-sequencing data analysis identified reduced expression of a set of lncRNAs and oncogenes, and up regulation of several tumor suppressor genes upon expression of miR-17-92a cluster miRNAs. One of the down regulated intergenic lncRNAs, PAINT (Prostate Cancer Associated Intergenic Non-coding Transcript) (LINC00888), was selected for determining its functional role in prostate cancer. TCGA and GEO profiles analyses revealed up regulation of PAINT in prostate tumors with higher Gleason Scores, in highly aggressive metastatic prostate cancer cell lines, and upon androgen deprivation therapy of prostate cancer cells.

Download or read book Fibrous Proteins Structures and Mechanisms written by David A.D. Parry and published by Springer. This book was released on 2017-01-18 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the readers with an up-to-date review of the design, structure and function of a representative selection of fibrous proteins in both health and disease. The importance of the α-helical coiled coil, a conformational motif based on the heptad repeat in the amino acid sequence of all α-fibrous proteins (and parts of some globular proteins) is underlined by three Chapters devoted to its design, structure, function and topology. Specific proteins covered in the text and which depend on the coiled coil for their structure and function, include the intermediate filament proteins, tropomyosin, myosin, paramyosin, fibrin and members of the spectrin superfamily. Also described are fibrous proteins based on the β-pleated sheet and collagen conformations. Recombinant structural proteins, especially of silk and collagen, are discussed in the context of developing new biomaterials with varied applications. Established researchers and postgraduate students in the fields of protein chemistry, biochemistry and structural biophysics will find Fibrous Proteins: Structures and Mechanisms to be an invaluable collection of topical reviews that describe the basic advances made in the field of fibrous proteins over the past decade. This book, written by recognized authorities in the field, provides a clear account of the current status of fibrous protein research and, in addition, establishes the basis for deciding the most appropriate directions for future activity, including the applications of protein engineering and the commercial exploitation of new biomaterials.

Download or read book Functional Characterization of Two Novel Human Prostate Cancer Metastasis Related Genes written by and published by . This book was released on 2005 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: We propose to identify the functional characterization of two novel cancer-specific, metastasis-related genes whose constitutive expression may be pivotal for prostate cancer progression. Work accomplished was performed based on the proposed statement of work. We have characterized the full-length cDNAs of the Seq1 and Seq2 genes using at least three 5' and '3 rapid amplifications of cDNA ends (RACE) commercial kits (Invitrogen Carlsbad, CA, BD Bioscience (Clontech Inc), and Seegene, Rockville, MD). To optimize the PCR conditions for each kit, we had designed several sets of gene-specific primers (GSP;23-28 nt long) with 50-70% GC and Tm of 55 to 75 degrees C for each gene. We have also designed several sets of nested GSPs to verify our cloned genes. Because of unique secondary structures, high GC content, short SSH sequences, and low levels of expression of these genes in prostate cancer cell lines, we had great deal of difficulty in accomplishing this task in a timely fashion. As such, we devised different strategies for the first-strand synthesis using a modified oligo(dT) primers (5'-CDS primer or 3'-CDS primer), and Smart oligo II primer under various conditions. The full-length cDNA sequences were subcloned into mammalian expression vectors (Invitrogen) and ready to be used for generation of recombinant proteins and antibody production.

Download or read book Long Non Coding RNAs in Cancer written by Alfons Navarro and published by Humana. This book was released on 2022-06-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Long Non-Coding RNAs in Cancer aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field.

Download or read book Functional Analysis of Risk Single Nucleotide Polymorphism and Long Noncoding RNA in Prostate Cancer written by Jun Jie Hua and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the most commonly diagnosed non-cutaneous cancer in men worldwide. While most low-risk prostate cancer patients have a favourable clinical outcome, intermediate- and high-risk patients with aggressive forms of the disease often relapse despite the initially effective local and conventional androgen-deprivation therapies. Therefore, two main challenges in the field of prostate cancer are patient stratification into appropriate risk groups through prognostic biomarkers and novel therapeutic options for patients with aggressive prostate cancer. Prostate cancer has a strong genetic component, with Genome-wide Association Studies (GWAS) identifying over 150 risk single nucleotide polymorphisms (SNPs). While these risk SNPs are associated with prostate cancer predisposition and a few also associated with disease progression, their functional mechanisms remain elusive. Here, I evaluated the association of the risk SNP rs11672691 with prostate cancer progression and its clinical relevance, and uncovered its regulatory mechanisms at a novel bifunctional enhancer-promoter element to dysregulate two gene isoforms of a long noncoding RNA (lncRNA) PCAT19. Furthermore, I validated the oncogenic properties of the lncRNA PCAT19, and revealed its function in prostate cancer progression through the upregulation of a subset of cell cycle genes with the RNA binding protein HNRNPAB. My work signifies the clinical importance in understanding the functional mechanisms of prostate cancer risk SNPs and lncRNAs in prostate cancer. Additionally, my work also shed light on the function and molecular mechanisms of a novel class of regulatory elements in the human genome, and its role in cancer aggressiveness.

Download or read book Non coding RNAs and Cancer written by Muller Fabbri and published by Springer Science & Business Media. This book was released on 2013-10-28 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of microRNAs and its role as gene expression regulators in human carcinogenesis represents one of the most important scientific achievements of the last decade. More recently, other non-coding RNAs have been discovered and its implications in cancer are emerging as well, suggesting a broader than anticipated involvement of the non-coding genome in cancer. Moreover, completely new and unexpected functions for microRNAs are being revealed, leading to the identification of new anticancer molecular targets. This book represents a comprehensive guide on non-coding RNAs and cancer, spanning from its role as cancer biomarkers, to providing the most useful bioinformatic tools, to presenting some of the most relevant discoveries, which indicates how these fascinating molecules act as fine orchestrators of cancer biology.

Download or read book MiRNAs and Prostate Cancer written by Annika Fendler and published by . This book was released on 2011 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Functional Characterization of Novel Markers for Prostate Cancer written by Alexander Hermani and published by . This book was released on 2005 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Pistachio Production Manual written by Louise Ferguson and published by UCANR Publications. This book was released on 2016-12-02 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The long-awaited Pistachio Production Manual from the University of California is here! The combined knowledge of 42 UC and industry experts and years of research and field trials are brought to fruition in this long awaited, 321-page manual. From an overview of the state of the industry to physiological disorders, the 8-part manual covers everything you need to know. Chapters cover topics including orchard design; rootstocks and cultivars; planting and training young trees; weed, insect, mite, and vertebrate management; irrigation and salinity management; disease management; and physiological disorders including alternate bearing, nut blanking and shell splitting. Over 200 color photographs and 60 diagrams, charts, and tables illustrate key points. The back cover includes a photographic guide to the developmental stages of the pistachio.

Download or read book Long Non Coding RNA Biology written by M.R.S. Rao and published by Springer. This book was released on 2017-08-16 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume offers a comprehensive and detailed overview of the various aspects of long non-coding RNAs and discusses their emerging significance. Written by leading experts in the field, it motivates young researchers around the globe, and offers graduate and postgraduate students fascinating insights into genes and their regulation in eukaryotes and higher organisms.

Download or read book Identification and Mechanistic Characterization of DLX6 AS1 LncRNA in Prostate Cancer written by Mélanie Weiss and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cancer Genomics written by Hui Ling and published by Elsevier Inc. Chapters. This book was released on 2013-11-21 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of microRNA (miRNA) involvement in cancer a decade ago, and the more recent findings of long non-coding RNAs in human diseases, challenged the long-standing view that RNAs without protein-coding potential are simply “junk” transcription within the human genome. These findings evidently changed the dogma that “DNA makes RNA makes protein” by showing that RNAs themselves can be essential regulators of cellular function and play key roles in cancer development. MiRNAs are evolutionarily conserved short single-stranded transcripts of 19–24 nucleotides in length. They do not code for proteins, but change the final output of protein-coding genes by regulating their transcriptional and/or translation process. Ultraconserved genes (UCGs) are non-coding RNAs with longer length (>200bp) that are transcribed from the ultraconserved genomic region. Both miRNAs and UCGs are located within cancer-associated genomic regions (CAGRs) and can act as tumor suppressors or oncogenes. In this chapter, we present principles and concepts that have been identified over the last decade with respect to our understanding of the function of non-coding RNAs, and summarize recent findings on the role of miRNAs and UCGs in cancer development. Finally, we will conclude by discussing the translational potential of this knowledge into clinical settings such as cancer diagnosis, prognosis and treatment.

Download or read book Molecular Biology of Prostate Cancer written by Manfred Wirth and published by Walter de Gruyter. This book was released on 2013-05-22 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Mechanistic Investigation of Recurrent Functional Genomic and Transcriptional Alterations in Advanced Prostate Cancer written by Thomas Alonzo White and published by . This book was released on 2013 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Novel functionally altered transcripts may be recurrent in prostate cancer (PCa) and may underlie lethal and advanced disease and the neuroendocrine small cell carcinoma (SCC) phenotype. We conducted an RNASeq survey of the LuCaP series of 24 PCa xenograft tumors from 19 men, and validated observations on metastatic tumors and PCa cell lines. Key findings include discovery and validation of 40 novel fusion transcripts including one recurrent chimera, many SCC-specific and castration resistance (CR) -specific novel splice isoforms, new observations on SCC-specific and TMPRSS2-ERG specific differential expression, the allele-specific expression of certain recurrent non-synonymous somatic single nucleotide variants (nsSNVs) previously discovered via exome sequencing of the same tumors, rgw ubiquitous A-to-I RNA editing of base excision repair (BER) gene NEIL1 as well as CDK13, a kinase involved in RNA splicing, and the SCC expression of a previously unannotated long noncoding RNA (lncRNA) at Chr6p22.2. Mechanistic investigation of the novel lncRNA indicates expression is regulated by derepression by master neuroendocrine regulator RE1-silencing transcription factor (REST), and may regulate some genes of axonogenesis and angiogenesis. Taken together these variant transcripts offer insight into aggressive metastatic PCa, both broadly and with regard to castration-resistant and neuroendocrine disease.

Download or read book Control of Leaf Growth written by N. R. Baker and published by CUP Archive. This book was released on 1985 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1985, this book covers the physiological and environmental factors that regulate leaf growth. It opens with a consideration of the importance to the plant of leaf size, form and development, and then divides naturally into two sections: the first covers the intrinsic factors within the leaf that influence development, including solute and hormonal status, cellular components, and energy transducing systems; the second considers the role of some major environmental variables in the regulation of leaf growth, including temperature, light, water and nutrients, atmospheric influences and the interactive effects of climatic variables.

Download or read book Pharmacological Properties of Plant Derived Natural Products and Implications for Human Health written by George E. Barreto and published by Springer Nature. This book was released on 2021-04-16 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicinal plants and their derived products remain as an indispensable source of bioactive molecules that serve as either drug candidates or lead compounds for drug design and discovery. There are several advantages for plant-derived therapeutics including wide availability, diverse pharmacological actions and a generally good profile of safety and tolerability. Over the recent years, there have been numerous reports from clinical studies testifying to the efficacy and safety of medicinal plants and phytochemicals in ameliorating several human diseases. A plethora of basic studies has also unravelled molecular mechanisms underlying the health benefits of herbal medicines. Nevertheless, issues such as identification of bioactive ingredients, standardization of the products and drug interactions remain to be further studied. In this book, we aim to put together several chapters on the medicinal properties and pharmacological action of medicinal plants, plant species and phytochemicals. The goal is to present a comprehensive collection on most of the therapeutic aspects of plant-derived natural products and molecular mechanisms thereof.

Download or read book Small Non Coding RNAs written by Mathieu Rederstorff and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs.