Download or read book Identification and Fine mapping Genomic Regions Associated with Plant Regeneration Reponse in Maize Zea Mays L written by Jason Patrick Cook and published by . This book was released on 2009 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification of Genomic Regions Controlling Maturity in Maize Zea Mays L written by Tae San Kim and published by . This book was released on 1992 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of a Maize zea Mays L Gene Controlling Embryogenic Tissue Culture Response and Enhancement of Maize Transformation Systems written by Frank Lloyd McFarland and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is one of the most economically important crops in the US and globally for food, feed, and biofuel production. Efficient production of regenerable, embryogenic tissue cultures of maize is necessary for both basic biological research and applied biotechnological approaches to maize genetic improvement. Efficient generation of regenerable cultures in maize is highly genotype-dependent and, historically, researchers in academia and industry have relied extensively upon a restrictively small number of genotypes amenable to conventional tissue culture. To identify the genetic basis for genotypic differences in culture response, a mapping-based approach was utilized. The tissue culture response trait from the regenerable maize inbred A188 was backcrossed into the recalcitrant inbred B73. An A188 genomic introgression spanning 3 Mb on chromosome 3 was strongly associated with regenerable tissue culture response. Three candidate genes were selected in the region based on their expression in A188 immature embryos and functional annotations of genes in the region. A screen of each candidate gene expressed under constitutive promoters identified a single candidate, Wox2a, which was found to induce somatic embryogenesis and embryogenic callus in B73.A complementary approach to mapping the A188 culture response trait was undertaken to map the genotype-dependent response of diverse maize lines to Wuschel2/Baby boom (Wus/Bbm)-mediated genetic transformation. Though Wus/Bbm-mediated transformation reduced recalcitrance of many lines through co-transformation with developmental regulators, strong trends of genotype-dependence were still observed. Ten diverse maize lines were screened to identify lines highly amenable to Wus/Bbm-mediated transformation. Transgenic plants/events were recovered from eight of the ten lines. One line, Gaspé Flint, generated transgenic events efficiently and at a wide range of embryo maturities. A Gaspé Flint x B73 introgression library was screened through Wus/Bbm-mediated transformation and Gaspe̹ Flint introgressions associated with transformability were identified on chromosomes 1, 4, and 7. The scale of the transformation experiments necessitated isolation of thousands of immature embryo explants, for which a high-throughput method of embryo isolation (SIMPLE extraction) was developed. Methods for SIMPLE extraction, as well as optimized methods for Wus/Bbm-mediated and Wox2a-mediated maize transformation were developed to enable easy adoption these developing technologies.
Download or read book The Maize Genome written by Jeffrey Bennetzen and published by Springer. This book was released on 2018-11-24 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses advances in our understanding of the structure and function of the maize genome since publication of the original B73 reference genome in 2009, and the progress in translating this knowledge into basic biology and trait improvement. Maize is an extremely important crop, providing a large proportion of the world’s human caloric intake and animal feed, and serving as a model species for basic and applied research. The exceptionally high level of genetic diversity within maize presents opportunities and challenges in all aspects of maize genetics, from sequencing and genotyping to linking genotypes to phenotypes. Topics covered in this timely book range from (i) genome sequencing and genotyping techniques, (ii) genome features such as centromeres and epigenetic regulation, (iii) tools and resources available for trait genomics, to (iv) applications of allele mining and genomics-assisted breeding. This book is a valuable resource for researchers and students interested in maize genetics and genomics.
Download or read book Identification and Characterization of a Maize zea Mays L Gene Controlling Embryogenic Tissue Culture Response and Enhancement of Maize Transformation Systems written by Frank Lloyd McFarland and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is one of the most economically important crops in the US and globally for food, feed, and biofuel production. Efficient production of regenerable, embryogenic tissue cultures of maize is necessary for both basic biological research and applied biotechnological approaches to maize genetic improvement. Efficient generation of regenerable cultures in maize is highly genotype-dependent and, historically, researchers in academia and industry have relied extensively upon a restrictively small number of genotypes amenable to conventional tissue culture. To identify the genetic basis for genotypic differences in culture response, a mapping-based approach was utilized. The tissue culture response trait from the regenerable maize inbred A188 was backcrossed into the recalcitrant inbred B73. An A188 genomic introgression spanning 3 Mb on chromosome 3 was strongly associated with regenerable tissue culture response. Three candidate genes were selected in the region based on their expression in A188 immature embryos and functional annotations of genes in the region. A screen of each candidate gene expressed under constitutive promoters identified a single candidate, Wox2a, which was found to induce somatic embryogenesis and embryogenic callus in B73.A complementary approach to mapping the A188 culture response trait was undertaken to map the genotype-dependent response of diverse maize lines to Wuschel2/Baby boom (Wus/Bbm)-mediated genetic transformation. Though Wus/Bbm-mediated transformation reduced recalcitrance of many lines through co-transformation with developmental regulators, strong trends of genotype-dependence were still observed. Ten diverse maize lines were screened to identify lines highly amenable to Wus/Bbm-mediated transformation. Transgenic plants/events were recovered from eight of the ten lines. One line, Gaspé Flint, generated transgenic events efficiently and at a wide range of embryo maturities. A Gaspé Flint x B73 introgression library was screened through Wus/Bbm-mediated transformation and Gaspé Flint introgressions associated with transformability were identified on chromosomes 1, 4, and 7. The scale of the transformation experiments necessitated isolation of thousands of immature embryo explants, for which a high-throughput method of embryo isolation (SIMPLE extraction) was developed. Methods for SIMPLE extraction, as well as optimized methods for Wus/Bbm-mediated and Wox2a-mediated maize transformation were developed to enable easy adoption these developing technologies.
Download or read book Handbook of Maize written by Jeff L. Bennetzen and published by Springer Science & Business Media. This book was released on 2009-01-16 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is one of the world’s highest value crops, with a multibillion dollar annual contribution to agriculture. The great adaptability and high yields available for maize as a food, feed and forage crop have led to its current production on over 140 million hectares worldwide, with acreage continuing to grow at the expense of other crops. In terms of tons of cereal grain produced worldwide, maize has been number one for many years. Moreover, maize is expanding its contribution to non-food uses, including as a major source of ethanol as a fuel additive or fuel alternative in the US. In addition, maize has been at the center of the transgenic plant controversy, serving as the first food crop with released transgenic varieties. By 2008, maize will have its genome sequence released, providing the sequence of the first average-size plant genome (the four plant genomes that are now sequenced come from unusually tiny genomes) and of the most complex genome sequenced from any organism. Among plant science researchers, maize has the second largest and most productive research community, trailing only the Arabidopsis community in scale and significance. At the applied research and commercial improvement levels, maize has no peers in agriculture, and consists of thousands of contributors worthwhile. A comprehensive book on the biology of maize has not been published. The "Handbook of Maize: the Genetics and Genomics" center on the past, present and future of maize as a model for plant science research and crop improvement. The books include brief, focused chapters from the foremost maize experts and feature a succinct collection of informative images representing the maize germplasm collection.
Download or read book Genetics Genomics and Breeding of Maize written by Ramakrishna Wusirika and published by CRC Press. This book was released on 2014-08-05 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequencing of the maize genome has opened up new opportunities in maize breeding, genetics and genomics research. This book highlights modern trends in development of hybrids, analysis of genetic diversity, molecular breeding, comparative and functional genomics, epigenomicsand proteomics in maize. The use of maize in biofuels, phytoremediation and pharmaceuticals is also highlighted. Current research trends, future research directions and challenges are discussed by a panel of experts from all over the world.
Download or read book Drought Tolerance Traits in Maize Zea Mays at the Early Vegetative Growth Stage written by Abdalla I. Ibrahim and published by . This book was released on 2017 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early seedling vigor and juvenile vegetative growth are important traits that allow the strong establishment of plants and access to nutrients and water, providing competition against weeds, and allowing mechanical cultivation in production systems that do not use herbicides. Drought stress at this early growth stage may be lethal or damaging. We used to the plant Digital Biomass as predicted from digital images to track plant growth under both well-watered and water-stressed conditions. To achieve these goals, we developed a manual imaging system that allowed us to track the plant growth over a period of 32 days. We imaged 30,36 plants representing 449 inbred lines daily from 13 to 32 days after planting with both a top and a side image. The drought treatment started 23 days after planting by completely withholding water from the water-stress treatment. Using Integrated Analysis Platform (IAP) software, we extracted 137 traits from the images including plant architectural traits and color traits. Phenotypic analysis of several traits showed variability across inbreds. Digital Biomass, for example, showed a great variability across inbreds with a 6.6-fold difference at the beginning of the experiment. Digital Biomass, estimated from the top and side images, was shown to be a good measure of plant vigor and strongly correlated with plant shoot weight at harvest. Vigorous seedling utilized more water, reflecting their ability to take advantage of available resources. The value of image-based traits of young plants was evaluated as a predictive tool for adult phenotypes grown in the field. Weak to moderate correlations were obtained between Digital Biomass at the seedling stage, with r-squared values of -0.35, -0.31 for GDD to Anthesis, and GDD to Silking respectively. The correlation between early maize growth and flowering time may suggest a common genetic control of growth and development of both stages with some possible genes with pleiotropic effects. To identify genomic regions associated with the several phenotypic traits, we utilized a dataset of 436,576 SNP markers to conduct Genome-wide Association (GWAS) using the GAPIT package in R. Several candidate genes were identified for growth rate and total leaf area at specific growth stages, as well as for other correlated traits. GWAS of image-derived plant color traits detected genes associated with plant pigments such as anthocyanin and chlorophyll, which confirms earlier reports on the utility of plant imaging in identifying plant pigments. We wanted to test whether growth, as measured by Digital Biomass, was controlled by a fixed or a dynamic set of genes, so we carried out GWAS analysis of Digital Biomass for each day as a separate phenotype. Results have shown that variation for early vegetative growth in maize is controlled by a dynamic set of genes over time, highlighting the importance of repeated measurement over time in GWAS and QTL studies designed to characterize the genetic architecture of plant development. The analysis of the drought-stressed plants showed variability in different drought tolerance traits ranging from 1.2 to 12.2-fold difference. The several measured traits included traits such as 1) leaf expansion sensitivity to water content and traits related to the ability to recover after drought such as 2) surviving green tissue after drought stress, 3) water use efficiency, and 4) growth rate after recovery with. No or weak correlations were found between the plant's ability to tolerate drought and its ability to recover. Photosynthesis Efficiency measured as Fv/Fm on a subset of 140 plants at three time-points during drought stress, showed that photosynthetic efficiency is less sensitive to drought stress than leaf growth. The candidate genes identified in this study, as well as correlations with field agronomic traits, may provide an insight that helps future understanding of the genetic control of biomass-related traits under both well-watered and drought stress conditions.
Download or read book Whole genome Patterns of DNA Variation in Maize written by Michael Allen Gore and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome-Wide Association Studies (GWAS) offer the potential to resolve complex traits to a single gene or an individual polymorphism. GWAS, which rely on historical recombination for resolving complex traits, require that regions of active recombination be genotyped at high-density. Therefore, the molecular focus to the maize (Zea mays L.) genome is to genotype the gene-rich or low-copy-number regions, as these are the preferential sites for meiotic recombination. Due to the rapid rate of linkage disequilibrium decay in a large, diverse genome, it is possible that several million markers are needed for GWAS in diverse maize. The integration of gene-enrichment approaches and high-throughput genotyping platforms offer the potential to score polymorphisms at the needed scale in an efficient and cost-effective manner. The two initial studies focused on developing methodologies to identify and score polymorphisms in large, complex plant genomes. In the first study, four geneenrichment and complexity reduction target preparation methods were tested for scoring polymorphisms on the Affymetrix Maize GeneChip. The results indicated that the tested target preparation methods offered only modest power to detect polymorphisms with the Maize GeneChip. However, 10,000s of informative markers were still discovered. In the second study, gene-enriched genomic libraries constructed for two maize inbred lines were sequenced using massively parallel pyrosequencing. This combined with a computational SNP calling pipeline designed to reduce the number of false positive SNPs resulting from paralogs lead to the identification of more than 120,000 single-nucleotide polymorphisms (SNPs). The third study used Solexa sequencing for low-copy-enrichment resequencing of inbred lines that are the founders of the maize Nested Association Mapping (NAM) population. More than 3 million polymorphisms were scored across the founders, and a substantial portion of the low-copy fraction was highly divergent or novel relative to the reference genome. Recent and ancestral recombination rates were strongly correlated with nucleotide diversity, which suggests that genome structure partly shaped diversity. In addition, we identified regions of the maize genome that are potentially selective sweeps or involved in regional adaptation. These results should be an excellent resource for GWAS, fine-mapping projects, and understanding maize diversity and evolution.
Download or read book The Maize Handbook written by Michael Freeling and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Maize Handbook represents the collective efforts of the maize research community to enumerate the key steps of standard procedures and to disseminate these protocols for the common good. Although the material in this volume is drawn from experience with maize, many of the procedures, protocols, and descriptions are applicable to other higher plants, particularly to other grasses. The power and resolution of experiments with maize depend on the wide range of specialized genetic techniques and marked stocks; these materials are available today as the culmination of nearly 100 years of genetic research. A major goal of this volume is to introduce this genetical legacy and to highlight current stock construction programs that will soon benefit our work, e. g. high-density RFLP maps, deletion stocks, etc. Both stock construction and maintenance are relatively straightforward in maize as a result of the ease of crossing and the longevity of stored seeds. Crossing is facilitated by the separate staminate (tassel) and pistillate (ear) flowers, a feature almost unique to maize. On the other hand, many of the genetic methodologies utilized with maize, including the precision of record keeping, can be adapted to other plants. Facile communication and a spirit of co-operation have characterized the maize genetics community since its earliest days. Starting in the 1930s, institutions such as annual Maize Genetics Cooperation Newsletter, the Maize Genetics Stock Center, and the annual maize genetics meeting provide continuity to the field.
Download or read book Rice Improvement written by Jauhar Ali and published by Springer Nature. This book was released on 2021-05-05 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1990 with total page 1120 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agronomy News written by and published by . This book was released on 1997 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sept.-Oct. issue includes list of theses and dissertations for U.S. and Canadian graduate degrees granted in crop science, soil science, and agronomic science during the previous academic year.
Download or read book Molecular Plant Breeding written by Yunbi Xu and published by CABI. This book was released on 2010 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in plant genomics and molecular biology have revolutionized our understanding of plant genetics, providing new opportunities for more efficient and controllable plant breeding. Successful techniques require a solid understanding of the underlying molecular biology as well as experience in applied plant breeding. Bridging the gap between developments in biotechnology and its applications in plant improvement, Molecular Plant Breeding provides an integrative overview of issues from basic theories to their applications to crop improvement including molecular marker technology, gene mapping, genetic transformation, quantitative genetics, and breeding methodology.
Download or read book Genome Engineering for Crop Improvement written by Bidyut Kumar Sarmah and published by Springer Nature. This book was released on 2021-04-18 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book serves the teachers, researchers and the students as a handy and concise reference as well as guidebook while designing and planning for use of the advanced technologies for crop improvement. The content of the book is designed to cover the latest genome engineering techniques for crop improvement. The conventional breeding has got its limitations such as non-availability of desired genes within the genepool. In many cases, breeding has been highly used and it has nearly reached its highest limit so far as the productivity and production of crops are concerned. However, with increasing need of food and decreasing resources, including water, land, labour, etc., to feed the growing population, the alternative available ways of increasing crop productivity need to be explored and exploited. Genome engineering has a wide scope that includes technologies such as genetic engineering and transgenesis, RNA technologies, CRISPR, cisgenics and subgenics for better productivity and more efficient biotic and abiotic stress management. Therefore, the book is planned to enlighten the readers with the advanced technologies with examples and case studies, whenever possible. Efforts will be made to emphasize on general efforts on various major food crops; however, it would also be made clear that such efforts could be taken as proofs of concepts and that this could be extrapolated keeping the demand in mind.
Download or read book Association Mapping in Plants written by Nnadozie C. Oraguzie and published by Springer Science & Business Media. This book was released on 2007 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the past decade, there has been success in using conventional map-based strategies in identification and cloning of quantitative trait loci (QTL) in model plant species including tomato and Arabidopsis. These quantitative traits are generally the products of many loci with varying degrees of effect upon the observed phenotypes. Recently, a new approach to genetic mapping has emerged called association mapping. This new technique takes into account the thousands of genes to evaluate for QTL effect and is a more efficient approach that does not require generation of segregating populations/large numbers of progeny. As it can utilize all of the historic recombination events in a diverse population of individuals it can generate higher resolution genetic maps and, is needed to complement current map based cloning methods. Association Mapping in Plants provides both basic and advanced understanding of association mapping and an awareness of population genomics tools to facilitate mapping and identification of the underlying causes of quantitative trait variation in plants. It acts as a useful review of the marker technology, the statistical methodology, and the progress to date. It also offers guides to the use of single nucleotide polymorphisms (SNPs) in association studies. This book will appeal to all those with an interest in plant genetics, plant breeding, and plant genomics. About the Editors: Dr. Nnadozie C. Oraguzie is a Senior Scientist in Genetics at the Horticulture and Food Research Institute of New Zealand Ltd (HortResearch). Dr. Erik H. A. Rikkerink is a Science Leader at HortResearch, New Zealand. Dr. Susan E. Gardiner is a Principal Scientist and leader of the Gene Mapping research team at HortResearch, New Zealand. Dr. H. Nihal De Silva/STRONG is a Senior Scientist of Biometrics at HortResearch, New Zealand.
Download or read book Advances in Haploid Production in Higher Plants written by Alisher Touraev and published by Springer Science & Business Media. This book was released on 2008-12-18 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of haploids is well known to geneticists and plant breeders. The discovery of anther-derived haploid Datura plants in 1964 initiated great excitement in the plant breeding and genetics communities as it offered shortcuts in producing highly desirable homozygous plants. Unfortunately, the expected revolution was slow to materialise due to problems in extending methods to other species, including genotypic dependence, recalcitrance, slow development of tissue culture technologies and a lack of knowledge of the underlying processes. Recent years have witnessed great strides in the research and application of haploids in higher plants. After a lull in activities, drivers for the resurgence have been: (1) development of effective tissue culture protocols, (2) identification of genes c- trolling embryogenesis, and (3) large scale and wide spread commercial up-take in plant breeding and plant biotechnology arenas. The first major international symposium on “Haploids in Higher Plants” took place in Guelph, Canada in 1974. At that time there was much excitement about the potential benefits, but in his opening address Sir Ralph Riley offered the following words of caution: “I believe that it is quite likely that haploid research will contr- ute cultivars to agriculture in several crops in the future. However, the more extreme claims of the enthusiasts for haploid breeding must be treated with proper caution. Plant breeding is subject from time to time to sweeping claims from ent- siastic proponents of new procedures.
