Download or read book Identification and Characterisation of Plasmodium Falciparum Variant Surface Antigens Expressed by Parasites Causing Severe Malaria in Children written by Pamela A. Magistrado and published by . This book was released on 2005 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Malaria written by Institute of Medicine and published by National Academies Press. This book was released on 1991-02-01 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is making a dramatic comeback in the world. The disease is the foremost health challenge in Africa south of the Sahara, and people traveling to malarious areas are at increased risk of malaria-related sickness and death. This book examines the prospects for bringing malaria under control, with specific recommendations for U.S. policy, directions for research and program funding, and appropriate roles for federal and international agencies and the medical and public health communities. The volume reports on the current status of malaria research, prevention, and control efforts worldwide. The authors present study results and commentary on the: Nature, clinical manifestations, diagnosis, and epidemiology of malaria. Biology of the malaria parasite and its vector. Prospects for developing malaria vaccines and improved treatments. Economic, social, and behavioral factors in malaria control.
Download or read book Detection of Plasmodium Falciparum written by Renate Zelger and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is one of the most deadly infectious diseases, responsible for 438 000 deaths and 214 million infections per year, mostly in developing countries. Ultra-sensitive detection of the asexual and sexual forms of Plasmodium spp., the causative agent of malaria, is crucial to reduce the staggering numbers of malaria infections with the ultimate goal of eradication, and it is important for research. Unfortunately all current detection methods and biomarkers for malaria have limitations. The performance of antigen-detection assays depends strongly on the biomarker detected, the antibodies used to target the biomarker and the detection method itself. The goal of this project was to improve the detection of Plasmodium falciparum by addressing these three main factors. First, novel biomarkers were identified in a bioinformatics approach, then experimentally characterized and evaluated for their suitability to use in diagnostic tests. KAHRP and PFD1170c were recognized as potential markers for asexual stage parasites. A particular focus was on biomarkers specific for gametocytes, as gametocytes are the only stage able to infect mosquitos, therefore crucial for the spread of the disease and prime targets for transmission blocking strategies. Potential gametocytes specific biomarkers were selected through a transcriptome study of field samples. The candidates were then GFP-tagged for characterization at the protein level. PFC0685w was identified as gametocyte specific biomarker. The detection limit of antigen-detection assays depends on the availability of high affinity antibodies targeting the biomarker. A limiting factor for the generation of high affinity antibodies is the difficulty of expressing Plasmodium antigens required for antibody generation. Dictyostelium discoideum was assessed as an alternative host for the recombinant antigen expression. KAHRP was successfully expressed in Dictyostelium discoideum using an inducible system. An immuno-qPCR, the so-called TT-lock immuno-qPCR was adapted for the quantitative detection of malaria parasites. In general, immuno-qPCR uses a DNA-conjugated antibody to detect a specific antigen by PCR amplification. Its advantage is an unprecedented sensitivity. However, one major challenge in immuno-qPCR is the synthesis of protein-DNA conjugates. The TT-lock immuno-qPCR uses the strong interaction between the DNA-binding protein Tus and a specific DNA sequence for the protein-DNA conjugation. The TT-lock immuno-qPCR has been optimized to detect PfLDH, a widely used biomarker in commercial available rapid diagnostic tests. The assay performed well in detecting recombinant PfLDH, but for the detection of parasite material it did not perform better than already existing methods. The findings of this study highlight the difficulties of improving malaria diagnosis using antigens. To find the right antibodies for a detection method is a challenging task, as each antibody needs to be evaluated experimentally and the generation of new antibodies does not guarantee a higher quality. In hand go the challenges to express recombinant antigen needed for antibody generation. Furthermore, the detection method optimized in this study needs further fine-tuning to push the limit of detection, even after extensive attempts of optimization.
Download or read book World Malaria Report 2019 written by World Health Organization and published by . This book was released on 2019-08-28 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: The World Malaria Report 2019 provides a comprehensive update on global and regional malaria data and trends. The report tracks investments in malaria programs and research as well as progress across all intervention areas: prevention, diagnosis, treatment, elimination, and surveillance. It also includes dedicated chapters on the consequences of malaria on maternal infant and child health the "High Burden to High Impact" approach as well as biological threats to the fight against malaria. The 2019 report is based on information received from more than 80 countries and areas with ongoing malaria transmission. This information is supplemented by data from national household surveys and databases held by other organizations.
Download or read book Identification and Characterization of MB2 written by Thanh Viet Nguyen and published by . This book was released on 2001 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymatic and Chemical Modifications of Erythrocyte Surface Antigens to Identify Plasmodium Falciparum Merozoite Binding Sites written by Kim Lisa Baron and published by . This book was released on 2014 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is a disease caused by the protozoan parasite Plasmodium where the species that causes the most severe form of malaria in humans is known as Plasmodium falciparum. At least 40% of the global population is at risk of contracting malaria with 627 000 people dying as a result of this disease in 2012. Approximately 90% of all malaria deaths occur in sub-Saharan Africa, where approximately every 30 seconds a young child dies, making malaria the leading cause of death in children under the age of five years old. The malaria parasite has a complex life cycle utilising both invertebrate and vertebrate hosts across sexual and asexual stages. The erythrocyte invasion stage of the life cycle in the human whereby the invasive merozoite form of the parasite enters the erythrocyte is a central and essential step, and it is during this stage that the clinical symptoms of malaria manifest themselves. Merozoites invade erythrocytes utilising multiple, highly specific receptor-ligand interactions in a series of co-ordinated events. The aim of this study was to better understand the interactions occurring between the merozoite and erythrocyte during invasion by using modern, cutting-edge proteomic techniques. This was done in the hope of laying the foundation for the discovery of new key therapeutic targets for antimalarial drug and vaccine-based strategies, as there is currently no commercially available antimalarial vaccine and no drug to which the parasite has not at least started showing resistance. In this study healthy human erythrocytes were treated separately with different protein-altering enzymes and chemicals being trypsin, the potent oxidant sodium periodate (NaIO4), the amine cross-linker tris(2-chloroethyl)amine hydrochloride (TCEA) and the thiol cross-linker 1,11-bis(maleimido)triethylene glycol (BM(PEG)3). The resulting erythrocyte protein alterations were visualised as protein band differences on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE), where treated and untreated control erythrocyte ghost protein fingerprints were visually compared to one another. The protein bands showing differences between treated and control samples were in-gel digested using trypsin then sequenced by liquid chromatography tandem mass spectrometry (LC-MS/MS) and identified using proteomics-based software. In this way, the erythrocyte proteins altered by each enzyme/chemical treatment were identified. Malaria invasion assays were performed where each treatment group of erythrocytes as well as the control erythrocytes were incubated separately with schizont stage malaria parasites for the duration of one complete life cycle. Using fluorescent staining and flow cytometry, the invasion inhibition efficiency for each treatment group was evaluated. By utilising these methods, the identification and the relative importance of the erythrocyte proteins involved in the invasion process were determined. Protein fingerprints of control and treated erythrocyte ghosts were visualised and optimised on SDS PAGE where induced protein band differences were successfully identified by LC-MS/MS. It was found that each treatment altered erythrocyte proteins with changes found in Band 3, actin, phosphoglycerate kinase 1, spectrin alpha, spectrin beta, ankyrin, haemoglobin, Bands 4.1 and 4.2, glycophorin A and stomatin. The invasion assays revealed that TCEA inhibited invasion to the greatest extent as compared to the other treatments, followed by BM(PEG)3 and trypsin. Sodium periodate-treated erythrocytes could not be assessed using the invasion assay due to auto-haemolysis. Band 3, glycophorin A, Band 4.1 and stomatin appear to be of higher relative importance in the invasion process as compared to the other altered erythrocyte proteins. These results confirmed the known roles of spectrin alpha, spectrin beta, glycophorin A, Band 3 and Band 4.1 in invasion, and suggested that ankyrin, Band 4.2 and stomatin may also be involved. This study highlighted the potential that modern, cutting-edge proteomic techniques provide when applied to previous comparative studies found in older literature, as previously unidentified proteins that can be involved in invasion were revealed. These results can be used as a foundation in future studies in order to identify new key targets for the development of new antimalarial drug- and vaccine-based strategies, with the hope of preventing the suffering of the millions of malaria-inflicted people worldwide, and ultimately eradicating this deadly disease.
Download or read book Identification of Malaria Parasite infected Red Blood Cell Aptamers by Inertial Microfluidics SELEX written by Christina Marie Birch and published by . This book was released on 2015 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria kills over 500,000 people annually, the majority of whom are children under five years old in sub-Saharan Africa. This disease is caused by several parasite species, of which Plasmodium falciparum is associated with the highest mortality. The clinical manifestations of malaria are associated with the phase of infection where parasites develop within red blood cells (RBCs). Infected RBCs can adhere to the host microvasculature, triggering inflammatory responses in affected organs that contribute to the pathophysiology of life threatening cerebral malaria and pregnancy-associated malaria. The expression of specific Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) variants on the RBC surface is associated with severe disease, such as VAR2CSA-mediated placental sequestration during pregnancy-associated malaria. While parasite proteins expressed on the surface of infected RBCs are linked to disease pathogenesis, this surface proteome is poorly characterized. Identifying parasite-derived antigens on the infected RBC surface could facilitate diagnosis, monitoring, and prevention of sequestration. To interrogate the infected RBC surface proteome, we require a panel of affinity reagents that robustly distinguish the parasite-derived proteins from the elaborate RBC surface milieu. Nucleic acid aptamers are widely used in biological applications for their high specificity and affinity to targets and are highly suitable for malaria applications. Efficiently generating aptamers against complex targets-such as whole cells-remains a challenge. Here we develop a novel strategy (I-SELEX) that utilizes inertial focusing in spiral microfluidic channels to stringently partition cells from unbound oligonucleotides. We use I-SELEX to efficiently discover high affinity aptamers that selectively recognize distinct epitopes present on target cells. Using first an engineered RBC model displaying a non-native antigen and, second, live malaria parasite-infected RBCs as targets, we establish suitability of this strategy for de novo aptamer selections. We demonstrate recovery of a diverse set of aptamers that recognize distinct epitopes on parasite-infected RBCs with nanomolar affinity, including an aptamer against the protein responsible for placental sequestration, VAR2CSA. These findings validate I-SELEX as a broadly applicable aptamer discovery platform that enables identification of new reagents for mapping the parasite-infected RBC surface proteome at higher molecular resolution to potentially contribute to malaria diagnostics, therapeutics and vaccine efforts.
Download or read book Encyclopedia of Malaria written by and published by Springer. This book was released on 2018-07-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of Malaria represents a vast databank of information about the study of malaria. It provides an overview of the historical, rapid and significant developments that have occurred in malaria research, including the 2002 genome sequencing of Plasmodium falciparum and its mosquito vector, Anopheles gambiae. This work provides a concise source of up-to-date research findings in the form of definitions and essays and present comprehensive coverage of topics from history to findings to diagnosis and treatment, written by recognized malaria researchers with practical experience. It appeals to a diverse audience, including malaria researchers, teachers, investigators and public health professionals.
Download or read book Management of Severe Malaria written by World Health Organization and published by World Health Organization. This book was released on 2000-04 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria continues to be a major health problem in many parts of the world, with over 2,400 million people in 100 countries at risk of infection. This handbook is an updated edition of 'Management of severe and complicated malaria', providing practical guidance on the diagnosis and management of severe falciparum malaria, a form of the disease that can have life-threatening complications if treatment is delayed.
Download or read book The Malaria Genome Projects written by Irwin W. Sherman and published by World Scientific. This book was released on 2012 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: The year 2012 marks the tenth anniversary of the announcement of the genome sequence of the human malaria parasite Plasmodium falciparum and that of its mosquito vector Anopheles. The genome sequences were a result of the Plasmodium falciparum Genome Project. This book covers in detail the biology of malaria parasites and the mosquitoes that transmit the disease, how the Genome Project came into being, the people who created it, and the cadre of scientists who are attempting to see the promise of the Project realized. The promise was: a more complete understanding of the genes of the parasite (and its vector) would provide a rational basis for the development of antimalarial drugs and vaccines, allow a better understanding of the regulation of the complex life cycle in the red blood and liver cells of the human, identify the genes the parasite uses to thwart the host immune response and the ways in which the parasite evades cure by drug treatments, as well as leading to more effective measures of control transmission. The hope was that cracking the genetic code of Plasmodium and Anopheles would reveal the biochemical Achilles heel of the parasite and its vector, leading to the development of novel drugs and better methods of control, and by finding the targets of protective immunity could result in the manufacture of effective vaccines. Through a historic approach, this book will allow for those new to the field, or those with insufficient background in the sciences, to have an easier entry point. Even scientists already working in the field may better appreciate how discoveries made in the past can impact the direction of future research.
Download or read book Plasmodium Falciparum Erythrocyte Membrane Protein 1 PfEMP1 Antigens Expressed by Parasites Causing Severe Malaria written by Louise Jørgensen and published by . This book was released on 2007 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immune Recognition of Parasite dependent Antigens Associated with Plasmodium Falciparum infected Erythrocytes written by Hassan Mohamed Elsaid and published by . This book was released on 1988 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Emerging and Re emerging Infections written by Sunit Kumar Singh and published by John Wiley & Sons. This book was released on 2015-11-09 with total page 2365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Emerging and re-emerging pathogens pose several challenges to diagnosis, treatment, and public health surveillance, primarily because pathogen identification is a difficult and time-consuming process due to the “novel” nature of the agent. Proper identification requires a wide array of techniques, but the significance of these diagnostics is anticipated to increase with advances in newer molecular and nanobiotechnological interventions and health information technology. Human Emerging and Re-emerging Infections covers the epidemiology, pathogenesis, diagnostics, clinical features, and public health risks posed by new viral and microbial infections. The book includes detailed coverage on the molecular mechanisms of pathogenesis, development of various diagnostic tools, diagnostic assays and their limitations, key research priorities, and new technologies in infection diagnostics. Volume 1 addresses viral and parasitic infections, while volume 2 delves into bacterial and mycotic infections. Human Emerging and Re-emerging Infections is an invaluable resource for researchers in parasitologists, microbiology, Immunology, neurology and virology, as well as clinicians and students interested in understanding the current knowledge and future directions of infectious diseases.
Download or read book Hunter s Tropical Medicine and Emerging Infectious Diseases E Book written by Edward T Ryan and published by Elsevier Health Sciences. This book was released on 2019-03-25 with total page 1264 pages. Available in PDF, EPUB and Kindle. Book excerpt: New emerging diseases, new diagnostic modalities for resource-poor settings, new vaccine schedules ... all significant, recent developments in the fast-changing field of tropical medicine. Hunter’s Tropical Medicine and Emerging Infectious Diseases, 10th Edition, keeps you up to date with everything from infectious diseases and environmental issues through poisoning and toxicology, animal injuries, and nutritional and micronutrient deficiencies that result from traveling to tropical or subtropical regions. This comprehensive resource provides authoritative clinical guidance, useful statistics, and chapters covering organs, skills, and services, as well as traditional pathogen-based content. You’ll get a full understanding of how to recognize and treat these unique health issues, no matter how widespread or difficult to control. Includes important updates on malaria, leishmaniasis, tuberculosis and HIV, as well as coverage of Ebola, Zika virus, Chikungunya, and other emerging pathogens. Provides new vaccine schedules and information on implementation. Features five all-new chapters: Neglected Tropical Diseases: Public Health Control Programs and Mass Drug Administration; Health System and Health Care Delivery; Zika; Medical Entomology; and Vector Control – as well as 250 new images throughout. Presents the common characteristics and methods of transmission for each tropical disease, as well as the applicable diagnosis, treatment, control, and disease prevention techniques. Contains skills-based chapters such as dentistry, neonatal pediatrics and ICMI, and surgery in the tropics, and service-based chapters such as transfusion in resource-poor settings, microbiology, and imaging. Discusses maladies such as delusional parasitosis that are often seen in returning travelers, including those making international adoptions, transplant patients, medical tourists, and more.
Download or read book Trafficking and Biochemical Characterization of Plasmodium Falciparum Maurer s Cleft Two Transmembrane Protein written by Raghavendra Yadavalli and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmodium falciparum virulence proteins and molecules required for assembly of the knobs used for cytoadherence are exported through the Maurer's clefts which are formed in the erythrocyte cytoplasm immediately following merozoite invasion into the erythrocyte host cell. Proteins participating in knob formation, cytoadherence or immune evasion include variant-surface antigens (VSAs) that are trafficked alone or chaperoned by other parasite proteins through the Maurer's clefts. In this study, recombinant PfMC-2TM was expressed using HeLa based in vitro human cell free expression system. Further, the stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in the parasite and erythrocyte cytoplasm was investigated in 3D7 and FC-27 strains of P. falciparum. Detailed cell biological and biochemical characterizations were performed to identify the mode of PfMC-2TM export from the parasite to erythrocyte cytoplasm. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence and western blotting with antibodies reactive with PfMC-2TM. Our data shows that PfMC-2TM is sensitive to BFA treatment in the ring and trophozoite stage. Permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with Maurer's cleft resident protein REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associates with the infected erythrocyte membrane as an integral membrane protein and is seen to be transiently exposed on the infected red blood cell surface. PfMC-2TM remains insoluble upon association with the MC and erythrocyte membrane, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs, that can be used as a clinical marker of early blood stage infection.
Download or read book World Malaria Report 2015 written by World Health Organization and published by World Health Organization. This book was released on 2016-01-30 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: The World Malaria Report 2015assesses global malaria disease trends and changes in the coverage and financing of malaria control programs between 2000 and 2015. It also summarizes progress towards international targets, and provides regional and country profiles that summarize trends in each WHO region and each country with malaria. The report is produced with the help of WHO regional and country offices, ministries of health in endemic countries, and a broad range of other partners. The data presented are assembled from the 96 countries and territories with ongoing malaria transmission, and a further five countries that have recently eliminated malaria. Most data are those reported for 2014 and 2015, although in some cases projections have been made into 2015, to assess progress towards targets for 2015.
Download or read book World Malaria Report 2018 written by World Health Organization and published by World Health Organization. This book was released on 2019-02-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: This year s report shows that after an unprecedented period of success in global malaria control progress has stalled. Data from 2015?2017 highlight that no significant progress in reducing global malaria cases was made in this period. There were an estimated 219 million cases and 435 000 related deaths in 2017. The World malaria report 2018 draws on data from 90 countries and areas with ongoing malaria transmission. The information is supplemented by data from national household surveys and databases held by other organizations.
