Download or read book Identification and Characterisation of a Novel Plasmodium Falciparum Protein written by and published by . This book was released on 2007 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of a Moved Plasmodium Falciparum Protein written by Louise Hinds and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of an FK506 binding Protein from Plasmodium Falciparum written by Paul Monaghan and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of HAEM Interacting Proteins from the Malaria Parasite Plasmodium Falciparum written by Naomi Vittoria Campanale and published by . This book was released on 2007 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Novel Drug Resistance Loci in Plasmodium Falciparum written by Daria Natalie Van Tyne and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria has plagued mankind for millennia. Antimalarial drug use over the last century has generated highly drug-resistant parasites, which amplify the burden of this disease and pose a serious obstacle to control efforts. This dissertation is motivated by the simple fact that malaria parasites have become resistant to nearly every antimalarial drug that has ever been used, yet the precise genetic mechanisms of parasite drug resistance remain largely unknown. Our work pairs genomics-age technologies with molecular biology, genetics and molecular epidemiology in order to identify and characterize novel genes that contribute to drug resistance in P. falciparum.

Download or read book Identification and Characterization of Invasion related Proteins of the Malaria Parasite Plasmodium Falciparum Welch 1892 written by Louisa Wilcke and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Detection of Plasmodium Falciparum written by Renate Zelger and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is one of the most deadly infectious diseases, responsible for 438 000 deaths and 214 million infections per year, mostly in developing countries. Ultra-sensitive detection of the asexual and sexual forms of Plasmodium spp., the causative agent of malaria, is crucial to reduce the staggering numbers of malaria infections with the ultimate goal of eradication, and it is important for research. Unfortunately all current detection methods and biomarkers for malaria have limitations. The performance of antigen-detection assays depends strongly on the biomarker detected, the antibodies used to target the biomarker and the detection method itself. The goal of this project was to improve the detection of Plasmodium falciparum by addressing these three main factors. First, novel biomarkers were identified in a bioinformatics approach, then experimentally characterized and evaluated for their suitability to use in diagnostic tests. KAHRP and PFD1170c were recognized as potential markers for asexual stage parasites. A particular focus was on biomarkers specific for gametocytes, as gametocytes are the only stage able to infect mosquitos, therefore crucial for the spread of the disease and prime targets for transmission blocking strategies. Potential gametocytes specific biomarkers were selected through a transcriptome study of field samples. The candidates were then GFP-tagged for characterization at the protein level. PFC0685w was identified as gametocyte specific biomarker. The detection limit of antigen-detection assays depends on the availability of high affinity antibodies targeting the biomarker. A limiting factor for the generation of high affinity antibodies is the difficulty of expressing Plasmodium antigens required for antibody generation. Dictyostelium discoideum was assessed as an alternative host for the recombinant antigen expression. KAHRP was successfully expressed in Dictyostelium discoideum using an inducible system. An immuno-qPCR, the so-called TT-lock immuno-qPCR was adapted for the quantitative detection of malaria parasites. In general, immuno-qPCR uses a DNA-conjugated antibody to detect a specific antigen by PCR amplification. Its advantage is an unprecedented sensitivity. However, one major challenge in immuno-qPCR is the synthesis of protein-DNA conjugates. The TT-lock immuno-qPCR uses the strong interaction between the DNA-binding protein Tus and a specific DNA sequence for the protein-DNA conjugation. The TT-lock immuno-qPCR has been optimized to detect PfLDH, a widely used biomarker in commercial available rapid diagnostic tests. The assay performed well in detecting recombinant PfLDH, but for the detection of parasite material it did not perform better than already existing methods. The findings of this study highlight the difficulties of improving malaria diagnosis using antigens. To find the right antibodies for a detection method is a challenging task, as each antibody needs to be evaluated experimentally and the generation of new antibodies does not guarantee a higher quality. In hand go the challenges to express recombinant antigen needed for antibody generation. Furthermore, the detection method optimized in this study needs further fine-tuning to push the limit of detection, even after extensive attempts of optimization.

Download or read book Identification and Characterisation of Multidrug Resistance associated Proteins MRPs in Human Erythrocytes and Plasmodium Falciparum written by Antonios Klokouzas and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterisation of Plasmodium Falciparum Variant Surface Antigens Expressed by Parasites Causing Severe Malaria in Children written by Pamela A. Magistrado and published by . This book was released on 2005 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Vesicle Targeting in Plasmodium Falciparum written by Lawrence Sumanjah Ayong and published by . This book was released on 2009 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins of the SNARE (Soluble N-ethylmaleimide sensitive factor attachment protein receptor) super-family have been characterized as playing an essential role in vesicle targeting and fusion in all eukaryotes. The intracellular malaria parasite Plasmodium falciparum exhibits an unusual endomembrane system that is characterized by an unstacked Golgi apparatus, a developmentally induced apical complex, and various organellar structures of parasite origin in the infected host cells. How malaria parasites target nuclear-encoded proteins to these novel compartments is a central question in Plasmodium cell biology. Ultrastructural studies elsewhere have implicated the participation of specialized vesicular elements in transport of virulence proteins, including various cytoadherance and host cell remodeling factors, into the infected erythrocyte cytoplasm. However, little is known about the machineries that define the directionality of vesicle trafficking in malaria parasites. We hypothesized that the P. falciparum SNARE proteins would exhibit novel features required for vesicle targeting to the parasite-specific compartments. We then identified for the first time and confirmed the expression of eighteen SNARE genes in P. falciparum. Members of the PfSNAREs exhibit atypical structural features (Ayong et al., 2007, Molecular & Biochemical Parasitology, 152(2), 113-122). Among the atypical PfSNAREs, PfSec22 contains an unusual insertion of the Plasmodium export element (PEXEL) within its profilin-like longin domain, preceded by an N-terminal hydrophobic segment.

Download or read book Characterisation of Novel Nuclear Proteins in Plasmodium Falciparum written by Andreas Pulfer and published by . This book was released on 2010 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Novel Plasmodium Falciparum Cyclophilins and Their Roles in the Antimalarial Actions of Cyclosporin A written by Alejandro Marín Menéndez and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification of Novel Proteins on the Surface of Plasmodium Falciparum Infected Erythrocytes written by Weidong Zhu and published by . This book was released on 2004 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of MB2 written by Thanh Viet Nguyen and published by . This book was released on 2001 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification of Binding Partners of a Novel P Falciparum Exported Protein written by Mónica Adriana Zapata and published by . This book was released on 2009 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmodium falciparum is the causative agent of the most severe form of malaria. This parasite drastically modifies its host cell, the erythrocyte, to create a hospitable environment for its growth and reproduction. In order for these modifications to occur, the parasite secretes proteins into the erythrocyte. While the secretion machinery is still unknown, many secreted proteins have been found to have a hydrophobic signal sequence and a novel host-targeting signal downstream of the hydrophobic sequence. A novel P. falciparum protein has been shown to be secreted from the parasitophorous vacuole, yet it lacks both a hydrophobic signal sequence and a host-targeting signal. It was hypothesized that this protein, Pfl2110c, must interact with other proteins as it migrates into the erythrocyte. Using immunofluorescence assays and co-immunoprecipitation experiments, I found that Pfl2110c interacts with several parasite proteins as well as with the erythrocyte cytoskeleton. Therefore, Pfl2110c was renamed skeleton-binding protein 2 (SBP-2).System requirements: World Wide Web browser, and PDF reader.

Download or read book Recombinant Espression and Immunological Characterisation of a Novel Protein orf 5 of the Malaria Parasite Plasmodium Falciparum written by Niels Pfeiffer and published by . This book was released on 2002 with total page 73 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterisation of Three Novel Nuclear Proteins in Plasmodium Falciparum written by Stefan Schneitter and published by . This book was released on 2012 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: