Download or read book Human Mesenchymal Stem Cell Morphology Migration And Differentiation On Micro And Nano Textured Titanium written by Jennifer M. Schneider and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell-material interactions are of particular interest in biomedical implants because early events between the cells and the biomaterial can define the success of the device. Upon implantation, bacteria compete with mesenchymal stem cells to attach at the implant surface resulting in either biofilm formation or bone tissue integration. The chosen material and surface topography are an integral part of this initial interaction. Previous work has demonstrated the topography of Ti6Al4V has the ability to regulate the early attachment, migration and differentiation of human mesenchymal stem cells (hMSCs). Specifically, titanium surfaces exhibiting macro- micro roughness resulted in improved cell spreading, random migration, and increased differentiation towards an osteoblastic lineage when compared with smooth topographies (both Ti6Al4V and PEEK). The goal of the current investigation was to further characterize the morphology, migration and differentiation of hMSCs on smooth topographies (both Ti6Al4V and PEEK), macro-micro rough topographies (Endoskeleton) and macro-micro-nano rough topographies (nanoLOCK). The primary hypothesis is that the introduction of nano-topography on micro-rough Ti6Al4V will promote improved cell attachment, migration and differentiation. A secondary hypothesis seeks to identify a correlation between the hMSC morphology at early time-points and long-term differentiation.Together the data presented will validate our two initial hypotheses. Notably, adding a nano-roughness to micro-roughened Ti6Al4V improves the velocity of migrating hMSCs in addition to the increasing the rate at which the hMSCs differentiate to osteoblasts. Furthermore, this improved differentiation appears to be correlated to a rapid adoption of a stellate morphology by the hMSCs on the nanoLOCK surfaces. Thus, these results support the combined macro-micro-nano topography of the nanoLOCK surface as being a substantial improvement over all other spinal fusion cage materials and topographies as demonstrated through rapid random migration necessary to cover a surface, the evolution of a morphology typical of mature osteoblasts/osteocytes, and a rapid progression through the early and mid-osteogenic differentiation markers, ALP and OSX respectively.
Download or read book Modulation of Osteogenic Differentiation in Human Mesenchymal Stem Cells by Submicron Topographically patterned Ridges and Grooves written by Shinya Watari and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent studies have shown that nanoscale and submicron topographic cues modulate a menu of fundamental cell behaviors, and the use of topographic cues is an emerging area of tissue engineering. We used topographically-patterned substrates containing anisotropically-ordered ridges and grooves to mimic one feature of the biophysical environment of type I collagen, which consists of aligned nano- to submicron-scale fibrils. We investigated the effects of topographic cues on mesenchymal stem cell morphology, proliferation, and osteogenic differentiation. Cells cultured on 1400 or 4000 nm pitches showed greater elongation and alignment relative to 400 nm pitch or planar control. Cells cultured on 400 nm pitch demonstrated significant increases in RUNX2 and BGLAP expression relative to cells cultured on 1400 or 4000 nm pitch or planar control. 400 nm pitch enhanced extracellular calcium deposition. Cells cultured in osteoinductive medium revealed combinatory effects of topography and chemical cues on 400 nm pitch as well as up-regulation of expression of ID1, a target of the BMP pathway. Our data demonstrate that a specific size scale of topographic cue promotes osteogenic differentiation with or without osteogenic agents. These data demonstrate that the integration of topographic cues may be useful for the fabrication of orthopedic implants.
Download or read book Mesenchymal Stem Cells Basics and Clinical Application II written by Birgit Weyand and published by Springer. This book was released on 2014-07-08 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered MSCs from Patient-Specific iPS Cells, by Irina Eberle, Mohsen Moslem, Reinhard Henschler, Tobias Cantz Fate of Intravenously Injected Mesenchymal Stem Cells and Significance for Clinical Application, by Beate Wagner, Reinhard Henschler The Implications of Stem Cell Applications for Diseases of the Respiratory System, by Mei Ling Lim, Philipp Jungebluth, Paolo Macchiarini Potential of Mesenchymal Stem Cell Applications in Plastic and Reconstructive Surgery, by Birgit Weyand, Peter M. Vogt General Principles for the Regeneration of Bone and Cartilage, by Michael Jagodzinski, C. Haasper Adult Mesenchymal Stem Cells Explored in the Dental Field, by K. M. Fawzy El-Sayed, C. Dörfer, F. Fändrich, F. Gieseler, M. H. Moustafa, H. Ungefroren Mesenchymal Stem Cell Therapy and Lung Diseases, by Khondoker M. Akram, Sohel Samad, Monica Spiteri, Nicholas R. Forsyth Mesenchymal Stem Cells as Cellular Immunotherapeutics in Allogeneic Hematopoietic Stem Cell Transplantation, by Claudia Papewalis, Daniela Topolar, Barbara Götz, Stefan Schönberger, Dagmar Dilloo New Cell-Based Therapy Paradigm: Induction of Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells into Pro-Inflammatory MSC1 and Anti-inflammatory MSC2 Phenotypes, by Aline M. Betancourt Interactions Between Mesenchymal Stem Cells and Dendritic Cells, by Grazia Maria Spaggiari, Lorenzo Moretta MSC and Tumors: Homing, Differentiation, and Secretion Influence Therapeutic Potential, by Naomi D’souza, Jorge Sans Burns, Giulia Grisendi, Olivia Candini, Elena Veronesi, Serena Piccinno, Edwin M. Horwitz, Paolo Paolucci, Pierfranco Conte, Massimo Dominici Sources of Mesenchymal Stem Cells: Current and Future Clinical Use, by Michela Pozzobon, Martina Piccoli, Paolo De Coppi Role of the EU Framework in Regulation of Stem Cell-Based Products, by Giovanni Migliaccio, Cristina Pintus
Download or read book Mesenchymal Stem Cells Basics and Clinical Application I written by Birgit Weyand and published by Springer. This book was released on 2014-07-08 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prospective Isolation and Characterization of Human Bone Marrow-Derived MSCs, by A. Harichandan, K. Sivasubramaniyan, H.-J. Bühring Urine as a Source of Stem Cells, by Christina Benda, Ting Zhou, Xianming Wang, Weihua Tian, Johannes Grillari, Hung-Fat Tse, Regina Grillari-Voglauer, Duanqing Pei, Miguel A. Esteban Expansion of Mesenchymal Stem/Stromal Cells under Xenogenic-Free Culture Conditions, by Sven Kinzebach, Karen Bieback Adipose-Derived Mesenchymal Stem Cells: Biology and Potential Applications, by Danielle Minteer, Kacey G Marra, J Peter Rubin Potential for Osteogenic and Chondrogenic Differentiation of MSC, by Antonina Lavrentieva, Tim Hatlapatka, Anne Neumann, Birgit Weyand, Cornelia Kasper Potential for Neural Differentiation of Mesenchymal Stem Cells, by Letizia Ferroni, Chiara Gardin, Ilaria Tocco, Roberta Epis, Alessandro Casadei, Vincenzo Vindigni, Giuseppe Mucci, Barbara Zavan Migratory Properties of Mesenchymal Stem Cells, by Thomas Dittmar, Frank Entschladen Dissecting Paracrine Effectors for Mesenchymal Stem Cells, by Stefania Bruno, Federica Collino, Ciro Tetta, Giovanni Camussi Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells, by Yin Xiao, Jiezhong Chen Does the Adult Stroma Contain Stem Cells?, by Richard Schäfer
Download or read book Micro and Nanotechnologies in Engineering Stem Cells and Tissues written by Murugan Ramalingam and published by John Wiley & Sons. This book was released on 2013-05-10 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: A cutting-edge look at the application of micro and nanotechnologies in regenerative medicine The area at the interface of micro/nanotechnology and stem cells/tissue engineering has seen an explosion of activity in recent years. This book provides a much-needed overview of these exciting developments, covering all aspects of micro and nanotechnologies, from the fundamental principles to the latest research to applications in regenerative medicine. Written and edited by the top researchers in the field, Micro and Nanotechnologies in Engineering Stem Cells and Tissues describes advances in material systems along with current techniques available for cell, tissue, and organ studies. Readers will gain tremendous insight into the state of the art of stem cells and tissue engineering, and learn how to use the technology in their own research or clinical trials. Coverage includes: Technologies for controlling or regulating stem cell and tissue growth Various engineering approaches for stem cell, vascular tissue, and bone regeneration The design and processing of biocompatible polymers and other biomaterials Characterization of the interactions between cells and biomaterials Unrivaled among books of this kind, Micro and Nanotechnologies in Engineering Stem Cells and Tissues is the ultimate forward-looking reference for researchers in numerous disciplines, from engineering and materials science to biomedicine, and for anyone wishing to understand the trends in this transformative field.
Download or read book Frontiers in Stem Cell and Regenerative Medicine Research Volume 10 written by Atta-ur-Rahman and published by Bentham Science Publishers. This book was released on 2022-03-03 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frontiers in Stem Cell and Regenerative Medicine Research Volume 10 [Edited for Volume 10] Stem cell and regenerative medicine research is an important area of clinical research which promises to change the face of medicine as it will be practiced in the years to come. Challenges in the 21st century to combat diseases such as cancer, Alzheimer’s disease and retinal disorders, among others, may well be addressed employing stem cell therapies and tissue regeneration techniques. Frontiers in Stem Cell and Regenerative Medicine Research brings updates on multidisciplinary topics relevant to stem cell research and their application in regenerative medicine. The series is essential reading for researchers seeking updates in stem cell therapeutics and regenerative medicine. Volume 10 includes 5 chapters on these topics: -Novel drugs and their stem cell-based targets for osteoporosis: challenges and proceedings -The role of cancer stem cells in disease progression and therapy resistance -Stem cells from human exfoliated deciduous teeth in tissue regeneration -The fate of toxicological studies: from animal models to stem cell-based methods -Effect of material properties on differentiation of mesenchymal stem cells
Download or read book Genetic Engineering of Mesenchymal Stem Cells written by Jan A. Nolta and published by Springer Science & Business Media. This book was released on 2006-08-29 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: MSC (mesenchymal stem cells) have been reported to initiate revascularization after injury, to facilitate engraftment of blood-forming stem cells, and to reduce the incidence of graft-vs. host disease through their immune-suppressive qualities. Finally, bone marrow-derived MSC have been reported to home to areas of solid tumor revascularization, and thus may be used as delivery vehicles to target ablative agents into dividing tumor cells. Recently the characteristics of human MSC from adipose (fat) tissue have also been identified. The possibility of repairing tissues, speeding stem cell engraftment, and targeting solid tumors for specific killing, using MSC easily harvested from bone marrow, or better yet, from unwanted fat tissue, holds broad appeal, and is an intriguing possibility that could have dramatic effect on health care. This book has information on how to isolate, grow, and characterize MSC from marrow and fat, and gives important insight into how these cells may be used for gene delivery and cellular therapies in the future. Updates on emerging clinical trials are given.
Download or read book Advanced Biomaterials and Systems Releasing Bioactive Agents for Precise Tissue Regeneration written by Rui Guo and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Mechanics Regulate Mesenchymal Stem Cell Morphology and T Cell Activation written by Luis Santos and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 3, I analyze how matrix chemistry and substrate rigidity affect human mesenchymal stem cell morphology in the context of lineage differentiation, and speculate on potential mechanisms that cells use to sense local rigidity. In Chapter 4, I present a new substrate design that facilitates live visualization of the interface formed between a T cell and an antigen presenting cell, i.e. the immunological synapse, and discuss the impact of intercellular forces on T cell activation. In Chapter 5, I explore the molecular mechanism of Cas-L mechanical activation at the immunological synapse of T cells, and demonstrate how Cas-L regulates T cell activation in the context of an immune response. Finally, in Chapter 6, I lay down the main conclusions of the thesis, and discuss ongoing projects that directly follow up on the results of this thesis.
Download or read book Mechanical and Biological Mechanisms of Regulating Human Mesenchymal Stem Cell Differentiation written by Anne Kathryn Staples and published by . This book was released on 2006 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of how hMSCs respond to different types of mechanical loading, how this response differs from a traditional growth factor approach of inducing cellular differentiation and how their responsiveness to mechanical stimulation varies with cell differentiation stage are all critical for the successful design of tissue engineering constructs that are optimally organized for a specific mechanical function.
Download or read book Controlling Shape and Differentiation of Human Mesenchymal Stem Cells by Mechanical Input Energy written by Franziska Schäfer and published by . This book was released on 2021* with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Osteogenic Differentiation of Human Mesenchymal Stem Cells by the Design of a Natural Microenvironment written by Sonja Kreß and published by . This book was released on 2012 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Hierarchically structured biomaterials for tissue regeneration written by Wenping Ma and published by OAE Publishing Inc.. This book was released on 2024-03-12 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Repairing tissue defects caused by diseases and traumas presents significant challenges in the clinic. Recent advancements in biomaterials have offered promising strategies for promoting tissue regeneration. In particular, the exploration of 3D macro and microstructures of biomaterials has proven crucial in this process. The integration of macro, micro, and nanostructures facilitates the performance of biomaterials in terms of their mechanical properties, degradation rate, and distinctive impacts on cellular activities. In this review, we summarize the recent progress in biomaterials with hierarchical structures for tissue regeneration. We explore the various methods and strategies employed in designing biomaterials with hierarchical structures of different dimensions. The improvement of physicochemical properties and bioactivities by hierarchically structured biomaterials, including the regulation of mechanical properties, degradability, and the specific functions of cell behaviors, has been highlighted. Furthermore, the current applications of hierarchically structured biomaterials for tissue regeneration are discussed. Finally, we conclude by summarizing the developments of hierarchically structured biomaterials for tissue regeneration and provide future perspectives.
Download or read book Osteogenic Differentiation of Human Mesenchymal Stem Cells Enhanced by Bone ECM Analogous Nanomatrix Composites written by Jeremy B. Vines and published by . This book was released on 2011 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Bone Tissue Engineering Using Human Mesenchymal Stem Cells and Polymeric Scaffolds written by Ruwan Deepal Sumanasinghe and published by . This book was released on 2006 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: Cyclic tensile strain, Microporous fibers, mesenchymal stem cell, Osteogenic differentiation, Collagen.
Download or read book In Vitro Response To Implants With Internally Roughened Micron And Nano Features written by Jennifer M. Schneider and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Spinal fusion surgery combines two or more vertebrae together to reduce discomfort by immobilizing a painful vertebral motion segment and restoring spinal stability. The insertion of the interbody fusion device serves to support the space previously occupied by an intervertebral disc and, based on the material and topography, can also facilitate new bone formation. Recent research has focused on enhancing the endplate-contacting surfaces of interbody fusion devices and has demonstrated that micro-nano topography combined with the macro-roughness of the anti-expulsion surface can enhance stem cell morphology and differentiation. However, an often overlooked feature of fusion devices is the topography of the internal walls of interbody spinal implants which may play an important role during bone graph integration and fusion. This evaluation examines whether uniform application of surface topography at a cellular level invokes a similar cyto/chemokines expression and phenotype when exposed to mesenchymal stem cells (MSCs), regardless of location on the device.The results presented in this study demonstrate equivalent hMSC gene expression and morphology on the interior and exterior surfaces of devices with uniform topography at a cellular level. Specifically, the nanoLOCKu00ae surface induced an osteoblastic phenotype in hMSCs on both surfaces, whereas the smooth titanium and PEEK demonstrated a fibroblastic morphology across both interior and exterior surfaces. The cell morphology on the nanoLOCKu00ae surface correlated with the production of osteogenic cyto and chemokines, while PEEK promoted upregulation of inflammatory cyto and chemokines.
Download or read book Polymeric Biomaterials for Tissue Regeneration written by Changyou Gao and published by Springer Nature. This book was released on 2024-02-01 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews state of the art of polymeric biomaterials for regenerative medicine and highlights advances in both fundamental science and clinical practice. It summarizes the latest techniques in polymeric scaffold fabrication, delivery carriers, physiochemical property modulation, as well as their influence on the adhesion and performance of biomolecules, cells, and tissues. It also describes methods for creating biofunctional surfaces/interfaces and subsequently modulating the host response to implantable materials. Lastly, it discusses the applications of biomaterials and constructs in soft-tissue regenerative medicine. It is a valuable resource for materials scientists and engineers wishing to identify research priorities to fulfill clinical needs and provides physicians with insights into emerging novel biomaterials. This integrated approach also offers engineering students a sense of the relevance of materials science in the development of novel therapeutic strategies. In the second edition, most of the chapters are updated according to the latest progress of this research field. A new chapter on nerve regeneration is also included.
