Download or read book Basic HPLC and CE of Biomolecules written by Robert L. Cunico and published by Bay Bioanalytical Laboratory, Incorporated. This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Academic Press. This book was released on 2000-02-16 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.

Download or read book High Performance Liquid Chromatography Capillary Electrophoresis written by Andrea Weston and published by Elsevier. This book was released on 1997-07-21 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. - Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques - Applicable to a wide variety of separation problems - Covers basic concepts governing any separation as well as instrumentation and how to use it - Helps the user to obtain optimal resolution in minimal time - Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation - Includes information on upcoming trends such as miniaturization - Major concepts in each chapter are organized to allow access to information easily and quickly - Contains practical bibliography for accessing the literature

Download or read book HPLC and CE written by Andrea Weston and published by . This book was released on 1997-01-01 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a source of information on the powerful separation techniques high performance liquid chromatography and capillary electrophoresis, this text presents the fundamental theory, instrumentation, modes of operation, and optimization of these separations. Also provided is coverage of troubleshooting techniques, improvement of resolution, data manipulation, selectivity, and sensitivity. Information is also included on special procedures such as chiral separations, affinity, chromatography, and sample preparation and current trends such as miniaturization.

Download or read book Green Analytical Chemistry written by Mihkel Koel and published by Royal Society of Chemistry. This book was released on 2015-11-09 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concerns about environmental pollution, global climate change and hazards to human health have increased dramatically. This has led to a call for change in chemical processes including those that are part of chemical analysis. The development of analytical chemistry continues and every new discovery in chemistry, physics, molecular biology, and materials science brings new opportunities and challenges. Yet, contemporary analytical chemistry does not consume resources optimally. Indeed, the usage of toxic chemical compounds is at the highest rate ever. All this makes the emerging field of green chemistry a “hot topic” in industrial, governmental laboratories as well as in academia. This book starts by introducing the twelve principles of green chemistry. It then goes on to discuss how the principles of green chemistry can be used to assess the ‘greenness’ of analytical methodologies. The ‘green profile’ proposed by the ACS Green Chemistry Institute is also presented. A chapter on “Greening” sample preparation describes approaches to minimizing toxic solvent use, using non-toxic alternatives, and saving energy. The chapter on instrumental methods describes existing analytical approaches that are inherently green and making non-green methods greener. The final chapter on signal acquisition describes how quantitative structure-property relationship (QSPR) ideas could reduce experimental work thus making analysis greener. The book concludes with a discussion of how green chemistry is both possible and necessary. Green Analytical Chemistry is aimed at managers of analytical laboratories but will also interest teachers of analytical chemistry and green public policy makers.

Download or read book Analysis of Pharmaceuticals by Capillary Electrophoresis written by Kevin D. Altria and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dieser erste Titel einer ganzen Serie von anwendungsbezogenen Handbüchern zur Kapillarelektrophorese beschäftigt sich mit der Analytik von pharmazeutischen Substanzen. Dabei werden verschiedene Techniken praxisnah erläutert. Jeder, der im Labor - ob wissenschaftlich oder praxisnah - mit der Analyse von oft chiralen Pharmazeutika konfrontiert ist, wird viele Hinweise und Tips für seine Arbeit finden.USP: Einzige Monographie zur Analyse von Pharmazeutika mit CE This book describes the current state of the art for the analysis of pharmaceuticals by capillary electrophoresis and contains several hundred references to specific applications and methods. The main purpose of the book is to present the application possibilities of CE an therefore tabulated application data are provided. Chapters of the book are devoted to providing details of individual application areas such as chiral analysis, determination of drug related impurities, determination of drug counter-ions, drug residue monitoring and main component assay. An introductory chapter provides theoretical background to CE an related techniques. A chapter is dedicated to capillary electrochromatography which highlights the importance this technique currently possesses. Successful regulatory acceptance of CE methods is also described. A comprehensive chapter covers method validation aspects. Other chapters include discrete areas such as the use of non-aqueous solvents, forensic applications of CE, the application of experimental designs, determination of drugs in biofluids, and the analysis of vitamins by CE.

Download or read book Chromatography and Capillary Electrophoresis in Food Analysis written by Hilmer Sorensen and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the rapidly developing field of analysis it is important to be aware of the newest methods within available techniques. Chromatography and Capillary Electrophoresis in Food Analysis describes chromatographic and electrophoretic principles and procedures for analyses of various amphiphilic and hydrophilic biomolecules, particularly for food analysis. Providing basic information, including general sample preparation, the book then goes on to describe individual analytical methods and exemplify the strategy and methodologies employed for the analyses. The theory necessary to understand the methods and interpretation of results is also included, as are numerous detailed instructions on experiments. Tables, figures and references are included to give a complete picture. Chromatography and Capillary Electrophoresis in Food Analysis will be especially valuable for students and more experienced researchers interested in analysis of natural products, both inside and outside the field of food chemistry.

Download or read book HPLC in Enzymatic Analysis written by Edward F. Rossomando and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Download or read book Capillary Electrophoresis for Food Analysis written by Richard A Frazier and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its inception in the early 1980s, capillary electrophoresis (CE) offers a great deal of flexibility as a modern analytical technique, and has found applications within many fields of analysis, particularly pharmaceutical science and biochemistry. Until now, food analysts have had difficulties in adopting the technique due to the lack of written guidance. Capillary Electrophoresis for Food Analysis: Method Development provides basic information and the support needed to enable food analysts to utilise the technique for the development of new separation methods. Designed specifically for the needs of food analysts, the book takes the reader step by step through the process of developing and troubleshooting CE methods. Worked examples are included to make it ideal as a laboratory companion as well as a library reference source.

Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Download or read book The Analysis of Biological Samples Using Capillary Electrophoresis and HPLC Technologies written by and published by . This book was released on 2007 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) are two very important separation techniques. CE is a microseparation technique in which an electric field is applied to perform the separation. In CE, charged species are separated based on their charge/mass ratio, making this technique useful for separating a wide variety of components, including those found in biological samples. HPLC is the most widely used separation technique due to the ease of use and wide variety of components that can be separated. HPLC employs the use of pressure to push mobile phase through an analytical column containing a stationary phase that interacts with the solutes to effect the separation. Both CE and HPLC can use a number of different detection schemes to monitor the separated species, including ultra-violet (UV), laser-induced fluorescence (LIF), and mass spectrometry (MS). Affinity capillary electrophoresis (ACE) combines the separation power of CE with the selectivity of immunoassays. A fluorescein labeled estradiol derivative was evaluated for potential use as a competitive inhibitor in ACE. The labeling procedure rendered multiple components with the fluorescein label. Two of the components were identified as active estradiol species against the anti-estradiol antibody, examined by CE-LIF. Since only one active species had been predicted, both species were characterized using CE-MS and MS/MS, in addition to testing them in the ACE format. Nucleoside reverse transcriptase inhibitors (NRTIs) are one class of anti-HIV (human immunodeficiency virus) drugs. Because HIV is considered a pandemic, the analysis of NRTIs in human samples is very important; people all over the world are using these drugs. Currently, multiple NRTIs are given for treatment; however, there are few methods to date that separate and detect multiple NRTIs in a single run. NRTIs are metabolized in the body into their active triphosphate form. Two approaches can be followed for their separation and detection. The first approach was an indirect method, in which the active triphosphate form was dephosphorylated into the parent form of the NRTIs. HPLC with UV detection was utilized to establish an optimized separation method prior to using LC-MS/MS for the analysis of the following NRTIs in human peripheral blood monunclear cells (PBMCs): stavudine (D4T), lamivudine (3TC), the metabolized form of didanosine (ddA), zidovudine (ZDV), and the internal standard. The second approach was a direct method, in which analysis of the triphosphate form of the NRTIs was performed without dephosphorylation of the drugs. A capillary eletrophoretic method was developed that allows separation of the triphosphate NRTIs of interest: zidovudine triphosphate (ZDV-TP), stavudine triphosphate (D4T-TP), lamivudine triphosphate (3TC-TP), 2',3'-dideoxyadenoine triphosphate (ddA-TP), tenofivir diphosphate (TFV-DP), and emtricitabine triphosphate (FTC-TP). The separation was optimized using UV detection. Since UV detection does not offer appropriate detection limits, we studied the native fluorescence spectroscopic characteristics of the triphosphate NRTIs. We constructed an in-house CE-LIF system to explore LIF detection of the phosphorylated NRTIs. The system employed the use of a deep-UV laser with excitation at 224 nm. A sheath-flow cuvette was integrated into the design to improve detectability. The CE-LIF system was aligned and tested using the native fluorescence of tryptophan. The CE-LIF system was then used for the analysis of the active metabolized forms of NRTIs. With this system, we were able to separate and detect ZDV-TP, D4T-TP, 3TC-TP, ddA-TP, and the internal standard using a modification of the separation conditions previously optimized with UV detection. The detection limits obtained are not sufficiently low for the analysis of human PBMCs.

Download or read book Capillary Electrophoresis of Proteins written by Tim Wehr and published by CRC Press. This book was released on 1998-10-20 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Download or read book Handbook of Capillary Electrophoresis Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Download or read book Capillary Electrophoresis written by Dale R. Baker and published by Wiley-Interscience. This book was released on 1995-03-02 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting. Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest.

Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.

Download or read book Capillary Electrochromatography written by Keith D Bartle and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrochromatography (CEC) is a new and exciting hybrid separation technique that seeks to exploit the combined advantages of both capillary electrophoresis (high efficiencies) and HPLC (mobile and stationary phase selectivity). It is a technique with tremendous potential, especially in the pharmaceutical and biomedical fields. This is the first book to be devoted to the topic and presents reviews by the world leaders in the field on the theory and development of the technique and current and potential future applications. Capillary Electrochromatography provides an excellent introduction to the field for graduates and professionals in industry and academia with an interest in separation science.

Download or read book Separation Methods in Drug Synthesis and Purification written by Klara Valko and published by Elsevier. This book was released on 2000-10-13 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation Methods in Drug Synthesis and Purification