Download or read book Metabolic Engineering written by Sang Yup Lee and published by John Wiley & Sons. This book was released on 2021-06-02 with total page 1075 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Download or read book Mass Spectrometry written by Edmond de Hoffmann and published by Wiley. This book was released on 2001-10-10 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a complete overview of the principles, theories and key applications of modern mass spectrometry in this introductory textbook. Following on from the highly successful first edition, this edition is extensively updated including new techniques and applications. All instrumental aspects of mass spectrometry are clearly and concisely described; sources, analysers and detectors. * Revised and updated * Numerous examples and illustrations are combined with a series of exercises to help encourage student understanding * Includes biological applications, which have been significantly expanded and updated * Also includes coverage of ESI and MALDI

Download or read book Metabolic Analysis Using Stable Isotopes written by and published by Academic Press. This book was released on 2015-09-21 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes. - Continues the legacy of this premier serial with quality chapters on metabolic analysis using stable isotopes - Represents the newest volume in Methods in Enzymology, providing a premier serial with quality chapters authored by leaders in the field - Ideal reference for those interested in the study of metabolism, metabolic tracing, isotopic labeling, and lipogenesis

Download or read book The Handbook of Plant Metabolomics written by Wolfram Weckwerth and published by John Wiley & Sons. This book was released on 2013-02-28 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series this new book presents an excellent overview of different approaches and techniques in Metabolomics. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, the title meets a growing market. The Kahl books are in addition a trusted brand for the plant science community and have always sold above expectations.

Download or read book High Throughput Metabolomics written by Angelo D'Alessandro and published by Humana. This book was released on 2020-06-06 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.

Download or read book Lipidomics written by Xianlin Han and published by John Wiley & Sons. This book was released on 2016-05-02 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the area of lipidomics from fundamentals and theory to applications Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes

Download or read book Amino Acid Analysis written by Michail A. Alterman and published by Humana Press. This book was released on 2011-12-02 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Download or read book Metabolomics written by Wolfram Weckwerth and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Download or read book Isotope Tracers in Metabolic Research written by Robert R. Wolfe and published by John Wiley & Sons. This book was released on 2004-10-18 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased. New analytical tools at the genetic and protein levels have spurred this growth, opening the door for a deeper understanding of metabolic events. This in turn promises to yield significant advances in the understanding and treatment of human disease. Now fully revised and expanded, Isotope Tracers in Metabolic Research, Second Edition is the established definitive text on stable and radioactive isotope tracers. In unique, multidisciplinary fashion, it presents comprehensive coverage of new methodological, mathematical, and theoretical approaches. This new Second Edition includes: All-new chapters on nuclear magnetic resonance, mass isotopomer analysis, and methods of protein metabolism analysis A completely updated categorized list of over 750 references Major advances in the development of mass isotopomer and positional isotopomer techniques, noninvasive isotope techniques for studying metabolic pathways, hyphenated techniques, and new tracer techniques The latest developments in quantification of DNA synthesis and mass spectrometry spurred by genome sequencing and proteomics New coverage of mathematical modeling Expanded coverage of microdialysis probes, laboratory procedures, and regulatory issues related to human studies In this complete guide to performing tracer studies, the authors systematically cover tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and data interpretation. Problems and discussion questions highlight key points in each chapter. Isotope Tracers in Metabolic Research, Second Edition offers students and researchers a comprehensive, practical resource for utilizing the latest tracer methodologies.

Download or read book The Metabolic Pathway Engineering Handbook written by Christina Smolke and published by CRC Press. This book was released on 2009-07-28 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction

Download or read book Metabolome Analysis written by Silas G. Villas-Boas and published by John Wiley & Sons. This book was released on 2007-02-16 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and secondary metabolites. Includes four chapters discussing successful metabolome studies of different organisms. Highlights the analytical challenges of studying metabolites. Illustrates applications of metabolome analysis through the use of case studies.

Download or read book Stable Isotope Ecology written by Brian Fry and published by Springer Science & Business Media. This book was released on 2007-01-15 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.

Download or read book Cancer Signaling written by Christoph Wagener and published by John Wiley & Sons. This book was released on 2016-08-12 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer, which has become the second-most prevalent health issue globally, is essentially a malfunction of cell signaling. Understanding how the intricate signaling networks of cells and tissues allow cancer to thrive - and how they can be turned into potent weapons against it - is the key to managing cancer in the clinic and improving the outcome of cancer therapies. In their ground-breaking textbook, the authors provide a compelling story of how cancer works on the molecular level, and how targeted therapies using kinase inhibitors and other modulators of signaling pathways can contain and eventually cure it. The first part of the book gives an introduction into the cell and molecular biology of cancer, focusing on the key mechanisms of cancer formation. The second part of the book introduces the main signaling transduction mechanisms responsible for carcinogenesis and compares their function in healthy versus cancer cells. In contrast to the complexity of its topic, the text is easy to read. 32 specially prepared teaching videos on key concepts and pathways in cancer signaling are available online for users of the print edition and have been integrated into the text in the enhanced e-book edition.

Download or read book Using Geochemical Data written by Hugh Rollinson and published by Cambridge University Press. This book was released on 2021-05-06 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook is a complete rewrite, and expansion of Hugh Rollinson's highly successful 1993 book Using Geochemical Data: Evaluation, Presentation, Interpretation. Rollinson and Pease's new book covers the explosion in geochemical thinking over the past three decades, as new instruments and techniques have come online. It provides a comprehensive overview of how modern geochemical data are used in the understanding of geological and petrological processes. It covers major element, trace element, and radiogenic and stable isotope geochemistry. It explains the potential of many geochemical techniques, provides examples of their application, and emphasizes how to interpret the resulting data. Additional topics covered include the critical statistical analysis of geochemical data, current geochemical techniques, effective display of geochemical data, and the application of data in problem solving and identifying petrogenetic processes within a geological context. It will be invaluable for all graduate students, researchers, and professionals using geochemical techniques.

Download or read book Systems Metabolic Engineering written by Christoph Wittmann and published by Springer Science & Business Media. This book was released on 2012-06-15 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Download or read book Plant Metabolites and Regulation under Environmental Stress written by Parvaiz Ahmad and published by Academic Press. This book was released on 2018-03-19 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Metabolites and Regulation Under Environmental Stress presents the latest research on both primary and secondary metabolites. The book sheds light on the metabolic pathways of primary and secondary metabolites, the role of these metabolites in plants, and the environmental impact on the regulation of these metabolites. Users will find a comprehensive, practical reference that aids researchers in their understanding of the role of plant metabolites in stress tolerance. - Highlights new advances in the understanding of plant metabolism - Features 17 protocols and methods for analysis of important plant secondary metabolites - Includes sections on environmental adaptations and plant metabolites, plant metabolites and breeding, plant microbiome and metabolites, and plant metabolism under non-stress conditions