Download or read book High Performance Liquid Chromatography with Tandem Laser Induced Fluorescence and Mass Spectrometry Detection Methods to Monitor the Metabolic Profile of Doxorubicin and Its Alteration with Age written by Joseph Bradly Katzenmeyer and published by . This book was released on 2010 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High Performance Liquid Chromatography written by Mark Cletus Roach and published by . This book was released on 1986 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ultra Performance Liquid Chromatography Mass Spectrometry written by Mu Naushad and published by CRC Press. This book was released on 2014-03-18 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a unique collection of up-to-date UPLC-MS/MS (ultra performance liquid chromatography-tandem mass spectrometric) methods for the separation and quantitative determination of pesticides, capsaicinoids, heterocyclic amines, aflatoxin, perfluorochemicals, acrylamide, procyanidins and alkaloids, lactose content, phenolic compounds, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.
Download or read book Therapeutic Drug Monitoring and Toxicology by Liquid Chromatography written by Steven H.Y. Wong and published by CRC Press. This book was released on 1985-02-01 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: This important, new reference provides the first , up-to-date, and comprehensive treatmentof the various liquid chromatography (LC) instrumentation for Therapeutic DrugMonitoring (TDM) and toxicology drug assays and reviews the clinical pharmacologyof major classes of drugs and their LC analyses.Written by authoritative contributors who bring many years of personal laboratory/clinical experience to the book, this interdisciplinary work : presents the principlesof TDM, sampling techniques, and various instrumentation topics, including computerinterfacing, mass spectrometry, fluorescence , electrochemical detection, and bloodcollection devices . .. examines six primary classes of drugs, complete with recommendedLC procedures and emphasizing recently introduced drugs and their LC analyses. .. and documents medicolegal guidelines and laboratory management considerations,providing a contemporary assessment of today's laboratory needs.The only practical, current guide available on the subject, Therapeutic Drug Monitoringand Toxicology by Li([uid Chromatography belongs in the personal libraries of clinicaland analytical chemists and biochemists, clinical pharmacologists, clinical toxicologists,clinical pathologists, immunologists, liquid chromatographers, mass spectroscopists, laboratorydirectors, laboratory instrument manufacturers , and medical technologists. Thebook is also vital supplementary reading for advanced undergraduate and graduate-levelclinical chemistry, toxicology, clinical pathology, and liquid chromatography course.
Download or read book Using Mass Spectrometry for Drug Metabolism Studies written by Walter A. Korfmacher and published by CRC Press. This book was released on 2004-12-17 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) is fast becoming the premier tool for analyzing various drug metabolism samples in the early phases of drug discovery and research. Introducing the newer, more powerful MS equipment and exploring new applications for using them, this book provides a state-of-the-art look at this promising field. Using Mass Spectrometry
Download or read book Development of Metabolomics and Lipidomics Workflows for Phosphorylated Metabolites Using Ultra high performance Liquid Chromatography with Tandem Mass Spectrometry written by Peng Li and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decades, system biology and multi-omics have become an emerging field in life science. With the development of analytical instruments, especially the mass spectrometry (MS), MS based metabolomics are widely utilized to understanding the metabolic status. Due to significantly different properties of lipids, lipidomics became more and more an independent research discipline. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is the most popular analytical platform in metabolomics and lipidomics. Recent progress in bioinformatical approaches has also facilitated the bottlenecking data processing steps. Although some widely used methods were established for general metabolomics and lipidomics analysis, there are still some challenging analytes like phosphorylated metabolites/lipids, which showed compromised performance. Through highly regulated phosphorylation and dephosphorylation by enzymes, phosphorylated metabolites/lipids regulate many important cellular processes. Despite their importance, the performance of their current analytical methods is not satisfying. Generally, analysis of phosphorylated analytes by LC-MS/MS face the following challenges, including low abundance, low extraction recovery, instability, regioisomers, availability of standards, etc. In order to address these issues, we developed new methods based on phosphate methylation. Accordingly, complete workflows from sample preparation to data processing were developed and validated in biological matrix. In the 1st project, a targeted LC-MS/MS method of fatty acyl-CoAs (acyl-CoAs) with different chain lengths was established. Acyl-CoAs are central metabolic intermediates in numerous biological processes. Due to diverse fatty acyl compositions, acyl-CoAs vary greatly in polarity, which poses great challenges to their chromatographic separation. Currently, short chain acyl-CoAs are separated by HILIC or acidic RP-LC, while medium-, long- and very long-chain acyl-CoAs are analysed in basic RP-LC. Additionally, carryover in LC-MS/MS instrumentation requires extra flushing/cleaning procedures. To address these problems, we developed phosphate methylation strategy for acyl-CoAs. In the sample preparation, mixed-mode SPE was optimized to be compatible with methylation. SRM transitions in MS detection was constructed with fragmentation rule for all methylated acyl-CoAs. Uniformly 13C labelled yeast extract was used as internal standards for accurate quantification. Odd chain and stable isotope labelled analogs were used as surrogate calibrators. The LOQs were between 16.9 nM and 4.2 nM. The method was applied in cultured HeLa cells and human platelets of coronary artery disease patients. The results showed that the established method can be used to profile acyl-CoAs in biological samples. In the 2nd project, an isomer selective LC-MS/MS method of PIPx was developed. PIPx is a family of low abundant phospholipids commonly found in eukaryotic cells. Starting from phosphoinositol (PI), PIPx family consists of three lipid classes (PIP, PIP2, and PIP3) according to phosphorylation degree. There are three distinct regioisomers in each class of PIP and PIP2. As phospholipids, PIPx has two fatty acyl groups at the sn1 and sn2 position of a glycerol backbone. Currently, methods capable of regioisomer separation and fatty acyl coverage are still missing. To develop a comprehensive method, we utilized polysaccharide chiral column with data independent acquisition technique. Stable isotope labelled PIPx were characterized and used as internal standards. The established method was applied to several biological samples, including NIST SRM1950 plasma, Pichia Pastoris, and cultured HeLa cells. The results indicate this method could effectively monitor PIPx profiles in real samples and facilitate our understanding of physiological and pathological conditions. In the 3rd and 4th project, improved LC-MS/MS methods of IPx and SPx were developed. Compared to PIPx and acyl-CoAs, IPx and SPx are more polar and hydrophilic. To apply the phosphate methylation strategy, a new derivatization solvent mixture was developed and optimized. To improve extraction recovery, an extraction kit compromised of TiO2 and centrifuge filter was established. Differential isotope labelling methylation was utilized to generate stable isotope labelled analogs in both projects (internal standards in IPx project, surrogate calibrators in SPx project). Cholesterol-ether bonded RP column was used to separate IPx regioisomers, especially I(1,4,5)P3. Porous graphite carbon column was used to separate SPx in glycolysis pathway. After validation in biological matrix, the methods were applied in different biological samples, including NIST SRM1950 plasma, cultured HeLa cells, and human platelets.
Download or read book Liquid Chromatography Mass Spectrometry written by Robert E. Ardrey and published by Wiley. This book was released on 2003-04-01 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: First explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique. * First book to concentrate on principles of LC-MS * Explains principles of mass spectrometry and chromatography before moving on to LC-MS * Describes instrumental aspects of LC-MS * Discusses current applications of LC-MS and shows benefits of using this technique in practice
Download or read book Ultra Performance Liquid Chromatography Mass Spectrometry written by Taylor & Francis Group and published by CRC Press. This book was released on 2021-12-13 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a unique collection of up-to-date UPLC-MS/MS (ultra performance liquid chromatography-tandem mass spectrometric) methods for the separation and quantitative determination of pesticides, capsaicinoids, heterocyclic amines, aflatoxin, perfluorochemicals, acrylamide, procyanidins and alkaloids, lactose content, phenolic compounds
Download or read book High Performance Liquid Chromatography and Mass Spectrometry written by William Kerr and published by . This book was released on 2019-08-23 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: High-performance liquid chromatography (HPLC), also known as high-pressure liquid chromatography, is an advanced type of LC. HPLC is amenable to a wide range of applications, such as pharmaceuticals and food analysis. It is especially useful for low or non-volatile organic compounds, which cannot be handled with gas chromatography. Liquid chromatography-mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). Coupled chromatography - MS systems are popular in chemical analysis because the individual capabilities of each technique are enhanced synergistically. While liquid chromatography separates mixtures with multiple components, mass spectrometry provides structural identity of the individual components with high molecular specificity and detection sensitivity. This tandem technique can be used to analyze biochemical, organic, and inorganic compounds commonly found in complex samples of environmental and biological origin. Therefore, LC-MS may be applied in a wide range of sectors including biotechnology, environment monitoring, food processing, and pharmaceutical, agrochemical, and cosmetic industries. Mass spectrometry (MS) is an analytical technique that measures the mass-to-charge ratio (m/z) of charged particles (ions). Although there are many different kinds of mass spectrometers, all of them make use of electric or magnetic fields to manipulate the motion of ions produced from an analyze of interest and determine their m/z. The book has primarily been designed for analysts who are unfamiliar with this technique or have limited training and experience in this or have constraints in finding a suitable HPLC method. The book presents a comprehensive compilation of reviews, opinions and applications of the use of Mass Spectrometry to analyze biomolecules.
Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.
Download or read book Application of Liquid Chromatography tandem Mass Spectrometry Techniques to the Study of Two Biological Systems written by Mary Elizabeth Eisenhauer and published by . This book was released on 2011 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quorum sensing is a type of bacterial cell-cell communication that uses diffusible signaling molecules to allow the regulation of gene expression based on cell density. The two types of signaling molecules discussed here are autoinducer-2 (AI-2) and a class of signaling molecules of the N-acylhomoserine lactone type (AHL) also known as autoinducer-1 (AI-1). The biosynthesis of both of these molecules has origins in a metabolic pathway. Although undisputed in some cases, the role of AI-2 and AHLs in bacterial systems has come into question. Here, the functionalities of these molecules were investigated by utilizing isotope-labeled versions of both AI-2 and AHLs in conjunction with liquid chromatography tandem mass spectrometry in order to quantify the natural abundance of these molecules in various bacterial cultures. Presented in this thesis are, a synthesis for doubly-deuterated AHLs as well as chromatographic and spectrometric methods for the detection and quantitation of these molecules. Additionally, a series of relevant biological studies which effectively and prolifically utilize these synthetic and analytical techniques are presented here. The selective destruction of beta-cell mass in the Langerhans of the pancreas is known to cause Type 1 diabetes mellitus (T1DM). Currently, many key aspects of this autoimmune disease remain unclear, including the exact mechanism of beta-cell death. In a collaborative project with Dr. J. Jason Collier, we sought to test the hypothesis that different mechanisms of cell death will present discrete phenotypic profiles which can be distinguished by a specific metabolic response in response to the appropriate stimuli. A second project presented in this thesis is the development and implementation of a method to profile the metabolic signatures of two types of pancreatic beta-cell death using tandem mass spectrometry techniques. Using 832/13 rat insulinoma cells, the metabolite pools of cells exposed to either pro-inflammatory cytokines or known apoptosis inducers, such as camptothecin, were analyzed. In this investigation, it was found that this method was effective in defining reproducible metabolic differences in each sample tested. Taken together with complementary methods used in the Collier lab, the results collectively demonstrate that pancreatic beta-cells undergo apoptosis in response to camptothecin, but not pro-inflammatory cytokines.
Download or read book Measuring Oxidants and Oxidative Stress in Biological Systems written by Lawrence J. Berliner and published by Springer Nature. This book was released on 2020-08-08 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.
Download or read book Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques written by Paul H. Gamache and published by John Wiley & Sons. This book was released on 2017-05-30 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book devoted exclusively to a highly popular, relatively new detection technique Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques presents a comprehensive review of CAD theory, describes its advantages and limitations, and offers extremely well-informed recommendations for its practical use. Using numerous real-world examples based on contributors’ professional experiences, it provides priceless insights into the actual and potential applications of CAD across a wide range of industries. Charged aerosol detection can be combined with a variety of separation techniques and in numerous configurations. While it has been widely adapted for an array of industrial and research applications with great success, it is still a relatively new technique, and its fundamental performance characteristics are not yet fully understood. This book is intended as a tool for scientists seeking to identify the most effective and efficient uses of charged aerosol detection for a given application. Moving naturally from basic to advanced topics, the author relates fundamental principles, practical uses, and applications across a range of industrial settings, including pharmaceuticals, petrochemicals, biotech, and more. Offers timely, authoritative coverage of the theory, experimental techniques, and end-user applications of charged aerosol detection Includes contributions from experts from various fields of applications who explore CAD’s advantages over traditional HPLC techniques, as well its limitations Provides a current theoretical and practical understanding of CAD, derived from authorities on aerosol technology and separation sciences Features numerous real-world examples that help relate fundamental properties and general operational variables of CAD to its performance in a variety of conditions Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques is a valuable resource for scientists who use chromatographic techniques in academic research and across an array of industrial settings, including the biopharmaceutical, biotechnology, biofuel, chemical, environmental, and food and beverage industries, among others.
Download or read book Electrospray and MALDI Mass Spectrometry written by Richard B. Cole and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Download or read book Handbook of Drug Monitoring Methods written by Amitava Dasgupta and published by Springer Science & Business Media. This book was released on 2007-10-23 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written in a handbook style with specific methods and tips on eliminating false positive and false negative results, this book is a practical guide to the detailed mechanisms of such occurrences.
Download or read book Redox Proteomics written by Isabella Dalle-Donne and published by John Wiley & Sons. This book was released on 2006-08-11 with total page 978 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methodology and applications of redox proteomics The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease, assess risks, determine prognoses, and target therapeutic strategies for people with inflammatory and aging-associated diseases. This collection brings together, in one comprehensive volume, a broad array of information and insights into normal and altered physiology, molecular mechanisms of disease states, and new applications of the rapidly evolving techniques of proteomics. Written by some of the finest investigators in this area, Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases examines the key topics of redox proteomics and redox control of cellular function, including: * The role of oxidized proteins in various disorders * Pioneering studies on the development of redox proteomics * Analytical methodologies for identification and structural characterization of proteins affected by oxidative/nitrosative modifications * The response and regulation of protein oxidation in different cell types * The pathological implications of protein oxidation for conditions, including asthma, cardiovascular disease, diabetes, preeclampsia, and Alzheimer's disease Distinguished by its in-depth discussions, balanced methodological approach, and emphasis on medical applications and diagnosis development, Redox Proteomics is a rich resource for all professionals with an interest in proteomics, cellular physiology and its alterations in disease states, and related fields.
Download or read book Personalized Medicine in Oncology written by Ari VanderWalde and published by . This book was released on 2022 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nowhere is the explosion in comprehensive genomic testing more evident than in oncology. Multiple consensus guidelines now recommend molecular testing as the standard of care for most metastatic tumors. To aid in the advancement of this rapidly changing field, we intend this Special Issue of JPM to focus on technical developments in the genomic profiling of cancer, detail promising somatic alterations that either are, or have a high likelihood of being, relevant in the near future, and to address issues related to the pricing and value of these tests.The last few years have seen the cost of molecular testing decrease by orders of magnitude. In 2018, we saw the first “site-agnostic” drug approvals in cancer (for microsatellite unstable cancer (PD-1 inhibitors) and NTRK-fusions (TRK inhibitors)). Research on targetable mutations, determination of genetic “signatures” that can use multiple individual genes/pathways, development of targeted therapy, and insight into the value of new technology remains at the cutting edge of research in this field. We are soliciting papers that present new technologies to assess predictive biomarkers in cancer, original research (pre-clinical or clinical) that demonstrates promise for particular targeted therapies in cancer, and articles that explore the clinical and financial impacts of this paradigmatic shift in cancer diagnostics and treatment.
