Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.

Download or read book Isolation of Host Proteins Involved in Herpes Simplex Virus Type 1 DNA Synthesis written by Whitney Cogswell and published by . This book was released on 2008 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Herpes simplex virus type 1 (HSV-1) is the prototypic member of a large family of viruses known as Herpesviridae. Herpesviruses possess a double-stranded DNA genome and infect a broad range of species. In addition to HSV-1, there are seven other herpesviruses that are known to infect humans. Although antiviral treatment is available for HSV infections, there are few or no options for treatment of infections by other herpesviruses. One of the best targets for development of new antivirals is the viral DNA replication apparatus. HSV-1 encodes for seven proteins directly involved in viral DNA replication. However, no HSV-1 origin-dependent DNA replication has been reconstituted in vitro using purified viral proteins. It is hypothesized that proteins (host and/or viral) in addition to the seven known viral DNA replication proteins are involved in viral DNA replication in vivo. Moreover, it is predicted that such proteins would interact with one or more of the viral DNA replication proteins. In an effort to identify interacting host proteins, co-immunoprecipitation analysis was performed using antibodies specific for an essential HSV DNA replication protein, UL42, the polymerase processivity factor. Extracts from infected and mock-infected cells were immunoprecipitated with the UL42 antibody. UL42, and all that is bound to it, bind to the antibody which binds to the Protein G agarose matrix. Those proteins that are specifically pulled down from infected cell extracts, but not from mock-infected extracts, were to be identified by mass-spectrometry. The immunoprecipitates were analyzed following separation on denaturing polyacrylamide gels. Gels were silver-stained or analyzed for the presence of UL42 using a Western blot. Conditions were adjusted to optimize the amount of UL42 immunoprecipitated, but UL42 could not be immunoprecipitated quantitatively. Following immunoprecipitation under optimum conditions, no differences were observed between the proteins pulled-down from mock- or infected-cell extracts. Due to the poor ability of the antibody to pull-down UL42, a second approach was employed. A histidine-tagged version of UL42 was purified using a nickel chelating column and then mixed with infected or mock-infected cell extracts. Those proteins that specifically interact with his-UL42 were expected to bind to a nickel column. However, nonspecific interactions with the matrix were observed and prevented identification of proteins by mass-spectrometry.

Download or read book Herpes Simplex Virus DNA Synthesis written by Claes Gustafsson and published by . This book was released on 1995 with total page 57 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Herpes Simplex Virus 1 DNA Packaging written by Ying Fan and published by . This book was released on 1997 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on Herpes Simplex Virus DNA Synthesis written by Frazer John Rixon and published by . This book was released on 1977 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book DNA Replication in Eukaryotic Cells written by Melvin L. DePamphilis and published by CSHL Press. This book was released on 1996 with total page 1080 pages. Available in PDF, EPUB and Kindle. Book excerpt: National Institutes of Health. Cold Spring Harbor Monograph, Volume 31 Extensive text on the replication of DNA, specifically in eukaryotic cells, for researchers. 68 contributors, 54 U.S.

Download or read book Herpesvirus DNA written by Yechiel Becker and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpesviruses, classified in the family Herpesviridae, are important human and animal pathogens that can cause primary, latent or recurrent infections and even cancer. The major interest in research on herpesviruses today focuses on understanding the organization of the DNA genome, as well as on characterizing the viral genes in regard to their control and function. Modern techniques have allowed the viral DNA to become a molecular tool in the study of gene function, since it is now possible to implant the DNA into eukaryotic cells. This book contains original studies on the structure and organization of the DNA of human and animal herpes viruses. The various chapters acquaint the reader with the organization of the viral DNA, the mRNA transcripts, the replicative intermediates of the viral DNA, defective DNA genomes and their mode of synthesis, analyses of the viral DNA sequences in transformed cells, and the relationship between the presence of viral DNA fragments in the cancer cells and the transformed state of the cells.

Download or read book From the Hallowed Halls of Herpesvirology written by John A. Blaho and published by World Scientific. This book was released on 2012 with total page 726 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains a series of review chapters from the world''s leaders in herpesvirus research. It is designed as a tribute to the famous virologist Prof Bernard Roizman. Former trainees of Prof Roizman were invited to contribute to this volume. The chapters cover all eight of the human herpesviruses, and the topics discussed span the past six decades of this exciting research field.

Download or read book Herpes Simplex Virus New Insights for the Healthcare Professional 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Herpes Simplex Virus. The editors have built Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Herpes Simplex Virus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book The Role of the 3 5 Exonuclease Activity of Herpes Simplex Virus Type 1 in Translesion DNA Synthesis written by Jason Stroud and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Accurate copying of DNA is vital for maintaining genome integrity and organismal viability. The associated 3' to 5' exonuclease (exo) activity of herpes simplex virus (HSV) DNA polymerase (pol) has been shown to play multiple roles in DNA replication error avoidance. The pol-associated exo activity has been shown previously to prevent nascent strand extension in the presence of damaged primer terminus by causing futile cycles of incorporation and excision of nucleotides at the damage site. To further characterize the interaction between the exo and pol domains of the HSV pol catalytic subunit, the ability of exo-deficient (exo- ) and wild-type (wt) pol to extend a DNA primer on a template containing an apyrimidinic (AP) site was assayed. We hypothesized that the communication between the exo and pol domains would allow the enzyme to "look back" to detect an error even after it has replicated through it.

Download or read book The Role of the Associated 3 to 5 Exonuclease Activity and Processivity Factor UL42 of Herpes Simplex Virus Type 1 DNA Polymerase on the Fidelity of DNA Replication written by Liping Song and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Herpes simplex virus type 1 (HSV-1) DNA polymerase (pol) has an associated 32 to 52 exonuclease (exo) activity that plays an important role in maintaining the fidelity of DNA replication and viability of the virus. To assess the functional significance of the exo activity, the aspects of fidelity and translesion DNA synthesis affected by HSV-1 pol deficient in this exo activity (D368A) were examined and compared with those of wild-type pol. Because HSV-1 pol forms a stable heterodimer with UL42, a protein that stimulates the polymerizing activity and increases the processivity of pol, the impact of UL42 on fidelity was also evaluated. Pre-steady-state kinetic analysis revealed that the D368A mutation destroyed exo activity without significantly altering the inherent polymerization function. It was demonstrated that both wild-type and mutant pols could selectively incorporate correct vs. incorrect nucleotides, and exo activity enhanced nucleotide selectivity by roughly an order of magnitude under steady-state conditions. However, the primary effect of exo activity on fidelity was manifested during processive DNA synthesis, because it functioned to remove incorporated mismatches, the latter which impeded the extension ability of pol, particularly in the presence of UL42 processivity factor. UL42 did not alter the pre-steady-state rate constants for correct nucleotide incorporation, misincorporation, translocation, or nucleotide excision. However, UL42 decreased dissociation of pol from DNA, which not only made the enzyme more processive but also allowed enough time to excise replication errors. Lesions, such as abasic (AP) sites, also impeded extension beyond the sites, although both HSV-1 wild-type and exo-deficient pols could incorporate dATP opposite the AP site with reduced efficiencies compared to that for normal DNA synthesis. The exo activity facilitated sensing of the lesion through increased excision rate, thereby preventing extension through repeated incorporation and excision events. Taken together, the results of this study suggest that the associated exo activity of HSV-1 pol coordinates with the polymerizing activity by partitioning the primer terminus between the exo and polymerizing sites to proofread errors and prevent translesion synthesis, ultimately increasing the fidelity of HSV-1 DNA synthesis.

Download or read book Holland Frei Cancer Medicine written by Robert C. Bast, Jr. and published by John Wiley & Sons. This book was released on 2017-03-10 with total page 2004 pages. Available in PDF, EPUB and Kindle. Book excerpt: Holland-Frei Cancer Medicine, Ninth Edition, offers a balanced view of the most current knowledge of cancer science and clinical oncology practice. This all-new edition is the consummate reference source for medical oncologists, radiation oncologists, internists, surgical oncologists, and others who treat cancer patients. A translational perspective throughout, integrating cancer biology with cancer management providing an in depth understanding of the disease An emphasis on multidisciplinary, research-driven patient care to improve outcomes and optimal use of all appropriate therapies Cutting-edge coverage of personalized cancer care, including molecular diagnostics and therapeutics Concise, readable, clinically relevant text with algorithms, guidelines and insight into the use of both conventional and novel drugs Includes free access to the Wiley Digital Edition providing search across the book, the full reference list with web links, illustrations and photographs, and post-publication updates

Download or read book The Herpesviruses written by Albert Kaplan and published by Elsevier. This book was released on 1973-01-01 with total page 757 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Herpesviruses provides information pertinent to all the herpesviruses, with emphasis on the classification, morphology, replication, physical–chemical properties, and immunological relationships of all the herpesviruses. This book presents the fundamental and clinical aspects of the viruses. Organized into 21 chapters, this book starts with an overview of the classification of the herpesvirus and proceeds to explore the origins and phylogeny of the herpesviruses. This text then examines the earliest electron microscopic studies on the morphology of the herpesviruses by using shadowcast preparations of herpes simplex virus and of herpes zoster virus. Other chapters consider the serological tests as well as the antigenic relationships among herpesviruses. The final chapter deals with the clinical application of antiviral drug treatment. This book is a valuable resource for virologists, molecular biologists, veterinarians, physicians, as well as teachers and graduate students who are interested in the herpesviruses from either a fundamental or clinical viewpoint.

Download or read book The Human Herpesviruses written by Bernard Roizman and published by Raven Press (ID). This book was released on 1993 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Viral Genome Replication written by Craig E. Cameron and published by Springer Science & Business Media. This book was released on 2009-05-28 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the first comprehensive review of viral genome replication strategies, emphasizing not only pathways and regulation but also the structure-function, mechanism, and inhibition of proteins and enzymes required for this process.

Download or read book Molecular Diagnostics written by Wayne W. Grody and published by Academic Press. This book was released on 2009-11-06 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. Molecular Diagnostics provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. - Serves as the definitivereference for molecular pathologists worldwide - Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics - Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science

Download or read book Pulmonary Pathology E Book written by Dani S. Zander and published by Elsevier Health Sciences. This book was released on 2008-07-02 with total page 853 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Foundations in Diagnostic Pathology Series packs today's most essential pulmonary pathology into a compact, high-yield format! It covers both common and rare neoplastic and non-neoplastic diseases of the lung and pleura and focuses primarily on diagnosis with correlations to clinical and radiographic characteristics. Its pragmatic, well-organized approach, abundant full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice! Detailed discussions on today’s technologies help you select the best test for case evaluation. Chapters devoted to the techniques used in the assessment of pulmonary diseases, including immunohistochemistry, immunofluorescnece, and certain clinical laboratory tests offer you a better understanding of techniques and their application for the diagnosis of lung disease. Internationally recognized pathologists convey the most current information, keeping you on the cusp of your field. More than 800 photomicrographs and gross photographs—most in full color—present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases. Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.