Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine Second Edition written by Leland J. Cseke and published by CRC Press. This book was released on 2003-11-24 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Leland J. Cseke and published by CRC Press. This book was released on 2003-11-24 with total page 598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by and published by . This book was released on 2004 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Donghern Kim and published by CRC Press. This book was released on 1995-05-15 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!
Download or read book Concise Dictionary of Biomedicine and Molecular Biology written by Pei-Show Juo and published by CRC Press. This book was released on 2001-12-21 with total page 1163 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in science, medicine, and molecular biology have created a large amount of new information on biomedicine and molecular biology. Keeping up with the latest information can become a cumbersome task for professionals and students working in these fields. Updated to include new terminology and accurate characterizations of previously ex
Download or read book Handbook of Research on Systems Biology Applications in Medicine written by Daskalaki, Andriani and published by IGI Global. This book was released on 2008-11-30 with total page 982 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book highlights the use of systems approaches including genomic, cellular, proteomic, metabolomic, bioinformatics, molecular, and biochemical, to address fundamental questions in complex diseases like cancer diabetes but also in ageing"--Provided by publisher.
Download or read book Handbook of Systems Biology written by Marian Walhout and published by Academic Press. This book was released on 2012-12-31 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an entry point into Systems Biology for researchers in genetics, molecular biology, cell biology, microbiology and biomedical science to understand the key concepts to expanding their work. Chapters organized around broader themes of Organelles and Organisms, Systems Properties of Biological Processes, Cellular Networks, and Systems Biology and Disease discuss the development of concepts, the current applications, and the future prospects. Emphasis is placed on concepts and insights into the multi-disciplinary nature of the field as well as the importance of systems biology in human biological research. Technology, being an extremely important aspect of scientific progress overall, and in the creation of new fields in particular, is discussed in 'boxes' within each chapter to relate to appropriate topics. - 2013 Honorable Mention for Single Volume Reference in Science from the Association of American Publishers' PROSE Awards - Emphasizes the interdisciplinary nature of systems biology with contributions from leaders in a variety of disciplines - Includes the latest research developments in human and animal models to assist with translational research - Presents biological and computational aspects of the science side-by-side to facilitate collaboration between computational and biological researchers
Download or read book Advanced Methods in Cellular Immunology written by Rafael Fernandez-Botran and published by CRC Press. This book was released on 2000-05-26 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunologists as well investigators in other disciplines may often use protocols involving the isolation, cultures and characterization of different types of leukocytes. Advanced Methods in Cellular Immunology is a collection of techniques in an easy-to-use format. Each chapter provides readers with related program information, a step-by-step de
Download or read book Human Cell Culture Protocols written by Joanna Picot and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calculations for Molecular Biology and Biotechnology written by Frank H. Stephenson and published by Academic Press. This book was released on 2010-07-30 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine Third Edition written by Leland J. Cseke and published by CRC Press. This book was released on 2011-12-12 with total page 739 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.
Download or read book Experimental Design for Biologists written by David J. Glass and published by CSHL Press. This book was released on 2007 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.
Download or read book Handbook of Epigenetics written by Trygve O Tollefsbol and published by Academic Press. This book was released on 2017-07-10 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Epigenetics: The New Molecular and Medical Genetics, Second Edition, provides a comprehensive analysis of epigenetics, from basic biology, to clinical application. Epigenetics is considered by many to be the new genetics in that many biological phenomena are controlled, not through gene mutations, but rather through reversible and heritable epigenetic processes. These epigenetic processes range from DNA methylation to prions. The biological processes impacted by epigenetics are vast and encompass effects in lower organisms and humans that include tissue and organ regeneration, X-chromosome inactivation, stem cell differentiation, genomic imprinting, and aging. The first edition of this important work received excellent reviews; the second edition continues its comprehensive coverage adding more current research and new topics based on customer and reader reviews, including new discoveries, approved therapeutics, and clinical trials. From molecular mechanisms and epigenetic technology, to discoveries in human disease and clinical epigenetics, the nature and applications of the science is presented for those with interests ranging from the fundamental basis of epigenetics, to therapeutic interventions for epigenetic-based disorders. - Timely and comprehensive collection of fully up-to-date reviews on epigenetics that are organized into one volume and written by leading figures in the field - Covers the latest advances in many different areas of epigenetics, ranging from basic aspects, to technologies, to clinical medicine - Written at a verbal and technical level that can be understood by scientists and college students - Updated to include new epigenetic discoveries, newly approved therapeutics, and clinical trials
Download or read book Molecular Biology of the Cell 6E The Problems Book written by John Wilson and published by Garland Science. This book was released on 2014-11-21 with total page 984 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Problems Book helps students appreciate the ways in which experiments and simple calculations can lead to an understanding of how cells work by introducing the experimental foundation of cell and molecular biology. Each chapter reviews key terms, tests for understanding basic concepts, and poses research-based problems. The Problems Book has be
Download or read book Oxford Handbook of Medical Sciences written by Robert Wilkins and published by OUP Oxford. This book was released on 2011-09-22 with total page 990 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by biomedical scientists and clinicians, with the purpose of disseminating the fundamental scientific principles that underpin medicine, this new edition of the Oxford Handbook of Medical Sciences provides a clear, easily digestible account of basic cell physiology and biochemistry. It also includes an investigation of the traditional pillars of medicine (anatomy, physiology, biochemistry, pathology and pharmacology) integrated in the context of each of the major systems relevant to the human body. Cross-referenced to the Oxford Handbook of Clinical Medicine, and thoroughly illustrated, it is the ideal introduction to the medical sciences for medical students and biomedical scientists, as well as a valuable refresher for junior doctors.
