Download or read book The Guide to Investigation of Mouse Pregnancy written by B. Anne Croy and published by Academic Press. This book was released on 2013-12-09 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. - Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications - Describes the angiogenic tree the mother's uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes - Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation - Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success

Download or read book Manipulating the Mouse Embryo written by Brigid Hogan and published by Cold Spring Harbor Laboratory Press. This book was released on 1986 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Download or read book Morphological Mouse Phenotyping written by Jesus Ruberte and published by Elsevier. This book was released on 2017-01-27 with total page 602 pages. Available in PDF, EPUB and Kindle. Book excerpt: Morphological Mouse Phenotyping: Anatomy, Histology and Imaging is an atlas of explanatory diagrams and text that guides the reader through normal mouse anatomy, histology, and imaging. The book is targeted for mouse researchers and veterinarian and human pathologists, and presents a complete, integrative description of normal mouse morphology. Disease animal models are fundamental in research to improve human health. The success of using genetically engineered mice to evaluate molecular disease hypotheses has encouraged the development of massive global projects, making the mouse the most used animal disease model. Laboratory mouse populations are straining the housing capacity of pharmaceutical and biotechnology companies, as well as public research institutions. However, the scientific community lacks sufficient expertise in morphological phenotyping to effectively characterize and validate these animal models. The mouse displays fundamental morphological similarities to humans; however, a mouse is not a man. - Features more than 2,200 original images showing the anatomy, histology, and cellular structure of mouse organs - Includes images specifically produced for this book in the Mouse Imaging Platform (Center for Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona) - Offers an integrative vision of mouse morphology using correlative X-ray, computed tomography, magnetic resonance, and ultrasound images - Employs classical anatomical techniques such as conventional dissection, skeletal preparations, vascular injections, and histological, immunohistochemical, and electron microscopy techniques to characterize mouse morphology

Download or read book Guide for the Care and Use of Laboratory Animals written by National Research Council and published by National Academies Press. This book was released on 2011-01-27 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.

Download or read book Methods of Behavior Analysis in Neuroscience written by Jerry J. Buccafusco and published by CRC Press. This book was released on 2000-08-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using the most well-studied behavioral analyses of animal subjects to promote a better understanding of the effects of disease and the effects of new therapeutic treatments on human cognition, Methods of Behavior Analysis in Neuroscience provides a reference manual for molecular and cellular research scientists in both academia and the pharmaceutic

Download or read book The Laboratory Mouse written by Jennifer Johnson and published by CRC Press. This book was released on 2019-03-18 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Key features: High quality full color photographs and descriptive texts on the location and removal of the organs from the mouse Instructive methods and clear visuals for trimming and orienting the organs for paraffin histology to obtain the best possible sections for analysis Full color photomicrographs of the resulting section for each organ stained with hematoxylin and eosin demonstrating important features and landmarks for the histologist to ensure the optimal area for analysis is achieved All in one, easy to use guide organized by individual organs of the laboratory mouse Spiralbound for easy reference in the lab This "one-stop" guide offers an essential resource for any academic, research or development operation where mouse necropsy and/or histology are performed. Connecting the reader ‘from the mouse to the microscope’, it provides a detailed guide for locating, trimming, orientating and embedding of the most frequently investigated tissues collected in the laboratory mouse. It shows where the organs reside in the mouse, how to trim and embed them as well as the resulting optimal sections. This guide brings together the wealth of scattered information into one high-quality text, the emphasis is on providing knowledge that will help histologists and scientists get better results in any downstream assays where ideal sections are needed.

Download or read book A Practical Guide to the Histology of the Mouse written by Cheryl L. Scudamore and published by John Wiley & Sons. This book was released on 2014-02-10 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues. This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally- induced lesions. This will be an essential bench-side companion for researchers and practitioners looking for an accessible and well-illustrated guide to mouse pathology. Written by experienced pathologists and specifically tailored to the needs of scientists and histologists Full colour throughout Provides advice on sampling tissues, necropsy and recording data Includes common anatomic variations, background lesions and artefacts which will help non-experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation

Download or read book Nutrient Requirements of Laboratory Animals written by National Research Council and published by National Academies Press. This book was released on 1995-02-01 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the years since the third edition of this indispensable reference was published, a great deal has been learned about the nutritional requirements of common laboratory species: rat, mouse, guinea pig, hamster, gerbil, and vole. The Fourth Revised Edition presents the current expert understanding of the lipid, carbohydrate, protein, mineral, vitamin, and other nutritional needs of these animals. The extensive use of tables provides easy access to a wealth of comprehensive data and resource information. The volume also provides an expanded background discussion of general dietary considerations. In addition to a more user-friendly organization, new features in this edition include: A significantly expanded section on dietary requirements for rats, reporting substantial new findings. A new section on nutrients that are not required but that may produce beneficial results. New information on growth and reproductive performance among the most commonly used strains of rats and mice and on several hamster species. An expanded discussion of diet formulation and preparationâ€"including sample diets of both purified and natural ingredients. New information on mineral deficiency and toxicity, including warning signs. This authoritative resource will be important to researchers, laboratory technicians, and manufacturers of laboratory animal feed.

Download or read book Male Germline Stem Cells Developmental and Regenerative Potential written by Kyle E. Orwig and published by Springer Science & Business Media. This book was released on 2010-12-16 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today’s generation of stem cell researchers, including the field’s potential for regenerative medicine. Ranging from an assessment of the pluripotency of primordial germ cells and their possible applications in treating testicular cancer, to the recovery of once-mordant fertilization-competent sperm, this volume has it all. It is a reference point for any scientist involved in related research as well as being a timely summation of what could prove to be a hugely exciting and very fruitful area of inquiry.

Download or read book Methods in Muscle Biology written by Charles Emerson and published by Academic Press. This book was released on 1997 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Muscle Biology is a comprehensive laboratory guide that details the methods used in the study of muscle biology. The techniques included embrace cell, developmental, and molecular biology, as well asphysiology, neurobiology, and medical research.

Download or read book Methods in Methane Metabolism written by Amy Claire Rosenzweig and published by Academic Press. This book was released on 2011 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

Download or read book Methods in Protein Design written by and published by Academic Press. This book was released on 2013-02-14 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers methods in protein design - Contains chapters with such topics as protein switch engineering by domain insertion, evolution-based design of proteins, and computationally designed proteins

Download or read book Somitogenesis Part 1 written by and published by Academic Press. This book was released on 1999-12-06 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chapters contained in this two-volume set provide a broad perspective on the novel strategies and conceptual paradigms that drive the current resurgence of interest in somitogenesis - the process by which somites form and elaborate differentiated tissues and structures. Because somites are a ubiquitous feature of vertebrate embryos, they can be studied in a variety of experimental animal models including those amenable to genetic (zebrafish, mammalian), molecular/genetic (mammalian, avian) as well as those already well established for classical experimental embryological and cell biological studies (amphibians, avian). The wide variety of experimental approaches to somitogenesis that are presented in these volumes will leave the reader with a broad perspective on how current research in somitogenesis is helping to solve fundamental questions in vertebrate development and morphogenesis.Key Features:* Novel transcriptional mechanisms that control repetitive pattern formation* Wide scale genetic screens for mutations affecting somitogenesis* Molecular/genetic control of pattern and tissue formation during somitogenesis* Transplantation of mouse embryo somites* Classical embryological approaches and concepts* Evolutionary perspectives on somitogenesis

Download or read book Molecular and Cellular Methods in Developmental Toxicology written by George P. Daston and published by CRC Press. This book was released on 2019-04-30 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

Download or read book Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research written by National Research Council and published by National Academies Press. This book was released on 2003-08-22 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.

Download or read book Methods in Systems Biology written by and published by Academic Press. This book was released on 2011-09-19 with total page 775 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research. - This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology - With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research