Download or read book Hormonally Defined Media written by G. Fischer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until some years ago serum or crude tissue extracts were used pre dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re gard to survival, proliferation and differentiation of cells in cul ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.

Download or read book Growth of Cells in Hormonally Defined Media written by Gordon Sato and published by . This book was released on 1982 with total page 1214 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by David A. Sirbasku and published by . This book was released on 1981 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by Gordon Sato and published by . This book was released on 1982 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Hormonally Defined Media written by Günther Fischer and published by Springer Verlag. This book was released on 1983 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by Gordon Sato and published by . This book was released on 1982 with total page 790 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by David A. Sirbasku and published by . This book was released on 1981 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media Ed by Gordon H Sato Arthur B Pardee and David A Sirbasku written by Gordon H. Sato and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mammalian Cell Culture written by Jennie Mather and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advantages of obtaining a completely defined environment for the growth of cells in vitro were recognized very early in the history of cell culture (Lewis and Lewis, 1911). Continued interest in the nutritional requirements of cells in vitro and in providing an optimal environment for cells led to the development of the complex nutrient mixtures available today in many media (Waymouth, 1972; Ham, 1965). However, serum remained an essential component of medium for the growth of most cell types in culture. The question of what factor (or factors) in serum was essential for cell growth and survival remained unanswered for several decades. Initially, experiments were designed to purify the "active component" of serum for the growth of cells in culture. These experiments identified fetuin (Fisher et at., 1958) and nonsuppressible insulinlike activity (Temin et at., 1972) as important components of serum. However, the complexity of serum and the very low levels of active components in serum hindered progress in identi fying and isolating serum factors.

Download or read book Abstracts of Papers Presented at the Ninth Cold Spring Harbor Conference on Cell Proliferation written by and published by . This book was released on 1981 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by David Andrew Sirbasku and published by . This book was released on 1982 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Effects of Hormonally Defined Media on the Cellular Growth and Gene Expression of Transformed Liver Cells written by Iliana Fred-García and published by . This book was released on 1989 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth of Cells in Hormonally Defined Media written by Gordon Sato and published by . This book was released on 1982 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Hormonally Defined Media written by Günther Fischer and published by . This book was released on 1983 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: