Download or read book Methods on Glycoconjugates written by André Verbert and published by Harwood Academic Publishers. This book was released on 1995-07-01 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods On Glycoconjugates written by André Verbert and published by CRC Press. This book was released on 1995-07-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: By guiding the reader toward answering basic questions on the structural analysis of glycoconjugates using conventional techniques, this book studies methods such as chemical cleavage, molar composition, use of enzymes, use of lectins and typical fractionation procedures such as HPLC, GLC, TLC, and other chromatographic systems. More sophisticated techniques such as NMR or MS are described to give the reader the essential information needed to adapt procedures to his/her requirements. This book will serve as a practical manual designed for scientists not familiar with glycoconjugate methodologies, but whose research work requires contact with analytical problems or metabolic investigations in glycobiology. It is the result of twenty years of experience of summer schools held in a laboratory environment and will acquaint the reader with widely used techniques in the area rather than cataloguing an exhaustive list of methods. This will help any biochemist to develop an experiment with ease.
Download or read book Coupling and Decoupling of Diverse Molecular Units in Glycosciences written by Zbigniew J. Witczak and published by Springer. This book was released on 2018-08-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book covers the latest developments in coupling and decoupling of biomolecules containing functionalized carbohydrate components, being one of the first collections in this important area of applied medicinal chemistry. Connecting molecules, often referred as bio-conjugation, has become one of the most often performed procedures in modern medicinal chemistry. Sometimes, when the connected molecules are not useful anymore, they must be disconnected. The molecules that must be connected (coupled) may belong to both small and large molecules and include such constructs as glycoproteins, glycopeptides and glycans. In this work, more than 15 experts address a comprehensive range of potential and current uses of in vitro and in vivo bio-conjugation methodologies, leading to a variety of glycoconjugates. The analytical aspects of bio-conjugation are also here discussed. Medicinal and organic chemists from graduate level onwards will understand the appeal of this important book.
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Download or read book Nuclear Magnetic Resonance of Biological Macromolecules Part C written by and published by Academic Press. This book was released on 2005-04-20 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Download or read book Modern Synthetic Methods in Carbohydrate Chemistry written by Daniel B. Werz and published by John Wiley & Sons. This book was released on 2013-12-23 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fields of glycochemistry and glycoscience are rich and varied and where much can be learned from Nature. As Nature is not always able to produce carbohydrates in quantities useful for not only in research but also as therapeutic agents, new ways need to be found to optimize the yield. This book presents an overview of the latest developments in the field of carbohydrates, ranging from de-novo approaches via cyclodextrin chemistry to the synthesis of such highly complex glycoconjugates as glycosphingolipids and GPI anchors. The main emphasis remains on the synthetic aspects making the book an excellent source of information for those already involved in carbohydrate chemistry, as well as for those organic chemists who are beginners in this field. Equally of interest to synthetic chemists, as well as medicinal chemists and biochemists.
Download or read book Glycoconjugates written by Howard J. Allen and published by CRC Press. This book was released on 1992-04-15 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycoconjugates Composition: Structure, and Function provides an excellent overview of the composition, biosynthesis, function and structure of the carbohydrate chains of glycoconjugates from higher organisms. It is recommended as a core reference text, providing excellent coverage of the glycoconjugate field.
Download or read book The Glycoconjugates written by Martin Horowitz and published by Elsevier. This book was released on 2012-12-02 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Glycoconjugates: Mammalian Glycoproteins and Glycolipids, Volume I is a collaboration of different experts in the field of molecular biology on the subject of glycoconjugates. The text discusses the historical aspect, nomenclature, biosynthesis, and catabolism of glycoconjugates and their role in normal and pathological processes. Also covered are topics such as the purification of glycoproteins; the elucidation of the amino acid sequence of glycoproteins; the glycoproteins found in the different parts of the human body; glycoenzymes; and glycohormones. The book is recommended for molecular biologists, organic chemists, and biochemists who would like to know more about glycolipids and glycoproteins and their applications.
Download or read book Glycoconjugate Research written by John Gregory and published by Elsevier. This book was released on 2012-12-02 with total page 622 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycoconjugate Research, Volume I contains the proceedings of the Fourth International Symposium on Glycoconjugates, held in Woods Hole, Massachusetts, in September 1977. Contributors focus on the state of knowledge in the field of glycoconjugates ranging from polysaccharides and glycoproteins to glycolipids, proteoglycans, and all their varieties in plants, animals, and microorganisms. This text is organized into three sections and consists of 87 chapters. After an introductory chapter where the use of glycosidases for the structural analysis of complex carbohydrates is described, the discussion turns to other methods used for elucidating the structure of complex carbohydrates. The chapters that follow focus on the primary structure and conformation of glycans that are N-glycosically linked to peptide chains; isolation of monofuco-disialoganglioside from human and bovine brains; and partial chemical characterization of activated peptides isolated from a single cell suspension of rat colonic secretory cells. The last two sections explore cartilage proteoglycans and the structure-function relationships of glycoconjugates, touching on topics such as link proteins; the biochemical aspects of malignancy in human chondrosarcomas; defensive response of plants to a microbial oligosaccharide with a pheromone-like activity; and the interaction of saccharides with ricin. This book is intended for students and practitioners of chemistry and biochemistry; biology and microbiology; and physiology.
Download or read book Carbohydrates written by Helen Osborn and published by Academic Press. This book was released on 2003-04-22 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a vast and often bewildering array of synthetic methods and reagents available to organic chemists today. The Best Synthetic Methods series allows the practising synthetic chemist to choose between all the alternatives and assess their real advantages and limitations. Each chapter in Carbohydrates details a particular theme associated with carbohydrate synthesis. A brief review of the subject area is provided, but the emphasis in all cases is on describing efficient practical methods to effect the transformations described. In order for the roles of carbohydrates to be thoroughly analysed and assessed, glycobiologists require access to defined target carbohydrates in useful quantities. Thus carbohydrates and glycoconjugates are now recognized as important targets for total synthesis programmes and it is essential to develop efficient regio- and stereoselective methods for the synthesis of carbohydrates. Whilst carbohydrates can sometimes be isolated from natural sources, synthetic strategies often offer the advantage of allowing access to larger quantities of material as well as entry to analogues of the natural carbohydrates. The latest volume in the long standing Best Synthetic Methods series Clear chapter by chapter breakdown of carbohydrate synthesis themes with examples of good practical methods for common carbohydrate syntheses
Download or read book Glycoconjugates and Methods written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.
Download or read book Modern Methods in Carbohydrate Synthesis written by Shaheer H. Khan and published by CRC Press. This book was released on 2020-08-26 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Methods in Carbohydrate Synthesis presents in one volume a sequence of chapters leading from classical methods through to today's newest state-of -the-art technology for oligosaccharide synthesis. It places particular emphasis on the most recent breakthroughs in the field, including emerging technologies for both oligosaccharide and glycoconjugate synthesis. Chapters describing the synthesis of increasingly important glycosidic linkage analogs, as well as the oligosaccharides containing derivatives and analogs of natural sugars are included. While chemical-synthetic methods constitute the major part of the book, completing the volume is a section on the rapidly expanding and important field of enzymatic synthesis, also covering combined chemical and enzymatic synthesis. Chapters are written by leading experts in the field. Wherever possible, methods of synthesis are provided in sufficient detail to allow the reader to implement the techniques described. More than 1700 references are provided in the 21 chapters comprising the book. This volume should provide a wealth of information to a large number of synthetic organic chemists, medicinal chemists, protein chemists, biochemists, glycobiologists and cell biologists, including students in these fields.
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Download or read book Synthesis and Characterization of Glycosides written by Marco Brito-Arias and published by Springer Nature. This book was released on 2022-04-22 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition is a comprehensive and extended study about the best known approaches for preparing the main types of glycosides, covering the classic and more recent glycosylation reactions used for preparing simple and challenging glycosides currently used as potent antiviral and antineoplastic drugs, or fluorogenic substrates used for enzymatic detection in cell biology. Besides, this new edition provides more examples of the glycosidic methodologies followed for preparing complex glycoconjugates such as glycoproteins and glycosphingolipids and gangliosides used as adjuvants or as synthetic vaccines candidates. Also, additional mechanistic evidence is presented for better understanding of the glycosylation reaction, trying to identify the variables mainly depending on protecting and leaving groups, as well as catalyst and reaction condition which altogether directs the anomeric stereo control. A chapter on the glycoside hydrolysis is included in view of the increasing interest in the use of biomass as a natural and renewable source for obtaining important intermediates or products used in food or valuable materials. The author includes information in the characterization of glycosides section with the aim of giving additional tools for the structural assignment through NMR, X-Ray and mass spectra techniques.
Download or read book Mass Spectrometry in Polymer Chemistry written by Christopher Barner-Kowollik and published by John Wiley & Sons. This book was released on 2012-01-27 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combining an up-to-date insight into mass-spectrometric polymer analysis beyond MALDI with application details of the instrumentation, this is a balanced and thorough presentation of the most important and widely used mass-spectrometric methods. Written by the world's most proficient experts in the field, the book focuses on the latest developments, covering such technologies and applications as ionization protocols, tandem and liquid chromatography mass spectrometry, gas-phase ion-separation techniques and automated data processing. Chapters on sample preparation, polymer degradation and the usage of mass-spectrometric tools on an industrial scale round off the book. As a result, both entrants to the field and experienced researchers are able to choose the appropriate methods and instrumentations -- and to assess their respective strengths and limitations -- for the characterization of polymer compounds.
Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. Covers the basic principles of modern liquid phase separation techniques, along with their applications Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Download or read book Site specific Methods for Preparation of Glycoconjugates written by Jonathan Peter Dolan and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
