Download or read book Genotype Phenotype Coupling written by Stefan Zielonka and published by Springer Nature. This book was released on 2023-07-05 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.
Download or read book Genotype Phenotype Coupling written by Stefan Zielonka and published by . This book was released on 2020 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field.
Download or read book A Probabilistic Model of the Genotype Phenotype Relationship written by Jean-Pierre Hugot and published by CRC Press. This book was released on 2018-06-19 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Probabilistic Model of the Genotype/Phenotype Relationship provides a new hypothesis on the relationship between genotype and phenotype. The main idea of the book is that this relationship is probabilistic, in other words, the genotype does not fully explain the phenotype. This idea is developed and discussed using the current knowledge on complex genetic diseases, phenotypic plasticity, canalization and others.
Download or read book Evolutionary Calculus of the Genotype phenotype Relationship written by Teng-Keui Hsu and published by . This book was released on 2019 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Bioinformatics written by Cathy H. Wu and published by Humana. This book was released on 2017-02-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces bioinformatics research methods for proteins, with special focus on protein post-translational modifications (PTMs) and networks. This book is organized into four parts and covers the basic framework and major resources for analysis of protein sequence, structure, and function; approaches and resources for analysis of protein PTMs, protein-protein interactions (PPIs) and protein networks, including tools for PPI prediction and approaches for the construction of PPI and PTM networks; and bioinformatics approaches in proteomics, including computational methods for mass spectrometry-based proteomics and integrative analysis for alternative splice isoforms, for functional discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory or computational protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Bioinformatics: From Protein Modifications and Networks to Proteomics is a valuable resource for readers who wish to learn about state-of-the-art bioinformatics databases and tools, novel computational methods, and future trends in protein and proteomic data analysis in systems biology. This book is useful to researchers who work in the biotechnology and pharmaceutical industries, and in various academic departments, such as biological and medical sciences and computer sciences and engineering.
Download or read book Cell Language Theory The Connecting Mind And Matter written by Sungchul Ji and published by World Scientific. This book was released on 2017-11-24 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book represents the results of 45 years of research on a wide range of topics, including atomic physics, single-molecule enzymology, whole-cell metabolism, physiology, pharmacology, linguistics, semiotics, and cosmology. It describes the first comprehensive molecular theory of the genotype-phenotype coupling based on two key theoretical concepts: (i) the conformon, the conformational wave packet in biopolymers carrying both the free energy and genetic information; and (ii) the intracellular dissipative structures, the chemical concentration waves inside the cell that serve as the immediate drivers of all cell functions. Conformons provide the driving forces for all molecular machines in the cell, and intracellular dissipative structures coordinate intra- and intercellular processes such as gene expression and cell-cell communications.One of the predictions made by the cell language theory (CLT) is that there are two forms of genetic information — the Watson-Crick genes transmitting information in time (identified with DNA), and the Prigoginian genes transmitting information in space (identified with RNA expression profiles). The former is analogous to sheet music or written language and the latter is akin to audio music or spoken language, both being coupled by conformons acting as the analog of the pianist. The new theory of DNA structure and function constructed on the basis of CLT can rationally account for most of the puzzling findings recently unearthed by the ENCODE (Encyclopedia of DNA Elements) project.The Cell Language Theory has important applications in biomedical sciences including drug discovery research and personalized medicine on the one hand and in the mind-body research and consciousness studies on the other.
Download or read book Immunophenotyping written by J Philip McCoy Jr and published by Humana. This book was released on 2019-09-29 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.
Download or read book Cell Cell Interactions written by Sean P. Colgan and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics written by Yi Pan and published by John Wiley & Sons. This book was released on 2013-11-12 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics An in-depth look at the latest research, methods, and applications in the field of protein bioinformatics This book presents the latest developments in protein bioinformatics, introducing for the first time cutting-edge research results alongside novel algorithmic and AI methods for the analysis of protein data. In one complete, self-contained volume, Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics addresses key challenges facing both computer scientists and biologists, arming readers with tools and techniques for analyzing and interpreting protein data and solving a variety of biological problems. Featuring a collection of authoritative articles by leaders in the field, this work focuses on the analysis of protein sequences, structures, and interaction networks using both traditional algorithms and AI methods. It also examines, in great detail, data preparation, simulation, experiments, evaluation methods, and applications. Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics: Highlights protein analysis applications such as protein-related drug activity comparison Incorporates salient case studies illustrating how to apply the methods outlined in the book Tackles the complex relationship between proteins from a systems biology point of view Relates the topic to other emerging technologies such as data mining and visualization Includes many tables and illustrations demonstrating concepts and performance figures Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics is an essential reference for bioinformatics specialists in research and industry, and for anyone wishing to better understand the rich field of protein bioinformatics.
Download or read book Bioinformatics and Drug Discovery written by Richard S. Larson and published by . This book was released on 2012 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in drug discovery have been rapid. The second edition of Bioinformatics and Drug Discovery has been completely updated to include topics that range from new technologies in target identification, genomic analysis, cheminformatics, protein analysis, and network or pathway analysis. Each chapter provides an extended introduction that describes the theory and application of the technology. In the second part of each chapter, detailed procedures related to the use of these technologies and software have been incorporated. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Bioinformatics and Drug Discovery, Second Edition seeks to aid scientists in the further study of the rapidly expanding field of drug discovery.
Download or read book Serial Analysis of Gene Expression SAGE written by Kåre Lehmann Nielsen and published by Springer Science & Business Media. This book was released on 2008 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.
Download or read book Genetics written by Daniel L. Hartl and published by Jones & Bartlett Publishers. This book was released on 2012 with total page 830 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Population Genetics and Genomics written by Alan R. Templeton and published by Academic Press. This book was released on 2018-11-08 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Population Genetics and Genomics provides researchers/students with knowledge on population genetics and relevant statistical approaches to help them become more effective users of modern genetic, genomic and statistical tools. In-depth chapters offer thorough discussions of systems of mating, genetic drift, gene flow and subdivided populations, human population history, genotype and phenotype, detecting selection, units and targets of natural selection, adaptation to temporally and spatially variable environments, selection in age-structured populations, and genomics and society. As human genetics and genomics research often employs tools and approaches derived from population genetics, this book helps users understand the basic principles of these tools. In addition, studies often employ statistical approaches and analysis, so an understanding of basic statistical theory is also needed. - Comprehensively explains the use of population genetics and genomics in medical applications and research - Discusses the relevance of population genetics and genomics to major social issues, including race and the dangers of modern eugenics proposals - Provides an overview of how population genetics and genomics helps us understand where we came from as a species and how we evolved into who we are now
Download or read book Computational Biology written by Ralf Blossey and published by CRC Press. This book was released on 2006-05-25 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative methods have a particular knack for improving any field they touch. For biology, computational techniques have led to enormous strides in our understanding of biological systems, but there is still vast territory to cover. Statistical physics especially holds great potential for elucidating the structural-functional relationships in bi
Download or read book Systemic Approaches in Bioinformatics and Computational Systems Biology written by Paola Lecca and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book presents new techniques that have resulted from the application of computer science methods to the organization and interpretation of biological data, covering three subject areas: bioinformatics, computational biology, and computational systems biology"--
Download or read book Molecular Methods for Evolutionary Genetics written by Virginie Orgogozo and published by Humana Press. This book was released on 2011-03-15 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.
Download or read book Cricket Behavior and Neurobiology written by Franz Huber and published by Cornell University Press. This book was released on 2019-05-15 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: The world of crickets has long been a world of scientific adventure and human fascination. Because of their remarkable ways of communicating and because their nervous and endocrine systems are easily accessible to researchers, crickets can be studied and analyzed with great effectiveness. Starting in the 1960s, vastly improved behavioral and neurobiological techniques have brought them to the frontier of the new field of neuroethology. Here, in the most comprehensive book on crickets ever compiled, twenty-five leading scientists detail the present state of cricket research both at conceptual and at experimental levels. They tell about the manifold strategies crickets use in matching development with seasons and habitats, finding mates, and avoiding parasites and predators, and they describe the physiological mechanisms, especially the neuronal mechanisms, underlying cricket behavior. Their book is at once about communication, comparative physiology and anatomy, and environmental interaction. More than half of Cricket Behavior and Neurobiology is devoted to acoustic behavior and bioacoustics. It is intended for those interested in entomology, general and comparative physiology, biophysics, endocrinology, and chronobiology. It offers new information for behavioral physiologists and ecologists, bioacousticians, and especially neurobiologists concerned with behavior.
