Download or read book In Vitro Selection and Genetic Transformation of Wheat Triticum aestivum L written by ZAKI AHMED. KASEM and published by . This book was released on 1992 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Haploid Production and Genetic Transformation of Wheat Triticum Aestivum L written by Heidi M. Triggs and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Transformation of Wheat Triticum Aestivum L written by Zainu'ddin and published by . This book was released on 2000 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The successful application of genetic engineering in wheat is dependent on the availability of suitable tissue culture and transformation methods. The primary object of this project was the development of these technologies using elite Australian wheat varieties.

Download or read book Genetic Transformation of Wheat Triticum Aestivum L for Abiotic Stress Tolerance written by K. Lakshmi and published by . This book was released on 2008 with total page 73 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Transformation Studies in Bread Wheat Triticum Aestivum L written by A. Ramesha and published by . This book was released on 2003 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Single stranded DNA as a Tool for Genetic Transformation in Wheat Triticum Aestivum L written by Murielle Uzé and published by . This book was released on 1997 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Wheat Genetics From Genome to Field written by Yasunari Ogihara and published by Springer. This book was released on 2015-09-15 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.

Download or read book Biolistic DNA Delivery in Plants written by Sachin Rustgi and published by Humana. This book was released on 2021-04-25 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.

Download or read book Microspore Embryogenesis in Wheat Triticum Aestivum L and Genetic Transformation of Wheat and Barley Hordeum Vulgare L Microspores Using the Gfp Marker Gene written by Hans Hönicka and published by . This book was released on 2002 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification of Target Cells in Immature Embryos of Wheat Triticum Aestivum L for Genetic Transformation and Optimization of DNA Delivery by Biolistics Devices written by Dong Yul Sung and published by . This book was released on 1996 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Transformation of Bread Wheat Triticum Aestivium L with a Murine gamma herpesvirus Glycoprotein B Gene written by Tiruneh Kefyalew Hailemariam and published by . This book was released on 2002 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Anthers of the bread wheat (Triticum aestivium L.) varieties Pavon and Bobwhite were isolated and cultured in liquid CHB-2 medium. The resulting embryoids were used for transient and/or stable expression studies using the pAHC27 and pAHC25 plasmids through microprojectile bombardment. Regeneration studies were also conducted with hormone supplements using Bobwhite embryoids. Results show that Pavon anthers have better embryogenic induction capacity than Bobwhite anthers. Supplementing 3-Indolyl Acetic Acid in the regeneration medium improved the regeneration capacity and reduced the frequency of albino regenerants of Bobwhite as compared to the use of medium alone. Moreover, we modified the pAHC25 plasmid so that it carries the murine gammaherpesvirus-68 ([gamma]HV-68) glycolprotein B (gB) gene along with the selectable bar gene and used it for stable transformation study with the intention to express the immunogen in wheat as a potential edible vaccine. PCR analysis indicated the presence of the gene in bombarded embryoids after 2 weeks. But, no RT-PCR or Western blot products were detected from extracts of these embryoids. The pTH2 transformed embryoids were regenerated on selection medium and some putative transformants have been recovered. Tissues of these potentially transgenic plants can be orally administered to mice to test if they induce immunologic response against gammaherpes virus.

Download or read book Alien Gene Transfer in Crop Plants Volume 1 written by Aditya Pratap and published by Springer Science & Business Media. This book was released on 2013-11-01 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The 1st volume entitled, ‘Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment” will deal exclusively with the process and methodology. The contents of this volume have been designed to appraise the readers with all the theoretical and practical aspects of wide hybridization and gene transfer like processes and methods of gene transfer, role of biotechnology with special reference to embryo rescue, genetic transformation, protoplast fusion and molecular marker technology, problems such as cross incompatibility and barriers to distant hybridization and solutions to overcome them. Since wild and weedy relatives of crop plants may have negative traits associated with them, there are always possibilities of linkage drag while transferring alien alleles. Therefore, problems and limitations of alien gene transfer from these species will also be discussed in this series. Further, the associated risks with this and assessment of risks will also be given due weightage.

Download or read book Wheat Transformation Technologies written by Simmonds, John Alfred and published by . This book was released on 1993 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics and Genomics of the Triticeae written by Catherine Feuillet and published by Springer Science & Business Media. This book was released on 2009-06-10 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequencing of the model plant genomes such as those of A. thaliana and rice has revolutionized our understanding of plant biology but it has yet to translate into the improvement of major crop species such as maize, wheat, or barley. Moreover, the comparative genomic studies in cereals that have been performed in the past decade have revealed the limits of conservation between rice and the other cereal genomes. This has necessitated the development of genomic resources and programs for maize, sorghum, wheat, and barley to serve as the foundation for future genome sequencing and the acceleration of genomic based improvement of these critically important crops. Cereals constitute over 50% of total crop production worldwide (http://www.fao.org/) and cereal seeds are one of the most important renewable resources for food, feed, and industrial raw materials. Crop species of the Triticeae tribe that comprise wheat, barley, and rye are essential components of human and domestic animal nutrition. With 17% of all crop area, wheat is the staple food for 40% of the world’s population, while barley ranks fifth in the world production. Their domestication in the Fertile Crescent 10,000 years ago ushered in the beginning of agriculture and signified an important breakthrough in the advancement of civilization. Rye is second after wheat among grains most commonly used in the production of bread and is also very important for mixed animal feeds. It can be cultivated in poor soils and climates that are generally not suitable for other cereals. Extensive genetics and cytogenetics studies performed in the Triticeae species over the last 50 years have led to the characterization of their chromosomal composition and origins and have supported intensive work to create new genetic resources. Cytogenetic studies in wheat have allowed the identification and characterization of the different homoeologous genomes and have demonstrated the utility of studying wheat genome evolution as a model for the analysis of polyploidization, a major force in the evolution of the eukaryotic genomes. Barley with its diploid genome shows high collinearity with the other Triticeae genomes and therefore serves as a good template for supporting genomic analyses in the wheat and rye genomes. The knowledge gained from genetic studies in the Triticeae has also been used to produce Triticale, the first human made hybrid crop that results from a cross between wheat and rye and combines the nutrition quality and productivity of wheat with the ruggedness of rye. Despite the economic importance of the Triticeae species and the need for accelerated crop improvement based on genomics studies, the size (1.7 Gb for the bread wheat genome, i.e., 5x the human genome and 40 times the rice genome), high repeat content (>80%), and complexity (polyploidy in wheat) of their genomes often have been considered too challenging for efficient molecular analysis and genetic improvement in these species. Consequently, Triticeae genomics has lagged behind the genomic advances of other cereal crops for many years. Recently, however, the situation has changed dramatically and robust genomic programs can be established in the Triticeae as a result of the convergence of several technology developments that have led to new, more efficient scientific capabilities and resources such as whole-genome and chromosome-specific BAC libraries, extensive EST collections, transformation systems, wild germplasm and mutant collections, as well as DNA chips. Currently, the Triticeae genomics "toolbox" is comprised of: - 9 publicly available BAC libraries from diploid (5), tetraploid (1) and hexaploid (3) wheat; 3 publicly available BAC libraries from barley and one BAC library from rye; - 3 wheat chromosome specific BAC libraries; - DNA chips including commercially available first generation chips from AFFYMETRIX containing 55’000 wheat and 22,000 barley genes; - A large number of wheat and barley genetic maps that are saturated by a significant number of markers; - The largest plant EST collection with 870’000 wheat ESTs, 440’000 barley ESTs and about 10’000 rye ESTs; - Established protocols for stable transformation by biolistic and agrobacterium as well as a transient expression system using VIGS in wheat and barley; and - Large collections of well characterized cultivated and wild genetic resources. International consortia, such as the International Triticeae Mapping Initiative (ITMI), have advanced synergies in the Triticeae genetics community in the development of additional mapping populations and markers that have led to a dramatic improvement in the resolution of the genetic maps and the amount of molecular markers in the three species resulting in the accelerated utilization of molecular markers in selection programs. Together, with the development of the genomic resources, the isolation of the first genes of agronomic interest by map-based cloning has been enabled and has proven the feasibility of forging the link between genotype and phenotype in the Triticeae species. Moreover, the first analyses of BAC sequences from wheat and barley have allowed preliminary characterizations of their genome organization and composition as well as the first inter- and intra-specific comparative genomic studies. These later have revealed important evolutionary mechanisms (e.g. unequal crossing over, illegitimate recombination) that have shaped the wheat and barley genomes during their evolution. These breakthroughs have demonstrated the feasibility of developing efficient genomic studies in the Triticeae and have led to the recent establishment of the International Wheat Genome Sequencing Consortium (IWGSC) (http//:www.wheatgenome.org) and the International Barley Sequencing Consortium (www.isbc.org) that aim to sequence, respectively, the hexaploid wheat and barley genomes to accelerate gene discovery and crop improvement in the next decade. Large projects aiming at the establishment of the physical maps as well as a better characterization of their composition and organization through large scale random sequencing projects have been initiated already. Concurrently, a number of projects have been launched to develop high throughput functional genomics in wheat and barley. Transcriptomics, proteomics, and metabolomics analyses of traits of agronomic importance, such as quality, disease resistance, drought, and salt tolerance, are underway in both species. Combined with the development of physical maps, efficient gene isolation will be enabled and improved sequencing technologies and reduced sequencing costs will permit ultimately genome sequencing and access to the entire wheat and barley gene regulatory elements repertoire. Because rye is closely related to wheat and barley in Triticeae evolution, the latest developments in wheat and barley genomics will be of great use for developing rye genomics and for providing tools for rye improvement. Finally, a new model for temperate grasses has emerged in the past year with the development of the genetics and genomics (including a 8x whole genome shotgun sequencing project) of Brachypodium, a member of the Poeae family that is more closely related to the Triticeae than rice and can provide valuable information for supporting Triticeae genomics in the near future. These recent breakthroughs have yet to be reviewed in a single source of literature and current handbooks on wheat, barley, or rye are dedicated mainly to progress in genetics. In "Genetics and Genomics of the Triticeae", we will aim to comprehensively review the recent progress in the development of structural and functional genomics tools in the Triticeae species and review the understanding of wheat, barley, and rye biology that has resulted from these new resources as well as to illuminate how this new found knowledge can be applied for the improvement of these essential species. The book will be the seventh volume in the ambitious series of books, Plant Genetics and Genomics (Richard A. Jorgensen, series editor) that will attempt to bring the field up-to-date on the genetics and genomics of important crop plants and genetic models. It is our hope that the publication will be a useful and timely tool for researchers and students alike working with the Triticeae.

Download or read book Handbook of Soil Acidity written by Zdenko Rengel and published by CRC Press. This book was released on 2003-01-17 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses the control, management and reduction of soil acidification in various agricultural systems. The text presents strategies to modify and adjust crop production processes to decrease the toxicity of soil contaminants, balance soil pH, improve nutrient uptake and increase yield.

Download or read book Doubled Haploid Production in Crop Plants written by M. Maluszynski and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.

Download or read book Genetic Modification of Plants written by Frank Kempken and published by Springer Science & Business Media. This book was released on 2009-12-15 with total page 683 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book’s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.