Download or read book Genetic Engineering with PCR written by Robert M. Horton and published by Horizon Press. This book was released on 1998-01-01 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Introduction to Genetic Engineering written by Desmond S. T. Nicholl and published by Cambridge University Press. This book was released on 2002-02-07 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
Download or read book PCR Cloning Protocols written by Bruce Alan White and published by . This book was released on 1997 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.
Download or read book PCR Technology written by Henry Erlich and published by Springer. This book was released on 2015-12-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Download or read book Genetic Engineering written by Anil Kumar and published by Nova Publishers. This book was released on 2005 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has a distinguishing feature of having condensed material with adequate information on genetic engineering especially of the microbes. The book covers almost all the topics of genetic engineering for the graduate, postgraduate students and young research scholars of biological sciences. The book is written as per syllabus of genetic engineering paper for Masters course in biotechnology, biochemistry, life sciences of most of the universities. The book is much useful for the students of Masters degree. Emphasis is given on the basic fundamentals. The book contains twelve chapters starting from ' Isolation, purification and estimation of nucleic acids' as chapter 1. The chapter describes general techniques for the isolation and purification of DNA as well as RNA. It also describes methods for quantitative estimation of the nucleic acids. The second chapter describes general characteristics of the vectors used in genetic engineering and also the general account of commonly used individual vectors. The chapter also describes expression vectors. The third chapter describes various commonly used restriction endonucleases. The fourth chapter describes commonly used enzymes in genetic engineering viz. Reverse transcriptase, DNA polymerase I, polynucleotide kinase, teminal dcoxynucleotidyl transferase, alkaline phosphatase, SI nuclease, DNA ligase etc. The fifth chapter describes electrophoresis for the separation of nucleic acids fragments. The sixth chapter is of cloning strategies. It describes construction of genomic DNA library , chromosomal walking, cDNA library, cDNA cloning. The seventh chapter describes DNA sequencing techniques and includes chemical modification method of Maxam and Gilbert, dideoxy sequencing method of Sanger, modifications of chain terminator sequencing, analysis of the sequencing data. The eighth chapter includes various methods of site directed mutagenesis. The ninth chapter describes polymerase chain reaction (PCR). It also includes primer designing and various types of polymerase chain reactions viz. reverse transcriptase polymerase chain reaction (RT-PCR), nested PCR, multiplex PCR etc. Besides, there are chapters 10, 11 and 12 on gene therapy, human genome and proteomics. At the end, glossary has been put which explains main terms used in genetic engineering. One of the important factor introduced in the book is the chapter structure given in the beginning of each chapter that provides, at a glance, the contents of the whole chapter which offers a better learning mechanism. Each chapter is also presented with an introduction that covers the concept of the whole chapter in brief and offers clear understanding of the subject matter to the students. The author on the basis of his experience in teaching genetic engineering at the university level for more than a decade has offered the text in an easily understandable form to the postgraduate students. The book should be of invaluable help to the students, researchers and all those interested in understanding genetic engineering.
Download or read book PCR Cloning Protocols written by Bruce A. White and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.
Download or read book Gene Cloning and Analysis written by B.C. Schaefer and published by Taylor & Francis. This book was released on 2023-04-28 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic
Download or read book PCR Cloning Protocols written by Bing-Yuan Chen and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Download or read book PCR Applications written by Michael A. Innis and published by Academic Press. This book was released on 1999-05-11 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Key Features * Focuses on gene discovery, genomics, and DNA array technology * Covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters * Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval
Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Download or read book Polymerase Chain Reaction written by Patricia Hernandez-Rodriguez and published by BoD – Books on Demand. This book was released on 2012-05-30 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.
Download or read book DNA polymerases in Biotechnology written by Zvi Kelman and published by Frontiers Media SA. This book was released on 2015-03-18 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Download or read book Biotechnology Proteins to PCR written by David W. Burden and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book PCR Technology written by Henry Erlich and published by Palgrave Macmillan. This book was released on 1989-10-01 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Download or read book Genetics and Genetic Engineering written by Tomas Cannon and published by Scientific e-Resources. This book was released on 2018-12-01 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and Genetic Engineering explores the great discoveries in genetics-the study of genes and the inherited information they contain. Genetic engineering alters the genetic make-up of an organism using techniques that remove heritable material or that introduce DNA prepared outside the organism either directly into the host or into a cell that is then fused or hybridized with the host. This involves using recombinant nucleic acid (DNA or RNA) techniques to form new combinations of heritable genetic material followed by the incorporation of that material either indirectly through a vector system or directly through micro-injection, macro-injection and micro-encapsulation techniques. Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genes using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating or copying the genetic material of interest using recombinant DNA methods or by artificially synthesizing the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinent DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40with the lambda virus. As well as inserting genes, the process can be used to remove, or "e;knock out"e;, genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome. This book will prove equally useful for physicians, nurses, animal breeders, and laboratory technicians-in fact, everyone whose daily work involves genetics and genetic engineering.
Download or read book Recombinant DNA Technology written by Keya Chaudhuri and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.
Download or read book Application of PCR Technology to Plant Breeding written by Andrew Kalinski and published by DIANE Publishing. This book was released on 1995-07 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt:
