Download or read book Genetic and Biochemical Studies of Mitochondria in the Yeast Saccharomyces Cerevisiae written by K. Allen G. Donald and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Studies of the Group II Intron AI2 RNP Particle in the Mitochondria of the Bakers Yeast Saccharomyces Cerevisiae written by Michael Yi Chao and published by . This book was released on 2001 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Genetic Studies of Mitochondrial Protein Synthesis in Saccharomyces Cerevisiae written by Changkeun Lee and published by . This book was released on 2008 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein synthesis in archaebacteria and the cytoplasm of eukaryotes is initiated using the initiator methionyl-tRNA (Met-tRNA[subscript i][superscript Met]). In contrast, formylated methionyltRNA (fMet-tRNA[subscript i][superscript Met][subscript f]) is found in eubacteria, and in chloroplasts and mitochondria of eukaryotes, and this formylated initiator tRNA was widely believed to be required for initiation of protein synthesis in those systems. However, the fact that initiation of protein synthesis in yeast mitochondria can occur with unformylated initiator tRNA has changed our perspective about the initiation of mitochondrial protein synthesis. This dissertation is composed of two parts. Part I describes an investigation of the yeast AEP3 gene which was isolated by a genetic screening system in Saccharomyces cerevisiae. The main goal of this part was to discover new accessory factor(s) that might be involved in initiation of protein syntheis of yeast mitochondria when there is no formylation of initiator tRNA and determine how they support the initiation process in Saccharomyces cerevisiae. The synthetic petite genetic screen identified the AEP3 gene. Protein-protein binding assays as well as protein-initiator tRNA binding assays indicate that Aep3p is associated with the initiation process in yeast mitochondrial protein synthesis. This discovery is important because it suggests the possible mechanism by which initiation of protein synthesis in yeast mitochondria occur under conditions where there is no formylation of initiator tRNA. Part II describes a study of the TRM5 gene encoding a tRNA methyltransferase in S. cerevisiae. The TRM5 gene encodes a tRNA (guanine-N1- )-methyltransferase (Trm5p) previously known to methylate guanosine at position 37 (m1G37) in certain cytoplasmic tRNAs in S. cerevisiae. The main goal of this part was to investigate whether Trm5p is also responsible for m1G37 modification of mitochondrial tRNAs. Full-length Trm5p, purified as a fusion protein with maltose-binding protein, exhibited robust methyltransferase activity with tRNA isolated from a [Delta]trm5 mutant strain, as well as with a synthetic mitochondrial tRNA[superscript Met][subscript f] and tRNA[superscript Phe]. High pressure liquid chromatography analysis showed the methylated product to be m1G. Analysis of subcellular fractionation and immunoblotting revealed that the enzyme was localized to both cytoplasm and mitochondria. Our data including the analysis of N-terminal truncation mutants suggest that this tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis.

Download or read book Biochemistry and Genetics of Yeast written by Metry Bacila and published by Elsevier. This book was released on 2012-12-02 with total page 615 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry and Genetics of Yeasts: Pure and Applied Aspects consists of papers presented at a symposium organized by the Academia Brasileira de Ciencias held at the Universidade de Sao Paulo, on December 4-10, 1977. Organized into seven parts, this book reveals relevant and exciting developments in the areas of yeast genetics, respiration and fermentation, metabolic regulation, cell wall structure, synthesis of macromolecules, and transport. It demonstrates the presence of great progress in the knowledge of structure and functions of the yeast mitochondrial DNA. This book will be useful to scientific institutes and university laboratories interested in the biochemistry, genetics, and technology of yeasts.

Download or read book Genetic and Biochemical Analysis of the Mitochondrial Translation System in Saccharomyces Cerevisiae written by Zheng Cui and published by . This book was released on 1988 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mitochondria written by and published by Elsevier. This book was released on 2001-05-23 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies. - Isolation of mitochondria from commonly used cells and tissues - Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton - Biochemical and optical methods for studying protein-protein interactions in mitochondria - Approaches to studying mitochondrial replication, transcription, and translation - Transmitochondrial technologies - Methods in microassay data analysis

Download or read book Mitochondrial Function written by William S. Allison and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mitochondria written by Dario Leister and published by Springer Science & Business Media. This book was released on 2007-06-12 with total page 1195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Download or read book Search for Mutants of Mitochondrial Ribosomal Proteins in Saccharomyces Cerevisiae written by Zahra Salehi and published by . This book was released on 1982 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Guide to Yeast Genetics and Molecular and Cell Biology written by Christine Guthrie and published by Gulf Professional Publishing. This book was released on 2004 with total page 980 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Genetic Characteristization of MSH1 a Mitochondrial Protein of Saccharomyces Cerevisiae that Binds to DNA Mismatches written by Nai-Wen Chi and published by . This book was released on 1994 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Analysis of Human Mitochondrial Variants written by Noëlynn Anne Oliver and published by . This book was released on 1982 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mitochondrial Gene Expression written by and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mitochondria and Anaerobic Energy Metabolism in Eukaryotes written by William F. Martin and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-12-07 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondria are sometimes called the powerhouses of eukaryotic cells, because mitochondria are the site of ATP synthesis in the cell. ATP is the universal energy currency, it provides the power that runs all other life processes. Humans need oxygen to survive because of ATP synthesis in mitochondria. The sugars from our diet are converted to carbon dioxide in mitochondria in a process that requires oxygen. Just like a fire needs oxygen to burn, our mitochondria need oxygen to make ATP. From textbooks and popular literature one can easily get the impression that all mitochondria require oxygen. But that is not the case. There are many groups of organismsm known that make ATP in mitochondria without the help of oxygen. They have preserved biochemical relicts from the early evolution of eukaryotic cells, which took place during times in Earth history when there was hardly any oxygen avaiable, certainly not enough to breathe. How the anaerobic forms of mitochondria work, in which organisms they occur, and how the eukaryotic anaerobes that possess them fit into the larger picture of rising atmospheric oxygen during Earth history are the topic of this book.

Download or read book Yeast Protocols written by Wei Xiao and published by Humana. This book was released on 2016-09-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.

Download or read book Yeast Genetics written by Jeffrey S. Smith and published by Humana Press. This book was released on 2014-09-12 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Download or read book Yeast Sugar Metabolism written by Friedrich K. Zimmermann and published by CRC Press. This book was released on 1997-03-10 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.