Download or read book Genetic and Biochemical Analysis of Transcription in the Lac Control Region of Escherichia Coli written by Marguerite Amilie Sellitti and published by . This book was released on 1984 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Gene Expression in Escherichia coli written by E. C. C. Lin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.
Download or read book Genetic and Biochemical Analysis of the Colicin I Receptor Gene of Escherichia Coli written by David William Griggs and published by . This book was released on 1989 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cir gene, which encodes the colicin I receptor protein, is one of several outer membrane proteins synthesized by Escherichia coli in response to iron limitation. The location, organization, and regulation of cir and its neighboring genes were examined by a molecular genetic approach. Following cloning of the cir region, physical mapping and complementation analysis established the position of cir between mgl and nfo on the E. coli chromosome. A gene encoding a 32,000-dalton protein was located downstream of and adjacent to cir, was not part of the same transcriptional unit, and was not regulated by iron. The Cir protein was processed by removal of a signal peptide and the mature form had an amino terminal sequence which closely resembled that of other TonB-dependent proteins. The molecular basis for iron-regulated expression of cir was investigated by genetic and biochemical means. Deletion analysis of the cloned promoter region allowed delineation of sequences necessary for control of transcription initiating at the two promoters P1 and P2. Gel retardation assays were used to demonstrate a highly specific interaction between purified Fur (ferric uptake regulation) protein and the iron control region. An operator sequence spanning 43-47 base pairs and completely encompassing the two promoters was identified by DNase I protection experiments, with binding occurring in a metal-dependent fashion. Thus, during iron replete growth, Fur appears to act as a repressor of transcription by blocking formation of a DNA-RNA polymerase complex. Expression of cir in an miaA mutant was comparable to that in a wild type strain, so no ancillary role in iron-regulation based on the modification of the affected tRNA species was detected. Characterized and putative Fur recognition sites from several genes were analyzed and classified by statistical methods. In both high- and low-iron media, functional Crp protein and cyclic adenosine 3$spprime$,5$spprime$-monophosphate (cAMP) were required for maximal production of the receptor. A potential cAMP-CRP binding site located in the long leader region between the cir promoters and the start of the protein-coding region was suggested by sequence and mutational analysis.
Download or read book Genetic and Biochemical Studies on the Tryptophan Operon of Escherichia Coli written by Barbara Beckett Jones and published by . This book was released on 1975 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Maximizing Gene Expression written by William Reznikoff and published by Elsevier. This book was released on 2014-05-20 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.
Download or read book Genetic and Biochemical Analysis of the Escherichia Coli DNA Replication Origin written by Laurie Jo Simon LaVerne and published by . This book was released on 1980 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic and Biochemical Analysis of Escherichia Coli DNA Helicase II written by Brian Keith Washburn and published by . This book was released on 1992 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Function written by Robert E. Glass and published by Springer. This book was released on 2012-04-13 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: My aim in writing Gene Function has been to present an up-to-date picture ofthe molecular biology of Escherichia coli. I have not attempted a chronological description, believing that a mechanistic account is more useful for such a highly developed field. I have divided the book into four parts. Part I is a general introduction to bacterial systems, their genetic material, structure, composition and growth. It has seemed desirable to include herein a brief preview of the remaining text, to introduce the nomenclature and to help place subsequent chapters in perspective. The expression of genetic material and its perturbation through mutation is considered in Part II. Part III discusses how the transfer of prokaryotic genetic material can be mediated by plasmids and bacteriophages. It describes the DNA transactions involved (replication, recombination and repair) and ends with a description of the genetic and biochemical techniques employed in the study of gene organisation. Finally, Part IV considers the control of expression of bacterial, plasmid and phage genes. Key reviews are listed at the end of each chapter.
Download or read book Determination of the Nucleotide Sequence of the DNA Genetic Control Signals of the Lactose Operon of Escherichia Coli written by Wayne Morris Barnes and published by . This book was released on 1974 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcription Initiation at the Escherichia Coli Galactose Operon Promoter Region written by Sreenivasan Ponnambalam and published by . This book was released on 1988 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Quantitative Analysis of Genetic Transcriptional and Post transcriptional Regulation in Escherichia Coli written by Thomas Edward Kuhlman and published by . This book was released on 2007 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 4, Quantitative Characteristics of Gene Regulation by Small RNA, we study quantitatively two classes of bacterial small RNAs (sRNA) in Escherichia coli. We demonstrate that sRNA provide a novel mode of gene regulation with characteristics distinct from those of protein-mediated gene regulation. These include a threshold-linear response with a tuneable threshold and a built-in capability for hierarchical cross talk.
Download or read book Genetic and Biochemical Analysis of DNA Helicase II Mutants of Escherichia Coli written by Gang Zhang and published by . This book was released on 1997 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic and Biochemical Analysis of RNase E rne in Escherichia Coli K 12 written by Qi Liu and published by . This book was released on 1996 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The lac Operon written by Benno Müller-Hill and published by Walter de Gruyter. This book was released on 2011-05-12 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Operon written by Jeffrey H. Miller and published by . This book was released on 1980 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mims Medical Microbiology and immunology written by Richard Goering and published by Elsevier Health Sciences. This book was released on 2018-02-27 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn all the microbiology and basic immunology concepts you need to know for your courses and exams. Now fully revised and updated, Mims' clinically relevant, systems-based approach and abundant colour illustrations make this complex subject easy to understand and remember. - Learn about infections in the context of major body systems and understand why these are environments in which microbes can establish themselves, flourish, and give rise to pathologic changes. This systems-based approach to microbiology employs integrated and case-based teaching that places the 'bug parade' into a clinical context. - Effectively review for problem-based courses with the help of chapter introductions and 'Lessons in Microbiology' text boxes that highlight the clinical relevance of the material, offer easy access to key concepts, and provide valuable review tools. - Approach microbiology by body system or by pathogen through the accompanying electronic 'Pathogen Parade' – a quickly searchable, cross-referenced glossary of viruses, bacteria and fungi - A new electronic 'Vaccine Parade' offers quick-reference coverage of the most commonly used vaccines in current clinical practice - Deepen your understanding of epidemiology and the important role it plays in providing evidence-based identification of key risk factors for disease and targets for preventative medicine. - Grasp and retain vital concepts easily, with a user-friendly colour coded format, succinct text, key concept boxes, and dynamic illustrations. - New and enhanced information reflects the growing importance of the human microbiota and latest molecular approaches - Access the complete contents on the go via the accompanying interactive eBook, with a range of bonus materials to enhance learning and retention – includes self-assessment materials and clinical cases to check your understanding and aid exam preparation.
