Download or read book Genetic and Biochemical Analysis of the Mitochondrial Translation System in Saccharomyces Cerevisiae written by Zheng Cui and published by . This book was released on 1988 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Genetic Studies of Mitochondrial Protein Synthesis in Saccharomyces Cerevisiae written by Changkeun Lee and published by . This book was released on 2008 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein synthesis in archaebacteria and the cytoplasm of eukaryotes is initiated using the initiator methionyl-tRNA (Met-tRNA[subscript i][superscript Met]). In contrast, formylated methionyltRNA (fMet-tRNA[subscript i][superscript Met][subscript f]) is found in eubacteria, and in chloroplasts and mitochondria of eukaryotes, and this formylated initiator tRNA was widely believed to be required for initiation of protein synthesis in those systems. However, the fact that initiation of protein synthesis in yeast mitochondria can occur with unformylated initiator tRNA has changed our perspective about the initiation of mitochondrial protein synthesis. This dissertation is composed of two parts. Part I describes an investigation of the yeast AEP3 gene which was isolated by a genetic screening system in Saccharomyces cerevisiae. The main goal of this part was to discover new accessory factor(s) that might be involved in initiation of protein syntheis of yeast mitochondria when there is no formylation of initiator tRNA and determine how they support the initiation process in Saccharomyces cerevisiae. The synthetic petite genetic screen identified the AEP3 gene. Protein-protein binding assays as well as protein-initiator tRNA binding assays indicate that Aep3p is associated with the initiation process in yeast mitochondrial protein synthesis. This discovery is important because it suggests the possible mechanism by which initiation of protein synthesis in yeast mitochondria occur under conditions where there is no formylation of initiator tRNA. Part II describes a study of the TRM5 gene encoding a tRNA methyltransferase in S. cerevisiae. The TRM5 gene encodes a tRNA (guanine-N1- )-methyltransferase (Trm5p) previously known to methylate guanosine at position 37 (m1G37) in certain cytoplasmic tRNAs in S. cerevisiae. The main goal of this part was to investigate whether Trm5p is also responsible for m1G37 modification of mitochondrial tRNAs. Full-length Trm5p, purified as a fusion protein with maltose-binding protein, exhibited robust methyltransferase activity with tRNA isolated from a [Delta]trm5 mutant strain, as well as with a synthetic mitochondrial tRNA[superscript Met][subscript f] and tRNA[superscript Phe]. High pressure liquid chromatography analysis showed the methylated product to be m1G. Analysis of subcellular fractionation and immunoblotting revealed that the enzyme was localized to both cytoplasm and mitochondria. Our data including the analysis of N-terminal truncation mutants suggest that this tRNA modification plays an important role in reading frame maintenance in mitochondrial protein synthesis.

Download or read book Mitochondrial Biogenesis and Genetics written by Giuseppe Attardi and published by Gulf Professional Publishing. This book was released on 1996 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Structural and functional analysis of oxidative phosphorylation complexes * Import of proteins and RNA into mitochondria * Ion and metabolite transport systems in mitochondria * Biophysical methods for mitochondrial function analysis * Mitochondrial inheritance and turnover.

Download or read book Mitochondrial Gene Expression written by and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Search for Mutants of Mitochondrial Ribosomal Proteins in Saccharomyces Cerevisiae written by Zahra Salehi and published by . This book was released on 1982 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of Saccharomyces written by L.A. Grivell and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: At a fundamental research level, the yeasts offer valuable opportunities for modelling regulatory and metabolic processes in multicellular eukaryotic organisms: this volume deals with the multifunctional chromosome regulatory proteins, topoisomerase and nuclear transport. A combination of biochemical and genetic approaches applied to the yeast translation system is also currently yielding a wealth of data, while the mating pheromone signal transduction pathway in yeasts provides a valuable analogue of the signal transduction components used by multicellular organisms, including receptors, G proteins, protein kinases and transcription factors. With a well-established history of fermantation studies, yeasts remain the first-choice vehicle for production of heterologous eukaryotic proteins. Interest is diversifying, as an increasing number of non-Saccharomyces species are now being utilised for the production of specific heterologous proteins. Molecular biologists, microbiologists and biochemical geneticists will find this volume an authoritative and valuable update on a vibrant area of research.

Download or read book Genetic and Biochemical Studies of Mitochondria in the Yeast Saccharomyces Cerevisiae written by K. Allen G. Donald and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Synthesis and Targeting in Yeast written by Alistair J.P. Brown and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to fundamental similarities between the yeast Saccharomyces cerevisiae and multicellular organisms at the molecular level, and the powerful range of experimental tools available for this yeast, S. cerevisiae is proving an ideal model system for studies on protein synthesis and targeting. The topics covered are: - Messenger RNA stability and translation.- The translation apparatus. - Translational control andfidelity. - Protein targeting to the mitochondrion. - Nuclear transport. - The secretory pathway. - Protein folding and degradation. - Protein splicing. Modern and often novel molecular, genetic and biochemical approaches as well as most recent data are provided. The reader will gain a comprehensive view of the current status of the field.

Download or read book A Genetic and Biochemical Analysis of Pre mRNA Splicing in Saccharomyces Cerevisiae written by Usha Vijayraghavan and published by . This book was released on 1989 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comparative genetic and mutational analysis of the translation inititation process in Saccharomyces cerevisiae written by Andrew Mark Cigan and published by . This book was released on 1988 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mitochondrial Function written by William S. Allison and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Genetic Characteristization of MSH1 a Mitochondrial Protein of Saccharomyces Cerevisiae that Binds to DNA Mismatches written by Nai-Wen Chi and published by . This book was released on 1994 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular and Genetic Analysis of Mitochondrial Protein Localization in Saccharomyces Cerevisiae written by Lori Kay Dircks and published by . This book was released on 1991 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Analyses of Thialysine Resistant Mutants of Saccharomyces Cerevisiae written by John H. Zwolshen and published by . This book was released on 1978 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Studies of a Mutant Saccharomyces Cerevisiae Myristoyl CoA written by D. Russell Johnson and published by . This book was released on 1993 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biochemical and Genetic Analysis of Mot1 a Regulator of Basal Transcription Saccharomyces Cerevisiae written by Joanne Irene Adamkewicz and published by . This book was released on 1999 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Studies of the Group II Intron AI2 RNP Particle in the Mitochondria of the Bakers Yeast Saccharomyces Cerevisiae written by Michael Yi Chao and published by . This book was released on 2001 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: