Download or read book Genetic and Biochemical Analyses of the DNA Damage Response Pathway in Yeast Saccharomyces Cerevisiae written by Zheng Zhou and published by . This book was released on 1994 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Genetic and Molecular Analyses of DUN2 POL2 and DUN5 Their Roles in the DNA Damage Response Pathway in Saccharomyces Cerevisiae written by Tony A. Navas and published by . This book was released on 1996 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Damage Response in Live Yeast Using Single molecule Microscopy written by Shubhika Ranjan and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "The genetic information stored in DNA must be faithfully copied and transmitted to the next generation of cells at every cell cycle. The replication of this genetic information is performed by special multiprotein replication machinery, referred to as "replisome," which synthesizes both daughter duplexes simultaneously. At times, the accurate replication of the genome can go awry, causing mutations that can lead to a collection of diseases. In eukaryotic cells, detection and response to DNA damage during DNA replication is performed by the DNA damage response (DDR) pathway. DDR uses exposed single-stranded DNA as a signal for DNA damage and proceeds by activation of kinases that transmit the signal and change the cell program to respond to the damage. The overall purpose of this work is to understand how cells do this initial detection and how they make the decision to activate the signalling pathway. I used budding yeast (Saccharomyces cerevisiae), a unicellular model organism, to understand the eukaryotic genetic architecture because it provides a framework to develop and optimize methods to standardize the analysis. I focus on the study of dynamics of DNA replication and DDR proteins of cells experiencing DNA damage or replication fork stalling using single-molecule microscopy. Although this technique provides a high signal-to-noise ratio for visualization while still retaining the integral features in the physiological context of biological systems, various factors play a major role in attaining such high-quality data for further analysis. In the first part of this work, I provide an overview for optimizing the single-molecule techniques while considering various factors involved. In the second section, I describe the initial work towards visualizing the proteins involved in DNA damage response in HaloTag labelled S. cerevisiae"--
Download or read book Biochemical Analysis of Saccharomyces Cerevisiae Mus81 Mms4 an Endonuclease that Supports Eukaryotic DNA Replication written by Kirk Tevebaugh Ehmsen and published by . This book was released on 2008 with total page 938 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemical and Genetic Analysis of Excision DNA Repair written by Johnson Man Su Wong and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The integrity of the genetic material is constantly threatened by various DNA damaging agents. Hence, multiple DNA repair pathways have evolved to eliminate damage of different origins. Nucleotide excision repair (NER) is the primary mechanism by which both 'S. cerevisiae' and human cells remove lesions, including those induced by ultraviolet light, that usually cause severe distortions of the DNA helix. I have described a simple protocol for preparing yeast whole cell extracts that can support efficient NER to facilitate biochemical studies of this repair pathway in yeast. This assay reflects ' bona fide' NER as it depends on 'RAD' genes such as ' RAD14' and 'RAD2', which are known to be essential for excision repair. Interestingly, the assay was also dependent on the ' RAD7' and 'RAD16' genes, whose precise roles in NER are uncertain. Using this system, I also demonstrated that yeast replication protein A (Rpa), encoded by the 'rfa'2 gene, is required for NER, since 'rfa2' mutations which confer enhanced UV sensitivity ' in vivo' also resulted in a deficiency in NER 'in vitro'. NER can be divided into two subpathways: global genome repair, which is involved in the repair of both active and inactive genes, and transcription-coupled repair. In the latter subpathway, lesions in the transcribed strands of active genes are more rapidly repaired, but contributions of the transcription machinery to this repair pathway are poorly understood. To determine whether the RNA polymerase elongation factor SII plays a role in excision repair, I examined the effect of deleting the SII gene of 'Saccharomyces cerevisiae'. Lack of SII activity enhanced UV sensitivity, but only in the absence of ' RAD7/16'-dependent global genome repair. This increased UV sensitivity was also seen with RNA polymerase II mutants defective in their response to SII. UV sensitivity was also conferred by other RNA polymerase II mutations that do not affect SII function. Another pathway of excision repair, base excision repair (BER), involves DNA glycosylases that recognize and excise specific types of base damage in DNA, including uracil and thymine glycol. Human uracil DNA glycosylase (UDG) was recently found to interact with the 34 kDa subunit of human RPA. To investigate whether RPA plays a role in BER in yeast and human cells, I set up ' in vitro' BER systems using yeast and HeLa cell extracts. Both of these systems appear capable of performing efficient BER in the presence of several types of lesions. I fractionated HeLa extracts to deplete endogenous RPA and I will test the ability of these fractions to perform BER. (Abstract shortened by UMI.).
Download or read book A High throughput Screen for Novel Genes Involved in Maintaining Genome Stability and the DNA Damage Response Pathway in Saccharomyces Cerevisiae written by Jason Alexander Hendry and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Recombination and Repair written by Paul James Smith and published by Oxford University Press, USA. This book was released on 1999 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The processes of DNA recombination and repair are vital to cell integrity - an error can lead to disease such as cancer. It is therefore a large and exciting area of research and is also taught on postgraduate and undergraduate courses. This book is not a comprehensive view of the field, but a selection of the issues currently at the forefront of knowledge.
Download or read book Yeast Functional Genomics written by Frédéric Devaux and published by Humana. This book was released on 2015-10-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book DNA Damage Recognition written by Wolfram Siede and published by CRC Press. This book was released on 2005-09-19 with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stands as the most comprehensive guide to the subject-covering every essential topic related to DNA damage identification and repair. Covering a wide array of topics from bacteria to human cells, this book summarizes recent developments in DNA damage repair and recognition while providing timely reviews on the molecular mechanisms employe
Download or read book DNA Damage and Repair written by A. Castellani and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First International Congress on DNA Damage and Repair was held in Rome, Italy, July 12-17, 1987. It was organized by the Italian Com mission for Nuclear Alternative Energy Sources. The subject of DNA damage and repair involves almost all the fields ofbidogical sciences. Some of the more prominent ones include carcino genesis, photobiology, radiation biology, aging, enzymology, genetics, and molecular biology. These individual fields have their own interna tional meetings and although the meetings often have sessions devoted to DNA repair, they do not bring together a wide diversity of international workers in the field to exchange ideas. The purpose of the Congress was to facilitate such an exchange among scientists representing many fields of endeavor and many countries. The 37 manuscripts in this volume, presented by the invited spea kers during the four and half days of the Congress, encompass the field of DNA damage and repair. They cover biological systems ranging from mo lecules to humans and deal with damages and repair after treatment of cells with various types of radiations, chemicals, and exogenous and en dogenous oxidative damages. The Congress and its Proceedings are dedicated to two international leaders in the field of DNA damage and repair, Alexander Hollaender of the United States and Adriano Buzzati Traverso of Italy. Hollaender, who died in December 1986, was one of the first investigators to recognize the damage to DNA was important in cell killing and mutagenesis. His early work indicated that cells could recover from radiation injury.
Download or read book Stress Induced Mutagenesis written by David Mittelman and published by Springer Science & Business Media. This book was released on 2013-03-12 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery of stress-induced mutagenesis has changed ideas about mutation and evolution, and revealed mutagenic programs that differ from standard spontaneous mutagenesis in rapidly proliferating cells. The stress-induced mutations occur during growth-limiting stress, and can include adaptive mutations that allow growth in the otherwise growth-limiting environment. The stress responses increase mutagenesis specifically when cells are maladapted to their environments, i.e. are stressed, potentially accelerating evolution then. The mutation mechanism also includes temporary suspension of post-synthesis mismatch repair, resembling mutagenesis characteristic of some cancers. Stress-induced mutation mechanisms may provide important models for genome instability underlying some cancers and genetic diseases, resistance to chemotherapeutic and antibiotic drugs, pathogenicity of microbes, and many other important evolutionary processes. This book covers pathways of stress-induced mutagenesis in all systems. The principle focus is mammalian systems, but much of what is known of these pathways comes from non-mammalian systems.
Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.
Download or read book The Molecular Biology of the Yeast Saccharomyces Life Cycle and Inheritance written by Jeffrey N. Strathern and published by . This book was released on 1981 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metabolism and Molecular Physiology of Saccharomyces Cerevisiae written by J. Richard Dickinson and published by CRC Press. This book was released on 2004-04-27 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and r
Download or read book Non Conventional Yeasts in Genetics Biochemistry and Biotechnology written by Klaus Wolf and published by Springer Science & Business Media. This book was released on 2003-03-04 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Download or read book Molecular Biology of the Fission Yeast written by and published by Elsevier. This book was released on 2012-12-02 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism.
Download or read book Yeast Genetics written by Jeffrey S. Smith and published by Humana Press. This book was released on 2014-09-12 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
