Download or read book Genetic Analysis of Tumor Progression in the Mouse Mammary Gland written by Nathan Frederick Schachter and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic alterations that cooperate with TP53 mutation in breast cancer (BC) are largely unknown. Using a dominant-negative Trp53LSL-R270H/+ mouse strain, I found Trp53 alteration cooperates with Her2/Neu over-expression, but not with hemizygous deletion of Gata3, Runx1, or Nf1. To identify other genes cooperating with mutant Trp53, I completed a Sleeping Beauty (SB) mutagenesis screen in mice. This identified known cooperators such as Met, and other candidates such as Notch1. Cooperation between activated Notch1 and Trp53LSL-R270H/+ was validated in vivo. In addition to coding mutations, chromosomal copy number changes are also important in BC. To identify genes that contribute to mammary tumorigenesis upon copy number alteration, I compared genes targeted by transposons in SB mice carrying Trp53LSL-R270H/+, R26LSL-Pik3ca(Mut)/+, R26LSL-Stat3C/+, or no mutant allele. This enabled me to identify genes altered in all cohorts (e.g. Rasa1), in several but not all backgrounds (e.g. Met), and those that only occurred in the presence of a single driver (e.g. Mll3, Exoc4, and Cul1 loss in Pik3ca-mutant tumors). Importantly, many genes targeted by SB transposons map to loci syntenic with regions commonly gained/lost in human BC. Thus, they likely represent haploinsufficient tumor suppressors or oncogenes. Guided by SB results, the oncogenic interaction between R26LSL-Pik3ca(Mut)/+ and loss/activation of Notch1 was validated in vivo. Additionally, I distinguished genes altered in metastatic lesions from SB mice. This revealed genes previously implicated in BC dissemination (e.g. Hipk2) and novel abnormalities (e.g. loss of Fbxl17). Lastly, to assess the oncogenic potential of ELF3 (which maps to human chromosome 1q and is frequently gained/amplified in human BC), I generated a Cre-conditional Rosa26-targeted Elf3 transgenic strain (R26LSL-Elf3). Using SB mutagenesis, I showed genes such as Cul5 and FoxP1 were disrupted in Elf3 over-expressing SB mammary tumors, while metastases from these mice selected for a distinct set of insertions (e.g. Pard3 disruption).
Download or read book Perspectives in Mammary Gland Development and Breast Cancer Research written by Zuzana Koledova and published by Frontiers Media SA. This book was released on 2020-10-27 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Download or read book Genetically Engineered Mice for Cancer Research written by Jeffrey E. Green and published by Springer Science & Business Media. This book was released on 2011-12-09 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetically-engineered mouse models for cancer research have become invaluable tools for studying cancer biology and evaluating novel therapeutic approaches. This volume focuses on state-of-the-art methods for generating, analyzing and validating such models for studying aspects of human cancer biology. Additionally, these models are emerging as important pre-clinical systems in which to test cancer prevention and therapeutic strategies in order to select compounds for testing in clinical trials.
Download or read book Methods in Mammary Gland Biology and Breast Cancer Research written by Margot M. Ip and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: approaches to the experimental problems that still face us in understanding this most fascinating of organs. Too many people contributed to the completion of this volume to allow acknowledg ment of all the individual efforts, but we particularly thank the reviewers whose input into the editorial process was invaluable and the authors of these chapters who revised their text, sometimes more than once, to bring it to the high standards set by the Editors. The Com mittee gratefully acknowledges the support ofVysis, Inc. , in the publication of a color figure in Chapter 19, by S. Weber-Hall and Trevor Dale. Finally, we wish to express our heartfelt appreciation to Margot Ip and Bonnie Asch, who worked long and hard to bring this volume to fruition. Margaret C. Neville for the Committee on Mammary Gland Biology Preface One of the most exciting and beneficial developments in research on mammary gland biology and breast cancer has been the influx of increased funding to support this work. This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi tional money from various federal and state sources, has led to a rapid expansion of research efforts by attracting numerous new investigators into the field. These new investigators include students, postdoctoral fellows, and scientists from other fields.
Download or read book A Genetic Analysis of the Factors Underlying Susceptibility to Transplantable Tumors written by Leonell Clarence Strong and published by . This book was released on 1922 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biology of the Mammary Gland written by John A. Mol and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the European Cooperation in the Field of Scientific and Technical Research (COST 825) Symposium on Mammary Gland Biology, held September 16-18, 1999, in Tours, France. It is difficult to overstate the evolutionary and functional significance of mammary tissue in biology. Substantial progress has been made by researchers in various disciplines, particularly over the last fifteen years, towards realizing the potential of this tissue to yield powerful experimental models for morphogenesis and tissue development; for cellular differentiation; for the biosynthesis and secretion of proteins, lipids, small molecules and inorganic salts; and for the coordination and regulation of these processes. More recently, the possibility of exploiting the secretory epithelial cells of mammary tissue as `cell factories' has become a reality and the recombinant production by lactating animals of an increasing number of proteins, valuable both in the pharmaceutical and `nutraceutical' fields, is in progress or under development. Also in this sphere of agricultural production, genetic as well as nutritional technologies are under investigation and exploitation to optimize milk composition for various end-uses - for instance in food process and manufacture. The possibilities of deriving health benefit from the bioactive properties of some of the minor constituents of milk are emerging to counter the highly-publicized negative health impact of excessive consumption of saturated animal fats. In human nutrition and medicine, the mammary gland is both a source of nutrition to the neonate and a potential health threat to the adult female - breast cancer remains the major single cause of female mortality in most developed countries. This volume provides a unique glimpse into our understanding, at the cutting edge of a variety of disciplines, of this versatile and extraordinary tissue, at the birth of the twenty-first century.
Download or read book Gene Expression Profiling of Mammary Gland Tumors from Transgenic Mice written by Alexander Matthew Lehrman and published by . This book was released on 2006 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Salivary and Mammary Gland Tumorigenesis in PLAG1 Transgenic Mice written by Isabella C. C. van Valckenborgh and published by Leuven University Press. This book was released on 2005 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a Ph.D. dissertation. Contents include: Introduction: The normal salivary gland, Salivary gland tumors, Pleomorphic adenoma of the salivary glands, PLAG1, Unraveling human cancer using the mouse as a model system, Salivary gland transgenesis; Aims and Rationale; Material and Methods: Immunohistochemistry, Cytogenetic analysis, FISH analysis, Immunoflourescence, Cloning strategies, DNA sequencing, Transfections and luciferase assay, Western blot analysis, Generation of transgenic mouse strain by zygote micro-injection, PCR analysis, Isolation of RNA, Northern blot analysis, Isolation of DNA, Southern blot analysis, Labeling of DNA probes, Histology, Gene expression profiling; Results: Immunohistological localization studies of PLAG1 in human pleomorphic adenoma of the salivary gland, Human PLAG1 transgenic mouse strain, Study of PLAG1 and PLAGL2 target genes by microarray analysis; Discussion and Perspectives: Immunohistological localization studies of PLAG1 in the human pleomorphic adenoma of the salivary gland, Human PLAG1 transgenic mouse strain, Study of PLAG1 and PLAGL2 target genes by microarray analysis; General conclusions; Summary; References.
Download or read book Genetic Analysis of Noncanonical Smoothened Function in the Mouse Mammary Gland written by and published by . This book was released on 2015 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of a Transgenic Mammary Gland System for the Study of Mammary Tumorigenesis written by Carlos Estuardo Aguilar-Cordova and published by . This book was released on 1989 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Breast Cancer written by Anne M. Bowcock and published by Springer Science & Business Media. This book was released on 1999-01-11 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art summary of breast cancer research and treatment by leading authorities. The book's many distinguished contributors illuminate the biology and genetics of breast cancer, including what is known about the hereditary breast cancer genes, BRCA1 and 2, the cutting-edge cytogenic approaches, and the biology of breast cancer metastasis. In addition, the authors describe current and future methods of breast cancer treatment in depth, and discuss environment and diet as risk factors for the disease. Breast Cancer: Molecular Genetics, Pathogenesis, and Therapeutics constitutes an excellent reference and resource for all those clinical and experimental oncologists, as well as genetic counselors nurses, who need to understand the latest developments in breast cancer biology, risk, and treatment.
Download or read book In Vivo Characterization of Mammalian Polarity Genes as Novel Tumor Suppressors Involved in Breast Cancer Development and Progression in a Mouse Model written by and published by . This book was released on 2006 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of our study is to understand the role of novel polarity regulators mammary gland development and their ability to cooperate with oncogenes in tumorogenesis within this gland. We are using mouse systems as well as an analysis of cell lines to understand the role of a particular gene, Scribble, in this process. So far, I have been able to identify one human breast cancer cell line with little scribble expression. In a normal mouse cell line, comma-1D, we are doing further analysis as to the effects of scribble loss using RNAi technology. We have observed a morphological change, with a loss of e-cadherin, as well as a mild proliferative change in scribble knockdown cells. We have also observed a change in lineage specific cytokeratins in these cells. This data is significant as it possibly demonstrates the role of a polarity gene the differentiation of the breast as well as in tumors with concomitant growth changes to the affected tissue.
Download or read book Cellular and Molecular Biology of Mammary Cancer written by E. Anderson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for this book arose during the 1985 Gordon Conference on "Mammary Gland Biology". New developments in the methodology of cell biology and the explosive growth of molecular biology had begun to impact upon our understanding of mammary gland growth and function. It seemed a propitious time for summarizing the current status of knowledge of the cell and molecular biology of mammary cancer and for attempting to outline future areas of concern and interest. The reviews presented here were completed by the Fall of 1986. Although new insights will surely continue to emerge, it is hoped that the material in this volume will form not only a current update but a basic core of information for future experiments. We have not attempted to cover all areas of mammary gland transformation. Those areas where recent detailed reviews are already available have been omitted. Also, the areas of normal gland development, cell ultrastructure, hormone responsiveness, chemotherapy and clinical aspects of mammary cancer have not been included. Instead, we have selected those areas where the development of new methodology, reagents and results have led to new ideas about mammary gland function and development as they are related to neoplasia.
Download or read book A Symposium on Mammary Tumors in Mice written by National Cancer Institute (U.S.) and published by . This book was released on 1945 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mouse Models of Human Cancer written by Eric C. Holland and published by John Wiley & Sons. This book was released on 2004-08-27 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mice have become the species of choice for modeling the complex interactions between tumor cells and the host environment. Mouse genetics are easily manipulated, and a growing array of technology exists for this purpose. Mouse models allow investigators to better understand causal relationships between specific genetic alterations and tumors, utilize new imaging techniques, and test novel therapies. Recent developments along these lines show great promise for the development of new anti-cancer treatments. Mouse Models of Human Cancer provides researchers and students with a complete resource on the subject, systematically presenting the principles, methodologies, applications, and challenges associated with this exciting field. Offering a survey of the latest research and a description of future areas of interest, this text: Presents real experimental data Describes organ site-specific mouse models Clearly identifies suitable models for further drug testing Critically analyzes current methodologies and their limitations Features numerous recognizable expert contributors Lists key Web sites, reagents, and companies From mouse handling and genetic engineering to preclinical trials, Mouse Models of Human Cancer is a comprehensive guide to using these models and relating them to human disease. Its uniform presentation describes organ-specific models in clinical, imaging, and molecular terms, and lays out the relevant genetics, experimental approaches, histological comparisons with human disease, and conclusions. Combining stellar chapter authors, rich illustrations, and clear, up-to-date coverage, Mouse Models of Human Cancer is an invaluable resource for advanced students and cutting-edge researchers.
Download or read book The Role of GATA 3 in the Mammary Gland and in Breast Cancer written by Hosein Kouros-Mehr and published by . This book was released on 2006 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis, I demonstrate that the transcription factor GATA-3 plays a critical role in the development and homeostasis of the mammary gland and in the malignant conversion of breast cancer. I show that GATA-3 is the most abundant transcription factor in the mammary epithelium of mice and that its normal function is to establish and maintain the differentiation of luminal epithelium. I then show in a mouse model of breast cancer (MMTV-PyMT) that the loss of GATA-3 is a critical event during malignant progression. That is, loss of GATA-3 marks the transition from adenoma to carcinoma and the onset of tumor dissemination to distant sites. I show that the reintroduction of GATA-3 in metastatic adenocarcinomas is sufficient to induce tumor differentiation and to significantly inhibit tumor dissemination. In addition to GATA-3, I identify through microarray analysis a number of genes that may regulate branching morphogenesis of the mammary gland. This includes specific members of the Wnt, hedgehog, ephrin, and EGFR ligand families.
Download or read book Mammary Tumors in the Mouse written by Jo Hilgers and published by Elsevier-North-Holland Biomedical Press. This book was released on 1981 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt:
