Download or read book Genetic Analysis of the U5 SnRNA and the 3 Splice Site in Yeast written by Bruce Patterson and published by . This book was released on 1989 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Analysis of Second step Pre mRNA Splicing Facmtors in Saccharomyces Cerevisiae written by Daniel N. Frank and published by . This book was released on 1993 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcription and Splicing written by B. D. Hames and published by Oxford University Press, USA. This book was released on 1988 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a co-ordinated review of our present knowledge of eukaryotic RNA synthesis.
Download or read book RNA and Protein Interactions in the Yeast Spliceosome written by Deming Xu and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear pre-mRNA splicing proceeds in a protein-RNA complex, the spliceosome, via a two-step transesterification reaction. Five spliceosomal small nuclear RNAs; (snRNAs) are brought to the pre-mRNA substrate in a process known as spliceosome assembly. Extensive RNA-RNA interactions and conformational changes occur during spliceosome assembly and maturation. However, very little is known about how RNA conformational changes are coordinated between the two steps of the splicing reaction, and how recognition of the 3' splice site by other factors is connected to the function of U5 loop 1 prior to and following the first splicing step. I performed a genetic screen in order to identify factors that are important for the U2/U6 helix II interaction and/or the function of the 5' -end of U2 snRNA, on the basis of synthetic lethality with an 11nt nucleotide substitution in the 5'-end region of U2 snRNA that could also perturb the U2/U6 helix II interaction. Six Slt (synthetic lethal with U2 snRNA) factors were isolated in this screen. Slt11p and Slt22p are new splicing factors, while the remainder correspond to previously identified splicing factors, Slt15p/Prp17p, Slt16p/Smd3p, Slt17p/Slu7p and Slt21p/Prp8p. Slt22p is a large RNA-dependent ATPase, whose activity is preferentially stimulated by pre-annealed U2/U6 snRNAs. Biochemical and genetic analyses indicated that its function is associated with U2/U6 snRNA interaction and that it may unwind intermolecular helix II prior to the formation of the active spliceosome. Slt2lp/Prp8p has been shown to be required for both steps of the splicing reaction. The 'slt'21/'prp'8-21 mutation has been mapped to a domain in Prp8p that is important for the recognition of the polypyrimidine tract preceding the 3' splice site. It is synthetically lethal with mutations in only the U2 part of U2/U6 helix II. Consistent with the notion that the 5'-end of U2 snRNA plays a role in the second splicing step, I found that U2 mutations in this region are synthetically lethal with several second-step mutations, including 'slt15'/'prp17-100', ' slu4'/'prp17-2', 'slt17'/' slu7-100', and 'slu7-1'. Slt11p is a putative RNA-binding protein containing two Zn-finger motifs. Although 'SLT11' is essential for viability only at elevated temperatures, its product is required for the efficiency of the splicing reaction. In the absence of Slt11p, 'slt17'/'slu7-100' and mutations in specific regions of U2, U5 and U6 snRNAs become lethal. (Abstract shortened by UMI.).
Download or read book A Genetic Analysis of 5 Splice Site Selection in Saccharomyces Cerevisiae written by Cammie F. Lesser and published by . This book was released on 1993 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spliceosomal Pre mRNA Splicing written by Klemens J. Hertel and published by Humana. This book was released on 2014-02-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.
Download or read book Molecular Biology of Saccharomyces written by L.A. Grivell and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: At a fundamental research level, the yeasts offer valuable opportunities for modelling regulatory and metabolic processes in multicellular eukaryotic organisms: this volume deals with the multifunctional chromosome regulatory proteins, topoisomerase and nuclear transport. A combination of biochemical and genetic approaches applied to the yeast translation system is also currently yielding a wealth of data, while the mating pheromone signal transduction pathway in yeasts provides a valuable analogue of the signal transduction components used by multicellular organisms, including receptors, G proteins, protein kinases and transcription factors. With a well-established history of fermantation studies, yeasts remain the first-choice vehicle for production of heterologous eukaryotic proteins. Interest is diversifying, as an increasing number of non-Saccharomyces species are now being utilised for the production of specific heterologous proteins. Molecular biologists, microbiologists and biochemical geneticists will find this volume an authoritative and valuable update on a vibrant area of research.
Download or read book Advances in Genetics written by Jeffrey C. Hall and published by Elsevier. This book was released on 1994-05-27 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Under the leadership of Dr. Jeffrey C. Hall and Dr. Jay C. Dunlap, Advances in Genetics covers all genetic systems-from prokaryotic to human-with an eye toward identifying emerging problems as they coalesce. Volumes present both synoptic and topical reviews in a comprehensible, informative, and insightful manner. Articles range from detailed and discursive to brief and sharply focused, as benefits the problem under study. Founded in 1946 by Dr. Miloslav Demerc, this serial publication continues to be critical reading for researchers who need current overviews of areas outside their specialty and for students who need comprehensive introduction to topics genetic research. Under new editorial direction beginning with this volume Publishes comprehensive and timely chapters of genetic Delves into genetic phenomena at a molecular level Ties together related issues in cell and developmental biology and neurobiology
Download or read book Genetic Analysis of the Yeast Transfer RNA splicing Endonuclease and Its Substrates written by Mark Edward Winey and published by . This book was released on 1988 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Role of Protein RNA Interactions in 3 Splice Site Selection During Pre mRNA Splicing written by James G. Umen and published by . This book was released on 1995 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Alternative Splicing and Disease written by Philippe Jeanteur and published by Springer Science & Business Media. This book was released on 2006-10-04 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: Splicing of primary RNA transcript is a quasi-systematic step of gene expression in higher organisms. This is the first book to highlight the medical implications, i.e. diseases, caused by alternative splicing. Alternative splicing not only vastly increases protein diversity but also offers numerous opportunities for aberrant splicing events with pathological consequences. The book also outlines possible targets for therapy.
Download or read book Alternative Splicing in the Postgenomic Era written by Benjamin J. Blencowe and published by Springer Science & Business Media. This book was released on 2008-01-23 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yet again Springer has reached the market before everyone. This is the first book that is solely dedicated to the topic of alternative splicing. The book contains chapters by experts in the field that cover nearly all aspects of this hugely important subject. The purpose of the text is to provide a single, authoritative source of information on alternative splicing that is accessible to researchers in diverse fields. It is suitable for beginners and experts alike.
Download or read book Eukaryotic MRNA Processing written by Adrian Krainer and published by IRL Press. This book was released on 1997 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
Download or read book Regulation of Pre mrna Splicing Efficiency and Fidelity in Saccharomyces Cerevisiae written by Katherine Anne Senn and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pre-mRNA splicing, the removal of noncoding introns to form a protein coding mRNA, is a crucial step in eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five small nuclear RNAs (snRNAs) and dozens of proteins organized into small nuclear ribonucleoproteins (snRNPs), catalyzes splicing in two steps. These steps are highly regulated to ensure correct mRNA isoform production and protein expression. Core components of the spliceosome as well as additional auxiliary factors tune splicing for accuracy and regulation of alternative splicing outcomes. Because of the complexity of splicing regulation, there are many open questions about the factors involved and the regulation of alternative splicing. This thesis addresses the functions of two yeast proteins involved in splicing regulation and an alternatively spliced gene in humans.In chapters 2 and 3, I discuss the novel yeast splicing factor Fyv6. Genetic and biochemical evidence supports a role for Fyv6 in the second step of splicing, exon ligation. A collaborator has determined the structure of a Fyv6-containing spliceosome by cryo-electron microscopy. Using this structure, we have used biochemical and transcriptomics experiments to elucidate how Fyv6 affects 3' splice site choice. Yeast splicing regulatory factor Mer1 interacts with introns and the U1 snRNP to enhance splicing of a subset of meiotically-regulated pre-mRNAs by an as-yet undetermined mechanism. With the goal of elucidating the mechanism, I recapitulated Mer1-dependent splicing in vitro and used colocalization single molecule microscopy to examine how Mer1 affects the dynamics of U1 binding to pre-mRNAs (Chapter 4). Our results indicate Mer1 promotes splicing without significantly altering the observed distributions of U1 binding events. This study lays the groundwork for future studies of splicing regulation by Mer1 and other factors. Finally, I studied the expression and alternative splicing of human glucose transporter family member GLUT8 (Chapter 5). Multiple alternatively spliced GLUT8 mRNA isoforms exist, but only some encode viable proteins. One that does not is upregulated in cancers, which reduces GLUT8 protein expression. Collaborators showed that based on its localization, inability to transport glucose, and release of a cleaved peptide, the main functional isoform of GLUT8 may be a metabolic sensor.
Download or read book Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles written by Max L. Birnstiel and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade a most interesting story of the role played by the small nuclear RNPs, catalysts in RNA processing, has unfolded. Early investigations of the structure of these particles gave rise to hypotheses of their functions. As described in this book, these have been confirmed or are being tested by biochemical ex perimentation and most recently by the powerful technique of genetic analysis. Both biochemical and genetical approaches will also determine to what degree, and how, snRNPs and their cofactors participate in the differential expression of genes. As the details emerge and the number of snRNP species increases way beyond the six initially identified, one feels at the threshold of even greater things to come. The book covers many results in one of the most rapidly expanding fields of molecular biology. For this reason alone the specialist may detect some omissions and shortcomings. I hope they will be few. Instead of presenting a collation of conference reports with much overlap between them, this book has been written specially for the purpose of surveying the literature up to early 1987 in the snRNP field in a coherent manner, each of the seven chapters having been produced by connoisseurs of their field. While not each and every snRNP story can be covered in such a book, I hope that it will provide enjoyable and stimulating reading.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2001 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Biochemistry and Biotechnology written by Jan Barciszewski and published by Springer Science & Business Media. This book was released on 1999-08-31 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Biochemistry and Biotechnology describes various aspects of nucleic acid and protein structure, mainly RNA structure and proteins, interacting with specific RNA species. Papers deal with DNA protein interactions, telomerase, aminoacyl-tRNA synthetases, elongation factor Tu, DNA repair, RNA structure, NMR technology, RNA aptamer interaction of biological macromolecules with metal ions. Two papers deal with theoretical aspects of RNA structure production and computer modelling. Many papers describe the possibility of commercial application of RNA biotechnology. One article discusses the impact of direct democracy on basic science supporting biotechnology. Readership: Advanced graduate students, Ph.D. students and young scientists as well as specialists in the field.
