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Book Phenotypic Characterization of Escherichia Coli Strains Taken from Human Intestinal and Urinary Tracts

Download or read book Phenotypic Characterization of Escherichia Coli Strains Taken from Human Intestinal and Urinary Tracts written by Kruthi Murthy and published by . This book was released on 2006 with total page 79 pages. Available in PDF, EPUB and Kindle. Book excerpt: Escherichia coli (E. coli) is a gram negative bacterium commonly found as a commensal in the intestinal tract of humans and other warm blooded animals. The commensal strains of E. coli are non-pathogenic and do not cause an infection in the host. However, some strains of E. coli are pathogenic and can cause several diseases in humans that include neonatal meningitis, intestinal infections and urinary tract infections. Almost 80-85% of the uncomplicated urinary tract infections are caused by uropathogenic E. coli. Our project involved the characterization of 12 strains of E. coli, isolated from humans in health and disease. These strains were characterized since no studies had been conducted on them previously. The E. coli strains were isolated from patients with urinary tract infections and from the intestinal tracts of partners of these patients. Laboratory E. coli strain K12 served as a control. Phenotypic studies were carried out by studying the bacterial physiology in three different conditions. Motility tests were done to identify phenotypes that were similar to and different in behavior with respect to the wild type. Since antimicrobial resistance was a growing problem in urinary tract infections, susceptibility tests were done for these novel strains of E. coli, with the most commonly used antibiotics to treat these infections. Additionally phenotypic profiles were generated for the wild type strain as well as one uropathogenic strain using Phenotype Microarrays manufactured by Biolog., Inc which was a novel technique especially for uropathogenic strains. These plates were unique in helping us identify different phenotypes within a limited time period. The results of our tests showed, some isolates whose phenotypic behavior differed, in terms of growth and motility when compared to the wild type. Antibiotic susceptibility tests enhanced our understanding of determinants of resistance which was crucial for the management of urinary tract infections. Phenotype micro array profiles generated for 2 strains, the wild type E. coli strain and one uropathogenic strain showed a total of 37 differences in carbon, nitrogen, sulfur and phosphorus metabolism in these strains. These differences indicated the likely genome variations between two strains, a fact that was shown for recently sequenced UTI strain CFT073. Therefore phenotypic characterization was useful in establishing genetic variability of the isolates and more specifically of E. coli strains causing urinary tract infections.

Book Characterization of Eight Temperature Sensitive Mutants of Escherichia Coli by Genetic Analysis and Molecular Cloning

Download or read book Characterization of Eight Temperature Sensitive Mutants of Escherichia Coli by Genetic Analysis and Molecular Cloning written by Katherine Armstrong and published by . This book was released on 1981 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Characterization of Escherichia Coli K 12 Mutants with Increased Expression of an Osmotically Regulated Gene

Download or read book Characterization of Escherichia Coli K 12 Mutants with Increased Expression of an Osmotically Regulated Gene written by Elizabeth Anne DiBlasio and published by . This book was released on 1986 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Journal of Bacteriology

Download or read book Journal of Bacteriology written by Charles-Edward Amory Winslow and published by . This book was released on 1984 with total page 1044 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book American Doctoral Dissertations

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1982 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Cumulated Index Medicus

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Dissertation Abstracts International

Download or read book Dissertation Abstracts International written by and published by . This book was released on 1998 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Nuclear Science Abstracts

Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1975-06 with total page 1162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Comprehensive Virology

    Book Details:
  • Author : Heinz Fraenkel-Conrat
  • Publisher : Springer Science & Business Media
  • Release : 2012-12-06
  • ISBN : 1461566932
  • Pages : 473 pages

Download or read book Comprehensive Virology written by Heinz Fraenkel-Conrat and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: The time seems ripe for a critical compendium of that segment of the biological universe we call viruses. Virology, as a science, having passed only recently through its descriptive phase of naming and num bering, has probably reached that stage at which relatively few new truly new-viruses will be discovered. Triggered by the intellectual probes and techniques of molecular biology, genetics, biochemical cytology, and high resolution microscopy and spectroscopy, the field has experienced a genuine information explosion. Few serious attempts have been made to chronicle these events. This comprehensive series, which will comprise some 6000 pages in a total of about 18 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. In this way, we hope to place the entire field in perspective, and to develop an invalua ble reference and sourcebook for researchers and students at all levels. This series is designed as a continuum that can be entered anywhere, but which also provides a logical progression of developing facts and integrated concepts.

Book Comprehensive Dissertation Index

Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1989 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Molecular Basis of Bacterial Pathogenesis

Download or read book Molecular Basis of Bacterial Pathogenesis written by Barbara H. Iglewski and published by Elsevier. This book was released on 1990-01-01 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Basis of Bacterial Pathogenesis focuses on the molecular mechanism of disease associated with bacterial pathogens. Topics covered include the population genetics of bacterial pathogenesis; environmental modulation of gene expression in gram-negative pathogens; and bacterial invasion and intracellular growth. Bacterial toxins are also discussed. This volume is comprised of 20 chapters and begins with an overview of pathogenesis, paying particular attention to common elements and genetic mechanisms of regulation. The discovery that many bacterial pathogens are clonal, with individual clones often having a greater virulence than others, is then considered. The next section deals with the regulation of synthesis of surface components and their role in colonization of the host and/or evasion of the host immune defense systems; antigenic variation and its role in evasion of the host immune response; and the role of iron acquisition systems in the colonization of the host. Subsequent chapters explore the invasion and intracellular growth of facultative and obligate intracellular parasites. The last section is devoted to studies on the role of bacterial toxic products in pathogenesis. Bacterial lipopolysaccharides (endotoxins) and exotoxins are described. This book should be of interest to molecular biologists, physiologists, clinical specialists, pathologists, and geneticists.