Download or read book Plant Gene Transfer and Expression Protocols written by Heddwyn Jones and published by Humana. This book was released on 2013-08-21 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio technological products of industrial importance.
Download or read book Genetic Engineering of Plants written by National Research Council and published by National Academies Press. This book was released on 1984-02-01 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."
Download or read book Gene Transfer to Plants written by Ingo Potrykus and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Download or read book Alien Gene Transfer in Crop Plants Volume 1 written by Aditya Pratap and published by Springer Science & Business Media. This book was released on 2013-11-01 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The 1st volume entitled, ‘Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment” will deal exclusively with the process and methodology. The contents of this volume have been designed to appraise the readers with all the theoretical and practical aspects of wide hybridization and gene transfer like processes and methods of gene transfer, role of biotechnology with special reference to embryo rescue, genetic transformation, protoplast fusion and molecular marker technology, problems such as cross incompatibility and barriers to distant hybridization and solutions to overcome them. Since wild and weedy relatives of crop plants may have negative traits associated with them, there are always possibilities of linkage drag while transferring alien alleles. Therefore, problems and limitations of alien gene transfer from these species will also be discussed in this series. Further, the associated risks with this and assessment of risks will also be given due weightage.
Download or read book Genetic Transformation of Plants written by John Flex Jackson and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.
Download or read book Plant Genetic Transformation Technology written by Wagdy Sawahel and published by Daya Books. This book was released on 1997 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology. Contents Chapter 1: Genetic Transformation; History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability; Chapter 2: Gene Delivery systems; Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication; Chapter 3: Strategies for Improving Transformation Efficiency; Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence; Chapter 4: Organelle Transformation; Chapter 5: Shotgun Transformation; Plasmid rescue, Gene rescue, Promoter & enhancer rescue.
Download or read book Alien Gene Transfer in Crop Plants Volume 2 written by Aditya Pratap and published by Springer. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The second volume entitled, “Alien Gene Transfer in Crop Plants: Achievements and Impact” will deal more with the practical aspects. This volume will cover achievements of alien gene transfer in major food crops of the world and their impact on development of newer genetic variability and additional avenues for selection; development of superior cultivars for increased yield, resistance to biotic and abiotic stresses, improved nutritional and industrial quality; innovation of new techniques and positive as well as negative environmental implications. This volume has been divided into four groups with an aim to cover all major cereals, pulses, oilseeds and other crops (vegetable and horticultural crops) which are of economic importance.
Download or read book Transgenic Organisms and Biosafety written by Erwin R. Schmidt and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene/biotechnology and the broad use of genetically modified organisms is rapidly developing into a commercial enterprise. In spite of the pace of scientific progress in this field, a partly emotional debate is still being carried on by the general public and on the part of the consumer about the potential risks linked to this new technology. Especially when genetically modified organisms (GMOs) are tested in the open field, the question of "safety" is controversially discussed among different groups in society. Environmentalists are worried about unforeseeable dangers inherent in the release of a living organism, assuming that special risks are linked to organisms which have been modified by molecular biologists using tools developed in the laboratory. Frequently quoted potential hazards are the phenomena of "gene escape" and "genetic pollution", meaning the very interesting question of natural (horizontal) gene transfer. This phenomenon of horizontal gene transfer is in fact well known to scientists studying prokaryotes. Many prokaryotes are able to exchange DNA readily between individuals of different species or are even able to take up DNA from the extracellular environment by the process of genetic transformation, which has already been known for more than 65 years. For eukaryotes, however, the evidence of natural horizontal gene transfer is very limited.
Download or read book Transgenic Crop Plants written by Chittaranjan Kole and published by Springer Science & Business Media. This book was released on 2010-01-13 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.
Download or read book Transgenic Plants and Crops written by George C. Khachatourians and published by CRC Press. This book was released on 2002-03-26 with total page 910 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions from nearly 130 internationally renowned experts in the field, this reference details advances in transgenic plant construction and explores the social, political, and legal aspects of genetic plant manipulation. It provides analyzes of the history, genetics, physiology, and cultivation of over 30 species of transgenic seeds, fruits, and vegetables. Stressing the impact of genetic engineering strategies on the nutritional and functional benefit of foods as well as on consumer health and the global market economy, the book covers methods of gene marking, transferring, and tagging public perceptions to the selective breeding, hybridization, and recombinant DNA manipulation of food.
Download or read book Agrobacterium From Biology to Biotechnology written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-12-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Download or read book Alien Gene Transfer in Crop Plants Volume 2 written by Aditya Pratap and published by Springer Science & Business Media. This book was released on 2014-02-03 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The second volume entitled, “Alien Gene Transfer in Crop Plants: Achievements and Impact” will deal more with the practical aspects. This volume will cover achievements of alien gene transfer in major food crops of the world and their impact on development of newer genetic variability and additional avenues for selection; development of superior cultivars for increased yield, resistance to biotic and abiotic stresses, improved nutritional and industrial quality; innovation of new techniques and positive as well as negative environmental implications. This volume has been divided into four groups with an aim to cover all major cereals, pulses, oilseeds and other crops (vegetable and horticultural crops) which are of economic importance.
Download or read book Biolistic DNA Delivery in Plants written by Sachin Rustgi and published by Humana. This book was released on 2021-04-25 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.
Download or read book Transgenic Crops of the World written by Ian S. Curtis and published by Springer Science & Business Media. This book was released on 2012-08-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.
Download or read book Horizontal Gene Transfer written by Michael Syvanen and published by Academic Press. This book was released on 2001-12-19 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Horizontal Gene Transfer has been organized to provide a concise and up-to-date coverage of the most important discoveries in this fascinating field. Written by the most prominent gene transfer and genome analytical scientists, this book details experimental evidence for the phenomenon of horizontal gene transfer and discusses further evidence provided by the recent completion of genomic sequences from Archea, Bacteria, and Eucarya members. The relevance of horizontal gene transfer to plant and metazoan taxonomy, GM foods, antibiotic resistance, paleontology, and phylogenetic reconstruction is also explored. Horizontal Gene Transfer is essential for microbiologists, geneticists, biochemists, evolutionary biologists, infectious disease specialists, paleontologists, ecologists, and researchers working in plant/animal systematics and agriculture with an interest in gene transfer. This includes scientific researchers from government and industry concerned with the release of genetically modified organisms. Up-to-the-minute reviews, maps, conclusions, urls to relevant websites and colour figures Unique chapters, for example one written by paleontologists presents data for horizontal gene transfer from fingerprints form the fossil record
Download or read book Gene Transfer written by R. Kucherlapati and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic analysis of microbial systems provided us with the foundation for un derstanding gene structure, expression, and regulation. It was long felt that the ability to generate mutants and conduct genetic studies in mammalian systems would prove to be equally useful. However, genetic analysis based on sexual systems is difficult in mammals because of the long generation times and the inability to perform controlled matings. As a result, genetic analysis of mam malian systems had to await the development of parasexual systems. This book is an attempt to bring together descriptions of a number of these parasexual systems. A common theme of all the parasexual systems is the transfer of genetic information from a defined source into a specific cell type. This volume deals with a number of methods of gene transfer into mammalian cells. The early methods of gene transfer involved transfer of relatively large amounts of genetic information. These include somatic cell hybridization, microcell fusion, and chromosome transfer, which constitute the first part of this book. Each of these methods has already proven to be of enormous value in arriving at a genetic understanding of the mammalian genome. Development of recombinant DNA methods, and the ability to introduce purified DNA into mammalian cells, has had a significant impact on our ability to dissect important aspects of mammalian gene expression and regulation. The second part of this book deals with gene transfer systems involving defined nucleic acid sequences.
