Download or read book Gene Transfer in the Cardiovascular System written by Keith L. March and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "Artificial Self-Assembling Systems for Gene Transfer", brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: • size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . • potential tumorigenesis (e. g. retrovirus) • potential for insertional mutagenesis (greater than plasmid based systems) • potential imrnunogenicity (e. g.

Download or read book Cardiac Cell and Gene Transfer written by Joseph M. Metzger and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heart disease is the leading cause of death in developed countries. Recent experimental advances featuring cellular, molecular, and genetic tools and technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications is designed to provide the reader with up-to-date coverage of a myriad of specific methodo- gies and protocols for gene and cell transfer to the myocardium. Each chapter features a “Notes” section that provides useful “how to” problem-solving insights that are often left unstated in standard published protocols. Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification. Detailed step-by-step methods and applications for first/second-generation adenoviral vectors, adeno-associated vectors, gutted adenoviral vectors, and lentiviral vectors are included. Additionally, detailed methods for cardiac cell grafting and transplantation are provided, and these chapters highlight the prospects of cell-based therapies for cardiac repair. The book also covers specific in vivo techniques for cardiac gene transfer, and specifies subsequent cellular and organ-level physiological assessment techniques and protocols. Accordingly, this book is designed for basic science and clinical researchers in the academic, pharmaceutical, and biotechnology sectors of the cardiovascular community.

Download or read book Regulation of Tissue Oxygenation Second Edition written by Roland N. Pittman and published by Biota Publishing. This book was released on 2016-08-18 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: This presentation describes various aspects of the regulation of tissue oxygenation, including the roles of the circulatory system, respiratory system, and blood, the carrier of oxygen within these components of the cardiorespiratory system. The respiratory system takes oxygen from the atmosphere and transports it by diffusion from the air in the alveoli to the blood flowing through the pulmonary capillaries. The cardiovascular system then moves the oxygenated blood from the heart to the microcirculation of the various organs by convection, where oxygen is released from hemoglobin in the red blood cells and moves to the parenchymal cells of each tissue by diffusion. Oxygen that has diffused into cells is then utilized in the mitochondria to produce adenosine triphosphate (ATP), the energy currency of all cells. The mitochondria are able to produce ATP until the oxygen tension or PO2 on the cell surface falls to a critical level of about 4–5 mm Hg. Thus, in order to meet the energetic needs of cells, it is important to maintain a continuous supply of oxygen to the mitochondria at or above the critical PO2 . In order to accomplish this desired outcome, the cardiorespiratory system, including the blood, must be capable of regulation to ensure survival of all tissues under a wide range of circumstances. The purpose of this presentation is to provide basic information about the operation and regulation of the cardiovascular and respiratory systems, as well as the properties of the blood and parenchymal cells, so that a fundamental understanding of the regulation of tissue oxygenation is achieved.

Download or read book Cardiovascular Genomics written by Mohan K. Raizada and published by Springer Science & Business Media. This book was released on 2008-02-24 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recognized scientists and clinicians from around the world discuss the most recent molecular approaches to understanding the cardiovascular system in both health and disease. The authors focus on all components of the system, including blood vessels, heart, kidneys, and the brain, and cover disease states ranging from vascular and cardiac dysfunction to stroke and hypertension. The methods described for identifying the genes that cause susceptibility to cardiovascular diseases emphasize the possibility of discovering new drug targets. Authoritative and ground-breaking, Cardiovascular Genomics offers an unprecedented examination of both the cutting-edge scientific approaches now possible and the results obtained from them in the new science of cardiovascular genomics.

Download or read book Cardiac Gene Therapy written by Kiyotake Ishikawa and published by Springer Nature. This book was released on 2022-08-30 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter “Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Molecular Genetics Gene Therapy of Cardiovascular Diseases written by S. C. Mockrin and published by CRC Press. This book was released on 1996-01-02 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides detailed discussions of the most recent developments in gene mapping and manipulation to improve the diagnosis, detection, prevention, and treatment of various cardiovascular diseases--including hypertension, arrhythmias, inherited cariomyopathies, hyperlipidemia, aortic aneurysms, Marfan syndrome, and myocardial infarction. Written by nea

Download or read book Cardiovascular Genetics and Genomics for the Cardiologist written by Victor J. Dzau, MD and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until recently, a modest knowledge of genetics was more than adequate for the daily practice of clinical cardiology, but advances in genetics and genomics are changing cardiovascular medicine in fundamental ways. The identification of the genetic basis of several forms of dyslipidemia, hypertension, diabetes, cardiomyopathies, and vascular diseases signalled the new importance of genetics in clinical medicine. In this timely volume, Drs. Dzau and Liew – both pioneers in the area – help cardiologists understand: • how cardiovascular genetics may remodel the way cardiovascular medicine is practiced • what material has immediate relevance to the practicing clinician • how to incorporate genetics and genomics in your practice to ensure up-to-date patient care The book opens with introductory chapters, then discusses: • cardiovascular single gene disorders • cardiovascular polygenic disorders • therapies and applications Outstanding contributors write on their areas of expertise, making Cardiovascular Genetics and Genomics for the Cardiologist both authoritative and comprehensive. If you want to gain a better appreciation of how genetics and genomics are already shaping current practice and may potentially revolutionize cardiology, look no further than this dependable reference.

Download or read book Post Genomic Cardiology written by José Marín-García and published by Academic Press. This book was released on 2014-05-09 with total page 935 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this second edition of Post-Genomic Cardiology, developing and new technologies such as translational genomics, next generation sequencing (NGS), bioinformatics, and systems biology in molecular cardiology are assessed in light of their therapeutic potential. As new methods of mutation screening emerge, both for the genome and for the “epigenome, comprehensive understanding of the many mutations that underlie cardiovascular diseases and adverse drug reactions is within our reach. This book, written by respected cardiologist José Marín-García, features discussion on the Hap-Map: the largest international effort to date aiming to define the differences between our individual genomes. This unique reference further reviews and investigates genome sequences from our evolutionary relatives that could help us decipher the signals of genes, and offers a comprehensive and critical evaluation of regulatory elements from the complicated network of the background DNA. Offers updated discussion of cutting-edge molecular techniques including new genomic sequencing / NGS / Hap-Map / bioinformatics / systems biology approaches Analyzes mitochondria dynamics and their role in cardiac dysfunction, up-to-date analysis of cardio-protection, and cardio-metabolic syndrome Presents recent translational studies, gene therapy, transplantation of stem cells, and pharmacological treatments in CVDs

Download or read book Vascular Disease written by Andrew H. Baker and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology has revolutionized research into vascular disease. Over the past 20 years molecular techniques have enabled us to both elucidate - lecular mechanisms in vascular disease and identify appropriate therapies. The vast explosion in technical knowledge and the array of protocols that become more advanced and intricate by the day lead us into new and exciting areas of research that were previously unobtainable. Vascular Disease: Molecular Biology and Gene Transfer Protocols - scribes today’s most powerful molecular methods for the investigation of the pathogenesis of vascular disease. The protocols are highly detailed, allowing beginners who have little experience in either vascular biology or molecular biology to embark on new molecular projects. This book is also suited to more experienced molecular biologists who wish to grasp new methods for stu- ing the involvement of genes in normal vascular physiology and in diseased states. It is well established that cardiovascular disease progression has a s- stantial genetic influence. Part I describes three methods that have been used successfully to identify specific mutations in candidate genes involved in c- diovascular disorders. These mutations include both single-stranded conf- mational polymorphism analysis and heteroduplex detection methods. In addition, technology to map new genes to specific regions of chromosomes by high-resolution mapping is described.

Download or read book Cardiac Gene Therapy written by Kiyotake Ishikawa and published by Humana. This book was released on 2016-12-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.

Download or read book Genes and Cardiovascular Function written by Bohuslav Ostadal and published by Springer Science & Business Media. This book was released on 2011-08-09 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular medicine have led to pronounced new developments in experimental and clinical cardiology. In the embrace of modern molecular biology and bridging the gap between the clinical and the genomic, cardiovascular medicine has seen major strides in the understanding of the molecular mechanisms that drive disease progression. The ability to rapidly identify candidate human genes for cardiovascular diseases lends itself to the development of diverse strategies for disease treatment and management. The wide variety of gene expressions proffers excellent targets for novel therapeutics. Gene therapy is steadily increasing in viability and represents a fascinating arena of research and clinical focus. This book is based on two international Mendel symposia on “Genes and the Heart,” joint meetings of the Japanese and European sections of the International Academy of Cardiovascular Sciences. Highlighting selected symposia contributions, this book explores the role of molecular biology and genetics in the basic knowledge, genesis, and clinical interventions of cardiovascular diseases.

Download or read book Cardioselective Nitric Oxide Synthase Gene Transfer to Target Myocardial Ischemia written by Zsolt Szelid and published by Leuven University Press. This book was released on 2005 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a Ph.D. dissertation. Introduction: Cardiovascular and myocardial gene transfer, Gene delivery strategies to the cardiovascular system, Gene vector design, Adenovirus-mediated immunity and cardiovascular gene transfer, Myocardial gene transfer to target myocardial ischemia - reperfusion injury; Specific aims; Materials and methods: Construction of recombinant virus, Myocardial transfer and anti-adenoviral immunity, Gene transfer and myocardial ischemia-reperfusion injury, Statistical analysis; Results: Anti-adenoviral immunity and myocardial adenoviral gene transfer, Gene transfer and myocardial ischemia-reperfusion injury; Discussion: Pre-existing anti-adenoviral immunity and adenovirus-mediated myocardial gene transfer, Intramyocardial NOS3 gene transfer and adenovirus-mediated immune responses, Cardioselective NOS3 gene transfer and myocardial protection from reperfusion injury; General conclusions.

Download or read book Oversight and Review of Clinical Gene Transfer Protocols written by Institute of Medicine and published by National Academies Press. This book was released on 2014-03-27 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene transfer research is a rapidly advancing field that involves the introduction of a genetic sequence into a human subject for research or diagnostic purposes. Clinical gene transfer trials are subject to regulation by the U.S. Food and Drug Administration (FDA) at the federal level and to oversight by institutional review boards (IRBs) and institutional biosafety committees (IBCs) at the local level before human subjects can be enrolled. In addition, at present all researchers and institutions funded by the National Institutes of Health (NIH) are required by NIH guidelines to submit human gene transfer protocols for advisory review by the NIH Recombinant DNA Advisory Committee (RAC). Some protocols are then selected for individual review and public discussion. Oversight and Review of Clinical Gene Transfer Protocols provides an assessment of the state of existing gene transfer science and the current regulatory and policy context under which research is investigated. This report assesses whether the current oversight of individual gene transfer protocols by the RAC continues to be necessary and offers recommendations concerning the criteria the NIH should employ to determine whether individual protocols should receive public review. The focus of this report is on the standards the RAC and NIH should use in exercising its oversight function. Oversight and Review of Clinical Gene Transfer Protocols will assist not only the RAC, but also research institutions and the general public with respect to utilizing and improving existing oversight processes.

Download or read book Applications of Biotechnology in Cardiovascular Therapeutics written by Kewal K. Jain and published by Springer Science & Business Media. This book was released on 2011-07-25 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: For physicians, surgeons, and scientists working on cardiovascular disorders, Applications of Biotechnology in Cardiovascular Therapeutics serves as an invaluable reference by collecting the essential writings of Dr. Kewal K. Jain on the topics of biotechnology as they relate to cardiovascular disease. This thorough volume includes such subjects as biotechnology and therapeutic delivery to the cardiovascular system, cell-selective targeted drug delivery, cell and gene therapies, including antisense and RNA interference, cutting-edge gene therapy approaches, as well as personalized cardiology as a way of integrating new technologies into the selection of the best possible treatment for an individual patient. Selected references from recent literature are collected for each chapter, and the text is supplemented by a variety of useful tables and figures. Comprehensive and up-to-date, Applications of Biotechnology in Cardiovascular Therapeutics will be tremendously useful for those working in life sciences and the pharmaceutical sciences, and the inclusion of some basics of cardiovascular diseases will greatly benefit nonmedical readers as well.

Download or read book Immunopharmacology written by Manzoor M. Khan and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decades, with the introduction of the recombinant DNA, hybridoma and transgenic technologies there has been an exponential evolution in understanding the pathogenesis, diagnosis and treatment of a large number of human diseases. The technologies are evident with the development of cytokines and monoclonal antibodies as therapeutic agents and the techniques used in gene therapy. Immunopharmacology is that area of biomedical sciences where immunology, pharmacology and pathology overlap. It concerns the pharmacological approach to the immune response in physiological as well as pathological events. This goals and objectives of this textbook are to emphasize the developments in immunology and pharmacology as they relate to the modulation of immune response. The information includes the pharmacology of cytokines, monoclonal antibodies, mechanism of action of immune-suppressive agents and their relevance in tissue transplantation, therapeutic strategies for the treatment of AIDS and the techniques employed in gene therapy. The book is intended for health care professional students and graduate students in pharmacology and immunology.

Download or read book Adenoviral Vectors for Gene Therapy written by David T. Curiel and published by Academic Press. This book was released on 2016-03-10 with total page 868 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adenoviral Vectors for Gene Therapy, Second Edition provides detailed, comprehensive coverage of the gene delivery vehicles that are based on the adenovirus that is emerging as an important tool in gene therapy. These exciting new therapeutic agents have great potential for the treatment of disease, making gene therapy a fast-growing field for research. This book presents topics ranging from the basic biology of adenoviruses, through the construction and purification of adenoviral vectors, cutting-edge vectorology, and the use of adenoviral vectors in preclinical animal models, with final consideration of the regulatory issues surrounding human clinical gene therapy trials. This broad scope of information provides a solid overview of the field, allowing the reader to gain a complete understanding of the development and use of adenoviral vectors. Provides complete coverage of the basic biology of adenoviruses, as well as their construction, propagation, and purification of adenoviral vectors Introduces common strategies for the development of adenoviral vectors, along with cutting-edge methods for their improvement Demonstrates noninvasive imaging of adenovirus-mediated gene transfer Discusses utility of adenoviral vectors in animal disease models Considers Federal Drug Administration regulations for human clinical trials

Download or read book Vascular Biology of the Placenta written by Yuping Wang and published by Biota Publishing. This book was released on 2017-06-23 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: The placenta is an organ that connects the developing fetus to the uterine wall, thereby allowing nutrient uptake, waste elimination, and gas exchange via the mother's blood supply. Proper vascular development in the placenta is fundamental to ensuring a healthy fetus and successful pregnancy. This book provides an up-to-date summary and synthesis of knowledge regarding placental vascular biology and discusses the relevance of this vascular bed to the functions of the human placenta.