Download or read book Gene Therapy for HIV written by Gerhard Bauer and published by Springer Science & Business Media. This book was released on 2014-02-07 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Brief describes the concept and realization of gene therapy for HIV from the unique historic perspective and insight of two pioneers of the clinical applications of stem cell gene therapy for HIV. Gerhard Bauer applied ribozyme-anti-HIV and other vectors to manufacture clinical grade, HIV-resistant hematopoietic stem cells for the first patients that received stem cell gene therapy for HIV, including the first child in the world and the first fully marrow-ablated HIV infected patient. Joseph Anderson developed the most recent and most potent combination anti-HIV lentiviral vectors and pluripotent stem cell applications for HIV gene therapy and tested these in the appropriate in vitro and vivo models, paving the way for novel HIV gene therapy approaches to possibly cure patients. In Gene Therapy for HIV, Bauer and Anderson discuss the unique aspects of this therapy, including its limitations and proper safety precautions and outline a path for a possible functional cure for HIV using stem cell gene therapy based on a cure already achieved with a bone marrow stem cell transplantation performed in Germany using donor stem cells with a naturally arising CCR5 mutation. In addition, the Brief provides a thorough and methodical explanation of the basics of gene therapy, gene therapy vector development, in vitro and in vivo models for HIV gene therapy and clinical applications of HIV gene therapy, including Good Manufacturing Practices.

Download or read book Gene Therapy for HIV Infection written by Clay Smith and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: ince the early 1980s, the HIV epidemic has been raging within the S 1 United States and around the world. Drug therapy for HIV infection has not been curative, prompting the search for alternative strategies to control HIV infection within infected persons. One potential alterna tive to drug therapy is a developing medical technology termed gene therapy. 2 Gene therapy involves introducing genetic elements into popu lations of cells in order to correct or prevent a pathologic process. A large number of gene therapy strategies have been developed in an at tempt to inhibit HIV expression and spread. These strategies fall into two general categories, genetic modification of cells in order to elicit an immune response against HIV and genetic modification of the target cells of HIV infection in order to block HIV expression and reproduction. In the first strategy, termed genetic immunotherapy by some, genetic material encoding HIV proteins is introduced into patient's cells in order to stimulate a cellular immune response above and beyond 3 5 that stimulated by the viral infection itself. - Two general genetic im munotherapy strategies have been developed. Genes encoding HIV pro teins have been directly injected into the dermis or muscle tissue of patients. These genes have been encoded in plasmids or viral DNA and have been injected either in the form of naked DNA or complexed with lipids.

Download or read book Gene Therapy for HIV and Chronic Infections written by Ben Berkhout and published by Springer. This book was released on 2015-03-10 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book centers on gene therapy and gene transfer approaches to prevent or treat chronic virus infections. The main focus is on the Big Three: human immunodeficiency virus (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV). Ample anti-HIV drugs are currently available in the clinic and the development of an effective combination therapy has dramatically improved the lifespan and quality of life of infected individuals. A similar trend can already be recognized for HBV and HCV: the development of multiple (directly acting) antiviral drugs and plans to control or even cure the infection. However, approaches that help prevent infection, or which provide long-lasting treatment (such as a cure) remain important goals. Immunization through gene transfer vehicles encoding immunogenic viral proteins shows promise in preventing infections with complex, highly variable, viruses such as HIV-1 or HCV. Gene therapy applications for virus infections have been discussed since the early 1990’s. Whereas a true cure seems difficult to achieve for HIV-1 due to its intrinsic property to deposit its genome into that of the host, such attempts may be within reach for HCV where spontaneous viral clearance occurs in a small percentage of the infected individuals. The prospect of original gene therapy approaches may provide alternative ways to reach the same endpoint by, for example, silencing of CCR5 expression post-transcriptionally. Many alternative antiviral strategies have been developed based on a variety of novel molecular methods: e.g. ribozymes. Some studies have progressed towards pre-clinical animal models and a few antiviral gene therapies have progressed towards clinical trials. This book provides an overview of this rapidly progressing field, while focusing on the interface of gene therapy and immunology/vaccinology.

Download or read book Immunotreatment and Gene Therapy of HIV Infection written by Ulrich R Hengge and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Gene Therapy for HIV Infection written by Clay Smith and published by Springer. This book was released on 2014-01-15 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses primarily on the development of effective gene therapy approaches for HIV infection, including the improvement of gene transfer vectors, procedures for the ex-vivo manipulation and transduction of T-lymphocytes and hematopoietic stem cells, and preclinical testing of HIV inhibition strategies. It provides a balanced perspective of the many accomplishments in this field as well as the problems which need to be solved in order to develop safe and effective therapies which benefit persons with HIV infection.

Download or read book Gene Therapy for Viral Infections written by Patrick Arbuthnot and published by Academic Press. This book was released on 2015-06-01 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene Therapy for Viral Infections provides a comprehensive review of the broader field of nucleic acid and its use in treating viral infections. The text bridges the gap between basic science and important clinical applications of the technology, providing a systematic, integrated review of the advances in nucleic acid-based antiviral drugs and the potential advantages of new technologies over current treatment options. Coverage begins with the fundamentals, exploring varying topics, including harnessing RNAi to silence viral gene expression, antiviral gene editing, viral gene therapy vectors, and non-viral vectors. Subsequent sections include detailed coverage of the developing use of gene therapy for the treatment of specific infections, the principles of rational design of antivirals, and the hurdles that currently face the further advancement of gene therapy technology. - Provides coverage of gene therapy for a variety of infections, including HBV, HCV, HIV, hemorrhagic fever viruses, and respiratory and other viral infections - Bridges the gap between the basic science and the important medical applications of this technology - Features a broad approach to the topic, including an essential overview and the applications of gene therapy, synthetic RNA, and other antiviral strategies that involve nucleic acid engineering - Presents perspectives on the future use of nucleic acids as a novel class of antiviral drugs - Arms the reader with the cutting-edge information needed to stay abreast of this developing field

Download or read book Immunotreatment and Gene Therapy of HIV Infection written by Ulrich R. Hengge and published by . This book was released on 2004 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tremendous progress in the understanding of the immune pathogenesis of HIV disease has been made in the last two decades like in no other field of science ever before. Besides the inhibition of viral replication with highly active antiretroviral therapy (HAART) immunoreconstitution remains an important mainstay to be achieved in the battle against HIV. Intensive research has led to worldwide phase-III studies of immunotreatment of HIV infection such as the SILCAAT and ESPRIT trials to boost existing immune responses and to restore the immune system leading to the control of viral replication. The science and practical management of immunotherapy of HIV patients is described by famous experts from immunology, virology, gene therapy, dermatology, and clinical medicine.

Download or read book Development and Applications of Vaccines and Gene Therapy in AIDS written by Gaetano Giraldo and published by S. Karger AG (Switzerland). This book was released on 1996-01-01 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication is an overview of the exciting discoveries in the field of AIDS which were discussed at the workshop 'Development and Applications of Vaccines and Gene Therapy in AIDS' (Naples, June 1995). Various articles present the latest advances in the development of vaccines, focusing on the analysis of immunogenetic epitopes and models of expression and/or administration. Some contributions describe the results of recent vaccine trials with animal models while others concentrate on human clinical trials. Furthermore, articles on new approaches in the field of gene therapy are included. The data compiled in this outstanding volume is essential not only for biomedical scientists but also for all researchers interested in the latest developments in the field of HIV/AIDS.

Download or read book Immunopharmacology written by Manzoor M. Khan and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decades, with the introduction of the recombinant DNA, hybridoma and transgenic technologies there has been an exponential evolution in understanding the pathogenesis, diagnosis and treatment of a large number of human diseases. The technologies are evident with the development of cytokines and monoclonal antibodies as therapeutic agents and the techniques used in gene therapy. Immunopharmacology is that area of biomedical sciences where immunology, pharmacology and pathology overlap. It concerns the pharmacological approach to the immune response in physiological as well as pathological events. This goals and objectives of this textbook are to emphasize the developments in immunology and pharmacology as they relate to the modulation of immune response. The information includes the pharmacology of cytokines, monoclonal antibodies, mechanism of action of immune-suppressive agents and their relevance in tissue transplantation, therapeutic strategies for the treatment of AIDS and the techniques employed in gene therapy. The book is intended for health care professional students and graduate students in pharmacology and immunology.

Download or read book HIV written by Frederic Bushman and published by Cold Spring Harbor Perspective. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basic biology of the HIV virus provides a model for a more general understanding of retroviruses, and the worldwide epidemic of AIDS makes research into the disease process and potential therapies among the most critical in biomedical science. This book explores work on the molecular biology of HIV, host-virus interactions, host immune responses, HIV transmission, and more.

Download or read book Denying AIDS written by Seth C. Kalichman and published by Springer Science & Business Media. This book was released on 2009-01-16 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Paralleling the discovery of HIV and the rise of the AIDS pandemic, a flock of naysayers has dedicated itself to replacing genuine knowledge with destructive misinformation—and spreading from the fringe to the mainstream media and the think tank. Now from the editor of the journal AIDS and Behavior comes a bold exposé of the scientific and sociopolitical forces involved in this toxic evasion. Denying AIDS traces the origins of AIDS dissidents disclaimers during the earliest days of the epidemic and delves into the psychology and politics of the current denial movement in its various incarnations. Seth Kalichman focuses not on the “difficult” or doubting patient, but on organized, widespread forms of denial (including the idea that HIV itself is a myth and HIV treatments are poison) and the junk science, faulty logic, conspiracy theories, and larger forces of homophobia and racism that fuel them. The malignant results of AIDS denial can be seen in those individuals who refuse to be tested, ignore their diagnoses, or reject the treatments that could save their lives. Instead of ignoring these currents, asserts Kalichman, science has a duty to counter them. Among the topics covered: Why AIDS denialism endures, and why science must understand it. Pioneer virus HIV researcher Peter Duesberg’s role in AIDS denialism. Flawed immunological, virological, and pharmacological pseudoscience studies that are central to texts of denialism. The social conservative agenda and the politics of AIDS denial, from the courts to the White House. The impact of HIV misinformation on public health in South Africa. Fighting fiction with reality: anti-denialism and the scientific community. For anyone affected by, interested in, or working with researchers in HIV/AIDS, and public health professionals in general, the insight and vision of Denying AIDS will inspire outrage, discussion, and ultimately action. See http://denyingaids.blogspot.com/ for more information.

Download or read book Gene Therapy and Its Potential as a Treatment for HIV written by Deirdre McDermott and published by . This book was released on 1994 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Efficacy Safety and Delivery of Anti HIV Short hairpin RNA Molecules for Use in HIV Gene Therapy written by Camille Malard and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Although combination antiretroviral therapy can prevent the progression of human immunodeficiency virus type 1 (HIV) infection to acquired immunodeficiency syndrome, it cannot cure the infection, due in part to the persistence of latent viral reservoirs. Alternatively, gene therapy could be used to develop an HIV cure: a patient’s hematopoietic stem cells (HSCs) could be modified ex vivo to express molecules that inhibit HIV replication and transferred back to the patient where they could serve as a source of HIV-resistant immune cells. Many molecules have been developed to inhibit HIV replication, and one major class are the short hairpin (sh)RNAs which can direct the host RNA interference machinery against HIV RNAs. Although many anti-HIV shRNAs have been developed and some have been used in gene therapy clinical trials, little is known on the comparative efficacy and toxicity of molecules designed by different groups. Also, while HIV-based lentiviral vectors (LVs) are the leading gene delivery tool in HIV gene therapy, clinical trials have been hampered by low HSC transduction, in part because anti-HIV molecules can inhibit their production.Our first hypothesis was that the delivery of anti-HIV shRNAs can be enhanced by optimizing LV production. Our second hypothesis was that we can identify optimal anti-HIV shRNA candidates for gene therapy by comparing the efficacy, toxicity, and target site conservation of both novel and previously-designed shRNAs.Our first aim was to enhance the LV-mediated delivery of anti-HIV shRNAs. We identified a combination of HIV-based LV production plasmids that generated higher titers of LVs compared to a combination recently used in a gene therapy clinical trial. The production of these LVs was inhibited by anti-HIV shRNAs targeting the gag or tat/rev genes, but this inhibition was overcome by increasing the concentration of the packaging plasmid during LV production, as this plasmid encodes the components targeted by our molecules. As an alternative strategy to overcome the inhibition of LV production by anti-HIV shRNAs, we developed a hybrid feline (F)IV:HIV LV delivery system and showed that production of these LVs was not inhibited by our anti-HIV shRNAs. However, these hybrid LVs had low transduction efficiencies in a T lymphocytic cell lines and further troubleshooting is necessary before they can be considered a viable option for gene therapy. Finally, we designed a system to evaluate the ability of gag-pol genes from different HIV strains to generate LVs, and we identified a gag-pol gene which enhances LV production compared to that of our leading packaging plasmid. Our second aim was to identify safe and potent anti-HIV shRNAs. We assessed the target site conservation of shRNAs identified by other groups, as well as that of an shRNA targeting the gag gene previously identified by our lab and an shRNA targeting the tat/rev sequence used in combination with other molecules in a gene therapy clinical trial (sh5983). We then assessed the potency of top candidates to inhibit HIV-1 production and selected the most potent molecules for further characterization in a human T lymphocytic cell line (SUP-T1). From this screen, we identified 5 highly potent shRNAs, 3 of which delayed HIV infection in SUP-T1 cells for a longer time than did sh5983. Two of these shRNAs had no noticeable cytotoxic effects and did not activate the interferon pathway in SUP-T1 cells. We have developed two methods to overcome the inhibition of LV production by anti-HIV shRNAs, and we have identified an optimal gag-pol gene sequence which increases LV production. These findings could potentially be applied in a therapeutic context to enhance the transduction of patients’ HSCs. Additionally, we have identified highly potent shRNAs which are non-cytotoxic in vitro and target highly-conserved sites in the HIV genome. As such, these shRNAs are optimal candidates for use in combination gene therapy against HIV infections"--

Download or read book Disease Control Priorities Third Edition Volume 6 written by King K. Holmes and published by World Bank Publications. This book was released on 2017-11-06 with total page 1027 pages. Available in PDF, EPUB and Kindle. Book excerpt: Infectious diseases are the leading cause of death globally, particularly among children and young adults. The spread of new pathogens and the threat of antimicrobial resistance pose particular challenges in combating these diseases. Major Infectious Diseases identifies feasible, cost-effective packages of interventions and strategies across delivery platforms to prevent and treat HIV/AIDS, other sexually transmitted infections, tuberculosis, malaria, adult febrile illness, viral hepatitis, and neglected tropical diseases. The volume emphasizes the need to effectively address emerging antimicrobial resistance, strengthen health systems, and increase access to care. The attainable goals are to reduce incidence, develop innovative approaches, and optimize existing tools in resource-constrained settings.

Download or read book Consolidated Guidelines on the Use of Antiretroviral Drugs for Treating and Preventing HIV Infection written by World Health Organization and published by . This book was released on 2016 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: These guidelines provide guidance on the diagnosis of human immunodeficiency virus (HIV) infection, the use of antiretroviral (ARV) drugs for treating and preventing HIV infection and the care of people living with HIV. They are structured along the continuum of HIV testing, prevention, treatment and care. This edition updates the 2013 consolidated guidelines on the use of antiretroviral drugs following an extensive review of evidence and consultations in mid-2015, shared at the end of 2015, and now published in full in 2016. It is being published in a changing global context for HIV and for health more broadly.

Download or read book Gene Therapy for HIV one Infection written by Ingrid Bahner and published by . This book was released on 1996 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recent Translational Research in HIV AIDS written by Yi-Wei Tang and published by IntechOpen. This book was released on 2011-11-02 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: The collective efforts of HIV/AIDS research scientists from over 16 countries in the world are included in the book. This 27-chapter Open Access book well covers HIV/AIDS translational researches on pathogenesis, diagnosis, treatment, prevention, and also those beyond conventional fields. These are by no means inclusive, but they do offer a good foundation for the development of clinical patient care. The translational model forms the basis for progressing HIV/AIDS clinical research. When linked to the care of the patients, translational researches should result in a direct benefit for HIV/AIDS patients.