Download or read book Gene Expression Analysis in Endometrial Tissue and Cultured Endometrial Cells written by Olga V. Klinkova and published by . This book was released on 2006 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Endometrial Gene Expression written by Joanne Kwak-Kim and published by Springer Nature. This book was released on 2019-11-08 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: The endometrium goes through cyclical changes under the influence of hormones, and immune effectors in the endometrium change their populations cyclically as well. Dynamic changes in immune effectors determine endometrial cytokine and chemokine milieus and, consequently, the endometrial immune response. Endometrial immune responses have been shown to be important for trophoblast invasion and early pregnancy. Indeed, dysregulated endometrial inflammatory immune responses are associated with reproductive failures, such as recurrent pregnancy losses, repeated implantation failure, and gynecological diseases with oncogenic potential. Hence, the investigation of endometrial gene expression may predict infertility, gynecological and oncological conditions. This book presents the state of the art in endometrial gene expression as well as recent developmental findings relating to reproduction and reproductive disorders. It begins with a review of the genetic regulation of urogenital tract formation and molecular mechanism underlying the physiology of menstrual cycle before moving on to the current and emerging technological advances in molecular biology. Endometrial gene expressions of immune inflammatory conditions, viral infection, metabolic and nutritional conditions, and reproductive disorders are then presented in following chapters. Examples of the use and interpretation of gene expression in clinical scenarios including recurrent pregnancy losses, infertility and multiple implantation failures are presented with currently available endometrial gene analysis. Utilizing the latest evidence and clinical guidelines, Endometrial Gene Expression will be a cutting-edge resource for gynecologist, reproductive immunologist, reproductive endocrinologists, obstetrician, maternal fetal medicine specialist and other health care providers in the field of reproductive medicine.
Download or read book Cell and Molecular Biology of Endometrial Carcinoma written by Hiroyuki Kuramoto and published by Springer Science & Business Media. This book was released on 2003-07-02 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 15th International Symposium of the Japan Human Cell Society on Cell and Molecular Biology of Endometrial Carcinoma brought together leading researchers from Japan and around the world. The papers collected here are the work of twenty-two leaders in their field and are organized in ten major categories. The first section, in vitro experimental systems, takes up the pioneering work by Kuramoto in 1968 and Nishida in 1980 in establishing, respectively, the HEC-1 and hormone-responsive endometrial carcinoma cell lines. Other topics include apoptosis, proliferation, and growth factors; cell cycle regulators; signaling pathways; angiogenesis; carcinogenesis; hormones and hormone receptors; genes and gene expression; endometrial receptivity; and chemo-resistance and -sensitivity. Presenting the latest work in the cell and molecular biology of endometrial carcinoma, this volume is a valuable resource for gynecologists, pathologists, and molecular biologists.
Download or read book The Endometrium written by Stanley R. Glasser and published by CRC Press. This book was released on 2002-05-16 with total page 1079 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Endometrium is devoted to a comprehensive multi-disciplinary account of the uterine endometrium. This book is the first to define the regulatory biological interrelationships between epithelial and stromal cell phenotypes, endothelial cells, extracellular matrix and immunobiological elements. It highlights their relevance to clinical conditions
Download or read book Xenobiotic Effects on Gene Expression in Endometrial Cells and Placental Tissue written by Grantley Dexter Charles and published by . This book was released on 1997 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Human Endometrium written by Carlo Bulletti and published by . This book was released on 1994 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structural, biochemical and clinical events related to menstruation, implantation, parturition, endometriosis, abnormal uterine bleeding and endometrial cancer are discussed in this comprehensive volume on the biological functions of the endometrium. New topics, such as the biochemical and molecular mechanisms regulating maternal embryonic interaction, are explored, and gynecologic endoscopy and therapeutic tools are discussed. The proceedings of the first conference is also available from the Academy, as volume 622 of The Annals of The New York Academy of Science.
Download or read book Functional Characterization of Human Endometrial Stem Progenitor Cells in Vitro and in Vivo written by Lina Xiang and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Functional characterization of human endometrial stem/progenitor cells in vitro and in vivo" by Lina, Xiang, 向丽娜, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Human endometrium undergoes cyclic tissue breakdown and regeneration throughout a woman of reproductive life. It has been speculated that there is a subpopulation of stem/progenitor cells residing in the endometrium responsible to its remarkable capability. One key feature that distinguishes the adult stem cells is their quiescent state within the stem cell niche. Based on this unique stem cell property, slow-cycled cells from human endometrial tissues were examined and characterized. The first aim of this study was to examine label retaining cells (LRCs) in long-term engrafted human endometrial tissues using a mouse xenotransplantation model in vivo. After various initial approaches, the maximum initial labeling of LRCs was determined and, only stromal LRCs were detected after a 12-week chase. Mesenchymal stem cell (MSCs) and stem cell-like phenotypic appearances were detected in a small proportion of stromal LRCs. The second objective was to establish a novel method for identifying slow-cycled cells from cultivated endometrial epithelial and stromal cells in vitro. The method identified cells that retained fluorescence label after long-term culture. These cells were termed fluorescence retaining cells (FRCs). The variables and the different initial approaches leading to the establishment of the protocol for the identification of FRC were optimized. The third objective further characterized the endometrial stromal FRCs. Endometrial stromal FRCs were enriched with cells having higher colony forming and self-renewal abilities when compared with non-FRCs. At subsequent passage (P2) the adult stem cell characteristics of cells derived from FRCs was more prominent and differentiated into mesenchymal lineages. Clonally derived stromal FRCs expressed higher self-renewal and pluripotent genes (BMI-1, NANGOG, OCT4, and SOX2). They also expressed endometrial mesenchymal stem cell phenotypic surface markers: CD146/PDGFRβ and W5C5, suggesting these cells might be of MSC origin. The fourth objective was to examine the role of epithelial-stromal interactions on regulation of endometrial stem cells. Menstrual cycle day 2 epithelial conditioned medium significantly enhanced the colony forming ability of endometrial stromal cells. Protein expression analysis of the early menstrual phase conditioned medium showed two cytokines: angiogenin and interleukin 8, potentially involved in the regulation of endometrial stromal stem/progenitor cells. The fifth objective was to examine the reconstitution ability of the endometrial clonogenic cells in vivo using a mouse xenotransplantation model. Endometrial clonogenic stromal cells together with epithelial cells reconstituted muscle-like tissue. Although no endometrial-like tissue was detected, the findings provide the first in vivo evidence that stromal stem/progenitor cells are present within clonogenic cells. Overall, the thesis reports the existence of slow-cycled cells from human endometrium. By using various label retention techniques in vitro and in vivo a subpopulation of slow-cycled stromal cells which exhibit characteristics properties of adult stem cells was demonstrated for the first time. DOI: 10.5353/th_b4961744 Subjects: Stem cells Endometrium
Download or read book Primary Culture Of Human Endometrial Cells As A Model To Study Tissue Released Immunoregulatory Factors written by Magdalena Staniszewska and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Problem statementImmunomodulatory factors present in microenvironment of endometrium are the important componentss regulating biological function of the uterus. Indoleamine2,3-dioxygenase enzyme (IDO) contributes to regulation of immune suppression in endometrium by tryptophan depletion and kynurenine generation. RAGE (receptor binding advanced glycation end-products) activation is another mechanism providing immunoregulatory factors such as cytokines. Understanding of the mechanisms leading to endometrial abnormalities might bring us closer to novel cancer therapeutic options for or impaired receptivity of endometrium. This requires proper models suitable for research. Here we present the patient-derived primary endometrial cells cultured as a model for studying immunoregulatory events within endometrial microenvironment.MethodsThe cells were isolated from cancerous and non-cancerous endometrial tissue during the surgical intervention. The in vitro cultured cells were cultured in the optimized medium supplemented with factors promoting growth of epithelial cancer cells. The cell proliferation rate was determined along with morphology. Immunofluorescence was used to test the expression of epithelial marker (CytK8) and MUC1 (abundant in endometrium). The presence of immune regulatory proteins, like IDO and RAGE were determined by IF and Western Blotting. In addition, kynurenine accumulation in the medium from cultured cells was analyzed by HPLC.ResultsThe human primary endometrial cells isolated from cancerous tissue showed higher propagation capability compared to the control tissue (as expressed by the number of passages carried and amount of the achieved cell number). The size and morphology were quite diverse showing a mixture of epithelial-like and fibroblastic cells. Abundant staining with CytK8 antibodies (often overexpressed on the cancer epithelial cells) was seen in all cultures confirming substantial population of the positive cells in the isolates. MUC1 protein was also seen in cultures independently of disease status. Finally, the immune regulatory proteins, i.e. IDO and RAGE were also detected by IF and WB, although at different level dependent on culture. Importantly, the cells isolated from cancerous tissue released higher amount of KYN into the medium, indicating the enhanced tryptophan metabolism.ConclusionThe in vitro expanded primary human endometrial cells might be used as a model to study local immunoregulatory proteins IDO and RAGE involved in biology of endometrium.
Download or read book Culture of Epithelial Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: "...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." --Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Download or read book Biomarkers for Endometriosis written by Thomas D'Hooghe and published by Springer. This book was released on 2017-09-22 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of the diagnostic performance of non- or semi-invasive tests for endometriosis in peripheral blood, endometrium, saliva, peritoneal fluid and urine. The value of existing and emerging systems biology technologies for biomarker development is addressed in several chapters on genetics, microarrays, proteomics and metabolomics. Although tests with high sensitivity and acceptable specificity have been developed, sometimes validated in independent populations and seem promising, more research is needed to translate these data into clinical benefit for patients and coordinate efforts internationally to standarize analysis, reports and operating procedures. The gold standard to diagnose endometriosis is currently through laparoscopic inspection with histological confirmation, a surgical procedure with rare but significant potential risks for the patients. A non-invasive test for endometriosis would be critical for the early detection of endometriosis of symptomatic women with pelvic pain and/or subfertility with normal ultrasound. This would include nearly all cases of minimal-mild endometriosis, some cases of moderate-severe endometriosis without a clearly visible ovarian endometrioma and cases with pelvic adhesions and/or other pelvic pathology, who might benefit from surgery to improve pelvic pain and/or subfertility. Such a test would also be useful in symptomatic women with ultrasound imaging suspicious for endometriosis, since it may be difficult to differentiate an ovarian endometrioma from other ovarian cysts and since the quality of ultrasound imaging is highly variable worldwide.
Download or read book Regulation of Implantation and Establishment of Pregnancy in Mammals written by Rodney D. Geisert and published by Springer. This book was released on 2015-10-08 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past few decades technological advances in transcriptomics, proteomics, metabolomics, and glycomics along with the ability to selectively knockout genes of interest has greatly advanced our understanding of maternal-conceptus interactions that are essential for the establishment and maintenance of a successful pregnancy. This knowledge provides a foundation from which to build research endeavors to help resolve infertility, embryonic loss and recurrent abortion in humans, captive wild animals and important farm species. The present volume on “Regulation of Implantation and Establishment of Pregnancy in Mammals” brings together current reviews from leading experts to address the diversity of mechanisms by which species establish and maintain pregnancy. Implantation in rodents, dogs, pigs, cattle, sheep, horses, primates, humans and embryonic diapause in wild species are discussed. Reviews will provide current knowledge on the role of endometrial steroid receptors, adhesion factors, cytokines, interferons, steroids, prostaglandins, growth factors and immune cells involved with regulation of conceptus development.
Download or read book Molecular Research of Endometrial Pathophysiology written by Paola Viganò and published by MDPI. This book was released on 2019-10-14 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The endometrium has been the subject of intense research in a variety of clinical settings, because of its importance in the reproductive process and its role in women’s health. In the past 15 years, significant efforts have been invested in defining the molecular phenotype of the receptive phase endometrium as well as of various endometrial pathologies. Although this has generated a wealth of information on the molecular landscape of human endometrium, there is a need to complement this information in light of the novel methodologies and innovative technical approaches. The focus of this International Journal of Molecular Sciences Special Issue is on molecular and cellular mechanisms of endometrium and endometrium-related disorders. The progress made in the molecular actions of steroids, in the metabolism of steroids and intracrinology, in endometrial intracellular pathways, in stem cells biology, as well as in the molecular alterations underlying endometrium-related pathologies has been the focus of the reviews and papers included.]
Download or read book Neural Stem Cell Assays written by Navjot Kaur and published by John Wiley & Sons. This book was released on 2015-02-16 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages. Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, Neural Stem Cell Assays is a one-stop reference for consistent methods and reliable tools that span the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of neural stem cells in disease paradigms such as Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis. An excellent source of information for academic, pharmaceutical and biotechnology researchers who are new to the neural stem cell field, Neural Stem Cell Assays is an invaluable to experienced users who wish to integrate newly developed tools and technologies into their workflow. The book also covers important course material for students at the undergraduate and graduate level who are learning the basics of neural stem cell cultures, and differentiation to sub-neural lineages.
Download or read book The Primate Endometrium written by Carlo Bulletti and published by . This book was released on 1991 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Endometriosis written by Linda C. Giudice, MD and published by John Wiley & Sons. This book was released on 2012-01-18 with total page 625 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by an internationally well-known editor team, Endometriosis: Science and Practice is a state-of-the-art guide to this surprisingly common disease. While no cause for endometriosis has been determined, information of recent developments are outlined in this text, offering insight to improve management of symptoms medically or surgically. The first of its kind, this major textbook integrates scientific and clinical understanding of this painful disease helping to provide better patient care.
Download or read book Diagnosis and Management of Ovarian Disorders written by Albert Altchek and published by Elsevier. This book was released on 2003-09-04 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated second edition of Diagnosis and Management of Ovarian Disorders provides thorough, yet succinct insight into the ever-changing realm of ovarian disorders. It presents a novel multidisciplinary approach to the subject as described by clinicians, surgeons, pathologists, basic scientists and related medical researchers. Topics covered include reproductive technology, early diagnosis of ovarian cancer, and management of menopause among others. The breadth of information provided by this book will appeal to clinicians and researchers involved in the study and treatment of ovarian disorders. KEY FEATURES* Includes updated information on early diagnosis of ovarian cancer* Reviews new diagnostic techniques for ovarian disorders* Discusses latest information on reproductive technology* Presents translational treatment linking laboratory research with clinical medicine
Download or read book Histopathology of the Endometrium written by Gisela Dallenbach-Hellweg and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reevaluation of tumor classification, differential diagnosis and differential therapy based on modern knowledge. Revision of all chapters to incorporate new facts based on recent discoveries.
