Download or read book Two Dimensional Gel Electrophoresis of Proteins written by Julio Celis and published by Elsevier. This book was released on 2012-12-02 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications reviews current methods and clinical applications of two-dimensional gel electrophoresis of proteins, including the QUEST system, silver staining, and peptide mapping. Two-dimensional gel electrophoresis are applied to the study of diseases ranging from inborn errors of metabolism to human germ-line mutation rates, cancer, and mistranslation in animal and bacterial cells. This volume is organized into three sections encompassing 14 chapters and begins with an overview of the methodology of two-dimensional gel electrophoresis, followed by a discussion of computerized two-dimensional gel electrophoresis, silver staining, immunoblotting, and one- and two-dimensional peptide mapping. In most cases, a step-by-step guide to the techniques is given so that procedures may be easily repeated. A catalog of mouse fibroblast proteins is also given. The chapters that follow focus mainly on applications of two-dimensional gel electrophoresis in areas such as clinical and cancer research, human genetics, protein biosynthesis, and gene expression in plants. The final section presents current protein catalogs of Escherichia coli and human HeLa cells. This book is suitable for young researchers as well as for senior scientists working with a wide variety of problems in molecular and cell biology, basic biochemistry, genetics, and clinical research.
Download or read book Gel Electrophoresis of Proteins written by Michael J Dunn and published by Elsevier. This book was released on 2014-05-12 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.
Download or read book Protein Electrophoresis written by Biji T. Kurien and published by Humana Press. This book was released on 2012-05-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by . This book was released on 1981 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electrophoretic Techniques written by Colin F. Simpson and published by . This book was released on 1983 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immobilized PH Gradients Theory and Methodology written by P. G. Righetti and published by Elsevier. This book was released on 1990 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Download or read book Electrophoretic Separation of Proteins written by Biji T. Kurien and published by . This book was released on with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Download or read book Gel Electrophoresis of Proteins and Nucleic Acids written by Robert Carter Allen and published by . This book was released on 1994 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteome Research Two Dimensional Gel Electrophoresis and Identification Methods written by T. Rabilloud and published by Springer Science & Business Media. This book was released on 1999-09-17 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.
Download or read book 2 D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
Download or read book Mass Spectrometry for Microbial Proteomics written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2010-10-28 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Download or read book Protein Electrophoresis in Clinical Diagnosis written by David Keren and published by CRC Press. This book was released on 2003-09-26 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques,
Download or read book Electrophoresis in Practice written by Reiner Westermeier and published by John Wiley & Sons. This book was released on 2016-05-16 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Download or read book Gel Electrophoresis and Isoelectric Focusing of Proteins written by Robert C. Allen and published by Walter de Gruyter GmbH & Co KG. This book was released on 2019-05-20 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "Gel Electrophoresis and Isoelectric Focusing of Proteins".
Download or read book Protocols in Semen Biology Comparing Assays written by N. Srivastava and published by Springer. This book was released on 2017-09-19 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book on protocols in semen biology is a compilation of 20 chapters written by 15 experts from 5 Indian Council of Agricultural Research institutions, focusing on the basics of various procedures in semen biology with applications in animal and other allied sciences The information is presented in simple language with illustrative figures and colour microphotographs, making it understandable for readers of every level. It highlights recent findings, the comparative analysis of assays, protocols, points to ponder, background information and major references, and also compares various assays for evaluating a seminal parameters. The book provides a comprehensive resource for beginners, as well as academics, investigators and scientists of animal semen biology and relevant fields. Further, it offers valuable teaching material.
Download or read book Gel Electrophoresis of Proteins written by B. D. Hames and published by Oxford University Press, USA. This book was released on 1990 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers practical aspects involved in one and two dimensional gel electrophoresis, isoelectric focusing, immunoelectrophoresis, and peptide mapping. Distributed by Oxford U. Press. Annotation copyrighted by Book News, Inc., Portland, OR
