Download or read book Fundamental Studies in High Temperature and Reversed Phase Liquid Chromatography written by Jeffrey Alan Graham and published by . This book was released on 1979 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High Temperature Liquid Chromatography written by Thorsten Teutenberg and published by Royal Society of Chemistry. This book was released on 2010-06-03 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-temperature liquid chromatography has attracted much interest in recent years but has not yet recognized its full potential in the chromatographic community. There is a widespread reluctance in industry to use temperature to speed up the separation process, influence the selectivity of a separation or implement novel detection techniques. However, the technology has now matured and could revolutionize chromatography as we see it today. Better equipment, such as heating systems able to generate faster heating rates, is becoming more readily available. Also, columns based on silica gel, which can withstand higher temperatures for an extended period, are now being introduced. Nevertheless, further technological and methodical efforts are needed to establish the method in a regulated environment like the pharmaceutical industry. This is the only text to cover all the practical aspects, as well as the underlying theoretical principles, of setting up an HPLC system for high temperature operation. It is not intended solely for academics but will also benefit the researcher interested in more practical considerations. The author is a recognized expert and has conducted several studies with partners from industry to validate the method. Many real examples from these studies have been included in the book. The aim is to support practitioners in the creation of their own protocols without the need to rely solely on trial and error. The book starts with a brief definition of high temperature liquid chromatography before going on to cover: system set up; the heating system; mobile phase considerations; suitable stationary phases; method development using temperature programming; analyte stability, and special hyphenation techniques using superheated water as a mobile phase. In each chapter, experimental data is used to illustrate the main statements and the advantages over conventional HPLC are evaluated. The book concludes with a critical outlook on further developments and applications underlining the necessary advances needed to make high temperature HPLC more robust.
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-22 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Download or read book High Performance Liquid Chromatography written by W.J. Lough and published by CRC Press. This book was released on 1995-09-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1992 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High Performance Liquid Chromatography written by Csaba Horváth and published by Elsevier. This book was released on 2013-10-22 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Performance Liquid Chromatography: Advances and Perspectives, Volume 2 presents the fundamental aspects of high-performance liquid chromatography, laboratory technique for chemical analysis with a wide range of applications. The book consists of three chapters discussing the optimization of the column and the operating conditions of the chromatographic system; use of polar adsorbents and nonpolar eluents; and reversed-phase chromatography, the main branch of high-performance liquid chromatography. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good reference material.
Download or read book Coupling Temperature Control with Electrochemically Modulated Liquid Chromatography written by Lisa M. Ponton and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The primary focus of the doctoral research presented herein has been the integration of temperature control into electrochemically modulated liquid chromatography (EMLC). The combination of temperature control and the tunable characteristics of carbonaceous EMLC stationary phases have been invaluable in deciphering the subtleties of the retention mechanism. The effects of temperature and E[subscript app] on the retention of several naphthalene disulfonates were therefore examined by the van't Hoff relationship. The results indicate that while the retention of both compounds is exothermic at levels comparable to that in many reversed-phase separations, the potential dependence of the separation is actually entropically affected in a manner paralleling that of several classical ion exchange systems. Furthermore, the retention of small inorganic anions at constant temperature also showed evidence of an ion exchange type of mechanism. While a more complete mechanistic description will come from examining the thermodynamics of retention for a wider variety of analytes, this research has laid the groundwork for full exploitation of temperature as a tool to develop retention rules for EMLC. Operating EMLC at elevated temperature and flow conditions has decreased analysis time and has enabled the separation of analytes not normally achievable on a carbon stationary phase. The separation of several aromatic sulfonates was achieved in less than 1 min, a reduction of analysis time by more than a factor of 20 as compared to room temperature separations. The use of higher operating temperatures also facilitated the separation of this mixture with an entirely aqueous mobile phase in less than 2 min. This methodology was extended to the difficult separation of polycyclic aromatic hydrocarbons on PGC. This study also brought to light the mechanistic implications of the unique retention behavior of these analytes through variations of the mobile phase composition.
Download or read book High Performance Liquid Chromatography written by Mary T. Gilbert and published by Elsevier. This book was released on 2013-10-22 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.
Download or read book Fundamental Studies and Applications of Reversed phase Liquid Chromatographic Methods of Analysis written by Chun-I. Kong and published by . This book was released on 1980 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book High Performance Liquid Chromatography written by Phyllis R. Brown and published by Wiley-Interscience. This book was released on 1989-01-19 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detectors for Liquid Chromatography Edited by Edward S. Yeung Written by an expert in the field, this comprehensive guide explains the basic principles behind detector response instrumentation and selected applications. Early chapters cover absorption detectors for high performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, and polarimetric detectors. Coverage continues with detection based on electrical and electrochemical measurements, mass spectrometry as an online detector for HPLC, and miscellaneous methods. 1986 0 471-82169-1 366 pp. Small Bore High Performance Liquid Chromatography Edited by Raymond R. W. Scott A state-of-the-art guide that demonstrates how to design, construct, and pack optimized small bore columns, the center of any chromatography system. Case examples show the use of these columns for high resolution, very fast analysis, and special methods for molecular weight determinations. Applications from a wide range of industrial and forensic analyses aid in developing sophistication in a number of useful techniques. The book provides essential information on topics such as calculating the minimum column radius, detectors, and molecular diffusion. Includes 92 illustrations and 14 tables to enhance explanations of microbore HPLC methods. 1984 0 471-80052-X 271 pp. Reversed-Phase High Performance Liquid Chromatography: Theory, Practice, and Biomedical Applications Ante M. Krstulovic and Phyllis R. Brown Reversed-phase liquid chromatography has increased tremendously in popularity over the past ten years. Estimates show that more than 80% of all HPLC separations are performed using this technique. This book covers both theoretical aspects of RPLC and practical information needed in diverse areas of research; it also contains a review of the RPLC applications in the biomedical/biochemical field, with references and collateral readings. Material is presented in a practical, problem-solving manner and should be immensely useful in theoretical aspects of RPLC and all areas of scientific research, particularly the biomedical/biochemical field, where RPLC has made its largest impact. 1982 0 471-05369-4 296 pp.
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-23 with total page 840 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Download or read book Chromatography written by E. Heftmann and published by Elsevier. This book was released on 2004-04-16 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis. In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated. 1. Each chapter written by an authority2. Thorough treatment of the theoretical basis of separation methods3. Practical guide for performing analyses
Download or read book High performance Liquid Chromatography Reversed phase chromatography of proteins and nucleic acids pratical considerations written by Csaba Horváth and published by . This book was released on 1980 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book HPLC written by Joel K. Swadesh and published by CRC Press. This book was released on 1996-11-26 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) is a technique used in diverse laboratory and industrial settings for the separation of components of complex mixtures. HPLC: Practical and Industrial Applications is a trouble-shooting, problem-solving guide for scientists, engineers, and technicians who use HPLC in their day-to-day work. It provides the answers to specific problems and includes practical case studies. This case history approach to chromatography is an effective teaching tool and clearly illustrates how to use techniques such as reversed phase chromatography, ion exchange chromatography, gel permeation chromatography, and capillary electrophoresis. The book is organized to facilitate rapid understanding for those working with a particular area of chromatography: Introduces the reader to instrumentation, reviews basic chromatographic theory, and presents a brief survey of absorbance fluorescence and refractive index detectors. Provides a broad view of the role of the analytical laboratory in an industrial organization. Offers suggestions on optimizing the utilization of personnel and work flow in the laboratory. Covers process sampling and analysis · Describes process chromatography. The remaining chapters discuss specialties within separations technology, including an outline of the key features of each technique, a thorough bibliography, a list of precedents, and detailed examples of one or more applications presented from the viewpoint of industrial and basic scientists. Specialty detectors are also described. HPLC: Practical and Industrial Applications is an essential reference for those working in the industrial sector, as well as scientists, students, technicians interested in learning HPLC methods.
Download or read book High Performance Gradient Elution written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2007-01-09 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.
Download or read book Reversed phase High performance Liquid Chromatography written by H. B. Patel and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Reversed Phase High Performance Liquid Chromatography written by Ante M. Krstulovic and published by . This book was released on 1982-05-04 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive problem-solving approach to reversed-phase high-performance liquid chromatography covering the theoretical aspects and practical information needed in diverse areas of research. Also reviews RPLC applications in the biomedical/biochemical field.
