Download or read book Fundamental Laboratory Approaches for Biochemistry and Biotechnology written by Alexander J. Ninfa and published by John Wiley & Sons. This book was released on 2009-05-26 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Download or read book Fundamental Laboratory Approaches for Biochemistry and Biotechnology written by Alexander J. Ninfa and published by . This book was released on 2010 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written as a biochemistry laboratory text for advanced students in biochemistry and other life sciences. It provides a logical framework on how to approach research problems and how to conduct and evaluate scientific research.
Download or read book Basic Laboratory Methods for Biotechnology written by Lisa A. Seidman and published by CRC Press. This book was released on 2021-12-29 with total page 1210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
Download or read book Advanced Methods in Molecular Biology and Biotechnology written by Khalid Z. Masoodi and published by Academic Press. This book was released on 2020-10-28 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Download or read book Quickstart Molecular Biology written by Philip N. Benfey and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book is an introductory course in molecular biology for mathematicians, physicists, and engineers. It covers the basic features of DNA, proteins, and cells but in the context of recent technological advances, such as next-generation sequencing and high-throughput screens, and their applications. This enables readers to move rapidly from the b
Download or read book Microbial Physiology and Biochemistry Laboratory written by David White and published by Oxford University Press on Demand. This book was released on 1998 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Physiology and Biochemistry Laboratory illustrates the major features of growth and metabolism discussed in David White's The Physiology and Biochemistry of Prokaryotes (OUP, 1995). It serves as an ideal adjunct to this text and can also be used in conjunction with other books for the laboratory component of a microbial physiology course. All of the experiments described in this manual have been taught as part of a laboratory course for junior and senior biology and microbiology majors at Indiana University. In addition to reinforcing what students learn in lecture, the experiments guide students through a wide spectrum of analytical techniques including enzyme assays, macromolecular assays, column chromatography, gel electrophoresis, and gas chromatography. Along with enzyme assays and enzyme purification, students do experiments measuring oxygen uptake, chemotaxis, fermentation, and bacterial luminescence. The organisms studied include Escherichia, Pseudomonas, Bacillus, Proteus, Rhodospirillum, Photobacterium, and Saccharomyces. The volume is enhanced by appendices which include sections on quantitative problems and their solutions, instructions on how to write a laboratory report, and independent projects that are extensions of the class experiments. The number of experiments exceeds the amount of material usually offered in one semester, giving instructors the option to choose those experiments that are most appropriate for their classes.
Download or read book Molecular Biology Techniques written by Heather Miller and published by Academic Press. This book was released on 2011-10-18 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Download or read book Principles and Techniques of Biochemistry and Molecular Biology written by Keith Wilson and published by Cambridge University Press. This book was released on 2010-03-04 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Download or read book Recent Advances in Plant Biotechnology written by Ara Kirakosyan and published by Springer Science & Business Media. This book was released on 2009-08-15 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biotechnology applies to three major areas of plants and their uses: (1) control of plant growth and development; (2) protection of plants against biotic and abiotic stresses; and (3) expansion of ways by which specialty foods, biochemicals, and pharmaceuticals are produced. The topic of recent advances in plant biotechnology is ripe for consideration because of the rapid developments in this ?eld that have revolutionized our concepts of sustainable food production, cost-effective alt- native energy strategies, environmental bioremediation, and production of pla- derived medicines through plant cell biotechnology. Many of the more traditional approaches to plant biotechnology are woefully out of date and even obsolete. Fresh approaches are therefore required. To this end, we have brought together a group of contributors who address the most recent advances in plant biotechnology and what they mean for human progress, and hopefully, a more sustainable future. Achievements today in plant biotechnology have already surpassed all previous expectations. These are based on promising accomplishments in the last several decades and the fact that plant biotechnology has emerged as an exciting area of research by creating unprecedented opportunities for the manipulation of biological systems. In connection with its recent advances, plant biotechnology now allows for the transfer of a greater variety of genetic information in a more precise, controlled manner. The potential for improving plant productivity and its proper use in agric- ture relies largely on newly developed DNA biotechnology and molecular markers.
Download or read book Physical Biochemistry written by David Sheehan and published by John Wiley & Sons. This book was released on 2013-04-30 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: "As will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic...This is a text to be welcomed by both teachers and students." BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with many years teaching and research experience, this text is a must-have for students of biochemistry, biophysics, molecular and life sciences and food science.
Download or read book Molecular Biotechnology written by Bernard R. Glick and published by . This book was released on 1998 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
Download or read book Biotechnology Fundamentals written by Firdos Alam Khan and published by CRC Press. This book was released on 2015-11-18 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: A single source reference covering every aspect of biotechnology, Biotechnology Fundamentals, Second Edition breaks down the basic fundamentals of this discipline, and highlights both conventional and modern approaches unique to the industry. In addition to recent advances and updates relevant to the first edition, the revised work also covers ethics in biotechnology and discusses career possibilities in this growing field. The book begins with a basic introduction of biotechnology, moves on to more complex topics, and provides relevant examples along the way. Each chapter begins with a brief summary, is illustrated by simple line diagrams, pictures, and tables, and ends with a question session, an assignment, and field trip information. The author also discusses the connection between plant breeding, cheese making, in vitro fertilization, alcohol fermentation, and biotechnology. Comprised of 15 chapters, this seminal work offers in-depth coverage of topics that include: Genes and Genomics Proteins and Proteomics Recombinant DNA Technology Microbial Biotechnology Agricultural Biotechnology Animal Biotechnology Environmental Biotechnology Medical Biotechnology Nanobiotechnology Product Development in Biotechnology Industrial Biotechnology Ethics in Biotechnology Careers in Biotechnology Laboratory Tutorials Biotechnology Fundamentals, Second Edition provides a complete introduction of biotechnology to students taking biotechnology or life science courses and offers a detailed overview of the fundamentals to anyone in need of comprehensive information on the subject.
Download or read book Fundamentals of Enzyme Kinetics written by Athel Cornish-Bowden and published by Elsevier. This book was released on 2014-05-20 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.
Download or read book Methods in Applied Soil Microbiology and Biochemistry written by Kassem Alef and published by Academic Press. This book was released on 1995-07-11 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quality control and quality assurance in applied soil microbiology and biochemistry. Soil sampling, handling, storage and analysis. Enrichment, isolation and counting of soil microrganisms. Anaerobic microbial activities in soil. Enzyme activities. Microbial biomass. Community structure. Field methods. Bioremediation of soil.
Download or read book Live Cell Imaging written by Robert D. Goldman and published by CSHL Press. This book was released on 2005 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Download or read book Biochemistry written by Jeremy M. Berg and published by W. H. Freeman. This book was released on 2015-04-08 with total page 1120 pages. Available in PDF, EPUB and Kindle. Book excerpt: For four decades, this extraordinary textbook played an pivotal role in the way biochemistry is taught, offering exceptionally clear writing, innovative graphics, coverage of the latest research techniques and advances, and a signature emphasis on physiological and medical relevance. Those defining features are at the heart of this edition. See what's in the LaunchPad
