Download or read book Functional Characterization of Prostate Cancer Biomarkers written by Josefine Gerhardt and published by . This book was released on 2011 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of Three Novel Candidate Biomarkers and Therapeutic Targets for Prostate Cancer written by Yu Zhang and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Functional Characterisation of Prostate Cancer Biomarkers written by Matthew Daniel Parker and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of JAG1 in Prostate Cancer written by and published by . This book was released on 2016 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of Common Prostate Cancer Risk Variants written by Xing Xu and published by . This book was released on 2014 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of the Cyclin K Protein in Prostate Cancer written by Sabrina Schecher and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of the Prostate Cancer Progression Marker MiR 375 written by Julia Romic-Pickl and published by . This book was released on 2016* with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Functional Characterization of a Long Non coding RNA Associated with Prostate Cancer written by Md Faqrul Hasan and published by . This book was released on 2019 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the most common cancer in men in the western world. Although early stage prostate cancer is treatable late stage, more specifically, metastatic and drug resistant prostate cancers are mostly incurable. The failure of current treatments obligates the research community to explore novel areas in prostate cancer biology and find better therapeutic targets. Emerging evidences show that non-coding RNAs specifically long non-coding RNAs (lncRNAs) play regulatory roles in various cellular processes and are frequently dysregulated in cancer including prostate cancer. These aberrantly expressed lncRNAs mostly with unexplored genetic information may drive cancer progression. Previous studies done in our laboratory showed a tumor suppressor role of a cluster of small non-coding RNAs or microRNA (miRNA) miR-17-92a in PC-3 prostate cancer cells. To learn the underlying mechanism, transcriptome analysis with or without expression of miR-17-92a was conducted in our laboratory. RNA-sequencing data analysis identified reduced expression of a set of lncRNAs and oncogenes, and up regulation of several tumor suppressor genes upon expression of miR-17-92a cluster miRNAs. One of the down regulated intergenic lncRNAs, PAINT (Prostate Cancer Associated Intergenic Non-coding Transcript) (LINC00888), was selected for determining its functional role in prostate cancer. TCGA and GEO profiles analyses revealed up regulation of PAINT in prostate tumors with higher Gleason Scores, in highly aggressive metastatic prostate cancer cell lines, and upon androgen deprivation therapy of prostate cancer cells.

Download or read book Identification and Functional Characterization of Novel Markers for Prostate Cancer written by Alexander Hermani and published by . This book was released on 2005 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of Two Novel Human Prostate Cancer Metastasis Related Genes written by and published by . This book was released on 2005 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: We propose to identify the functional characterization of two novel cancer-specific, metastasis-related genes whose constitutive expression may be pivotal for prostate cancer progression. Work accomplished was performed based on the proposed statement of work. We have characterized the full-length cDNAs of the Seq1 and Seq2 genes using at least three 5' and '3 rapid amplifications of cDNA ends (RACE) commercial kits (Invitrogen Carlsbad, CA, BD Bioscience (Clontech Inc), and Seegene, Rockville, MD). To optimize the PCR conditions for each kit, we had designed several sets of gene-specific primers (GSP;23-28 nt long) with 50-70% GC and Tm of 55 to 75 degrees C for each gene. We have also designed several sets of nested GSPs to verify our cloned genes. Because of unique secondary structures, high GC content, short SSH sequences, and low levels of expression of these genes in prostate cancer cell lines, we had great deal of difficulty in accomplishing this task in a timely fashion. As such, we devised different strategies for the first-strand synthesis using a modified oligo(dT) primers (5'-CDS primer or 3'-CDS primer), and Smart oligo II primer under various conditions. The full-length cDNA sequences were subcloned into mammalian expression vectors (Invitrogen) and ready to be used for generation of recombinant proteins and antibody production.

Download or read book Differentially Expressed Proteins in Prostate Cancer and Functional Characterization of Proteins with Altered Expression written by Ramesh Ummanni and published by . This book was released on 2008 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book MiRNAs and Prostate Cancer written by Annika Fendler and published by . This book was released on 2011 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Prostate Cancer Associated Protein Biomarkers Using High Throughput Mass Spectrometry written by and published by . This book was released on 2006 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer (PCa) remains to be the most common non-skin cancer in the US. Currently available screening tests for PCa including prostate specific antigen (PSA) test, digital rectal examination (DRE) and prostate biopsy, call for more accurate and non-invasive techniques to detect, diagnose, and stratify the disease based on molecular markers present in the body fluids. Using MALDI-TOF mass spectrometry protein fingerprint profiling, we generated decision tree algorithms to classify cancer from non-cancer. We have also devised strategies to isolate and identify protein biomarkers from the fingerprint profiles of PCa patients in the clinical gray-area where PSA fails to detect cancer. Identification of such cancer biomarkers will assist in development of better non-invasive diagnostic tools for prostate cancer and may also lead to better therapeutic targets.

Download or read book The Unconventional Role of Akt1 in Advanced Prostate Cancer written by Abdulrahman Mohammed Alwhaibi and published by . This book was released on 2019 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Analysis of Risk Single Nucleotide Polymorphism and Long Noncoding RNA in Prostate Cancer written by Jun Jie Hua and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Prostate cancer is the most commonly diagnosed non-cutaneous cancer in men worldwide. While most low-risk prostate cancer patients have a favourable clinical outcome, intermediate- and high-risk patients with aggressive forms of the disease often relapse despite the initially effective local and conventional androgen-deprivation therapies. Therefore, two main challenges in the field of prostate cancer are patient stratification into appropriate risk groups through prognostic biomarkers and novel therapeutic options for patients with aggressive prostate cancer. Prostate cancer has a strong genetic component, with Genome-wide Association Studies (GWAS) identifying over 150 risk single nucleotide polymorphisms (SNPs). While these risk SNPs are associated with prostate cancer predisposition and a few also associated with disease progression, their functional mechanisms remain elusive. Here, I evaluated the association of the risk SNP rs11672691 with prostate cancer progression and its clinical relevance, and uncovered its regulatory mechanisms at a novel bifunctional enhancer-promoter element to dysregulate two gene isoforms of a long noncoding RNA (lncRNA) PCAT19. Furthermore, I validated the oncogenic properties of the lncRNA PCAT19, and revealed its function in prostate cancer progression through the upregulation of a subset of cell cycle genes with the RNA binding protein HNRNPAB. My work signifies the clinical importance in understanding the functional mechanisms of prostate cancer risk SNPs and lncRNAs in prostate cancer. Additionally, my work also shed light on the function and molecular mechanisms of a novel class of regulatory elements in the human genome, and its role in cancer aggressiveness.

Download or read book Clinic and Functional Analysis of P73R1 Mutations in Prostate Cancer written by and published by . This book was released on 2006 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: The DNA damage-signaling pathway has been implicated in the development of prostate cancer since germline mutations in several genes (BRCA1, BRCA2, and CHEK2) whose products are involved in this pathway have been associated with increased risk for this cancer. We previously isolated a novel p73 up-regulated gene (p73R1) and identified p73R1 mutations in prostate cancer. In this report, we screened 856 unselected prostate cancer specimens and detected a frequency of 2.6% (221856) truncation mutations in prostate cancers in contrast to 0.6% (21327) in 327 population-based controls (Fishers exact test, P = 0.036), with an odds ratio of 4.3 (95% confidence interval 1.2 - 21.2). In addition, we also demonstrated that mutant p73R1 was unable to induce apoptosis and suppress cell growth in HeLa and Cos7 cells. The loss of function mutation in p73R1 is due to the inability of the mutant to induce cytochrome c release from mitochondria. These results suggest that loss of function mutations in p73R1 predispose men to prostate cancer and further support the concept that the genetic defects in the DNA damage-response genes play an important role in the development of prostate cancer.

Download or read book Molecular Biology of Prostate Cancer written by Manfred Wirth and published by Walter de Gruyter. This book was released on 2013-05-22 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: