Download or read book Functional Characterization of a Puf Protein Family Member in Malaria Parasite Plasmodium Falciparum written by Xiaoying Liang and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is important to cellular growth and differentiation of eukaryotes, as it plays key roles in determining the eventual expression levels and cellular localizations of a large number of proteins. RNA-binding proteins (RBPs) with conserved RNA-binding domains (RBDs) are key molecules in controlling gene expression. These conserved RBDs can specifically interact with target RNAs through specific sequences and structures to regulate mRNA stability, translation efficiency and subcellular localization. One of the well-studied RBPs is the Puf family RBPs. Members of the Puf family share a highly conserved Puf RBD, which typically consists of eight imperfect tandem repeats of ~36 amino acids (aa). Here I report the studies of two Puf members, PfPuf3 in Plasmodium falciparum and its ortholog PyPuf3 in Plasmodium yoelii. Both genes could not be disrupted, suggesting that they may be essential for the intraerythrocytic developmental cycle (IDC). A time-course study of both PfPuf3 mRNA and protein indicated that PfPuf3 was expressed during the entire IDC, with peak expression in early trophozoites. Cellular fractionation demonstrated that PfPuf3 preferentially partitioned to the nuclear than the cytoplasmic fractions. Interestingly, tagging of the endogenous of PfPuf3 and PyPuf3 with the green fluorescent protein showed that both proteins were limited to the nucleus, which is in contrast to the cytoplasmic localization of PfPuf1 and PfPuf2. Further, we found PfPuf3 co-localized with a well-known nucleolus maker PfNop1, demonstrating that PfPuf3 is a nucleolar protein. Using a parasite line with PfPuf3-tagged with PTP (ProtC-TEV-ProtA), we were able to affinity purify the PfPuf3 protein complex and the associated RNAs. Proteomic analysis of the PfPuf3 complex identified 32 proteins that are associated with the 60S ribosome subunit, suggesting that PfPuf3 might be involved in ribosomal biogenesis. RNA sequencing analysis of the purified PfPuf3 complex revealed significant enrichment of the 28S rRNA and ITS2 (internal transcribed spacer 2), implying that PfPuf3 may recognize and bind to these rRNA sequences. Taken together, these results demonstrated nucleolar localization of PfPuf3 and suggested an essential function of PfPuf3 in ribosomal biogenesis.

Download or read book written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Functional Characterization of Two Putative Telomere associated Proteins in the Malaria Parasite Plasmodium Falciparum written by Ajuh Elvis Tasih and published by . This book was released on 2013 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of the RIFIN Protein Family of the Malaria Parasite Plasmodium Falciparum Welch 1897 written by Michaela Petter and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Malaria Drugs Disease and Post genomic Biology written by David Sullivan and published by Springer Science & Business Media. This book was released on 2006-01-09 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite rapid increases in knowledge, malaria continues to kill more than a million people each year and causes symptomatic disease in a further 300 million individuals. This volume brings some of the world's best investigators to describe recent advances in both the scientific and clinical aspects of malaria, and bridges between the two.

Download or read book Toxoplasma Gondii written by Louis M. Weiss and published by Elsevier. This book was released on 2011-04-28 with total page 801 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxoplasmosis is caused by a one-celled protozoan parasite known as Toxoplasma gondii. In the United States, it is estimated that approximately 30% of cats, the primary carriers, have been infected by T. gondii. Most humans contract toxoplasmosis by eating cyst-contaminated raw or undercooked meat, vegetables, or milk products or when they come into contact with the T. gondii eggs from cat feaces while cleaning a cat's litterbox, gardening, or playing in a sandbox. Approx 1 in 4 (more than 60 million) people in the USA are infected with the parasite, and in the UK between 0.5 and 1% of individuals become infected each year. By the age of 50, 40% of people test positive for the parasite. The predilection of this parasite is for the central nervous system (CNS) causing behavioral and personality alterations as well as fatal necrotizing encephalitis, and is especially dangerous for HIV infected patients. Though there have been tremendous strides in our understanding of the biology of Toxoplasma gondii in the last decade, there has been no systemic review of all of the information that has accumulated. Toxoplasma gondii provides the first comprehensive summary of literature on this organism by leading experts in the field who were responsible for organising the 7th International Congress on Toxoplasmosis in May 2003. It offeres systematic reviews of the biology of this pathogen as well as descriptions of the methods and resources used. Within the next year the T. gondii genome will be completed making this an indispensable research resource for biologists, physicians, parasitologists, and for all those contemplating experiments using T. gondii. * Serves as a model for understanding invasion of host cells by parasites, immune response, motility, differentiation, phylogenetics, evolution and organelle acquisition * Discusses the protocols related to genetic manipulation, cell biology and animal models while also providing reference material on available resources for working with this organism

Download or read book Yeast Gene Analysis written by and published by Elsevier. This book was released on 2011-09-21 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence. New chapters on: Strain construction; genome-wide two-hybrid approaches; use of microarrays for transcript analysis; real-time analysis of chromosome behaviour and FRET; synthetic gene array technology and protein arrays; chemical genomics and yeast prions; RNA gene analysis and mitochondrial gene function analysis; phylogenetic footprinting; discovering human gene function and predicting yeast gene function

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 946 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Basis of Stage Conversion in Apicomplexan Parasites written by Maria E. Francia and published by Frontiers Media SA. This book was released on 2021-06-08 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Carrier Proteins Advances in Research and Application 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carrier Proteins—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Carrier Proteins. The editors have built Carrier Proteins—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Carrier Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Carrier Proteins—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Malaria Parasites written by Andrew P. Waters and published by Caister Academic Press Limited. This book was released on 2004 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: The completion of the Plasmodium falciparum genome sequence in late 2002 heralded a new era in malaria research. The search began in earnest for new drugs and vaccines to combat malaria, a disease which afflicts up to 500 million people worldwide and is responsible for the deaths of more than one million people each year. The new genomic data is aiding a greater understanding of the living parasite and its interaction with the insect vector and human host. In this book internationally renowned experts provide up-to-date reviews of the most important aspects of post-genomic malaria research. Topics covered include: the P. falciparum genome and model parasites, bioinformatics and genome databases, microsatellite analysis, analysis of chromosome structure, cell cycle to RNA polymerase I and II mediated gene expression, role of the nuclear genome, the parasite surface and cell biology, and much more. The book is essential to all researchers working in this highly topical field and is recommended reading for scientists in other areas of biology and medicine.

Download or read book Parasite Infections From Experimental Models to Natural Systems written by Toni Aebischer and published by Frontiers Media SA. This book was released on 2018-07-06 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic parasites (including parasitic protozoans, worms and arthropods) are more complex and heterogeneous organisms than pathogenic bacteria and viruses. This notion implies different evolutionary strategies of host exploitation. Typically, parasites establish long-term infections and induce relatively little mortality, as they often limit pathological changes by modulating host cells and downregulating adverse immune responses. Their pattern of distribution tends to be endemic rather than epidemic. Despite these seemingly benign traits, parasites usually cause substantial chronic morbidity, thus constituting an enormous socioeconomic burden in humans, particularly in resource poor countries, and in livestock worldwide. Parasite-induced fitness costs are an evolutionary force that can shape populations and contribute to species diversity. Therefore, a thorough understanding of parasites and parasitic diseases requires detailed knowledge of the respective biochemical, molecular and immunological aspects as well as of population genetics, epidemiology and ecology. This Research Topic (RT) bridges disciplines to connect molecular, immunological and wildlife aspects of parasitic infections. The RT puts emphases on four groups of parasites: Plasmodium, Toxoplasma, Giardia and intestinal helminths. Co-infections are also covered by the RT as they represent the most common form of parasite infections in wildlife and domestic animal populations. Within the four types of parasites the following topics are addressed: (1) Experimental models: hypothesis testing, translation and limits. (2) Critical appraisal of experimental models. (3) Natural systems: Technological advances for investigations in natural parasite-host systems and studies in natural systems. (4) The urgent need for better models and methods in natural parasite systems. Hence, the RT covers and illustrate by the means of four main parasitic infections the parasite-host system at the molecular, cellular and organismic level.

Download or read book Current Topics in Malaria written by Alfonso J. Rodriguez-Morales and published by . This book was released on 2016-11-30 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advances in Malaria Research written by Deepak Gaur and published by John Wiley & Sons. This book was released on 2016-11-30 with total page 615 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thoroughly reviews our current understanding of malarial biology Explores the subject with insights from post-genomic technologies Looks broadly at the disease, vectors of infection, and treatment and prevention strategies A timely publication with chapters written by global researchers leaders

Download or read book Index Medicus written by and published by . This book was released on 2003 with total page 1696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Download or read book Molecular Parasitology written by Julia Walochnik and published by Springer. This book was released on 2016-10-20 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past years, genome projects for numerous human parasites have been completed and now allow first in depth comparisons and evolutionary conclusions. The genomes of parasites reflect the coevolution with their host, metabolic capacities depending on their respective habitat in the host. Gut parasites usually have an anaerobic metabolism, while blood parasites have an aerobic metabolism, intracellular parasites escape the immune system, while extracellular parasites evade the immune system, usually by antigenic variation. Comprehensive genome data now being available allow us to address profound scientific questions, such as which traits enable the parasite to survive in the human host, which to cause disease and which can be used as drug targets. This book intends to give an overview of the state of knowledge on “the molecules” of protozoan parasites – on their genomes, proteomes, glycomes and lipidomes.

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1990 with total page 1476 pages. Available in PDF, EPUB and Kindle. Book excerpt: