Download or read book Functional and Structural Studies of Cytochrome P450 by Resonance Raman Spectroscopy written by Tian-jing Deng and published by . This book was released on 1999 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Guide to Cytochromes P450 written by David F. V. Lewis and published by CRC Press. This book was released on 2001-04-12 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochromes are coloured iron-containing proteins that transfer electrons during cellular respiration and photosynthesis. The Cytochrome P450 family of enzymes catalyze reactions whereby water-insoluble drugs or metabolites, that would otherwise reach toxic levels in cell membranes, are rendered suitably water-soluble to leave the cell and be excreted in the urine. Due to the extensive nature of this subject, which is an area of intense scientific interest, the field is rapidly advancing and there is a need for new textbooks to keep abreast of the latest developments. The book fulfils that role in providing a fast-track approach for those coming into the P450 field, either at postgraduate level or in particular within the pharmaceutical industry. A Guide to Cytochrome P450 Structure and Function acts as an adjunct to the previous book Cytochromes P450: Structure, Function and Mechanism. It reviews the current status of the P450 field in terms of our present knowledge and understanding of the enzymes structure and function, including their multiplicity of forms, diversity of substrates, and selectivity. This is brought together with the latest research topics, including pharmacogenetics, regulation, human DMEs, toxicity screening and molecule modeling, to provide a fast-track approach for those new to the field.
Download or read book Guide to Cytochromes written by David F.V. Lewis and published by CRC Press. This book was released on 1996-07-03 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This guide to the structure, function and mechanism of the cytochromes P450 focuses on the role of P450s in xenobiotic metabolism and toxicity. Colour illustrations show how modelling of P450s can rationalize their substrate specificity for the metabolism of both endogenous and exogenous chemicals.
Download or read book Proteins of the Cytochrome P 450 Monooxygenase System and Cytochrome C written by Kim Kelly and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteins of the Cytochrome P 450 Monoonygenase System and Cytochrome C written by Kim Kelly and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cytochrome P450 2D6 written by Shufeng Zhou and published by CRC Press. This book was released on 2018-09-03 with total page 1171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochromes are proteins that catalyze electron transfer reactions of well-known metabolic pathways and are classified in various superfamilies. The CYP, or P450, superfamily accounts for 90% of the oxidative metabolism of clinical drugs. One member of this superfamily, P450 2D6 (or CYP2D6), singlehandedly metabolizes about 25% of all medications in the human liver. Cytochrome P450 2D6: Structure, Function, Regulation, and Polymorphism reviews the current knowledge of CYP2D6 as well as the maturing body of evidence indicating its significance to clinical and pharmacological researchers and practitioners. This book focuses on the critical role CYP2D6 plays in the human liver. It examines the genetic, epigenetic, physiological, pathological, and structural factors of the gene that govern the highly variable metabolism of a number of drugs in clinical use. It highlights the impact of the functional roles of CYP2D6 on clinical practice and drug development and also discusses implications for precise medicine, strategies to avoid adverse drug reactions, and paths for future research. Cytochrome P450 2D6 is a unique, valuable book focusing on a single but immensely powerful human gene. It provides the first single source of comprehensive information on CYP2D6 that serves as an important reference for medical, biomedical, pharmaceutical, and nursing researchers, practitioners, and students.
Download or read book Vibrational Spectroscopy in Protein Research written by Yukihiro Ozaki and published by Academic Press. This book was released on 2020-05-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.
Download or read book Cytochrome P450 written by Paul R. Ortiz de Montellano and published by Springer Science & Business Media. This book was released on 2005 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of this title in 1986, the cytochrome P450 field has completed the transition to a discipline in which structure and mechanism, even regulation and biological function, are dealt with in molecular terms. The present updated edition has dropped some of the chapter topics from the first edition to make room for new or expanded topics. The volume is divided into four sections: model systems; structurally defined bacterial enzymes; structures and mechanisms of membrane-bound P450 enzymes; and regulatory mechanisms and physiological roles of cytochrome P450.
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structure function Studies of Human Cytochrome P450 2C9 30 Genetic Variant written by Sunghwan Cho and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochrome P450 (CYP) enzymes are a superfamily of heme-containing proteins found on the membrane of the endoplasmic reticulum. CYPs constitute the major enzyme family in drug metabolism with increasing importance in pharmacogenetics. The human CYP2C9 enzyme, which is responsible for the metabolism of over 20% of available clinical drugs, is highly polymorphic. More than 80 genetic variants of this enzyme have been identified, with many exhibiting significantly altered activities toward various medications compared to the wild type (WT). Despite the extensive functional and structural studies of various CYPs from different species, the fundamental basis of genetic polymorphisms is scant. The CYP2C9*30 is a rare allelic variant, which is found in Japanese population. It represents an amino acid substitution at position 477 from alanine to threonine that has resulted in significantly altered activity of the enzyme towards various substrates compared to the WT. We have expressed and purified the CYP2C9*30 variant in E.coli to the optimal quality. The in-vitro enzymatic assays revealed reduced activity and substrate turn-over by CYP2C9*30 compared to the WT. The previously solved crystal structure of the CYP2C9*30-losartan complex demonstrated the reorientation of amino acid side-chain of glutamine at 214 or Q214 that interacted with the polar substitution threonine at 477 or A477T in the *30 structure. The goal is to investigate the activity of CYP2C9*30, using losartan as a substrate, and determine the role of Q214 side chain in the binding of the drug substrates near the heme in the active site using computational docking. A total of 5 important substrates (Glyburide, Irbesartan, Tolbutamide, S-warfarin, and Losartan) of CYP2C9 were studied via molecular docking to characterize the effect of variation to threonine at 477 on drug metabolism. The data suggest that the reorientation of Q214 side chain to interact with T477 in CYP2C9*30 may affect binding of drugs near the heme in the active site, which can lead to altered metabolism profile compared to the wild-type. Overall, the results yield insights into the reduced catalytic activity of the CYP2C9*30 and may help understand the differences in drug metabolism.
Download or read book Cytochromes written by David F.V. Lewis and published by Taylor & Francis. This book was released on 2003-09 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Study of Bacteriorhodopsin in a Controlled Lipid Environment written by Vivien Yeh and published by Springer. This book was released on 2018-10-26 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Grants Index written by National Institutes of Health (U.S.). Division of Research Grants and published by . This book was released on 1975 with total page 1224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Vibrational Spectroscopy in Life Science written by Friedrich Siebert and published by John Wiley & Sons. This book was released on 2008-07-15 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors describe basic theoretical concepts of vibrational spectroscopy, address instrumental aspects and experimental procedures, and discuss experimental and theoretical methods for interpreting vibrational spectra. It is shown how vibrational spectroscopy provides information on general aspects of proteins, such as structure, dynamics, and protein folding. In addition, the authors use selected examples to demonstrate the application of Raman and IR spectroscopy to specific biological systems, such as metalloproteins, and photoreceptors. Throughout, references to extensive mathematical and physical aspects, involved biochemical features, and aspects of molecular biology are set in boxes for easier reading. Ideal for undergraduate as well as graduate students of biology, biochemistry, chemistry, and physics looking for a compact introduction to this field.
Download or read book Analytical Techniques for the Elucidation of Protein Function written by Isao Suetake and published by John Wiley & Sons. This book was released on 2023-03-27 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: ANALYTICAL TECHNIQUES FOR THE ELUCIDATION OF PROTEIN FUNCTION An essential aid for scientists seeking alternative techniques for investigating proteins Proteins are the building blocks of living organisms, and they play an enormous range of fundamental roles in sustaining and shaping life. The critical determinant of a protein’s function is its structure, and the analysis of protein structures has therefore become a significant component of biological research. In recent years, longstanding analytical techniques such as X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy have been supplemented by a number of new methods which promise to revolutionize the study of proteins and their functions. Analytical Techniques for the Elucidation of Protein Function serves as an introduction to these techniques, which are especially crucial for analyzing intrinsically disordered regions and post-translational modifications. These have revolutionized the study of proteins in recent years, and conventional methods for analyzing protein structures are no longer sufficient to work through their ramifications. This book therefore brings greater awareness of techniques which promise to produce the very cutting edge of protein research. Analytical Techniques for the Elucidation of Protein Function readers will find: A discussion of techniques including electron paramagnetic resonance (ESR) spectroscopy, neutron scattering, Raman imaging, and more Both theoretical background and practical applications for each technique Contributions from leading international researchers into protein structure and function This practically focused text is a valuable reference for protein and peptide analysis and synthesis researchers, as well as for graduate and advanced undergraduate students in the life sciences.
