Download or read book Quantitative Biology Dynamics of Living Systems written by Noriko Hiroi and published by Frontiers Media SA. This book was released on 2017-07-24 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the emergence of Systems Biology, there is a greater realization that the whole behavior of a living system may not be simply described as the sum of its elements. To represent a living system using mathematical principles, practical quantities with units are required. Quantities are not only the bridge between mathematical description and biological observations; they often stand as essential elements similar to genome information in genetics. This important realization has greatly rejuvenated research in the area of Quantitative Biology. Because of the increased need for precise quantification, a new era of technological development has opened. For example, spatio-temporal high-resolution imaging enables us to track single molecule behavior in vivo. Clever artificial control of experimental conditions and molecular structures has expanded the variety of quantities that can be directly measured. In addition, improved computational power and novel algorithms for analyzing theoretical models have made it possible to investigate complex biological phenomena. This research topic is organized on two aspects of technological advances which are the backbone of Quantitative Biology: (i) visualization of biomolecules, their dynamics and function, and (ii) generic technologies of model optimization and numeric integration. We have also included articles highlighting the need for new quantitative approaches to solve some of the long-standing cell biology questions. In the first section on visualizing biomolecules, four cutting-edge techniques are presented. Ichimura et al. provide a review of quantum dots including their basic characteristics and their applications (for example, single particle tracking). Horisawa discusses a quick and stable labeling technique using click chemistry with distinct advantages compared to fluorescent protein tags. The relatively small physical size, stability of covalent bond and simple metabolic labeling procedures in living cells provides this type of technology a potential to allow long-term imaging with least interference to protein function. Obien et al. review strategies to control microelectrodes for detecting neuronal activity and discuss techniques for higher resolution and quality of recordings using monolithic integration with on-chip circuitry. Finally, the original research article by Amariei et al. describes the oscillatory behavior of metabolites in bacteria. They describe a new method to visualize the periodic dynamics of metabolites in large scale cultures populations. These four articles contribute to the development of quantitative methods visualizing diverse targets: proteins, electrical signals and metabolites. In the second section of the topic, we have included articles on the development of computational tools to fully harness the potential of quantitative measurements through either calculation based on specific model or validation of the model itself. Kimura et al. introduce optimization procedures to search for parameters in a quantitative model that can reproduce experimental data. They present four examples: transcriptional regulation, bacterial chemotaxis, morphogenesis of tissues and organs, and cell cycle regulation. The original research article by Sumiyoshi et al. presents a general methodology to accelerate stochastic simulation efforts. They introduce a method to achieve 130 times faster computation of stochastic models by applying GPGPU. The strength of such accelerated numerical calculation are sometimes underestimated in biology; faster simulation enables multiple runs and in turn improved accuracy of numerical calculation which may change the final conclusion of modeling study. This also highlights the need to carefully assess simulation results and estimations using computational tools.

Download or read book Microscopic Image Analysis for Life Science Applications written by Jens Rittscher and published by Artech House. This book was released on 2008 with total page 533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here's a first-of-its-kind book that bridges the gap between biomedical imaging and the bioscience community. This unique resource gives you a detailed understanding of imaging platforms, fluorescence imaging, and fundamental image processing algorithms. Further, it guides you through application of advanced image analysis methods and techniques to specific biological problems. The book presents applications that span a wide range of scales, from the detection of signaling events in sub-cellular structures, to the automated analysis of tissue structures. Other critical areas discussed include the dynamics of cell populations and in vivo microscopy. This cutting-edge volume is supported with over 160 illustrations that support key topics throughout the book. CD-ROM Included! Contains full-color images and videos that further illustrate topics discussed in the book.

Download or read book Imaging Cellular and Molecular Biological Functions written by Spencer L. Shorte and published by Springer Science & Business Media. This book was released on 2007-09-12 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Download or read book Understanding Light Microscopy written by Jeremy Sanderson and published by John Wiley & Sons. This book was released on 2019-03-04 with total page 851 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Download or read book Microscope Image Processing written by Fatima Merchant and published by Academic Press. This book was released on 2022-08-26 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microscope Image Processing, Second Edition, introduces the basic fundamentals of image formation in microscopy including the importance of image digitization and display, which are key to quality visualization. Image processing and analysis are discussed in detail to provide readers with the tools necessary to improve the visual quality of images, and to extract quantitative information. Basic techniques such as image enhancement, filtering, segmentation, object measurement, and pattern recognition cover concepts integral to image processing. In addition, chapters on specific modern microscopy techniques such as fluorescence imaging, multispectral imaging, three-dimensional imaging and time-lapse imaging, introduce these key areas with emphasis on the differences among the various techniques.The new edition discusses recent developments in microscopy such as light sheet microscopy, digital microscopy, whole slide imaging, and the use of deep learning techniques for image segmentation and analysis with big data image informatics and management.Microscope Image Processing, Second Edition, is suitable for engineers, scientists, clinicians, post-graduate fellows and graduate students working in bioengineering, biomedical engineering, biology, medicine, chemistry, pharmacology and related fields, who use microscopes in their work and would like to understand the methodologies and capabilities of the latest digital image processing techniques or desire to develop their own image processing algorithms and software for specific applications. - Presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms - Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments - Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Download or read book Handbook of Imaging in Biological Mechanics written by Corey P. Neu and published by CRC Press. This book was released on 2014-10-24 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Emerging imaging techniques have opened new fronts to investigate tissues, cells, and proteins. Transformative technologies such as microCT scans, super-resolution microscopy, fluorescence-based tools, and other methods now allow us to study the mechanics of cancer, dissect the origins of cellular force regulation, and examine biological specimens

Download or read book Microscope Image Processing written by Qiang Wu and published by Elsevier. This book was released on 2010-07-27 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Digital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology. Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and display, object measurement and classification, autofocusing, and structured illumination. Key Features: - Detailed descriptions of many leading-edge methods and algorithms - In-depth analysis of the method and experimental results, taken from real-life examples - Emphasis on computational and algorithmic aspects of microscope image processing - Advanced material on geometric, morphological, and wavelet image processing, fluorescence, three-dimensional and time-lapse microscopy, microscope image enhancement, MultiSpectral imaging, and image data management This book is of interest to all scientists, engineers, clinicians, post-graduate fellows, and graduate students working in the fields of biology, medicine, chemistry, pharmacology, and other related fields. Anyone who uses microscopes in their work and needs to understand the methodologies and capabilities of the latest digital image processing techniques will find this book invaluable. - Presents a unique practical perspective of state-of-the-art microcope image processing and the development of specialized algorithms - Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments - Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Download or read book Imaging and Spectroscopic Analysis of Living Cells written by and published by Academic Press. This book was released on 2012-01-25 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods

Download or read book Computational Systems Bioinformatics Proceedings Of The Conference Csb 2006 written by Peter Markstein and published by World Scientific. This book was released on 2006-07-28 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains about 40 papers covering many of the latest developments in the fast-growing field of bioinformatics. The contributions span a wide range of topics, including computational genomics and genetics, protein function and computational proteomics, the transcriptome, structural bioinformatics, microarray data analysis, motif identification, biological pathways and systems, and biomedical applications. There are also abstracts from the keynote addresses and invited talks.The papers cover not only theoretical aspects of bioinformatics but also delve into the application of new methods, with input from computation, engineering and biology disciplines. This multidisciplinary approach to bioinformatics gives these proceedings a unique viewpoint of the field./a

Download or read book Computational Systems Bioinformatics written by Peter Markstein and published by Imperial College Press. This book was released on 2006 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains about 40 papers covering many of the latest developments in the fast-growing field of bioinformatics. The contributions span a wide range of topics, including computational genomics and genetics, protein function and computational proteomics, the transcriptome, structural bioinformatics, microarray data analysis, motif identification, biological pathways and systems, and biomedical applications. There are also abstracts from the keynote addresses and invited talks.The papers cover not only theoretical aspects of bioinformatics but also delve into the application of new methods, with input from computation, engineering and biology disciplines. This multidisciplinary approach to bioinformatics gives these proceedings a unique viewpoint of the field.

Download or read book Fluorescent Methods to Study Biological Membranes written by Yves Mely and published by Springer Science & Business Media. This book was released on 2012-10-10 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Download or read book Single Biomolecule Detection and Analysis written by Tuhin Subhra Santra and published by CRC Press. This book was released on 2023-08-15 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This collection discusses various micro/nanodevice design and fabrication for single-biomolecules detection. It will be an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. This book- Discusses techniques of single-biomolecule detection, their advantages, limitations, and applications. Covers comprehensively several electrochemical detection techniques. Provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail. Examines different types of cantilever-based biomolecule sensing, and its limitations. Single Biomolecule Detection and Analysis covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices, electrical and magnetic detection technologies, microscopy and spectroscopy techniques, single biomolecule optical, and nanopore devices. The text covers key important biosensors-based detection, stochastic optical reconstruction microscopy-based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and Fluorescence Correlation Spectroscopy (FCS) detection. The text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. Discussing chemical process, physical process, separation, sensing, imaging, sequencing, and analysis of single-molecule detection, this text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers microscopy and spectroscopy techniques for single-biomolecule detection, analysis, and their biomedical engineering applications.

Download or read book Encyclopedia of Cell Biology written by and published by Academic Press. This book was released on 2015-08-07 with total page 2972 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of Cell Biology, Four Volume Set offers a broad overview of cell biology, offering reputable, foundational content for researchers and students across the biological and medical sciences. This important work includes 285 articles from domain experts covering every aspect of cell biology, with fully annotated figures, abundant illustrations, videos, and references for further reading. Each entry is built with a layered approach to the content, providing basic information for those new to the area and more detailed material for the more experienced researcher. With authored contributions by experts in the field, the Encyclopedia of Cell Biology provides a fully cross-referenced, one-stop resource for students, researchers, and teaching faculty across the biological and medical sciences. Fully annotated color images and videos for full comprehension of concepts, with layered content for readers from different levels of experience Includes information on cytokinesis, cell biology, cell mechanics, cytoskeleton dynamics, stem cells, prokaryotic cell biology, RNA biology, aging, cell growth, cell Injury, and more In-depth linking to Academic Press/Elsevier content and additional links to outside websites and resources for further reading A one-stop resource for students, researchers, and teaching faculty across the biological and medical sciences

Download or read book Visualizing RNA Dynamics in the Cell written by and published by Academic Press. This book was released on 2016-05-27 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in visualizing RNA dynamics in the cell - Contains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe

Download or read book Center for Nanoscale Science and Technology 2010 Biennial Report written by and published by DIANE Publishing. This book was released on with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Giant Vesicle Book written by Rumiana Dimova and published by CRC Press. This book was released on 2019-11-19 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features • A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications • A start-up package of theoretical and experimental information for newcomers in the field • Extensive protocols for establishing the required preparations and assays • Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls • Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques • Handy tables with data and structures for ready reference

Download or read book The Journal of Cell Biology written by and published by . This book was released on 2008 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-