Download or read book Flagellar Motors and Force Sensing in Bacteria written by Matt Arthur Baker and published by Frontiers Media SA. This book was released on 2022-02-28 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Bacterial Flagellum written by Tohru Minamino and published by Humana. This book was released on 2018-06-21 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Download or read book Perspectives on Bacterial Flagellar Motor written by Tohru Minamino and published by . This book was released on 2021-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: The bacterial flagellum is a supramolecular motility machinery consisting of the basal body acting as a rotary motor, the hook as a universal joint and the filament as a helical propeller. The bacterial flagellar motor composed of a rotor ring and a dozen stators is powered by an electrochemical-potential difference of specific ions across the cytoplasmic membrane and rotates in either the counterclockwise (CCW) or clockwise (CW) direction. A sensory signal transduction pathway regulates the switching between the CCW and CW states of the motor in response to environmental stimuli, allowing bacterial cells to migrate more desirable environments for their survival. The core structure of the bacterial flagellum is conserved among bacterial species. However, recent structural analyses of intact flagellar structures derived from various bacterial species by electron cryotomography and subtomogram averaging have shown that novel and divergent structures surround the core structure, suggesting that the flagellar motors have adapted to function in various environments of the habitat of bacteria. This Special Issue of Biomolecules covers recent advances in our understanding of and perspectives on the flagellar motor derived from different bacterial species.
Download or read book Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria 2 Volume Set written by Frans J. de Bruijn and published by John Wiley & Sons. This book was released on 2016-09-06 with total page 1460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria in various habitats are subject to continuously changing environmental conditions, such as nutrient deprivation, heat and cold stress, UV radiation, oxidative stress, dessication, acid stress, nitrosative stress, cell envelope stress, heavy metal exposure, osmotic stress, and others. In order to survive, they have to respond to these conditions by adapting their physiology through sometimes drastic changes in gene expression. In addition they may adapt by changing their morphology, forming biofilms, fruiting bodies or spores, filaments, Viable But Not Culturable (VBNC) cells or moving away from stress compounds via chemotaxis. Changes in gene expression constitute the main component of the bacterial response to stress and environmental changes, and involve a myriad of different mechanisms, including (alternative) sigma factors, bi- or tri-component regulatory systems, small non-coding RNA’s, chaperones, CHRIS-Cas systems, DNA repair, toxin-antitoxin systems, the stringent response, efflux pumps, alarmones, and modulation of the cell envelope or membranes, to name a few. Many regulatory elements are conserved in different bacteria; however there are endless variations on the theme and novel elements of gene regulation in bacteria inhabiting particular environments are constantly being discovered. Especially in (pathogenic) bacteria colonizing the human body a plethora of bacterial responses to innate stresses such as pH, reactive nitrogen and oxygen species and antibiotic stress are being described. An attempt is made to not only cover model systems but give a broad overview of the stress-responsive regulatory systems in a variety of bacteria, including medically important bacteria, where elucidation of certain aspects of these systems could lead to treatment strategies of the pathogens. Many of the regulatory systems being uncovered are specific, but there is also considerable “cross-talk” between different circuits. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria is a comprehensive two-volume work bringing together both review and original research articles on key topics in stress and environmental control of gene expression in bacteria. Volume One contains key overview chapters, as well as content on one/two/three component regulatory systems and stress responses, sigma factors and stress responses, small non-coding RNAs and stress responses, toxin-antitoxin systems and stress responses, stringent response to stress, responses to UV irradiation, SOS and double stranded systems repair systems and stress, adaptation to both oxidative and osmotic stress, and desiccation tolerance and drought stress. Volume Two covers heat shock responses, chaperonins and stress, cold shock responses, adaptation to acid stress, nitrosative stress, and envelope stress, as well as iron homeostasis, metal resistance, quorum sensing, chemotaxis and biofilm formation, and viable but not culturable (VBNC) cells. Covering the full breadth of current stress and environmental control of gene expression studies and expanding it towards future advances in the field, these two volumes are a one-stop reference for (non) medical molecular geneticists interested in gene regulation under stress.
Download or read book Physical Microbiology written by Guillaume Duménil and published by Springer Nature. This book was released on 2020-09-07 with total page 141 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book emerges from the idea that specific physics-inspired approaches are necessary to understand different stage of bacterial physiology and the infections they cause. Many aspects of bacterial life depend on processes typically described by physical laws: The rheology of biofilms is determined by complex cohesive forces. Physical laws of diffusion are essential to all processes of bacterial metabolism. The formation of the numerous bacterial biomacromolecules require complex self-organization processes and their function are powered by potent molecular motors. Host-pathogen interactions during infection frequently occur in environments determined by fluid mechanics. In this book, different chapters represent research at the interface between microbiology and physics. Topics range from intracellular organization to cell-cell interactions. A good part of the book is devoted to mechanical forces, which are involved in the function of elaborate bacterial nanomachines, chromosome segregation, and cell division. The effect of bacterial toxins provides an example of the alteration of cellular membrane properties by bacteria. Symmetrically, histones from mammalian cells alter bacterial membranes as a defense mechanism during infection. The editors of this book, Guillaume Duménil and Sven van Teeffelen, have selected researchers at the forefront of research in physical microbiology to provide the most recent view in this fast-moving field. The contents of this book are designed to be accessible for scientists with training in biology and for scientists with training in physics. The objective is to provide a fresh perspective on microbiology and infection by highlighting recent multidisciplinary research and favor rapid advances at this fruitful interface.
Download or read book Cellular Imaging written by Eric Hanssen and published by Springer. This book was released on 2017-12-30 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Download or read book E coli in Motion written by Howard C. Berg and published by Springer Science & Business Media. This book was released on 2008-01-11 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Escherichia coli, commonly referred to as E. coli, has been the organism of choice for molecular genetics for decades. Its machinery and mobile behavior is one of the most fascinating topics for cell scientists. Scientists and engineers, not trained in microbiology, and who would like to learn more about living machines, can see it as a unique example. This cross-disciplinary monograph covers more than thirty years of research and is accessible to graduate students and scientists alike.
Download or read book Bacterial Signaling written by Reinhard Krämer and published by John Wiley & Sons. This book was released on 2009-12-09 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive insight into cellular signaling processes in bacteria with a special focus on biotechnological implications, this is the first book to cover intercellular as well as intracellular signaling and its relevance for biofilm formation, host pathogen interactions, symbiotic relationships, and photo- and chemotaxis. In addition, it deals in detail with principal bacterial signaling mechanisms -- making this a valuable resource for all advanced students in microbiology. Dr. Krämer is a world-renowned expert in intracellular signaling and its implications for biotechnology processes, while Dr. Jung is an expert on intercellular signaling and its relevance for biomedicine and agriculture.
Download or read book Bioenergetics 2 written by David G. Nicholls and published by Academic Press. This book was released on 2014-06-28 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioenergetics 2 aims to clarify topics such as the thermodynamics of bioenergetic processes and the stoichiometries of energy coupling reactions. The book discusses chemiosmotic energy transduction; ion transport across energy-conserving membranes; and quantitative bioenergenetics as the measurement of driving forces. The text also describes the chemiosmotic proton circuit; the respiratory chain; the photosynthetic generators of protonmotive force; and the ATP synthase. The secondary transport of products across the membrane, as well as the structures of the bacterial photosynthetic reaction center and bacteriorhodopsin are also considered. Biochemists will find the book invaluable.
Download or read book Biological Rotary Nanomotors written by Michael Manson and published by Frontiers Media SA. This book was released on 2023-02-09 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Salmonella written by Maria Teresa Mascellino and published by BoD – Books on Demand. This book was released on 2018-07-18 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the microorganism Salmonella. This bacterium is well known for a long time, being involved in systemic (typhus and paratyphus infections) and nonsystemic diseases such as food poisoning. Major and minor Salmonellae are widespread worldwide in developing countries and industrialized areas, respectively. In 2015, about 3576 Salmonella strains have been isolated from human infections in Italy. S. typhimurium and S. enteritidis are the most prevalent serotypes and represent 80% of cases of infections over the last 10 years. The antibiotic susceptibility decrease over the last decades is a big issue in the management of this bacterium, once considered easy to treat. The use of antibiotic combinations in order to overcome the microorganism resistance should be hoped.
Download or read book Macromolecular Protein Complexes III Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-11-30 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers important topics such as the dynamic structure and function of the 26S proteasome, the DNA replication machine: structure and dynamic function and the structural organization and protein–protein interactions in the human adenovirus capsid, to mention but a few. The 18 chapters included here, written by experts in their specific field, are at the forefront of scientific knowledge. The impressive integration of structural data from X-ray crystallography with that from cryo-electron microscopy is apparent throughout the book. In addition, functional aspects are also given a high priority. Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Bacterial and Archaeal Motility written by Tohru Minamino and published by Springer Nature. This book was released on 2023-02-26 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Bacterial and Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.
Download or read book Implication of Quorum Sensing System in Biofilm Formation and Virulence written by Pallaval Veera Bramhachari and published by Springer. This book was released on 2019-01-28 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the importance and significance of Quorum sensing (QS), it’s critical roles in regulating diverse cellular functions in microbes, including bioluminescence, virulence, pathogenesis, gene expression, biofilm formation and antibiotic resistance. Microbes can coordinate population behavior with small molecules called autoinducers (AHL) which serves as a signal of cellular population density, triggering new patterns of gene expression for mounting virulence and pathogenesis. Therefore, these microbes have the competence to coordinate and regulate explicit sets of genes by sensing and communicating amongst themselves utilizing variety of signals. This book descry emphasizes on how bacteria can coordinate an activity and synchronize their response to external signals and regulate gene expression. The chapters of the book provide the recent advancements on various functional aspects of QS systems in different gram positive and gram negative organisms. Finally, the book also elucidates a comprehensive yet a representative description of a large number of challenges associated with quorum sensing signal molecules viz. virulence, pathogenesis, antibiotic synthesis, biosurfactants production, persister cells, cell signaling and biofilms, intra and inter-species communications, host-pathogen interactions, social interactions & swarming migration in biofilms.
Download or read book Micro Nanorobotic Manipulation Systems and Their Applications written by Toshio Fukuda and published by Springer Science & Business Media. This book was released on 2013-02-28 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro/Nano Robotics and Automation technologies have rapidly grown associated with the growth of Micro and Nanotechnologies. This book presents a summary of fundamentals in micro-nano scale engineering and the current state of the art of these technologies. “Micro-Nanorobotic Manipulation Systems and their Applications” introduces these advanced technologies from the basics and applications aspects of Micro/Nano-Robotics and Automation from the prospective micro/nano-scale manipulation. The book is organized in 9 chapters including an overview chapter of Micro/Nanorobotics and Automation technology from the historical view and important related research works. Further chapters are devoted to the physics of micro-nano fields as well as to material and science, microscopes, fabrication technology, importance of biological cell, and control techniques. Furthermore important examples, applications and a concise summary of Micro-Nanorobotics and Automation technologies are given.
Download or read book General Microbiology written by Linda Bruslind and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Welcome to the wonderful world of microbiology! Yay! So. What is microbiology? If we break the word down it translates to "the study of small life," where the small life refers to microorganisms or microbes. But who are the microbes? And how small are they? Generally microbes can be divided in to two categories: the cellular microbes (or organisms) and the acellular microbes (or agents). In the cellular camp we have the bacteria, the archaea, the fungi, and the protists (a bit of a grab bag composed of algae, protozoa, slime molds, and water molds). Cellular microbes can be either unicellular, where one cell is the entire organism, or multicellular, where hundreds, thousands or even billions of cells can make up the entire organism. In the acellular camp we have the viruses and other infectious agents, such as prions and viroids. In this textbook the focus will be on the bacteria and archaea (traditionally known as the "prokaryotes,") and the viruses and other acellular agents.
Download or read book Research at the Intersection of the Physical and Life Sciences written by National Research Council and published by National Academies Press. This book was released on 2010-03-25 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: Traditionally, the natural sciences have been divided into two branches: the biological sciences and the physical sciences. Today, an increasing number of scientists are addressing problems lying at the intersection of the two. These problems are most often biological in nature, but examining them through the lens of the physical sciences can yield exciting results and opportunities. For example, one area producing effective cross-discipline research opportunities centers on the dynamics of systems. Equilibrium, multistability, and stochastic behavior-concepts familiar to physicists and chemists-are now being used to tackle issues associated with living systems such as adaptation, feedback, and emergent behavior. Research at the Intersection of the Physical and Life Sciences discusses how some of the most important scientific and societal challenges can be addressed, at least in part, by collaborative research that lies at the intersection of traditional disciplines, including biology, chemistry, and physics. This book describes how some of the mysteries of the biological world are being addressed using tools and techniques developed in the physical sciences, and identifies five areas of potentially transformative research. Work in these areas would have significant impact in both research and society at large by expanding our understanding of the physical world and by revealing new opportunities for advancing public health, technology, and stewardship of the environment. This book recommends several ways to accelerate such cross-discipline research. Many of these recommendations are directed toward those administering the faculties and resources of our great research institutions-and the stewards of our research funders, making this book an excellent resource for academic and research institutions, scientists, universities, and federal and private funding agencies.
