Download or read book Fish Chromosome Methodology written by Thomas E. Denton and published by Charles C. Thomas Publisher. This book was released on 1973 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fish Cytogenetic Techniques written by Catherine Ozouf-Costaz and published by CRC Press. This book was released on 2015-06-17 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chro
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Methods for Fish Biology written by Carl B. Schreck and published by . This book was released on 1990 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed as both a reference and a handbook for the study of fishes. It is a source of methods commonly used to research fish genetics, systematics, anatomy, physiology, developmental biology, toxicology, behavior, and ecology. Standard methods and their theoretical framework are presented for all these fields. Each of the book's 20 chapters also contains a background literature review which, though not exhaustive, allows readers to delve more deeply into subjects that particularly interest them. The main emphasis is on methodology, but the pros and cons of alternative procedures also are treated, as are the uses and misuses of data generated by the techniques.
Download or read book Fish Karyotypes written by Ryoichi Arai and published by Springer Science & Business Media. This book was released on 2011-06-26 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the largest group of extant vertebrates, fish offer an almost limitless number of striking examples of evolutionary adaptation to environmental and biotic selection pressure. The most diverse of all vertebrate groups, the higher taxa of fish traditionally have been classified by morphology and paleontology, with a much smaller input of cytogenetic information. DNA sequence data are exerting an increasingly strong influence on modern fish systematics, challenging the classification of numerous higher taxa ranging from genera to orders. The most fruitful approach, however, involves synthetic analyses of morphology, molecular phylogenetics, comparative karyology, and genome size. Karyotypes of more than 3400 species/subspecies are arranged here by fish systematics and include a list of genome size, sex chromosomes, B chromosomes, polyploidy, and locality of material fish, among others. This volume enables both beginners and advanced researchers to survey the existing literature and facilitates the implementation of an integrative approach to fish systematics. The first book on fish chromosomes in nearly 15 years, it is also the most comprehensive.
Download or read book FISH Technology written by Bernd W. Rautenstrauß and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Download or read book Chromosome Analysis written by Eisuke Gotoh and published by Springer Nature. This book was released on 2022-09-06 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Download or read book Molecular Cytogenetics of Fishes written by Marcelo B. Cioffi and published by S. Karger AG (Switzerland). This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fish exhibit the greatest diversity of all vertebrates, making this group extremely attractive for the study of a number of evolutionary questions. Besides, fish genomes have intrinsic characteristics that may be responsible for this amazing diversity, but little is known about their structure and organization. Major advances in chromosomal studies were achieved in the current 'molecular cytogenetic era' based on detecting DNA sequences on chromosomes, parts of chromosomes or even whole genomic DNA by fluorescence in situ hybridization. In this issue, a collection of review articles is presented together with current research results, demonstrating the advances made using this specific methodology in fish research. The contributions provide a timely update on recent developments in fish molecular cytogenetics, covering topics such as karyotype evolution, sex and B chromosomes, polyploids, and molecular evolution. Researchers, teachers, and students interested in the structural features and evolutionary dynamics of fish genomes will find this publication a valuable source of current knowledge.
Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Download or read book Fish Cytogenetics written by E Pisano and published by CRC Press. This book was released on 2007-01-03 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past 20 years, fish cytogenetics has become an essential tool in fields as diverse as systematics and evolution, conservation, aquaculture and more recently, genomics. This book is organized in four sections (systematics and evolution; biodiversity conservation; stock assessment and aquaculture; genomics) covering the major fields of present
Download or read book Sex Control in Aquaculture written by Hanping Wang and published by John Wiley & Sons. This book was released on 2018-11-08 with total page 1810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Awarded Bookauthority's "Best Aquaculture Books of all Time" A comprehensive resource that covers all the aspects of sex control in aquaculture written by internationally-acclaimed scientists Comprehensive in scope, Sex Control in Aquaculture first explains the concepts and rationale for sex control in aquaculture, which serves different purposes. The most important are: to produce monosex stocks to rear only the fastest-growing sex in some species, to prevent precocious or uncontrolled reproduction in other species and to aid in broodstock management. The application of sex ratio manipulation for population control and invasive species management is also included. Next, this book provides detailed and updated information on the underlying genetic, epigenetic, endocrine and environmental mechanisms responsible for the establishment of the sexes, and explains chromosome set manipulation techniques, hybridization and the latest gene knockout approaches. Furthermore, the book offers detailed protocols and key summarizing information on how sex control is practiced worldwide in 35 major aquaculture species or groups, including fish and crustaceans, and puts the focus on its application in the aquaculture industry. With contributions from an international panel of leading scientists, Sex Control in Aquaculture will appeal to a large audience: aquaculture/fisheries professionals and students, scientists or biologists working with basic aspects of fish/shrimp biology, growth and reproductive endocrinology, genetics, molecular biology, evolutionary biology, and R&D managers and administrators. This text explores sex control technologies and monosex production of commercially-farmed fish and crustacean species that are highly in demand for aquaculture, to improve feed utilization efficiency, reduce energy consumption for reproduction and eliminate a series of problems caused by mixed sex rearing. Thus, this book: Contains contributions from an international panel of leading scientists and professionals in the field Provides comprehensive coverage of both established and new technologies to control sex ratios that are becoming more necessary to increase productivity in aquaculture Includes detailed coverage of the most effective sex control techniques used in the world's most important commercially-farmed species Sex Control in Aquaculture is the comprehensive resource for understanding the biological rationale, scientific principles and real-world practices in this exciting and expanding field.
Download or read book Cytogenetics written by Marcelo L. Larramendy and published by BoD – Books on Demand. This book was released on 2019-05-10 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics - Past, Present, and Further Perspectives discusses events that influenced the development of cytogenetics as a specialty within biology, with special attention paid to methodological achievements developed worldwide that have driven the field forward. Improvements to the resolution of chromosome analysis followed closely the introduction of innovative analytical technologies. In that sense, this book reviews and provides a brief account of the structure of chromosomes and stresses the high structural conservation in different species with an emphasis on aspects that require further research. However, it should be kept in mind that the future of cytogenetics will likely depend on improved knowledge of chromosome structure and function.
Download or read book Chromosome Techniques written by Arun Sharma and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.
Download or read book Multicolor FISH in Human Cytogenetics written by Thomas Liehr and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This special issue focuses on the recent developments in the exciting and continuously progressing field of human multicolor fluorescence in situ hybridisation (mFISH), highlighting the advantages, applications and possible limitations of this technique. Today, mFISH assays are indispensable for the precise characterization of complex chromosome rearrangements and numerical chromosome aberrations. In a first, more technically oriented part, the main mFISH-techniques (M-FISH, SKY, COBRA) are reviewed and their applications described. In the second part, world experts in the field discuss the multitude of presently available techniques and present their latest findings based on the use of mFISH in tumor cytogenetics, clinical genetics and basic investigations. The most recent studies employing mFISH in the diagnosis of leukemia, lymphoma and solid tumors are included as are preimplantation, pre- and postnatal clinical genetic studies using interphase-mFISH probe sets, FISH-banding and DNA-array techniques. Since structurally rearranged marker chromosomes have implications for hereditary and acquired diseases, chromosomal breakpoints are characterized and evaluated. This unique, comprehensive and up-to-date volume will be a valuable resource for (molecular) cytogeneticists, genetic counsellors, cell biologists, geneticists, reproductive scientists, obstetricians, pediatricians, oncologists and hematologists.
Download or read book Plant Chromosomes written by Kiichi Fukui and published by CRC Press. This book was released on 2017-12-14 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.
Download or read book Chromosome Techniques written by Archarna Sharma and published by CRC Press. This book was released on 2020-08-18 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.
Download or read book Diagnostic Cytogenetics written by Rolf-Dieter Wegner and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following a section on tissue culture, chromosome staining and basic information about karyotyping, this text presents nomenclature and quality standards, as well as protocols of relevance to comprehensive cytogenetic diagnostics.
