Download or read book Fingerprinting Methods Based on Arbitrarily Primed PCR written by Maria R. Micheli and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes. The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.
Download or read book Bacterial Genomes written by F.J. de Bruijn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide range of microbiologists, molecular biologists, and molecular evolutionary biologists will find this new volume of singular interest. It summarizes the present knowledge about the structure and stability of microbial genomes, and reviews the techniques used to analyze and fingerprint them. Maps of approximately thirty important microbes, along with articles on the construction and relevant features of the maps are included. The volume is not intended as a complete compendium of all information on microbial genomes, but rather focuses on approaches, methods and good examples of the analysis of small genomes.
Download or read book PCR in Bioanalysis written by Stephen J Meltzer and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Download or read book DNA Fingerprinting in Plants written by Kurt Weising and published by CRC Press. This book was released on 2005-02-28 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p
Download or read book Analytical Molecular Biology written by Helen Parkes and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement) Programme, introduces the issues of validation and quality to the bioanalytical community, specifically addressing DNA-based analyses. It aims to raise awareness of the factors that can influence the validity of DNA analysis and the production of quality data. Emphasis is placed on VAM principles, as well as additional challenges that are associated with the analysis of real samples, for example, complex food matrices or forensic samples that have been subjected to environmental insult. Information is collated from a variety of sources including literature, discussions and LGC research, and offers constructive advice where possible.
Download or read book Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA written by Graham R. Taylor and published by CRC Press. This book was released on 1997-01-24 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Download or read book Molecular Microbial Ecology written by Mark Osborn and published by Garland Science. This book was released on 2004-03-01 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.
Download or read book PCR written by Lucília Domingues and published by Springer Nature. This book was released on 2023-09-23 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Download or read book Plant Genotyping written by Robert J. Henry and published by CABI. This book was released on 2001-08-17 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant genotyping, or DNA fingerprinting of plants, is a technology that has matured and is poised for widespread practical application in the fields of breeding, commerce and research. This book examines the technologies available and their application in the analysis of:Wild plant populationsGermplasm collections Plant breedingContributors include leading research workers in this field from North America, Europe and Australasia.
Download or read book Wood Formation in Trees written by Nigel J Chaffey and published by CRC Press. This book was released on 2002-01-10 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt: Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of
Download or read book The Polymerase Chain Reaction written by Kary B. Mullis and published by Springer Science & Business Media. This book was released on 2012-02-02 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Download or read book DNA Based Markers in Plants written by R.L. Phillips and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the new techniques described in this volume, a new gene can be placed on the linkage map within only a few days. Leading researchers have updated the earlier edition to include the latest versions of DNA-based marker maps for a variety of important crops.
Download or read book Manual of Commercial Methods in Clinical Microbiology written by Allan L. Truant and published by American Society for Microbiology Press. This book was released on 2001-12-18 with total page 1557 pages. Available in PDF, EPUB and Kindle. Book excerpt: A general resource for all subdisciplines of clinical microbiology to use when evaluating commercial methods, tests, or procedures. • Reviews all the commercially available tests (both manual and automated) in the discipline of clinical microbiology. • Includes a description of the sensitivities, specificities, and predictive values from peer-reviewed sources. • Features separate chapters devoted to molecular microbiology, information management, emerging infectious diseases, and veterinary clinical microbiology.
Download or read book DNA Methods in Clinical Microbiology written by P. Singleton and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology. The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens. Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.
Download or read book Polymerase Chain Reaction written by Patricia Hernandez-Rodriguez and published by BoD – Books on Demand. This book was released on 2012-05-30 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.
Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Download or read book DNA Methylation written by Manel Esteller and published by CRC Press. This book was released on 2004-09-29 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Methylation: Approaches, Methods and Applications describes the relation DNA methylation has to gene silencing in disease, and explores its promising role in treating cancer. Written by leaders in the field, this exceptional compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation
