Download or read book Fabrication and Characterization of a Microfluidic Device for 3D Cells Analysis written by Simone Benetto and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Fabrication and Characterization of a Microfluidic Device for Ultrasound based Cell Sorting written by Pratik Anirudha Ambekar and published by . This book was released on 2021 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: The isolation and sorting of cells is an important process in research and hospital laboratories. Most research laboratories incorporate fluorescently or magnetically labeled antibodies adherent to cell surface antigens for cell identification and separation. MiCS (Microbubble Cell Sorting) is a new technique that overcomes the limitations of existing cell sorting techniques by using ultrasound traveling waves and microbubble tags. A microfluidic device consisting of a flow channel was developed for effectively performing MiCS. The device was designed with the aim of obtaining traveling waves in the flow channel by using various design considerations and Finite Element Analysis (FEA) simulations. Placing the flow channel in the acoustic far-field provided the necessary traveling-wave regime. Subsequently, the device was fabricated by employing manufacturing processes like laser cutting, micromachining, and acrylic bonding. The device was then characterized by performing electrical impedance analysis, flow continuity experiments, and pulse-echo testing. Results suggested that flow in the flow channel was continuous, and the transducer operated effectively.
Download or read book 3D Printed Microfluidic Devices written by Savas Tasoglu and published by MDPI. This book was released on 2019-01-10 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "3D Printed Microfluidic Devices" that was published in Micromachines
Download or read book Microfluidics for Cells and Other Organisms written by Danny van Noort and published by MDPI. This book was released on 2019-10-23 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few.
Download or read book Cardiac Cell Culture Technologies written by Zbigniew Brzozka and published by Springer. This book was released on 2017-11-21 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.
Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun (James) Li and published by Woodhead Publishing. This book was released on 2021-08-05 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic Devices for Biomedical Applications, Second Edition provides updated coverage on the fundamentals of microfluidics, while also exploring a wide range of medical applications. Chapters review materials and methods, microfluidic actuation mechanisms, recent research on droplet microfluidics, applications in drug discovery and controlled-delivery, including micro needles, consider applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds, and cover the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. This book is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. - Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores a wide range of medical applications - Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies - Details applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and its role in developing tissue scaffolds, and stem cell engineering
Download or read book Characterization and Application of Microfluidic Devices for Studying Cellular Migration and Chemotaxis written by Lyndon K. Lee and published by . This book was released on 2019 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic devices, often called "lab on a chip", have been utilized in the fields of physics, chemistry, and biology. In this thesis, the application of a microfluidic device as an in vitro assay to study cellular responses to biochemical and biophysical stimuli with a particular focus on the migration and invasion of cancer cells is discussed. The device consists of an array of well pairs joined by 10 microchannels. With device miniaturization and efficient use of space, a large number of experiments can therefore be performed simultaneously on a single platform. Polydimethylsiloxane (PDMS), an optically transparent silicone rubber, was used to fabricate the device and permitted direct visualization of cellular dynamics during migration with the aid of a phase contrast inverted microscope. Using finite element analysis, gradient formation simulations were conducted and showed that chemical gradients could be rapidly generated in zero-flow conditions and remained stable for several days without additional maintenance. This approach allowed for comparatively rapid and simple device analysis without the need for lengthy and resource-intensive fabrication processes. Several microchannel configurations were modeled to examine the steady-state gradient profile, and it was found that linear and nonlinear concentration gradients could be passively generated and maintained for extended durations. The utility of the device as a platform for studying chemotaxis was demonstrated using several different cell lines. Lung and breast carcinoma cells were found to migrate rapidly through the microchannels of the device towards a gradient of epidermal growth factor, a potent chemoattractant. Prostate cancer cells were exposed to gradients of soluble factors present in various organs, and it was found that the cells migrated preferentially to bone, brain, and testes lysates, Immunocytochemical analysis revealed that cells that entered the channels exhibited upregulation of vimentin, a mesenchymal marker. The role of the novel gene Migration and Invasion Enhancer 1 (MIEN1) in promoting cell migration in breast cancer was also investigated. Overexpression of MIEN1 was associated with increased migration. It was also found that phosphorylation of the canonical immunoreceptor tyrosine-based activation motif (ITAM) is critical to the function of MIEN1. The possible role of MIEN1 in mediating collective migration through the promotion of cell-cell adhesion was also investigated, and although MIEN1-overexpressing cells displayed an increased tendency to form aggregates, immunostaining for several classic cell-cell adhesion molecules revealed no difference between the two conditions. With this platform, multiple cell lines and experimental conditions can be implemented and monitored simultaneously. The microfluidic device can be used to study both chemotaxis and innate migratory potential of adherent cells. Furthermore, in situ molecular interrogation of cells by means of immunocytochemistry is demonstrated. With minimal modifications, this platform could be used to study a variety of cell migration-based phenomena.
Download or read book Microfluidics for Cellular Applications written by Gerardo Perozziello and published by Elsevier. This book was released on 2023-04-13 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics for Cellular Applications describes microfluidic devices for cell screening from a physical, technological and applications point-of-view, presenting a comparison with the cell microenvironment and conventional instruments used in medicine. Microfluidic technologies, protocols, devices for cell screening and treatment have reached an advanced state but are mainly used in research. Sections break them down into practical applications and conventional medical procedures and offers insights and analysis on how higher resolutions and fast operations can be reached. This is an important resource for those from an engineering and technology background who want to understand more and gain additional insights on cell screening processes. - Outlines the major applications of microfluidic devices in medicine and biotechnology - Assesses the major challenges of using microfluidic devices in terms of complexity of the control set-up, ease of use, integration capability, automation level, analysis throughput, content and costs - Describes the major fabrication techniques for assembling effective microfluidic devices for bioapplications
Download or read book Microfluidics and Nanofluidics written by Mohsen Sheikholeslami Kandelousi and published by BoD – Books on Demand. This book was released on 2018-08-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the present book, various applications of microfluidics and nanofluidics are introduced. Microfluidics and nanofluidics span a broad array of disciplines including mechanical, materials, and electrical engineering, surface science, chemistry, physics and biology. Also, this book deals with transport and interactions of colloidal particles and biomolecules in microchannels, which have great importance to many microfluidic applications, such as drug delivery in life science, microchannel heat exchangers in electronic cooling, and food processing industry. Furthermore, this book focuses on a detailed description of the thermal transport behavior, challenges and implications that involve the development and use of HTFs under the influence of atomistic-scale structures and industrial applications.
Download or read book Design Fabrication and Testing of a 3D Printer Based Microfluidic System written by Carlton A. McMullen and published by . This book was released on 2015 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: A pneumatically actuated PDMS based microfluidic devices were designed and fabricated by soft-lithography. Two types of molds were fabricated out of different material for this experiment. The first mold, (device 1), was fabricated from a sheet of Polymethyl methacrylate (PMMA) material, similar to Plexiglas. The device features were micro-engraved onto the face of the material. The second mold, (device 2), was fabricated from the use of fused deposition modeling (FDM) 3D printing. The pumping efficiency of the PDMS devices was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Tested at a driving pressure of 10psi, the flowrate for device 1 peaked at 75μL/min with a 7Hz actuation frequency before failing, while device 2 peaked at 498μL/min with a 15Hz actuation frequency. Using the techniques of rapid prototyping and fused deposition modelling a pneumatically actuated 3D printer based micro-pump and micro-mixer are fabricated. The devices were fabricated using a thermoplastic elastomer (TPE) material as an alternative material to the present polydimethylsiloxane (PDMS). The micro-pump{u2019}s fluid flow output was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Testing showed that a maximum flowrate of 1120μL/min was achieved at an actuation frequency of 10Hz with an applied driving pressure of 40psi. A qualitative mixing performance was conducted with the micro-mixer. The diffusion of two dyes was tested under an active mix and non-active mix condition. Testing showed that the active mixing condition resulted in a complete diffusion as opposed to the non-mixing condition which partially diffused. As a proof of concept for biological testing, E. coli and E.coli anti-bodies were mixes to measure the capturing efficiency. The results showed that the active mixing resulted in about 50% capturing efficiency as opposed to the non-mixing which resulted in 33% capturing efficiency.
Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Download or read book Fabrication Analysis Application and Characterization of Core containing Microparticles and Hydrophilic Microfluidic Devices Produced Via the Primary and in Situ Tertiary amine Catalyzed Michael Addition of Multifunctional Thiols to Multifunctional Acrylates written by Christopher O. Bounds and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Process Characterization of Fabricating Plastic Microfluidic Devices written by Carl Fredrickson and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Running chemical analysis in microfluidic devices, sometimes referred to as lab-on-a-chip technology, can increase accuracy while reducing the analysis time, sample size, and the amount of reagents consumed. While glass, silicon, and plastic can be used to make these devices, plastics have the advantage of lower costs, resulting in disposable devices that eliminate the risk of cross-contamination. Applications where these devices can be used include, but are not limited to, drug discovery, point-of-care or field testing, and forensic analysis.
Download or read book Microfluidic Cell Culture Systems written by Christopher Bettinger and published by William Andrew. This book was released on 2012-12-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products.
Download or read book Microfluidic Electrochemical Flow Cells written by Catherine Regina Cabrera and published by . This book was released on 2002 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Hidden in Plain Sight written by Albert Folch and published by MIT Press. This book was released on 2022-04-26 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stories behind essential microfluidic devices, from the inkjet printer to DNA sequencing chip. Hidden from view, microfluidics underlies a variety of devices that are essential to our lives, from inkjet printers to glucometers for the monitoring of diabetes. Microfluidics—which refers to the technology of miniature fluidic devices and the study of fluids at submillimeter levels—is invisible to most of us because it is hidden beneath ingenious user interfaces. In this book, Albert Folch, a leading researcher in microfluidics, describes the development and use of key microfluidic devices. He explains not only the technology but also the efforts, teams, places, and circumstances that enabled these inventions. Folch reports, for example, that the inkjet printer was one of the first microfluidic devices invented, and traces its roots back to nineteenth-century discoveries in the behavior of fluid jets. He also describes how rapid speed microfluidic DNA sequencers have enabled the sequencing of animal, plant, and microbial species genomes; organs on chips facilitate direct tests of drugs on human tissue, leapfrogging over the usual stage of animal testing; at-home pregnancy tests are based on clever microfluidic principles; microfluidics can be used to detect cancer cells in the early stages of metastasis; and the same technology that shoots droplets of ink on paper in inkjet printers enables 3D printers to dispense layers of polymers. Folch tells the stories behind these devices in an engaging style, accessible to nonspecialists. More than 100 color illustrations show readers amazing images of microfluids under the microscope.
Download or read book Analytical Instrumentation Handbook written by Jack Cazes and published by CRC Press. This book was released on 2004-11-30 with total page 1066 pages. Available in PDF, EPUB and Kindle. Book excerpt: Compiled by the editor of Dekker's distinguished Chromatographic Science series, this reader-friendly reference is as a unique and stand-alone guide for anyone requiring clear instruction on the most frequently utilized analytical instrumentation techniques. More than just a catalog of commercially available instruments, the chapters are wri
