Download or read book Extracellular Matrix derived Implants in Clinical Medicine written by Daniel L Mooradian and published by Woodhead Publishing. This book was released on 2016-05-18 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extracellular Matrix-Derived Implants in Clinical Medicine comprehensively covers the emergence of tissue engineering and regenerative medicine over the past few decades, along with discussions of continuous funding and research. The book provides a state-of-the-art review of this increasingly important technology and how it is translating from bench to bedside. Part One of the book looks at the historical use of human and animal tissues, focusing on the main application areas, including cardiovascular, hard and soft tissue engineering, and neurological, while Part Two examines the challenges in harvesting, processing, and manufacturing of extracellular matrices, with a final section reviewing the international regulatory environment and economics of tissue-based products. Addresses issues of tissue engineering and regenerative medicine from a biomaterials industry perspective Looks at the historical use of human and animal tissues, focusing on the main application areas, including cardiovascular, hard and soft tissue engineering, and neurological Examines the challenges in harvesting, processing, and manufacturing of extracellular matrices Reviews the international regulatory environment and economics of tissue-based products

Download or read book Immunomodulatory Biomaterials written by Stephen F. Badylak and published by Woodhead Publishing. This book was released on 2021-07-30 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomaterials have existed for millennia as mechanical replacement structures following disease or injury. Biomaterial design has changed markedly from structural support with an “inert immune profile as the primary objective to designs that elicit an integrative local tissue response and a pro-repair immune cell phenotype. Immunomodulatory Biomaterials: Regulating the Immune Response with Biomaterials to Affect Clinical Outcome offers a single, comprehensive reference on biomaterials for modulation of the host response, for materials scientists, tissue engineers and those working in regenerative medicine. This book details methods, materials and strategies designed to regulate the host immune response following surgical implantation and thus facilitate specific local cell infiltration and tissue deposition. There has been a dramatic transformation in our understanding of the role of the immune system, both innate and adaptive; these changes include recognition of the plasticity of immune cells, especially macrophages, cross-talk between the immune system and stem cells, and the necessity for in situ transition between inflammatory and regulatory immune cell phenotypes. The exploitation of these findings and the design and manufacture of new biomaterials is occurring at an astounding pace. There is currently no book directed at the interdisciplinary principles guiding the design, manufacture, testing, and clinical translation of biomaterials that proactively regulate the host tissue immune response. The challenge for academia, industry, and regulatory agencies to encourage innovation while assuring safety and maximizing efficacy has never been greater. Given the highly interdisciplinary requirements for the design, manufacture and use of immunomodulatory biomaterials, this book will prove a useful single resource across disciplines. Holistically covers the design, manufacture, testing, and clinical translation of biomaterials that proactively regulate the host tissue immune response Provides a single reference for understanding and utilizing the host response in biomaterials design An international collaboration of leading researchers in the field offering a novel insight into this fast-growing area

Download or read book Decellularized Extracellular Matrix written by Tetsuji Yamaoka and published by Royal Society of Chemistry. This book was released on 2019-12-05 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extracellular matrix (ECM) supports cells and regulates various cellular functions in our body. The native ECM promises to provide an excellent scaffold for regenerative medicine. In order to use the ECM as a scaffold in medicine, its cellular fractions need to be removed while retaining its structural and compositional properties. This process is called decellularization, and the resulting product is known as the decellularized extracellular matrix (dECM). This book focuses on the sources of dECM and its preparation, characterization techniques, fabrication, and applications in regenerative medicine and biological studies. Using this book, the reader will gain a good foundation in the field of ECM decellularization complemented with a broad overview of dECM characterization, ranging from structural observation and compositional assessment to immune responses against dECM and applications, ranging from microfabrication and 3D-printing to the application of tissue-derived dECM in vascular grafts and corneal tissue engineering etc. The book closes with a section dedicated to cultured cell dECM, a complementary technique of tissue-derived dECM preparation, for application in tissue engineering and regenerative medicine, addressing its use in stem cell differentiation and how it can help in the study of the tumor microenvironment as well as in clinical trials of peripheral nerve regeneration.

Download or read book Natural and Cell derived Matrices as Biomaterials in Bone Regeneration written by Jenna N. Harvestine and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: With over 2 million bone graft procedures ever year, bone is the second most transplanted material behind blood. However, limited tissue supply and post-operative concerns for donor site pain and morbidity motivate alternative strategies to drive bone regeneration. Mesenchymal stem cells (MSCs) are found within multiple tissue compartments in the postnatal organism, making them widely accessible for autologous cell therapy and a promising alternative to bone grafts. However, loss of osteogenic phenotype, poor cell viability, and insufficient vasculature infiltration after implantation challenge clinical application of tissue engineered constructs. Cell-derived extracellular matrix (ECM), is a biomimetic platform that provides a natural niche to promote trophic factor secretion, cell adhesion, survival, and osteogenic differentiation. We hypothesized a cell-secreted ECM would select endothelial and stem/progenitor cell populations from tissue aspirates to enhance cell survival and instruct cell function, resulting in improved osteogenic potential. First, we hypothesized MSC-derived ECM-coated scaffolds would enhance osteogenic differentiation and bone formation by culture expanded bone marrow-derived MSCs. Human MSCs cultured on ECM-coated scaffolds exhibited increased metabolic activity and decreased apoptosis compared to uncoated scaffolds. In short-term culture, MSCs on ECM-coated substrates secreted more proangiogenic factors while maintaining markers of osteogenic differentiation. Upon implantation, we detected improved survival of MSCs on ECM-coated scaffolds over 3 weeks. Histological evaluation revealed enhanced cellularization and osteogenic differentiation in ECM-coated scaffolds compared to controls. To determine whether the bioactivity of cell-secreted ECM was dependent on cell source, we assessed the osteogenic response of human stromal vascular fraction (SVF) on ECMs secreted by bone marrow-derived mesenchymal stem cells (MSCs) as well as adipose stem/stromal cells (ASCs) and human dermal fibroblasts (HDFs). Mass spectrometry analysis identified 278, 225, and 150 distinct proteins within MSC-, ASC-, and HDF-derived ECMs, respectively, suggesting that stromal cell-derived ECMs are more complex than HDF-derived ECMs which contained nearly 50% fewer distinct proteins. Regardless of ECM source, SVF deposited over 8- and 1.3-fold more calcium compared to tissue culture plastic (TCP) and collagen-coated controls, respectively. Flow cytometry confirmed that SVF cultured on ECM retained CD31 and CD34 positive cell populations better than TCP confirming a role for accessory cells in osteogenic differentiation by tissue aspirates. In addition to lipoaspirates, bone marrow aspirates (BMA) contain a rich source of cells for use in several clinical indications including bone repair. However, progenitor cells such as MSCs account for a small fraction of nucleated cells in BMA, requiring extensive in vitro culture for expansion. We hypothesized that cell-secreted ECM could be used to sequester MSCs and accessory cells from BMA for bone regeneration. To generate 3D implantable constructs, BMA was resuspended in media with or without type I collagen or ECM and injected into a perfusion bioreactor system. Compared to uncoated scaffolds, we observed a 30- and 143- fold increase in MSCs when fresh BMA was cultured on collagen- or ECM-coated scaffolds, respectively. Upon subcutaneous implantation, ECM-coated scaffolds promoted cell survival and early vascularization, yet bone formation was comparable across all implant groups, suggesting additional osteogenic cues are necessary. To determine if osteogenic pre-conditioning would improve the bone forming potential of BMA, cells were perfused on ECM-coated scaffold for either 20 hours or 14 days to generate naïve and pre-conditioned constructs, respectively. Naïve constructs secreted high levels of pro-angiogenic growth factors, while pre-conditioned cells exhibited an osteogenic phenotype and scaffolds contained more MSCs and endothelial cells. Constructs were implanted into 3.5 mm defects made in the calvariae of nude rats then monitored for vascular invasion and bone formation over 10 weeks. Vascular infiltration into pre-conditioned implants occurred rapidly over the first 14 days, resulting in greater vessel density compared to naïve implants which peaked at 28 days. Early advantages in vessel formation correlated to increased bone volume and tissue mineral density in pre-conditioned implants at 10 weeks confirming the synergy between angiogenesis and bone formation. Collectively, this dissertation describes the capacity of cell-secreted ECM to sequester, maintain, and direct osteogenic differentiation by two clinically investigated tissue aspirates for bone tissue engineering. Novel strategies that augment bone repair using autologous tissues address a critically unmet need and have direct implications to improve current standards of care at the forefront of orthopaedic regenerative medicine.

Download or read book Advances in Regenerative Medicine Role of Nanotechnology and Engineering Principles written by Venkatram Prasad Shastri and published by Springer Science & Business Media. This book was released on 2010-08-14 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the NATO Advanced Research Workshop (ARW) on “Nanoengineered Systems for Regenerative Medicine” that was organized under the auspices of the NATO Security through Science Program. I would like to thank NATO for supporting this workshop via a grant to the co-directors. The objective of ARW was to explore the various facets of regenerative me- cine and to highlight role of the “the nano-length scale” and “nano-scale systems” in defining and controlling cell and tissue environments. The development of novel tissue regenerative strategies require the integration of new insights emerging from studies of cell-matrix interactions, cellular signalling processes, developmental and systems biology, into biomaterials design, via a systems approach. The chapters in the book, written by the leading experts in their respective disciplines, cover a wide spectrum of topics ranging from stem cell biology, developmental biology, ce- matrix interactions, and matrix biology to surface science, materials processing and drug delivery. We hope the contents of the book will provoke the readership into developing regenerative medicine paradigms that combine these facets into cli- cally translatable solutions. This NATO meeting would not have been successful without the timely help of Dr. Ulrike Shastri, Sanjeet Rangarajan and Ms. Sabine Benner, who assisted in the organization and implementation of various elements of this meeting. Thanks are also due Dr. Fausto Pedrazzini and Ms. Alison Trapp at NATO HQ (Brussels, Belgium). The commitment and persistence of Ms.

Download or read book Principles of Regenerative Medicine written by Anthony Atala and published by Academic Press. This book was released on 2010-12-16 with total page 1203 pages. Available in PDF, EPUB and Kindle. Book excerpt: Virtually any disease that results from malfunctioning, damaged, or failing tissues may be potentially cured through regenerative medicine therapies, by either regenerating the damaged tissues in vivo, or by growing the tissues and organs in vitro and implanting them into the patient. Principles of Regenerative Medicine discusses the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions, such as diabetes, heart disease, liver disease, and renal failure. Key for all researchers and instituions in Stem Cell Biology, Bioengineering, and Developmental Biology The first of its kind to offer an advanced understanding of the latest technologies in regenerative medicine New discoveries from leading researchers on restoration of diseased tissues and organs

Download or read book Novel Biomaterials for Regenerative Medicine written by Heung Jae Chun and published by Springer. This book was released on 2018-10-24 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores in depth a wide range of new biomaterials that hold great promise for applications in regenerative medicine. The opening two sections are devoted to biomaterials designed to direct stem cell fate and regulate signaling pathways. Diverse novel functional biomaterials, including injectable nanocomposite hydrogels, electrosprayed nanoparticles, and waterborne polyurethane-based materials, are then discussed. The fourth section focuses on inorganic biomaterials, such as nanobioceramics, hydroxyapatite, and titanium dioxide. Finally, up-to-date information is provided on a wide range of smart natural biomaterials, ranging from silk fibroin-based scaffolds and collagen type I to chitosan, mussel-inspired biomaterials, and natural polymeric scaffolds. This is one of two books to be based on contributions from leading experts that were delivered at the 2018 Asia University Symposium on Biomedical Engineering in Seoul, Korea – the companion book examines in depth the latest enabling technologies for regenerative medicine.

Download or read book Musculoskeletal Tissue Regeneration written by William S. Pietrzak and published by Springer Science & Business Media. This book was released on 2008-04-11 with total page 647 pages. Available in PDF, EPUB and Kindle. Book excerpt: The repair of musculoskeletal tissue is a vital concern of all surgical specialties, orthopedics and related disciplines. Written by recognized experts, this book aims to provide both basic and advanced knowledge of the newer methodologies being developed and introduced to the clinical arena. A valuable resource for researchers, developers, and clinicians, the book presents a foundation to propel the technology and integration of the current state of knowledge into the 21st century.

Download or read book In Situ Tissue Regeneration written by Sang Jin Lee and published by Academic Press. This book was released on 2016-07-17 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Situ Tissue Regeneration: Host Cell Recruitment and Biomaterial Design explores the body’s ability to mobilize endogenous stem cells to the site of injury and details the latest strategies developed for inducing and supporting the body’s own regenerating capacity. From the perspective of regenerative medicine and tissue engineering, this book describes the mechanism of host cell recruitment, cell sourcing, cellular and molecular roles in cell differentiation, navigational cues and niche signals, and a tissue-specific smart biomaterial system that can be applied to a wide range of therapies. The work is divided into four sections to provide a thorough overview and helpful hints for future discoveries: endogenous cell sources; biochemical and physical cues; smart biomaterial development; and applications. Explores the body’s ability to mobilize endogenous stem cells to the site of injury Details the latest strategies developed for inducing and supporting the body’s own regenerating capacity Presents smart biomaterials in cell-based tissue engineering applications—from the cell level to applications—in the first unified volume Features chapter authors and editors who are authorities in this emerging field Prioritizes a discussion of the future direction of smart biomaterials for in situ tissue regeneration, which will affect an emerging and lucrative industry

Download or read book Biomaterials for Skin Repair and Regeneration written by Elena Garcia-Gareta and published by Woodhead Publishing. This book was released on 2019-06-05 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomaterials for Skin Repair and Regeneration examines a range of materials and technologies used for regenerating or repairing skin. With a strong focus on biomaterials and scaffolds, the book also examines the testing and evaluation pathway for human clinical trials. Beginning by introducing the fundamentals on skin tissue, the book goes on to describe contemporary technology used in skin repair as well as currently available biomaterials suitable for skin tissue repair and regeneration. Skin tissue engineering and the ideal requirements to take into account when developing skin biomaterials are discussed, followed by information on the individual materials used for skin repair and regeneration. As evaluation of biomaterials in animal models is mandatory before proceeding into human clinical trials, the book also examines the different animal models available. With a strong focus on materials, engineering, and application, this book is a valuable resource for materials scientists, skin biologists, and bioengineers with an interest in tissue engineering, regeneration, and repair of skin. Provides an understanding of basic skin biology Comprehensively examines a variety of biomaterial approaches Looks at animal models for the evaluation of biomaterial-based skin constructs

Download or read book Mimicking the Extracellular Matrix written by Gregory H. Hudalla and published by Royal Society of Chemistry. This book was released on 2015-11-18 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mimicking the Extracellular Matrix approaches this topic from both basic science and practical engineering perspectives. Suitable for undergraduates, postgraduates, and academics, this text aims to unify the current knowledge of ECM biology and matrix-mimicking biomaterials.

Download or read book Bone Tissue Engineering written by Jeffrey O. Hollinger and published by CRC Press. This book was released on 2004-10-14 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t

Download or read book Host Response to Biomaterials written by Stephen F. Badylak and published by Academic Press. This book was released on 2015-05-08 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Host Response to Biomaterials: The Impact of Host Response on Biomaterial Selection explains the various categories of biomaterials and their significance for clinical applications, focusing on the host response to each biomaterial. It is one of the first books to connect immunology and biomaterials with regard to host response. The text also explores the role of the immune system in host response, and covers the regulatory environment for biomaterials, along with the benefits of synthetic versus natural biomaterials, and the transition from simple to complex biomaterial solutions. Fields covered include, but are not limited to, orthopaedic surgery, dentistry, general surgery, neurosurgery, urology, and regenerative medicine. Explains the various categories of biomaterials and their significance for clinical applications Contains a range of extensive coverage, including, but not limited to, orthopedic, surgery, dental, general surgery, neurosurgery, lower urinary tract, and regenerative medicine Includes regulations regarding combination devices

Download or read book Injectable Biomaterials written by Brent Vernon and published by Elsevier. This book was released on 2011-01-24 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Novel injectable materials for non-invasive surgical procedures are becoming increasingly popular. An advantage of these materials include easy deliverability into the body, however the suitability of their mechanical properties must also be carefully considered. Injectable biomaterials covers the materials, properties and biomedical applications of injectable materials, as well as novel developments in the technology.Part one focuses on materials and properties, with chapters covering the design of injectable biomaterials as well as their rheological properties and the mechanical properties of injectable polymers and composites. Part two covers the clinical applications of injectable biomaterials, including chapters on drug delivery, tissue engineering and orthopaedic applications as well as injectable materials for gene delivery systems. In part three, existing and developing technologies are discussed. Chapters in this part cover such topics as environmentally responsive biomaterials, injectable nanotechnology, injectable biodegradable materials and biocompatibility. There are also chapters focusing on troubleshooting and potential future applications of injectable biomaterials.With its distinguished editor and international team of contributors, Injectable biomaterials is a standard reference for materials scientists and researchers working in the biomaterials industry, as well as those with an academic interest in the subject. It will also be beneficial to clinicians. Comprehensively examines the materials, properties and biomedical applications of injectable materials, as well as novel developments in the technology Reviews the design of injectable biomaterials as well as their rheological properties and the mechanical properties of injectable polymers and composites Explores clinical applications of injectable biomaterials, including drug delivery, tissue engineering, orthopaedic applications and injectable materials for gene delivery systems

Download or read book Extracellular Matrix Assembly and Structure written by Peter D. Yurchenco and published by Academic Press. This book was released on 2013-10-22 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: The complex and critical process of extracellular matrix (ECM) assembly is described in this book. Assembly may involve molecules interacting with molecules of the same matrix class, such as in collagen, or interactions between different ECM molecules, such as in basement membranes. The text shows how this is driven by structural information within the matrix monomer. This information will be of interest to cell, developmental, and molecular biologists, biochemists, biophysicists, and biomedical researchers involved in macromolecular assembly, biological macromolecules, and extracellular matrix. Addresses assembly of most of the known classes of extracellular matrix macromolecules Discusses higher order structures produced by ECM Gives important concepts in ECM and cell-matrix interactions, Protein structure and protein-protein interactions, Development and tissue remodeling

Download or read book Biomaterials for Implants and Scaffolds written by Qing Li and published by Springer. This book was released on 2016-11-30 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the latest, cutting-edge advances in implantable biomaterials. It brings together a class of advanced biomaterials in two highly active research areas, namely implants and tissue scaffolds, to underline their respective functional requirements for further development. It is unique in providing a full range of methodological procedures, including materials syntheses, characterisation, cellular tests and mathematical modelling. Covering metallic, ceramic, polymeric and composite materials commonly used in biological applications and clinical therapeutics, it is a valuable resource for anyone wanting to further their understanding of the latest developments in implantable biomaterials. Focusing on biomedical applications in implants and scaffolds, it provides methodological guides to this rapidly growing field. Qing Li and Yiu-Wing Mai are both professors at the University of Sydney, School of Aerospace, Mechanical and Mechatronic Engineering.

Download or read book Stem Cell Regulators written by and published by Academic Press. This book was released on 2011-11-23 with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1943, Vitamins and Hormones is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms. Under the capable and qualified editorial leadership of Dr. Gerald Litwack, Vitamins and Hormones continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines. This volume focuses on stem cell regulators. Longest running series published by Academic Press Contributions by leading international authorities